Preprint Article Version 1 NOT YET PEER-REVIEWED

A Y-Shaped Microfluidic Device to Study the Combined Effect of Wall Shear Stress and ATP Signals on Intracellular Calcium Dynamics in Vascular Endothelial Cells

  1. Department of Biomedical Engineering, Dalian University of Technology, Dalian 116024, China
  2. Department of Anesthesiology, The First Affiliated Hospital of Dalian Medical University, Dalian 116011, China
Version 1 : Received: 22 November 2016 / Approved: 22 November 2016 / Online: 22 November 2016 (09:51:31 CET)

A peer-reviewed article of this Preprint also exists.

Chen, Z.-Z.; Gao, Z.-M.; Zeng, D.-P.; Liu, B.; Luan, Y.; Qin, K.-R. A Y-Shaped Microfluidic Device to Study the Combined Effect of Wall Shear Stress and ATP Signals on Intracellular Calcium Dynamics in Vascular Endothelial Cells. Micromachines 2016, 7, 213. Chen, Z.-Z.; Gao, Z.-M.; Zeng, D.-P.; Liu, B.; Luan, Y.; Qin, K.-R. A Y-Shaped Microfluidic Device to Study the Combined Effect of Wall Shear Stress and ATP Signals on Intracellular Calcium Dynamics in Vascular Endothelial Cells. Micromachines 2016, 7, 213.

Journal reference: Micromachines 2016, 7, 213
DOI: 10.3390/mi7110213

Abstract

The intracellular calcium dynamics in vascular endothelial cells (VECs) in response to wall shear stress (WSS) and/or adenosine triphosphate (ATP) have been commonly regarded as an important factor in regulating VEC function and behavior including proliferation, migration and apoptosis. However, the effects of time-varying ATP signals have been usually neglected in the past investigations in the field of VEC mechanobiology. In order to investigate the combined effects of WSS and dynamic ATP signals on the intracellular calcium dynamic in VECs, a Y-shaped microfluidic device, which can provide the cultured cells on the bottom of its mixing micro-channel with stimuli of WSS signal alone and different combinations of WSS and ATP signals in one single micro-channel, is proposed. Both numerical simulation and experimental studies verify the feasibility of its application. Cellular experimental results also suggest that a combination of WSS and ATP signals rather than a WSS signal alone might play a more significant role in VEC Ca2+ signal transduction induced by blood flow.

Subject Areas

Y-shaped microfluidic device; wall shear stress; adenosine triphosphate (ATP) signal; combined effect; vascular endothelial cells; calcium dynamics

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