Version 1
: Received: 5 September 2018 / Approved: 5 September 2018 / Online: 5 September 2018 (04:49:40 CEST)
How to cite:
Evsikov, A.; Marín de Evsikova, C. Transcriptomics as Precision Medicine to Classify In Vivo Models of Dietary-Induced Atherosclerosis at Cellular and Molecular Levels. Preprints2018, 2018090082. https://doi.org/10.20944/preprints201809.0082.v1
Evsikov, A.; Marín de Evsikova, C. Transcriptomics as Precision Medicine to Classify In Vivo Models of Dietary-Induced Atherosclerosis at Cellular and Molecular Levels. Preprints 2018, 2018090082. https://doi.org/10.20944/preprints201809.0082.v1
Evsikov, A.; Marín de Evsikova, C. Transcriptomics as Precision Medicine to Classify In Vivo Models of Dietary-Induced Atherosclerosis at Cellular and Molecular Levels. Preprints2018, 2018090082. https://doi.org/10.20944/preprints201809.0082.v1
APA Style
Evsikov, A., & Marín de Evsikova, C. (2018). Transcriptomics as Precision Medicine to Classify <em>In Vivo</em> Models of Dietary-Induced Atherosclerosis at Cellular and Molecular Levels. Preprints. https://doi.org/10.20944/preprints201809.0082.v1
Chicago/Turabian Style
Evsikov, A. and Caralina Marín de Evsikova. 2018 "Transcriptomics as Precision Medicine to Classify <em>In Vivo</em> Models of Dietary-Induced Atherosclerosis at Cellular and Molecular Levels" Preprints. https://doi.org/10.20944/preprints201809.0082.v1
Abstract
The central promise of personalized medicine is individualized treatments that target molecular mechanisms underlying the physiological changes and symptoms arising from disease. We demonstrate a bioinformatics analysis pipeline as a proof-of-principle to test the feasibility and practicality of comparative transcriptomics to classify two of the most popular in vivo diet-induced models of coronary atherosclerosis, apolipoprotein E null mice and New Zealand White rabbits. Transcriptomics analyses indicate the two models extensively share dysregulated genes albeit with some unique pathways. For instance, while both models have alterations in the mitochondrion, the biochemical pathway analysis revealed, Complex IV in the electron transfer chain is higher in mice, whereas the rest of the electron transfer chain components are higher in the rabbits. Several fatty acids anabolic pathways are expressed higher in mice, whereas fatty acids and lipids degradation pathways are higher in rabbits. This reflects the differences between two translational models of atherosclerosis. This study validates transcriptome analysis as a potential method to precisely identify altered cellular and molecular pathways in atherosclerotic disease, which can be used to individualize treatment even in the absence of genetic data.
Keywords
atherosclerosis; coronary aortic disease; gene set enrichment analysis; heart disease; Apoe mouse; transcriptomics; RNA-seq analysis; pathway enrichment analysis; mouse; precision medicine; New Zealand White rabbit
Subject
Medicine and Pharmacology, Cardiac and Cardiovascular Systems
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.