Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Nanoribbon Biosensor for Detection of microRNA Associated with Prostate Cancer in Humans

Yuri Ivanov
* ORCID logo ,
Kristina Malsagova
ORCID logo ,
Kristina Goldaeva
,
Svetlana Kapustina
ORCID logo ,
Tatyana Pleshakova
ORCID logo ,
Vladimir Popov
ORCID logo ,
Andrey Kozlov
,
Rafael Galiullin
,
Ivan Shumov
,
Angelina Vinogradova
ORCID logo ,
Dmitry Enikeev
,
Natalia Potoldykova
,
Vadim Ziborov
,
Oleg Petrov
,
Alexander Dolgoborodov
ORCID logo ,
Alexander Glukhov
,
Sergey Novikov
,
Victoria Grabezhova
,
Evgeniy Yushkov
,
Vladimir Konev
,
Oleg Kovalev
ORCID logo ,
Alexander Archakov
Version 1 : Received: 7 July 2023 / Approved: 10 July 2023 / Online: 11 July 2023 (05:08:14 CEST)

A peer-reviewed article of this Preprint also exists.

Ivanov, Y.D.; Malsagova, K.A.; Goldaeva, K.V.; Kapustina, S.I.; Pleshakova, T.O.; Popov, V.P.; Kozlov, A.F.; Galiullin, R.A.; Shumov, I.D.; Enikeev, D.V.; Potoldykova, N.V.; Ziborov, V.S.; Petrov, O.F.; Dolgoborodov, A.Y.; Glukhov, A.V.; Novikov, S.V.; Grabezhova, V.K.; Yushkov, E.S.; Konev, V.A.; Kovalev, O.B.; Archakov, A.I. Nanoribbon Biosensor-Based Detection of microRNA Markers of Prostate Cancer. Sensors 2023, 23, 7527. Ivanov, Y.D.; Malsagova, K.A.; Goldaeva, K.V.; Kapustina, S.I.; Pleshakova, T.O.; Popov, V.P.; Kozlov, A.F.; Galiullin, R.A.; Shumov, I.D.; Enikeev, D.V.; Potoldykova, N.V.; Ziborov, V.S.; Petrov, O.F.; Dolgoborodov, A.Y.; Glukhov, A.V.; Novikov, S.V.; Grabezhova, V.K.; Yushkov, E.S.; Konev, V.A.; Kovalev, O.B.; Archakov, A.I. Nanoribbon Biosensor-Based Detection of microRNA Markers of Prostate Cancer. Sensors 2023, 23, 7527.

Abstract

Oncological changes in the prostate are a common cause of death among elderly men. Malignant cells are difficult to identify at early stages, which are asymptomatic. The patient often seeks medical assistance when the disease has already progressed, and the treatment is less effective. Early diagnosis of prostate cancer (PC) is thus one of the priority tasks of modern urology and healthcare in general. The aim of this study is the detection of microRNAs (miRNAs), which are known to be associated with PC. The detection of the target miRNAs in human plasma samples has been performed with a nanoribbon biosensor system, which was based on "silicon-on-insulator" structures (SOI-NR biosensor). In order to provide biospecific detection of the target miRNAs, the surface of individual nanoribbons has been modified with DNA oligonucleotide probes (oDNA probes), whose nucleotide sequences were complementary to those of the target miRNAs. The high detection sensitivity has been demonstrated with the use of model oDNAs, which are complementary to nanoribbon-immobilized oDNA probes, in buffer solutions. The sensitivity threshold was 1.1 × 10-17 M. The successful detection of target miRNAs, isolated from real plasma samples of PC patients, has also been demonstrated. We believe that the development of highly sensitive nanotechnology-based biosensors for PC markers is a step towards personalized medicine.

Keywords

prostate cancer; microRNA; silicon-on-insulator; nanoribbon; biomarker

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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