Transcriptomic and Proteomic Analysis Reveals the Potential Role of RBMS1 in Adipogenesis and Adipocyte Metabolism

Ghida Dairi; Saeed Al Mahri; Hicham Benabdelkamel; Assim A. Alfadda; Abdulrahman A. Alswaji; Mamoon Rashid; Shuja Shafi Malik; Jahangir Iqbal; Rizwan Ali; Maria Al Ibrahim; Khalid A. Al-Regaiey; Sameer Mohammad

doi:10.20944/preprints202304.0439.v1

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preprints.org > biology and life sciences > biochemistry and molecular biology > doi: 10.20944/preprints202304.0439.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Transcriptomic and Proteomic Analysis Reveals the Potential Role of RBMS1 in Adipogenesis and Adipocyte Metabolism

Abdulrahman A. Alswaji

Version 1 : Received: 16 April 2023 / Approved: 17 April 2023 / Online: 17 April 2023 (10:09:50 CEST)

A peer-reviewed article of this Preprint also exists.

Dairi, G.; Al Mahri, S.; Benabdelkamel, H.; Alfadda, A.A.; Alswaji, A.A.; Rashid, M.; Malik, S.S.; Iqbal, J.; Ali, R.; Al Ibrahim, M.; Al-Regaiey, K.; Mohammad, S. Transcriptomic and Proteomic Analysis Reveals the Potential Role of RBMS1 in Adipogenesis and Adipocyte Metabolism. Int. J. Mol. Sci. 2023, 24, 11300. Dairi, G.; Al Mahri, S.; Benabdelkamel, H.; Alfadda, A.A.; Alswaji, A.A.; Rashid, M.; Malik, S.S.; Iqbal, J.; Ali, R.; Al Ibrahim, M.; Al-Regaiey, K.; Mohammad, S. Transcriptomic and Proteomic Analysis Reveals the Potential Role of RBMS1 in Adipogenesis and Adipocyte Metabolism. Int. J. Mol. Sci. 2023, 24, 11300.

Abstract

Adipocytes play a critical role in maintaining a healthy systemic metabolism by storing and releasing energy in the form of fat and helping to regulate glucose and lipid levels in the body. Adipogenesis is the process through which pre-adipocytes are differentiated into mature adipocytes. It is a complex process involving various transcription factors and signaling pathways. Dysregulation of adipogenesis has been implicated in the development of obesity and metabolic disorders. Therefore, understanding the mechanisms that regulate adipogenesis and the factors that contribute to its dysregulation may provide insights into the prevention and treatment of these conditions. RNA Binding Motif Single Stranded Interacting Protein 1 (RBMS1) is a protein that binds to RNA and plays a critical role in various cellular processes such as alternative splicing, mRNA stability, and translation. The RBMS1 polymorphism has been shown to be associated with obesity and Type 2 diabetes, but the role of RBMS1 in adipose metabolism and adipogenesis is not known. We show that RBMS1 is highly expressed during the early phase of differentiation of the murine adipocyte cell line 3T3-L1 and is significantly upregulated in adipose tissue depots and adipocytes of high-fat-fed mice, implying a possible role in adipogenesis and adipose metabolism. Knockdown of RBMS1 in pre-adipocytes impacted the differentiation process and reduced the expression of some of the key adipogenic markers. Transcriptomic and proteomic analysis indicated that RBMS1 depletion affected the expression of several genes involved in major metabolic processes, including carbohydrate and lipid metabolism. Our findings imply that RBMS1 plays an important role in adipocyte metabolism and may offer novel therapeutic opportunity for metabolic disorders such as obesity and type 2 diabetes.

Keywords

Adipogenesis; RBMS1; RNA-binding proteins; Lipid metabolism

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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