Version 1
: Received: 29 May 2023 / Approved: 30 May 2023 / Online: 30 May 2023 (10:59:51 CEST)
How to cite:
Ghasim, H.; Rouini, M.; Safari, S.; Larti, F.; Khoshayand, M.; Gholami, K.; Neyshaburinezhad, N.; Gloor, Y.; Daali, Y.; H. Ardakani, Y. Impact of Obesity and Bariatric Surgery on Metabolic Enzymes and P-glycoprotein Activity using Geneva Cocktail Approach. Preprints2023, 2023052120. https://doi.org/10.20944/preprints202305.2120.v1
Ghasim, H.; Rouini, M.; Safari, S.; Larti, F.; Khoshayand, M.; Gholami, K.; Neyshaburinezhad, N.; Gloor, Y.; Daali, Y.; H. Ardakani, Y. Impact of Obesity and Bariatric Surgery on Metabolic Enzymes and P-glycoprotein Activity using Geneva Cocktail Approach. Preprints 2023, 2023052120. https://doi.org/10.20944/preprints202305.2120.v1
Ghasim, H.; Rouini, M.; Safari, S.; Larti, F.; Khoshayand, M.; Gholami, K.; Neyshaburinezhad, N.; Gloor, Y.; Daali, Y.; H. Ardakani, Y. Impact of Obesity and Bariatric Surgery on Metabolic Enzymes and P-glycoprotein Activity using Geneva Cocktail Approach. Preprints2023, 2023052120. https://doi.org/10.20944/preprints202305.2120.v1
APA Style
Ghasim, H., Rouini, M., Safari, S., Larti, F., Khoshayand, M., Gholami, K., Neyshaburinezhad, N., Gloor, Y., Daali, Y., & H. Ardakani, Y. (2023). Impact of Obesity and Bariatric Surgery on Metabolic Enzymes and P-glycoprotein Activity using Geneva Cocktail Approach. Preprints. https://doi.org/10.20944/preprints202305.2120.v1
Chicago/Turabian Style
Ghasim, H., Youssef Daali and Yalda H. Ardakani. 2023 "Impact of Obesity and Bariatric Surgery on Metabolic Enzymes and P-glycoprotein Activity using Geneva Cocktail Approach" Preprints. https://doi.org/10.20944/preprints202305.2120.v1
Abstract
The inter-individual variability of CYP450s enzyme activity may be reduced by comparing the effects of bariatric surgery on CYP-mediated drug elimination in comparable patients before and after surgery. The current research will use a low-dose phenotyping cocktail to simultaneously evaluate the activities of six CYP isoforms and P-gp.The results showed that following weight reduction after surgery, the activity of all enzymes increased compared to the obese period, which was statistically significant in the case of CYP3A, CYP2B6, CYP2C9, and CYP1A2. Furthermore, the activity of P-gp after surgery decreased without reaching a statistical significance (p-value>0.05). Obese individuals had decreased CYP3A and CYP2D6 activity than the control group, albeit only CYP3A was statistically important. In addition, there was a trend to increase activity for CYP1A2, CYP2B6, CYP2C9, and CYP2C19 in obese patients compared to the control group, without reaching statistical insignificance (p-value≥0.05). After six months (at least), all enzymes and the P-gp pump activity were significantly higher than the control group except for CYP2D6.Ultimately, a greater comprehension of phenoconversion can aid in altering the patient's treatment. Further studies are required to confirm the changes in the metabolic ratios of probes after bariatric surgery to demonstrate the findings' clinical application. As a result, the effects of inflammation-induced phenoconversion on medication metabolism may differ greatly across persons and drug CYP pathways. It is essential to apply these results to the clinic to recommend dose adjustments.
Keywords
Phenoconversion; Bariatric surgery; CYP450 Phenotype and Genotype; Cocktail; Personalized medicine
Subject
Medicine and Pharmacology, Endocrinology and Metabolism
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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