Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Dendritic Mesoporous Organosilica Nanoparticles with Photosensitizers for Cell Imaging, siRNA Delivery and Protein Loading

Haneen Omar
* ORCID logo ,
Sara Jakimoska
,
Julia Guillot
,
Edreese Alsharaeh
ORCID logo ,
Clarence Charnay
ORCID logo ,
Frédérique Cunin
,
Aurélie Bessière
ORCID logo ,
Jean-Olivier Durand
ORCID logo ,
Laurence Raehm
,
Laure Lichon
,
Mélanie Onofre
,
Magali Gary-Bobo
ORCID logo
Version 1 : Received: 25 May 2023 / Approved: 26 May 2023 / Online: 26 May 2023 (10:47:14 CEST)

A peer-reviewed article of this Preprint also exists.

Omar, H.; Jakimoska, S.; Guillot, J.; Alsharaeh, E.; Charnay, C.; Cunin, F.; Bessière, A.; Durand, J.-O.; Raehm, L.; Lichon, L.; Onofre, M.; Gary-Bobo, M. Dendritic Mesoporous Organosilica Nanoparticles with Photosensitizers for Cell Imaging, siRNA Delivery and Protein Loading. Molecules 2023, 28, 5335. Omar, H.; Jakimoska, S.; Guillot, J.; Alsharaeh, E.; Charnay, C.; Cunin, F.; Bessière, A.; Durand, J.-O.; Raehm, L.; Lichon, L.; Onofre, M.; Gary-Bobo, M. Dendritic Mesoporous Organosilica Nanoparticles with Photosensitizers for Cell Imaging, siRNA Delivery and Protein Loading. Molecules 2023, 28, 5335.

Abstract

Dendritic Mesoporous Organosilica Nanoparticles (DMON) are a new class of biodegradable nanoparticles suitable for biomolecules delivery. In order to escape from the endosomes-lysosomes and to deliver biomolecules in the cytoplasm of cells, we studied photochemical internalization (PCI) and photodynamic therapy (PDT) of DMON. We added photosensitizers in the framework of DMON. DMON were also loaded with siRNA or FVIII factor protein. We made four formulations with four different photosensitizers, The photosensitizers allowed to perform imaging of DMON in cancer cells, but the presence of the tetrasulfide bond in the framework of DMON quenched the formation of singlet oxygen. Fortunately one formulation allowed to efficiently deliver proapoptotic siRNA in MCF-7 cancer cells leading to 31% of cancer cell death, without irradiation. For FVIII protein, it was loaded in two formulations with drug loading capacities (DLC) up to 25%. In conclusion DMON are versatile nanoparticles which allowed to load siRNA and to deliver it in cancer cells, and to load FVIII protein with good DLC. Due to the presence of tetrasulfide, it was not possible to perform PDT and PCI.

Keywords

Dendritic Mesoporous Organisilica Nanoparticles; siRNA; FVIII Factor

Subject

Chemistry and Materials Science, Materials Science and Technology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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