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Exploring the Potential of the Model Cyanobacteria Synecho-Coccus PCC 7002 and PCC 7942 for the Photoproduction of High-Value Terpenes: Comparison With Synechocystis PCC 6803
Chenebault, C.; Blanc-Garin, V.; Vincent, M.; Diaz-Santos, E.; Goudet, A.; Cassier-Chauvat, C.; Chauvat, F. Exploring the Potential of the Model Cyanobacteria Synechococcus PCC 7002 and PCC 7942 for the Photoproduction of High-Value Terpenes: A Comparison with Synechocystis PCC 6803. Biomolecules2023, 13, 504.
Chenebault, C.; Blanc-Garin, V.; Vincent, M.; Diaz-Santos, E.; Goudet, A.; Cassier-Chauvat, C.; Chauvat, F. Exploring the Potential of the Model Cyanobacteria Synechococcus PCC 7002 and PCC 7942 for the Photoproduction of High-Value Terpenes: A Comparison with Synechocystis PCC 6803. Biomolecules 2023, 13, 504.
Chenebault, C.; Blanc-Garin, V.; Vincent, M.; Diaz-Santos, E.; Goudet, A.; Cassier-Chauvat, C.; Chauvat, F. Exploring the Potential of the Model Cyanobacteria Synechococcus PCC 7002 and PCC 7942 for the Photoproduction of High-Value Terpenes: A Comparison with Synechocystis PCC 6803. Biomolecules2023, 13, 504.
Chenebault, C.; Blanc-Garin, V.; Vincent, M.; Diaz-Santos, E.; Goudet, A.; Cassier-Chauvat, C.; Chauvat, F. Exploring the Potential of the Model Cyanobacteria Synechococcus PCC 7002 and PCC 7942 for the Photoproduction of High-Value Terpenes: A Comparison with Synechocystis PCC 6803. Biomolecules 2023, 13, 504.
Abstract
We have performed the first comparative analysis of the potential of two physiologically-diverse model cyanobacteria, Synechococcus PCC 7002 (S.7002) and Synechococcus PCC 7942 (S.7942), for the photosynthetic production of four chemically-different high-value terpenes: two monoterpenes limonene and pinene, and two sesquiterpenes bisabolene and farnesene. We showed, for the first time, that S.7002 and S.7942 can produce farnesene and bisabolene, respectively. Both cyanobac-teria produced farnesene (S.7942 better than S.7002) more efficiently than the other tested terpenes (especially pinene the weakest produced terpene). S.7002 produced limonene better than bisabo-lene, whereas S.7942 produced bisabolene better than limonene. These findings suggest that S.7942 is better suited to produce sesquiterpenes than monoterpenes. Also interestingly, higher levels of terpenes were produced by S.7942 and S.7002 expressing a terpene-synthase gene from both a RSF1010-derived replicating plasmid and a neutral chromosomal site, as compared to ei-ther the plasmid alone or the chromosome alone. These results suggest that in both cyanobacteria the production of terpenes is more limited by the activity of terpene synthases than the abondance of terpene precursors. Finally, higher levels of terpenes were produced by S.7002 growing on urea (a frequent pollutant) as compared to nitrate (or ammonium) the standard nitrogen sources for cyanobacteria (more expensive than urea).
Biology and Life Sciences, Biology and Biotechnology
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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