Preprint Article Version 1 NOT YET PEER-REVIEWED

Evaluation of EphA2 and EphB4 as Targets for Image-Guided Colorectal Cancer Surgery

  1. Department of Radiology, Leiden University Medical Center, Leiden 2333 ZA, The Netherlands
  2. Percuros BV, Leiden 7522 NB, The Netherlands
  3. Department of Surgery, Leiden University Medical Center, Leiden 2333 ZA, The Netherlands
  4. Department of Nuclear Medicine, Leiden University Medical Center, Leiden 2333 ZA, The Netherlands
  5. Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA 92037, USA
  6. Antibodies for Research Applications BV, Gouda 2805 HT, The Netherlands
Version 1 : Received: 23 November 2016 / Approved: 24 November 2016 / Online: 24 November 2016 (11:07:23 CET)

How to cite: Stammes, M.; Prevoo, H.; Ter Horst, M.; Groot, S.; Van De Velde, C.; Chan, A.; De Geus-Oei, L.; Kuppen, P.; Vahrmeijer, A.; Pasquale, E.; Sier, C. Evaluation of EphA2 and EphB4 as Targets for Image-Guided Colorectal Cancer Surgery. Preprints 2016, 2016110121 (doi: 10.20944/preprints201611.0121.v1). Stammes, M.; Prevoo, H.; Ter Horst, M.; Groot, S.; Van De Velde, C.; Chan, A.; De Geus-Oei, L.; Kuppen, P.; Vahrmeijer, A.; Pasquale, E.; Sier, C. Evaluation of EphA2 and EphB4 as Targets for Image-Guided Colorectal Cancer Surgery. Preprints 2016, 2016110121 (doi: 10.20944/preprints201611.0121.v1).

Abstract

Targeted image-guided oncologic surgery (IGOS) relies on the recognition of cell surface-associated proteins, which should be abundantly present on the tumor cells but preferably absent on cells in surrounding healthy tissue. The transmembrane receptor tyrosine kinase EphA2, a member of the A class of the Eph receptor family, has been reported to be highly overexpressed in several tumor types including breast, lung, brain, prostate, and colon cancer, and is considered amongst the most promising cell membrane-associated tumor antigens by the NIH. Another member of the Eph receptor family belonging to the B class, EphB4, has also been found to be up-regulated in multiple cancer types. In this study, EphaA2 and EphB4 are evaluated as target for IGOS of colorectal cancer by immunohistochemistry (IHC) using a tissue microarray (TMA) consisting of 168 pairs of tumor and normal tissue. The IHC sections were scored for staining intensity and percentage of cells stained. The results show a significantly enhanced staining intensity and more widespread distribution in tumor tissue compared with adjacent normal tissue for EphA2 as well as EphB4. Based on its more consistently higher score in colorectal tumor tissue compared to normal tissue, EphB4 appears to be an especially promising candidate for IGOS of colorectal cancer.

Subject Areas

tissue microarray; immunohistochemistry; cancer imaging; tyrosine kinase receptor; normal tissue; colon cancer

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