Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Traumatic Brain Injury Induced Microglial and Caspase3 Activation in the Retina

Tamás Kovács-Öller
* ORCID logo ,
Renáta Zempléni
,
Boglárka Balogh
,
Gergely Szarka
,
Bálint Fazekas
,
Ádám J Tengölics
,
Krisztina Amrein
,
Endre Czeiter
,
István Hernádi
ORCID logo ,
András Büki
,
Béla Völgyi
ORCID logo
Version 1 : Received: 31 December 2022 / Approved: 3 January 2023 / Online: 3 January 2023 (08:38:56 CET)

A peer-reviewed article of this Preprint also exists.

Kovács-Öller, T.; Zempléni, R.; Balogh, B.; Szarka, G.; Fazekas, B.; Tengölics, Á.J.; Amrein, K.; Czeiter, E.; Hernádi, I.; Büki, A.; Völgyi, B. Traumatic Brain Injury Induces Microglial and Caspase3 Activation in the Retina. Int. J. Mol. Sci. 2023, 24, 4451. Kovács-Öller, T.; Zempléni, R.; Balogh, B.; Szarka, G.; Fazekas, B.; Tengölics, Á.J.; Amrein, K.; Czeiter, E.; Hernádi, I.; Büki, A.; Völgyi, B. Traumatic Brain Injury Induces Microglial and Caspase3 Activation in the Retina. Int. J. Mol. Sci. 2023, 24, 4451.

Abstract

A Traumatic brain injury (TBI) is among the main causes of sudden death after head trauma. These injuries can result in severe degeneration and neuronal cell death in the CNS, including the retina which is a crucial part of the brain responsible for perceiving and transmitting visual information. The long-term effects of mild-repetitive TBI (rmTBI) are far less studied thus far, even though damages induced by repetitive injuries occurring in the brain are more common, especially amongst athletes. rmTBI can also have a detrimental effect on the retina and the pathophysiology of these injuries are likely to differ from the severe TBI (sTBI) retinal injury.Here we showed how rmTBI and sTBI can dissimilarly affect the retina. Our results indicate an increase in the number of activated microglial cells and Caspase3-positive cells in the retina in both traumatic models, suggesting a rise in the level of inflammation and cell death after TBI. The pattern of microglial activation appears evenly distributed and widespread but differs amongst the various retinal layers. sTBI induced microgial activation in both the superficial and deep retinal layers. In contrast to sTBI, no significant change occurred following the repetitive mild injury in the superficial layer, only the deep layer (spanning from the inner nuclear layer to the outer plexiform layer) shows microglial activation. This difference suggests that alternate response mechanisms play a role in the case of the different TBI incidents. The Caspase3 activation pattern showed a uniform increase in both the superficial and deep layers of the retina. This suggests a different action in the course of the disease in sTBI and rmTBI models and points to the need for new diagnostic procedures.Our present results suggest that the retina might serve as such a model of head injuries since the retinal tissue reacts to both forms of TBI and is the most accessible part of the human brain.

Keywords

TBI; brain; injury; microglia; caspase; apoptosis; retina; degeneration

Subject

Biology and Life Sciences, Aging

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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