Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Up-regulation of Lamin A/C Expression in Epstein-Barr Virus Immortalized B Cells and Burkitt Lymphoma Cell Lines of Activated B Cell Phenotype

Ferenc Bánáti
,
Anita Koroknai
,
Kálmán Szenthe
,
Tamás Tereh
,
Nóra Kovács
,
Anita Hidasi
,
Barbara Bánkuti
,
Krisztina Buzás
,
Frederic Lemnitzer
,
Zsolt Ruzsics
,
Susan Szathmary
,
Hans Wolf
,
Dániel Salamon
,
János Minárovits
* ,
Hans-Helmut Niller
Version 1 : Received: 24 November 2016 / Approved: 24 November 2016 / Online: 24 November 2016 (17:53:07 CET)

How to cite: Bánáti, F.; Koroknai, A.; Szenthe, K.; Tereh, T.; Kovács, N.; Hidasi, A.; Bánkuti, B.; Buzás, K.; Lemnitzer, F.; Ruzsics, Z.; Szathmary, S.; Wolf, H.; Salamon, D.; Minárovits, J.; Niller, H. Up-regulation of Lamin A/C Expression in Epstein-Barr Virus Immortalized B Cells and Burkitt Lymphoma Cell Lines of Activated B Cell Phenotype. Preprints 2016, 2016110125. https://doi.org/10.20944/preprints201611.0125.v1 Bánáti, F.; Koroknai, A.; Szenthe, K.; Tereh, T.; Kovács, N.; Hidasi, A.; Bánkuti, B.; Buzás, K.; Lemnitzer, F.; Ruzsics, Z.; Szathmary, S.; Wolf, H.; Salamon, D.; Minárovits, J.; Niller, H. Up-regulation of Lamin A/C Expression in Epstein-Barr Virus Immortalized B Cells and Burkitt Lymphoma Cell Lines of Activated B Cell Phenotype. Preprints 2016, 2016110125. https://doi.org/10.20944/preprints201611.0125.v1

Abstract

Lamin A, B and C, the nuclear intermediate-filament proteins, play a role in epigenetic regulation. While Lamin B is expressed in all nucleated cells studied, Lamin A/C are transcribed in most somatic cell types except mature B lymphocytes. Since Epstein-Barr virus (EBV), a human gammaherpesvirus, is associated with tumorigenic processes and is known to alter the epigenotype of its host cells, we studied the expression of the LMNA gene and its epigenetic marks in EBV-carrying human lymphoid cell lines. We observed a high lamin A/C mRNA and protein expression in EBV-immortalized lymphoblastoid cell lines (LCLs) and in group III Burkitt lymphoma (BL) lines where hypomethylated first exons were observed with activating histone marks. In most cell lines with low promoter activity a highly methylated first exon could be detected. Our data showed that methylation of the first exon of LMNA was associated with the downregulation of LMNA expression whereas euchromatic histone marks were enriched at active LMNA promoters in EBV-immortalized LCLs. These data suggest a role for viral latency products to activate LMNAp in EBV-infected latency type III B cells in vitro. Expression of lamin A/C may contribute to the establishment of activated B cell phenotype that needs further explorations.

Keywords

Lamin, EBV latency, transformation, epigenetic regulation, activated B cell

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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