Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Simple, Low-cost and Semi-permanent Film for Virus Inactivation Using Avian Coronavirus Model as Challenge

Luiz Alberto Colnago
* ORCID logo ,
Iara Maria Trevisol
ORCID logo ,
Daiane Voss Rech
ORCID logo ,
Lucimara Aparecida Forato
,
Cirlei Igreja do Nascimento Mitre
,
José Paulo Gagliardi Leite
,
Cintia Hiromi Okino
*
Version 1 : Received: 16 August 2020 / Approved: 19 August 2020 / Online: 19 August 2020 (10:45:07 CEST)

A peer-reviewed article of this Preprint also exists.

Colnago, L.A.; Trevisol, I.M.; Voss Rech, D.; Forato, L.A.; Igreja do Nascimento Mitre, C.; Gagliardi Leite, J.P.; Giglioti, R.; Okino, C.H. Simple, Low-Cost and Long-Lasting Film for Virus Inactivation Using Avian Coronavirus Model as Challenge. Int. J. Environ. Res. Public Health 2020, 17, 6456. Colnago, L.A.; Trevisol, I.M.; Voss Rech, D.; Forato, L.A.; Igreja do Nascimento Mitre, C.; Gagliardi Leite, J.P.; Giglioti, R.; Okino, C.H. Simple, Low-Cost and Long-Lasting Film for Virus Inactivation Using Avian Coronavirus Model as Challenge. Int. J. Environ. Res. Public Health 2020, 17, 6456.

Abstract

COVID-19 infection, caused by SARS-CoV-2, is inequitably distributed and more lethal among populations with lower socioeconomic status. Direct contact with contaminated surfaces has been one of the virus sources, as it remains infective up to days. Several disinfectants have been shown to inactivate SARS-CoV-2 but they rapidly evaporate, are flammable or toxic and may be scarce or inexistent for the vulnerable populations. Therefore, we are proposing a simple, easy to prepare, low-cost and efficient antiviral films, made with wide available dishwasher detergent, which can be spread in hands and inanimate surfaces and maintains virucidal activity for longer periods than the current sanitizers. Avian coronavirus (ACoV) was used as model of challenge to test the antivirus efficacy of proposed films. Polystyrene microplates were covered with a thin layer of detergent formula. After drying, the films were exposed to different virus doses for 10 minutes and virus infectivity were determined using embryonated chicken eggs and RNA virus quantification in allantoic fluids by RT-qPCR. The films showed to inactive the ACoV (ranging from 103.66 to 106.66 EID50), which is chemically and morphologically similar to SARSCoV-2 and may constitute an excellent alternative to minimize the spread of Covid-19.

Keywords

coronavirus; film; detergent; antiviral; virucide; inactivation; sanitization

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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