Version 1
: Received: 24 March 2018 / Approved: 27 March 2018 / Online: 27 March 2018 (05:22:15 CEST)
How to cite:
Dang, Y.; Waxman, S.; Wang, C.; Loewen, R.T.; Sun, M.; Loewen, N.A. A Porcine Ex Vivo Model of Pigmentary Glaucoma. Preprints2018, 2018030219. https://doi.org/10.20944/preprints201803.0219.v1
Dang, Y.; Waxman, S.; Wang, C.; Loewen, R.T.; Sun, M.; Loewen, N.A. A Porcine Ex Vivo Model of Pigmentary Glaucoma. Preprints 2018, 2018030219. https://doi.org/10.20944/preprints201803.0219.v1
Dang, Y.; Waxman, S.; Wang, C.; Loewen, R.T.; Sun, M.; Loewen, N.A. A Porcine Ex Vivo Model of Pigmentary Glaucoma. Preprints2018, 2018030219. https://doi.org/10.20944/preprints201803.0219.v1
APA Style
Dang, Y., Waxman, S., Wang, C., Loewen, R.T., Sun, M., & Loewen, N.A. (2018). A Porcine Ex Vivo Model of Pigmentary Glaucoma. Preprints. https://doi.org/10.20944/preprints201803.0219.v1
Chicago/Turabian Style
Dang, Y., Ming Sun and Nils A. Loewen. 2018 "A Porcine Ex Vivo Model of Pigmentary Glaucoma" Preprints. https://doi.org/10.20944/preprints201803.0219.v1
Abstract
Pigment dispersion can lead to pigmentary glaucoma, a poorly understood condition of younger myopic eyes with fluctuating high intraocular pressure. It has been difficult to investigate its pathogenesis without a model similar to human eyes in size and behavior. Here we present a porcine ex vivo model that recreates several features of pigmentary glaucoma, including intraocular hypertension, accumulation of pigment in the trabecular meshwork, and declining phagocytosis. We found that trabecular meshwork cells regulate outflow, form actin stress fibers, and have a decreased phagocytic activity. Gene expression microarrays and a pathway analysis of TM monolayers as well as ex vivo anterior segment perfusion cultures indicated that RhoA plays a central role in regulating the cytoskeleton, motility, and phagocytosis in the trabecular meshwork, providing new insights and targets to investigate in pigmentary glaucoma.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
