Astuti, I.; Sofyannoor, G.M.; Oktriani, R.; Wardana, T.; Anwar, S.L.; Aryandono, T.; Mubarika Haryana, S. MicroRNA-21 and PTEN Expression Levels Are Negatively Correlated in Doxorubicin Resistant MCF-7 Breast Cancer Cell Line. Preprints2019, 2019030287. https://doi.org/10.20944/preprints201903.0287.v1
APA Style
Astuti, I., Sofyannoor, G.M., Oktriani, R., Wardana, T., Anwar, S.L., Aryandono, T., & Mubarika Haryana, S. (2019). MicroRNA-21 and PTEN Expression Levels Are Negatively Correlated in Doxorubicin Resistant MCF-7 Breast Cancer Cell Line. Preprints. https://doi.org/10.20944/preprints201903.0287.v1
Chicago/Turabian Style
Astuti, I., Teguh Aryandono and Sofia Mubarika Haryana. 2019 "MicroRNA-21 and PTEN Expression Levels Are Negatively Correlated in Doxorubicin Resistant MCF-7 Breast Cancer Cell Line" Preprints. https://doi.org/10.20944/preprints201903.0287.v1
Abstract
Background: Breast cancer is the leading cause of cancer mortality and morbidity among Indonesian women. Identification of biological pathways leading into therapeutic resistance through in vitro model is an important step to develop alternative effective therapy in breast cancer. Loss of PTEN expression has been associated with resistance to chemotherapy by involving PI3K/PTEN- dependent apoptosis pathway. We conducted in vitro experiment to investigate the association of hsa-miR-21 and PTEN expression in Doxorubicin-resistant MCF-7 cell line. Methods: Parental MCF-7 cells were periodically incubated with Doxorubicin to obtain specific Dox-resistant variant determined by IC50 using MTT assay. PTEN protein expression was analyzed using immunocytochemistry. Expression of mature has-miR-21 was measured using qRT-PCR. Results: The IC50 of Doxorubicin in parental MCF-7 and Doxorubicin-resistant MCF-7 cells (MCF-7/Dox) was 0.68 and 5.78 µg/ml, respectively. Hsa-miR-21 was significantly overexpressed in MCF-7/Dox cells compared to parental MCF cells (7.94 fold changes). Conclusion: PTEN and hsa-miR-21 expression levels were negatively correlated in Doxorubicin resistant-MCF cells. Further study to confirm the causal relationship of miR-21 overexpression and PTEN downregulation in MCF-7/Dox is required.
Keywords
resistant; microRNA-21; doxorubicin; PTEN; MCF-7/Dox cell line
Subject
Medicine and Pharmacology, Oncology and Oncogenics
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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