Version 1
: Received: 2 May 2023 / Approved: 4 May 2023 / Online: 4 May 2023 (07:17:52 CEST)
How to cite:
Benyahia, H.; Parola, P.; ALMERAS, L. Evolution of MALDI-TOF MS Profiles From Lice and Fleas Preserved in Alcohol Over Time. Preprints2023, 2023050208. https://doi.org/10.20944/preprints202305.0208.v1
Benyahia, H.; Parola, P.; ALMERAS, L. Evolution of MALDI-TOF MS Profiles From Lice and Fleas Preserved in Alcohol Over Time. Preprints 2023, 2023050208. https://doi.org/10.20944/preprints202305.0208.v1
Benyahia, H.; Parola, P.; ALMERAS, L. Evolution of MALDI-TOF MS Profiles From Lice and Fleas Preserved in Alcohol Over Time. Preprints2023, 2023050208. https://doi.org/10.20944/preprints202305.0208.v1
APA Style
Benyahia, H., Parola, P., & ALMERAS, L. (2023). Evolution of MALDI-TOF MS Profiles From Lice and Fleas Preserved in Alcohol Over Time. Preprints. https://doi.org/10.20944/preprints202305.0208.v1
Chicago/Turabian Style
Benyahia, H., Philippe Parola and Lionel ALMERAS. 2023 "Evolution of MALDI-TOF MS Profiles From Lice and Fleas Preserved in Alcohol Over Time" Preprints. https://doi.org/10.20944/preprints202305.0208.v1
Abstract
MALDI-TOF is now considered as a relevant tool for the identification of arthropods, including lice and fleas. However, the duration and conditions of storage, such as in ethanol, which is frequently used to preserve these both ectoparasites, could impede their classification. The purpose of the present study was to assess the stability of MS profiles from Pediculus humanus corporis lice and Ctenocephalides felis fleas preserved in alcohol from one to four years and kinetically submitted to MALDI-TOF MS. A total of 469 cephalothoraxes from lice (n=170) and fleas (n=299) were tested. The reproducibility of the MS profiles was estimated based on the log score values (LSVs) obtained for query profiles compared to the reference profiles included in the MS database. Only MS spectra from P. humanus corporis and Ct. felis stored in alcohol for less than one year were included in the reference MS database. Approximately 75% of MS spectra from lice (75.2%, 94/125) and fleas (74.4%, 122/164) specimens stored in alcohol during 12 to 48 months, queried against the reference MS database, obtained a relevant identification. An accurate analysis revealed a significant decrease in the proportion of identification for both species stored for more than 22 months in alcohol. It was hypothesized that incomplete drying was responsible of MS spectra variations. Then, 45 lice and 60 fleas were subjected to longer drying periods from 12 to 24 hours. The increase in the drying period improved the proportion of relevant identification for lice (95%) and fleas (80%). This study highlighted that a correct rate of identification by MS could be obtained for lice and fleas preserved in alcohol for up to four years, on the condition that the drying period was sufficiently long for accurate identification.
Keywords
arthropods; MALDI-TOF MS; duration of ethanol storing; species identification
Subject
Biology and Life Sciences, Insect Science
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
