PreprintArticleVersion 2Preserved in Portico This version is not peer-reviewed
The Composition of Adipose-Derived Regenerative Cells Isolated from Lipoaspirate using a Novel Point of Care System does not Depend on the Subject's Individual Age, Gender, Body Mass Index and Ethnicity
Version 1
: Received: 20 September 2022 / Approved: 21 September 2022 / Online: 21 September 2022 (10:08:34 CEST)
Version 2
: Received: 4 November 2022 / Approved: 7 November 2022 / Online: 7 November 2022 (12:12:12 CET)
How to cite:
Schmitz, C.; Alt, C.; Azares, A.R.; Peace, D.A.; Facile, T.R.; Furia, J.P.; Maffulli, N.; Huang, C.; Alt, E.U. The Composition of Adipose-Derived Regenerative Cells Isolated from Lipoaspirate using a Novel Point of Care System does not Depend on the Subject's Individual Age, Gender, Body Mass Index and Ethnicity. Preprints2022, 2022090321. https://doi.org/10.20944/preprints202209.0321.v2
Schmitz, C.; Alt, C.; Azares, A.R.; Peace, D.A.; Facile, T.R.; Furia, J.P.; Maffulli, N.; Huang, C.; Alt, E.U. The Composition of Adipose-Derived Regenerative Cells Isolated from Lipoaspirate using a Novel Point of Care System does not Depend on the Subject's Individual Age, Gender, Body Mass Index and Ethnicity. Preprints 2022, 2022090321. https://doi.org/10.20944/preprints202209.0321.v2
Schmitz, C.; Alt, C.; Azares, A.R.; Peace, D.A.; Facile, T.R.; Furia, J.P.; Maffulli, N.; Huang, C.; Alt, E.U. The Composition of Adipose-Derived Regenerative Cells Isolated from Lipoaspirate using a Novel Point of Care System does not Depend on the Subject's Individual Age, Gender, Body Mass Index and Ethnicity. Preprints2022, 2022090321. https://doi.org/10.20944/preprints202209.0321.v2
APA Style
Schmitz, C., Alt, C., Azares, A.R., Peace, D.A., Facile, T.R., Furia, J.P., Maffulli, N., Huang, C., & Alt, E.U. (2022). The Composition of Adipose-Derived Regenerative Cells Isolated from Lipoaspirate using a Novel Point of Care System does not Depend on the Subject's Individual Age, Gender, Body Mass Index and Ethnicity. Preprints. https://doi.org/10.20944/preprints202209.0321.v2
Chicago/Turabian Style
Schmitz, C., Claire Huang and Eckhard U. Alt. 2022 "The Composition of Adipose-Derived Regenerative Cells Isolated from Lipoaspirate using a Novel Point of Care System does not Depend on the Subject's Individual Age, Gender, Body Mass Index and Ethnicity" Preprints. https://doi.org/10.20944/preprints202209.0321.v2
Abstract
Uncultured, unmodified, autologous, adipose-derived regenerative cells (UA-ADRCs) are a safe and effective treatment option for various musculoskeletal pathologies. However, it is unknown whether the composition of the final cell suspension systematically varies with the subject's individual age, sex, body mass index and ethnicity. UA-ADRCs were isolated from lipoaspirate from n=232 subjects undergoing elective lipoplasty using the Transpose RT system (InGeneron, Inc.; Houston, TX, USA). The UA-ADRCs were assessed for the number of nucleated cells, cell viability and the number of viable nucleated cells per gram of adipose tissue harvested. Cells from n=37 subjects were further characterized using four-channel flow cytometry. The present study shows, for the first time, that key characteristics of UA-ADRCs can be independent of the subject's age, sex, BMI and ethnicity. This result has important implications for the general applicability of UA-ADRCs in regeneration of musculoskeletal tissue. Future studies must determine whether in-dependence of key characteristics of UA-ADRCs of the subject's individual age, sex, BMI and eth-nicity only applies to the system used in the present study, or also to others of the more than 25 different experimental methods and commercially available systems used to isolate UA-ADRCs from lipoaspirate that have been described in the literature.
Medicine and Pharmacology, Medicine and Pharmacology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Commenter: Eckhard Alt
Commenter's Conflict of Interests: Author