Version 1
: Received: 20 November 2020 / Approved: 23 November 2020 / Online: 23 November 2020 (14:23:47 CET)
How to cite:
Go, R.; YONG, C.S.; Abdullah, J.O.; Yoh, K.H. Phylogenetic of the Orchid Genus Coelogyne Inferred from Morphological Characteristics and Internal Transcribed Spacer (ITS) Sequence Data. Preprints2020, 2020110589. https://doi.org/10.20944/preprints202011.0589.v1
Go, R.; YONG, C.S.; Abdullah, J.O.; Yoh, K.H. Phylogenetic of the Orchid Genus Coelogyne Inferred from Morphological Characteristics and Internal Transcribed Spacer (ITS) Sequence Data. Preprints 2020, 2020110589. https://doi.org/10.20944/preprints202011.0589.v1
Go, R.; YONG, C.S.; Abdullah, J.O.; Yoh, K.H. Phylogenetic of the Orchid Genus Coelogyne Inferred from Morphological Characteristics and Internal Transcribed Spacer (ITS) Sequence Data. Preprints2020, 2020110589. https://doi.org/10.20944/preprints202011.0589.v1
APA Style
Go, R., YONG, C.S., Abdullah, J.O., & Yoh, K.H. (2020). Phylogenetic of the Orchid Genus Coelogyne Inferred from Morphological Characteristics and Internal Transcribed Spacer (ITS) Sequence Data. Preprints. https://doi.org/10.20944/preprints202011.0589.v1
Chicago/Turabian Style
Go, R., Janna Ong Abdullah and Kok Hon Yoh. 2020 "Phylogenetic of the Orchid Genus Coelogyne Inferred from Morphological Characteristics and Internal Transcribed Spacer (ITS) Sequence Data" Preprints. https://doi.org/10.20944/preprints202011.0589.v1
Abstract
The phylogenetic relationships among the Peninsular Malaysian orchid genus Coelogyne were studied by morphological characteristics and by sequencing the internal transcribed region (ITS) from the nuclear ribosomal DNA (nrDNA). Coelogyne is a large genus of about 200 species distributed in pantropical areas from the Himalayas, Sri Lanka, India, Southern China and throughout South East Asia to Papua New Guinea. The widely accepted previous classification system was exclusively based on floral morphology. There were very few molecular systematic studies of Coelogyne done in Peninsular Malaysia thus far. In this study, 59 Coelogyne taxa were collected from throughout Peninsular Malaysia and 57 of them were identified to the species level. To study the phylogeny of this genus, morphological characters were utilized together with molecular evidences to generate the systematic hypotheses. Cluster analysis was performed using both the vegetative and floral characters. The results showed that three sections of Peninsular Malaysian Coelogyne, namely Longifoliae, Speciosae and Fuliginosae were sister groups which were more closely related by forming one clade than they were with the other sections. Another clade consisted of four other sections, namely Flaccidae, Coelogynae, Tomentosae and Verrucosae. Molecular phylogenies obtained by using the Neighbour Joining method revealed the close relationship between the sections Tomentosae and Verrucosae, whereas usage of the Maximum Likelihood method demonstrated that three sections namely Longifoliae, Speciosae and Fuliginosae were sister groups since they formed a single clade.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
