Preprint Article Version 1 NOT YET PEER-REVIEWED

Salmonella Typhimurium Induces Cloacitis-Like Symptoms in Zebrafish Larvae

Version 1 : Received: 2 February 2017 / Approved: 3 February 2017 / Online: 3 February 2017 (04:18:59 CET)

How to cite: Varas, M.; Ortíz-Severín, J.; Marcoleta, A.; Díaz-Pascual, F.; Allende, M.; Santiviago, C.; Chávez, F. Salmonella Typhimurium Induces Cloacitis-Like Symptoms in Zebrafish Larvae . Preprints 2017, 2017020006 (doi: 10.20944/preprints201702.0006.v1). Varas, M.; Ortíz-Severín, J.; Marcoleta, A.; Díaz-Pascual, F.; Allende, M.; Santiviago, C.; Chávez, F. Salmonella Typhimurium Induces Cloacitis-Like Symptoms in Zebrafish Larvae . Preprints 2017, 2017020006 (doi: 10.20944/preprints201702.0006.v1).

Abstract

Pathogenic Salmonella strains have a set of virulence factors allowing them to generate systemic infections and damage in a variety of hosts. Among these factors, bacterial proteins secreted by specialized systems are used to penetrate the host’s intestinal mucosa, through the invasion and destruction of specialized epithelial M cells in the intestine. On the other hand, numerous studies have demonstrated that humans, as well as experimental animal hosts, respond to Salmonella infection by activating both innate and adaptive immune responses. Here, through live cell imaging of S. Typhimurium infection of zebrafish larvae, we showed that besides the intestinal colonization, a deformed cloacae region and a concomitant accumulation of S. Typhimurium cells was observed upon bacterial infection. The swelling led to a persistent inflammation of infected larvae, although the infection was non-lethal. The in vivo inflammation process was confirmed by the co-localization of GFP-tagged S. Typhimurium with mCherry-tagged neutrophils at 72 hours post exposition. Our live-cell analyses suggest that Salmonella Typhimurium induce cloacitis-like symptoms in zebrafish larvae.  Typhimurium induce cloacitis-like symptoms in zebrafish larvae.

Subject Areas

Deformed cloacal region; Danio rerio; Live-Cell imaging; Salmonella infection; Immune response; Cloacitis

Readers' Comments and Ratings (0)

Discuss and rate this article
Views 0
Downloads 0
Comments 0
Metrics 0
Discuss and rate this article

×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.