Targeted-Protein Silencing Tools: Overview and Future Perspectives

Yuri Prozzillo; Gaia Fattorini; Maria Virginia Santopietro; Luigi Suglia; Alessandra Ruggiero; Diego Ferreri; Giovanni Messina

doi:10.20944/preprints202011.0061.v1

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preprints.org > biology and life sciences > anatomy and physiology > doi: 10.20944/preprints202011.0061.v1

Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

Targeted-Protein Silencing Tools: Overview and Future Perspectives

Yuri Prozzillo

^* ,

Gaia Fattorini

Maria Virginia Santopietro

Version 1 : Received: 30 October 2020 / Approved: 2 November 2020 / Online: 2 November 2020 (15:59:46 CET)

A peer-reviewed article of this Preprint also exists.

Prozzillo, Y.; Fattorini, G.; Santopietro, M.V.; Suglia, L.; Ruggiero, A.; Ferreri, D.; Messina, G. Targeted Protein Degradation Tools: Overview and Future Perspectives. Biology 2020, 9, 421. Prozzillo, Y.; Fattorini, G.; Santopietro, M.V.; Suglia, L.; Ruggiero, A.; Ferreri, D.; Messina, G. Targeted Protein Degradation Tools: Overview and Future Perspectives. Biology 2020, 9, 421.

Abstract

Targeted Protein Silencing (TPS) is an elegant approach to investigate protein function and its role in the cellular landscape, overcoming limitations of genetic perturbation strategies. In contrast to CRISPR/Cas9 and RNA interference, these systems act in a reversible manner and reduce off-target effects. Several TPS have been developed and wisely improved, including compartment delocalization tools and protein degradation systems. In this review, we focus on Anchor-Away, deGradFP, auxin inducible degron (AID) and dTAG technologies, and discuss their recent applications and advances. Finally, we propose Nano-Grad, a novel nanobody-based protein degradation tool to specifically proteolyze endogenous tag-free target protein.

Keywords

Targeted Protein Silencing (TPS); Targeted Protein Degradation (TPD); dTAG; FKBP12; von Hippel-Lindau (VHL); degron; deGradFP; Anchor-Away; Nanobody; Nano-Grad

Subject

Biology and Life Sciences, Anatomy and Physiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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