Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Full Length Transcriptome Highlights the Coordination of Plastid Transcript Processing

Marine Guilcher
,
Arnaud Liehrmann
,
Chloé Seyman
ORCID logo ,
Thomas Blein
ORCID logo ,
Guillem Rigaill
,
Benoit Castandet
,
Etienne Delannoy
* ORCID logo
Version 1 : Received: 29 August 2021 / Approved: 31 August 2021 / Online: 31 August 2021 (11:48:48 CEST)

A peer-reviewed article of this Preprint also exists.

Guilcher, M.; Liehrmann, A.; Seyman, C.; Blein, T.; Rigaill, G.; Castandet, B.; Delannoy, E. Full Length Transcriptome Highlights the Coordination of Plastid Transcript Processing. Int. J. Mol. Sci. 2021, 22, 11297. Guilcher, M.; Liehrmann, A.; Seyman, C.; Blein, T.; Rigaill, G.; Castandet, B.; Delannoy, E. Full Length Transcriptome Highlights the Coordination of Plastid Transcript Processing. Int. J. Mol. Sci. 2021, 22, 11297.

Abstract

Plastid gene expression involves many post-transcriptional maturation steps resulting in a complex transcriptome composed of multiple isoforms. Although short read RNA-seq has considerably improved our understanding of the molecular mechanisms controlling these processes, it is unable to sequence full-length transcripts. This information is however crucial when it comes to understand the interplay between the various steps of plastid gene expression. Here, the study of the Arabidopsis leaf plastid transcriptome using Nanopore sequencing showed that many splicing and editing events were not independent but co-occurring. For a given transcript, maturation events also appeared to be chronologically ordered with splicing happening after most sites are edited.

Keywords

Arabidopsis thaliana; plastid; co-maturation; post-transcriptional; Nanopore

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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