Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Neurobehavioral Effects of Fermented Rice Bran Extract in Zebrafish Larvae Model

Jin sil Chae
ORCID logo ,
Seong soon Kim
,
Kyu-Seok Hwang
ORCID logo ,
Hyemin Kan
,
Jung Yoon Yang
,
Byunghoi Lee
,
Dae-Seop Shin
ORCID logo ,
ByoungHee Park
ORCID logo ,
Myung Ae Bae
*
Version 1 : Received: 28 April 2023 / Approved: 28 April 2023 / Online: 28 April 2023 (07:10:17 CEST)

A peer-reviewed article of this Preprint also exists.

Chae, J.S.; Kim, S.S.; Hwang, K.-S.; Kan, H.; Yang, J.Y.; Lee, B.; Shin, D.-S.; Park, B.; Bae, M.A. Neurobehavioral Effects of Fermented Rice Bran Extract in Zebrafish Larvae Model. Fermentation 2023, 9, 479. Chae, J.S.; Kim, S.S.; Hwang, K.-S.; Kan, H.; Yang, J.Y.; Lee, B.; Shin, D.-S.; Park, B.; Bae, M.A. Neurobehavioral Effects of Fermented Rice Bran Extract in Zebrafish Larvae Model. Fermentation 2023, 9, 479.

Abstract

Rice bran (RB) is a promising food ingredient that can improve biological function. In this study, we investigated the effects of RB, both unfermented (RB30) and fermented (RBF30) with five different microorganisms on the neurobehavioral activity in zebrafish larvae. Analytical methods such as UV spectrum and MS scan were performed. Interestingly, niacin content, which is known to improve brain functions such as cognition and emotion, was found to be higher in RBF30 than in RB30. Furthermore, niacin content was highly increased in the RBF30-exposed fish, compared to those in control fish. Therefore, we profiled behavioral patterns and various neurochemistry in zebrafish larvae following supplementation with RBF30 as well as performed calcium imaging on Tg (huC:GAL4-VP16);(UAS:GCaMP7a) zebrafish larvae to determine the correlation of neural activity. RBF30 revealed greater stimulation of locomotor activity without negatively affecting decision-making behavior in zebrafish larvae, as compared to RB30 or niacin. Its behavioral activation is mainly linked with the elevations of neural activity and several neurochemicals such as serotonergic and dopaminergic systems that are implicated in the control of anxiety and stress. Taken together, these results suggest that RBF30 could be a food material that improve the behavioral health by modulating neural activity and brain neurochemistry.

Keywords

Rice bran fermentation; Niacin; Behavior; Neural activity; Neurochemistry; Zebrafish

Subject

Biology and Life Sciences, Neuroscience and Neurology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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