Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Differential Methylation in APOE Genes (Chr19; exon four; from 44,909,188 to 44,909,373) and Increased Apolipoprotein E Plasmatic Levels in Subjects with Mild Cognitive Impairment”

Oscar Mancera Páez
* ORCID logo ,
Kelly Estrada Orozco
ORCID logo ,
Maria Fernanda Mahecha
,
Francy Cruz Sanabria
,
Kely Bonilla-Vargas
,
Nicolas Sandoval
,
Esneyder Guerrero
ORCID logo ,
David Salcedo-Tacuma
,
Jesús Melgarejo
,
Edwin Vega
,
Jenny Ortega
,
Gustavo C. Roman
* ,
Rodrigo Pardo-Turriago
* ,
Humberto Arboleda
* ORCID logo
Version 1 : Received: 30 January 2019 / Approved: 1 February 2019 / Online: 1 February 2019 (09:22:48 CET)

A peer-reviewed article of this Preprint also exists.

Mancera-Páez, O.; Estrada-Orozco, K.; Mahecha, M.F.; Cruz, F.; Bonilla-Vargas, K.; Sandoval, N.; Guerrero, E.; Salcedo-Tacuma, D.; Melgarejo, J.D.; Vega, E.; Ortega-Rojas, J.; Román, G.C.; Pardo-Turriago, R.; Arboleda, H. Differential Methylation in APOE (Chr19; Exon Four; from 44,909,188 to 44,909,373/hg38) and Increased Apolipoprotein E Plasma Levels in Subjects with Mild Cognitive Impairment. Int. J. Mol. Sci. 2019, 20, 1394. Mancera-Páez, O.; Estrada-Orozco, K.; Mahecha, M.F.; Cruz, F.; Bonilla-Vargas, K.; Sandoval, N.; Guerrero, E.; Salcedo-Tacuma, D.; Melgarejo, J.D.; Vega, E.; Ortega-Rojas, J.; Román, G.C.; Pardo-Turriago, R.; Arboleda, H. Differential Methylation in APOE (Chr19; Exon Four; from 44,909,188 to 44,909,373/hg38) and Increased Apolipoprotein E Plasma Levels in Subjects with Mild Cognitive Impairment. Int. J. Mol. Sci. 2019, 20, 1394.

Abstract

Background: Biomarkers are essential for identification of individuals at high risk of mild cognitive impairment (MCI) for potential prevention of dementia. We investigated DNA methylation in the ApoE gene and plasmatic apolipoprotein E (ApoE) levels as MCI biomarkers in Colombian subjects with MCI and controls. Methods: 100 participants were included (71% women, average age, 70 yrs., range 43-91). MCI was diagnosed by neuropsychological testing, medical and social history, activities of daily living, cognitive symptoms and neuroimaging. Multivariate logistic regression models adjusted by age and gender were performed to examine the risk association of MCI with plasma ApoE and APOE methylation Results: MCI was diagnosed in 41 subjects (average age, 66.5±9.6 yrs.) and compared with 59 controls. Elevated plasma ApoE and APOE methylation of CpGs 165, 190, and 198 were risk factors for MCI (P<0.05). Higher CpG-227 methylation correlated with lower risk for MCI (P=0.002). Only CpG-227 was significantly correlated with plasmatic ApoE levels (correlation coefficient=-0.665; P=0.008). Conclusion: Differential APOE methylation and increased plasma ApoE levels were correlated with MCI. These epigenetic patterns can be used as potential biomarkers to identify early stages of MCI.

Keywords

APOE gene; Apolipoprotein E; DNA methylation; Mild cognitive impairment; Hispanics.

Subject

Medicine and Pharmacology, Neuroscience and Neurology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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