Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

AuNP Coupled Rapid Flow-Through Dot-Blot Immuno-Assay for COVID-19 (SARS-CoV-2) Explicit IgG Antibody Revelation

Bijon Kumar Sil
,
Mohd. Raeed Jamiruddin
ORCID logo ,
Md. Ahsanul Haq
,
Mohib Ullah Khondoker
,
Nowshin Jahan
,
Shahad Saif Khandker
,
Tamanna Ali
,
Mumtarin Jannat Oishee
,
Taku Kaitsuka
ORCID logo ,
Masayasu Mie
ORCID logo ,
Kazuhito Tomizawa
ORCID logo ,
Eiry Kobatake
,
Mainul Haque
* ,
Nihad Adnan
* ORCID logo
Version 1 : Received: 18 April 2021 / Approved: 20 April 2021 / Online: 20 April 2021 (11:36:09 CEST)

How to cite: Sil, B.K.; Jamiruddin, M.R.; Haq, M.A.; Khondoker, M.U.; Jahan, N.; Khandker, S.S.; Ali, T.; Oishee, M.J.; Kaitsuka, T.; Mie, M.; Tomizawa, K.; Kobatake, E.; Haque, M.; Adnan, N. AuNP Coupled Rapid Flow-Through Dot-Blot Immuno-Assay for COVID-19 (SARS-CoV-2) Explicit IgG Antibody Revelation. Preprints 2021, 2021040534. https://doi.org/10.20944/preprints202104.0534.v1 Sil, B.K.; Jamiruddin, M.R.; Haq, M.A.; Khondoker, M.U.; Jahan, N.; Khandker, S.S.; Ali, T.; Oishee, M.J.; Kaitsuka, T.; Mie, M.; Tomizawa, K.; Kobatake, E.; Haque, M.; Adnan, N. AuNP Coupled Rapid Flow-Through Dot-Blot Immuno-Assay for COVID-19 (SARS-CoV-2) Explicit IgG Antibody Revelation. Preprints 2021, 2021040534. https://doi.org/10.20944/preprints202104.0534.v1

Abstract

Background: Flow-through dot-blot assay (FT-DBA) for SARS-CoV-2 specific IgG detection will provide a reliable and affordable immunoassay for the rapid serosurveillance against COVID-19. Method: SARS-CoV-2 antigens were immobilized on nitrocellulose membrane to capture IgG immunoglobulins, which were then detected with AuNP anti-human IgG. A total of 181 samples were characterized with in-house and commercial immunoassay. The positive panel consisted of RT-PCR positive samples from patients with both <14 days and >14 days from the onset of symptoms, while the negative panel contained samples collected either from the pre-pandemic era dengue patients from healthy donors during the pandemic period. Results: In-house ELISA selected a total of 79 true seropositive and 100 seronegative samples. The sensitivity of samples with <14 days using FT-DBA was 94.7% which increased to 100% for samples >14 days. The overall detection sensitivity and specificity were 98.8% and 98%, respectively, whereas the overall PPV and NPV were 97.6% and 99%. Moreover, comparative analysis between ELISA and FT-DBA revealed clinical agreement of Cohen’s Kappa value of 0.944. Conclusion: The assay can confirm past SARS-CoV-2 infection with high accuracy within 2 minutes compared to ELISA. It can help track SARS-CoV-2 disease progression, population screening, and vaccination response.

Keywords

COVID-19, SARS-CoV-2, Serological test, Sensitivity, Specificity, Serosurveillance.

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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