Preprint Article Version 1 NOT YET PEER-REVIEWED

Proteomic Comparative Analysis of Pregnancy Serum Facilitating Hepatitis E Virus Replication in Hepatoma Cells

Version 1 : Received: 6 March 2017 / Approved: 6 March 2017 / Online: 6 March 2017 (04:59:30 CET)

How to cite: Li, Y.; Bi, Y.; Yu, W.; Yang, C.; Wang, J.; Long, F.; Li, Y.; Huang, F.; Hua, X. Proteomic Comparative Analysis of Pregnancy Serum Facilitating Hepatitis E Virus Replication in Hepatoma Cells. Preprints 2017, 2017030025 (doi: 10.20944/preprints201703.0025.v1). Li, Y.; Bi, Y.; Yu, W.; Yang, C.; Wang, J.; Long, F.; Li, Y.; Huang, F.; Hua, X. Proteomic Comparative Analysis of Pregnancy Serum Facilitating Hepatitis E Virus Replication in Hepatoma Cells. Preprints 2017, 2017030025 (doi: 10.20944/preprints201703.0025.v1).

Abstract

Hepatitis E virus (HEV) is a common cause of acute hepatitis worldwide, accounting for approximately 25% of deaths among pregnant women. We previously reported that pregnancy serum facilitates HEV replication in vitro. However, the differences in host cells with HEV infection induced by pregnancy serum and fetal bovine serum (FBS) are unclear. In this study, differentially expressed proteins were identified in HEV-infected hepatoma cells (HepG2) supplemented with different sera by using isobaric tags for relative and absolute quantitation. Proteomic analysis indicated that HEV infection significantly induced 1014 differentially expressed proteins in HEV-infected HepG2 cells when supplemented with FBS compared with pregnancy serum. Further validation by Western blot confirmed that filamin A, heat-shock proteins 70 and 90, Cytochrome c, and Thioredoxin were associated with HEV infection. This comparative analysis provides an important basis to further investigate HEV pathogenesis in pregnant women and HEV replication.

Subject Areas

hepatitis E virus; proteomic comparative analysis; pregnancy serum

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