Version 1
: Received: 31 January 2017 / Approved: 31 January 2017 / Online: 31 January 2017 (12:39:46 CET)
How to cite:
Brinn, M.; Al-Sarawi, S.F.; Lu, T.; Freeman, B.J.C.; Kumaratilake, J.; Henneberg, M. A Portable Live Cell Culture and Imaging System with Optional Umbilical Bioreactor Using a Modified Infant Incubator. Preprints2017, 2017010137. https://doi.org/10.20944/preprints201701.0137.v1
Brinn, M.; Al-Sarawi, S.F.; Lu, T.; Freeman, B.J.C.; Kumaratilake, J.; Henneberg, M. A Portable Live Cell Culture and Imaging System with Optional Umbilical Bioreactor Using a Modified Infant Incubator. Preprints 2017, 2017010137. https://doi.org/10.20944/preprints201701.0137.v1
Brinn, M.; Al-Sarawi, S.F.; Lu, T.; Freeman, B.J.C.; Kumaratilake, J.; Henneberg, M. A Portable Live Cell Culture and Imaging System with Optional Umbilical Bioreactor Using a Modified Infant Incubator. Preprints2017, 2017010137. https://doi.org/10.20944/preprints201701.0137.v1
APA Style
Brinn, M., Al-Sarawi, S.F., Lu, T., Freeman, B.J.C., Kumaratilake, J., & Henneberg, M. (2017). A Portable Live Cell Culture and Imaging System with Optional Umbilical Bioreactor Using a Modified Infant Incubator. Preprints. https://doi.org/10.20944/preprints201701.0137.v1
Chicago/Turabian Style
Brinn, M., Jaliya Kumaratilake and Maciej Henneberg. 2017 "A Portable Live Cell Culture and Imaging System with Optional Umbilical Bioreactor Using a Modified Infant Incubator" Preprints. https://doi.org/10.20944/preprints201701.0137.v1
Abstract
Here, we present a staged approach for an innovative repurposing of a portable infant humidicrib into a live cell growth, observation, and imaging system. Furthmore, humidicrib can support different variations of “umbilical” bioreactors, and can be used to conduct electrophysiology experiments and in situ immunohistochemistry. Modifications incorporate a closed loop carbon dioxide (CO2) concentration control system with umbilical CO2 and heating support for tailored bioreactors. The repurposing cost is inexpensive and allows for the continued observation and imaging of cells. This prototype unit has been used to continuously observe and image live primary neurons for up to 21 days. This demonstrates the repurposed units’ suitability for use in tissue culture based research, particularly where modifications to microscopes are required or where sensitive manipulation outside of a standard incubator is needed.
Keywords
cell culture; bioreactor; live cell imaging; tissue engineering
Subject
Biology and Life Sciences, Cell and Developmental Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
