Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

MISF2 Encodes an Essential Mitochondrial Splicing Co-factor Required for nad2 mRNA Processing and Embryo Development in Arabidopsis thaliana

Tan-Trung Nguyen
ORCID logo ,
Corinne Best
,
Sofia Shevtsov
,
Michal Zmudjak
,
Martine Quadrado
,
Ron Mizrahi
,
Hagit Zer
,
Hakim Mireau
* ORCID logo ,
Oren Ostersetzer-Biran
* ORCID logo
Version 1 : Received: 5 January 2022 / Approved: 10 January 2022 / Online: 10 January 2022 (12:46:06 CET)

A peer-reviewed article of this Preprint also exists.

Nguyen, T.-T.; Best, C.; Shevtsov, S.; Zmudjak, M.; Quadrado, M.; Mizrahi, R.; Zer, H.; Mireau, H.; Ostersetzer-Biran, O. MISF2 Encodes an Essential Mitochondrial Splicing Cofactor Required for nad2 mRNA Processing and Embryo Development in Arabidopsis thaliana. Int. J. Mol. Sci. 2022, 23, 2670. Nguyen, T.-T.; Best, C.; Shevtsov, S.; Zmudjak, M.; Quadrado, M.; Mizrahi, R.; Zer, H.; Mireau, H.; Ostersetzer-Biran, O. MISF2 Encodes an Essential Mitochondrial Splicing Cofactor Required for nad2 mRNA Processing and Embryo Development in Arabidopsis thaliana. Int. J. Mol. Sci. 2022, 23, 2670.

Abstract

Mitochondria play key roles in cellular energy metabolism in eukaryotes. Mitochondria of most organisms contain their own genome and specific transcription and translation machineries. The expression of angiosperm mtDNA involves extensive RNA-processing steps, such as RNA trimming, editing, and the splicing of numerous group II-type introns. Pentatricopeptide repeat (PPR) proteins are key players of plant organelle gene expression and RNA metabolism. In the present analysis, we reveal the function of the MITOCHONDRIAL SPLICING FACTOR 2 gene (MISF2, AT3G22670) and show that it encodes a mitochondria-localized PPR protein that is crucial for early embryo-development in Arabidopsis. Molecular characterization of embryo-rescued misf2 plantlets indicates that the splicing of nad2 intron 1 and thus respiratory complex I biogenesis are strongly compromised. Moreover, the molecular function seems conserved between MISF2 protein in Arabidopsis and its orthologous gene (EMP10) in maize, suggesting that the ancestor of MISF2/EMP10 was recruited to function in nad2 processing before the monocot-dicot divergence, ~200 million years ago. These data provide new insights into the function of nuclear-encoded factors in mitochondrial gene expression and respiratory chain biogenesis during plant embryo development.

Keywords

Group II; Intron; Splicing; PPR; Respiration; Complex I; Mitochondria; Embryogenesis; Arabidopsis; Angiosperms.

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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