Preprint Article Version 1 NOT YET PEER-REVIEWED

Molecularly Imprinted Quartz Crystal Microbalance Sensor (QCM) for Bilirubin Detection

  1. Department of Chemistry, Hacettepe University, 06800 Ankara, Turkey
  2. Department of Chemical Technology, Abant İzzet Baysal University, 14900 Bolu, Turkey
Version 1 : Received: 2 November 2016 / Approved: 2 November 2016 / Online: 2 November 2016 (17:34:43 CET)

A peer-reviewed article of this Preprint also exists.

Çiçek, Ç.; Yılmaz, F.; Özgür, E.; Yavuz, H.; Denizli, A. Molecularly Imprinted Quartz Crystal Microbalance Sensor (QCM) for Bilirubin Detection. Chemosensors 2016, 4, 21. Çiçek, Ç.; Yılmaz, F.; Özgür, E.; Yavuz, H.; Denizli, A. Molecularly Imprinted Quartz Crystal Microbalance Sensor (QCM) for Bilirubin Detection. Chemosensors 2016, 4, 21.

Journal reference: Chemosensors 2016, 4, 21
DOI: 10.3390/chemosensors4040021

Abstract

This study aims the preparation of QCM sensor for the detection of bilirubin in human plasma. Bilirubin imprinted poly-(2-hydroxyethyl methacrylate-N-methacryloyl-L-tryptophan methyl ester) (PHEMATrp) nanofilm (MIP) on the gold surface of QCM chip was synthesized by molecular imprinting technique. Meanwhile, the non-imprinted PHEMATrp (NIP) nanofilm was synthesized by the same experimental technique to examine the imprinting effect. Characterization of MIP and NIP nanofilms on the QCM chip surface was achieved by atomic force microscopy (AFM), ellipsometry, fourier transform infrared spectrophotometry-attenuated total reflectance (FTIR-ATR) and contact angle measurements (CA). The observations indicated that the nanofilm was almost monolayer. Thereinafter, the imprinted and the non-imprinted QCM chipss were connected to QCM system to investigate kinetic and affinity properties. In order to examine the selectivity of the MIP-PHEMATrp nanofilm, competitive adsorption of bilirubin, cholesterol and estradiol was performed. Limit of detection (LOD) and limit of quantitation (LOQ) values were calculated as 0.45 μg/mL and 0.9 μg/mL, respectively.

Subject Areas

molecular imprinting technique; bilirubin; QCM; PHEMA; nanosensor

Readers' Comments and Ratings (0)

Discuss and rate this article
Views 147
Downloads 136
Comments 0
Metrics 0
Discuss and rate this article

×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.