PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
A Sustainable Green Synthesize of Nanocomposite of Silver Nanoparticles Functionalized with Pectin using Carpesium nepalense for Different Therapeutic Application: A Mechanistic Approach
Version 1
: Received: 7 June 2023 / Approved: 7 June 2023 / Online: 7 June 2023 (14:22:20 CEST)
How to cite:
Burki, S.; Arif Asghar, M.; Ali, I.; Asghar, M.A.; Burki, Z.G.; Javed, I.; Ullah, R. A Sustainable Green Synthesize of Nanocomposite of Silver Nanoparticles Functionalized with Pectin using Carpesium nepalense for Different Therapeutic Application: A Mechanistic Approach. Preprints2023, 2023060564. https://doi.org/10.20944/preprints202306.0564.v1
Burki, S.; Arif Asghar, M.; Ali, I.; Asghar, M.A.; Burki, Z.G.; Javed, I.; Ullah, R. A Sustainable Green Synthesize of Nanocomposite of Silver Nanoparticles Functionalized with Pectin using Carpesium nepalense for Different Therapeutic Application: A Mechanistic Approach. Preprints 2023, 2023060564. https://doi.org/10.20944/preprints202306.0564.v1
Burki, S.; Arif Asghar, M.; Ali, I.; Asghar, M.A.; Burki, Z.G.; Javed, I.; Ullah, R. A Sustainable Green Synthesize of Nanocomposite of Silver Nanoparticles Functionalized with Pectin using Carpesium nepalense for Different Therapeutic Application: A Mechanistic Approach. Preprints2023, 2023060564. https://doi.org/10.20944/preprints202306.0564.v1
APA Style
Burki, S., Arif Asghar, M., Ali, I., Asghar, M.A., Burki, Z.G., Javed, I., & Ullah, R. (2023). A Sustainable Green Synthesize of Nanocomposite of Silver Nanoparticles Functionalized with Pectin using <em>Carpesium nepalense</em> for Different Therapeutic Application: A Mechanistic Approach. Preprints. https://doi.org/10.20944/preprints202306.0564.v1
Chicago/Turabian Style
Burki, S., Ibrahim Javed and Riaz Ullah. 2023 "A Sustainable Green Synthesize of Nanocomposite of Silver Nanoparticles Functionalized with Pectin using <em>Carpesium nepalense</em> for Different Therapeutic Application: A Mechanistic Approach" Preprints. https://doi.org/10.20944/preprints202306.0564.v1
Abstract
Present study reports the green synthesis of pectin fabricated silver-based nanocomposite (Pectin-AgNPs) with Carpesiumnepalense leaves extract and evaluated their bactericidal kinetics, in-vivo hepatoprotective and cytotoxic potentials with their possible mechanisms. GC/MS and LC/MS analysis indicated the presence of different new phytochemicals constituents in the plant extract. The physicochemical characterization of Pectin-AgNPs by UV/Vis, SEM, DLS, FTIR and EDX techniques showed the spherical and uniform size range synthesis of nanocomposite i.e. 50-110 nm. The highly significant (P<0.005) antibacterial activity was found against all tested four bacterial strains with the ZIs of 24.8 to 27.2 mm. Significant damage in bacterial cell membrane was also observed in AFM study after treated with Pectin-AgNPs. At the doses of 0.05 mg/kg, nanocomposite showed highly significant (P<0.005)hepatoprotective activity in biochemical and histopathology analysis as compared to CCL4 administered control group. The ameliorative effects of Pectin-AgNPs were observed in PCR analysis on both GAPDH and PPARs genes expression and its potential to restore gene alterations caused by CCl4 intoxication.Pectin-AgNPs produced cytotoxic activity against HeLa cell lines at higher doses with the LC50 of 223.7 µg/mL. The current findings demonstrate positive attributes of pectin fabricated AgNPs as a promising antibacterial, hepatoprotective and cytotoxic agent.
Keywords
Green Synthesis; Pectin functionalized silver nanoparticles; Antibacterial activity; Hepatoprotective
Subject
Chemistry and Materials Science, Medicinal Chemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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