Version 1
: Received: 19 July 2023 / Approved: 20 July 2023 / Online: 21 July 2023 (10:09:28 CEST)
How to cite:
Yadav, C.K.; KC, S.; Thapa, S.; Kandel, K.; Shrestha, L.; Palikhey, A.; Lamsal, A. In Vitro and In Silico Analysis of Alpha-Amylase Inhibitory Activity of Ethanolic Extract of Adhatoda vasica Leaves. Preprints2023, 2023071457. https://doi.org/10.20944/preprints202307.1457.v1
Yadav, C.K.; KC, S.; Thapa, S.; Kandel, K.; Shrestha, L.; Palikhey, A.; Lamsal, A. In Vitro and In Silico Analysis of Alpha-Amylase Inhibitory Activity of Ethanolic Extract of Adhatoda vasica Leaves. Preprints 2023, 2023071457. https://doi.org/10.20944/preprints202307.1457.v1
Yadav, C.K.; KC, S.; Thapa, S.; Kandel, K.; Shrestha, L.; Palikhey, A.; Lamsal, A. In Vitro and In Silico Analysis of Alpha-Amylase Inhibitory Activity of Ethanolic Extract of Adhatoda vasica Leaves. Preprints2023, 2023071457. https://doi.org/10.20944/preprints202307.1457.v1
APA Style
Yadav, C.K., KC, S., Thapa, S., Kandel, K., Shrestha, L., Palikhey, A., & Lamsal, A. (2023). In Vitro and In Silico Analysis of Alpha-Amylase Inhibitory Activity of Ethanolic Extract of Adhatoda vasica Leaves. Preprints. https://doi.org/10.20944/preprints202307.1457.v1
Chicago/Turabian Style
Yadav, C.K., Anjan Palikhey and Ashish Lamsal. 2023 "In Vitro and In Silico Analysis of Alpha-Amylase Inhibitory Activity of Ethanolic Extract of Adhatoda vasica Leaves" Preprints. https://doi.org/10.20944/preprints202307.1457.v1
Abstract
Background: Diabetic patients are more likely to experience morbidity and mortality as a result of microvascular complications such as retinopathy, neuropathy, nephropathy, and stroke. There are many synthetic anti-diabetic agents available which are expensive and have undesirable pathological effects. Thus, it is essential to look for cost-effective, natural, and safe antidiabetic agents. The aim of this study was to screen phytoconstituent and evaluate the in-vitro and in-silico α-amylase inhibitory activity of ethanolic extract of Adhatoda vasica leaves. Method: The extraction of Adhatoda vasica leaves was performed with ethanol through Soxhlet extraction process. Different concentrations (0.1 mg/ml to 1 mg/ml) of ethanolic extract, Acarbose, and Sitagliptin, were prepared and all concentrations were evaluated for α-amylase inhibitory activity through the spectrophotometric method. Molecular docking (AutodockVina 1.2.0) and toxicity profiling (SToPToX web server) studies were performed. Results: The plant extract showed highest inhibition of α-amylase (56.763±0.0035) at a concentration of 1 mg/ml. This inhibitory activity was supported by the in-silico study. Vasicoline (C5) and quercetin (C9), active constitute of plant Adhatoda vasica, showed the best binding energy of 8.3 and 8.0 Kcal/mol respectively with α-amylase enzyme (PDBID: 4W93). Toxicity study revealed the safety profile of plant extract. Conclusion: It was concluded that Adhatoda vasica leaves possess some bioactive compounds which are responsible for controlling blood glucose levels and its identification, purification, and isolation may lead to the development of newer therapeutic agent with lesser side effects.
Chemistry and Materials Science, Medicinal Chemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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