Preprint Article Version 1 NOT YET PEER-REVIEWED

AAC as a Potential Target Gene to Control Verticillium dahliae

  1. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing; China
Version 1 : Received: 2 November 2016 / Approved: 2 November 2016 / Online: 2 November 2016 (16:27:40 CET)

How to cite: Su, X.; Rehman, L.; Guo, H.; Li, X.; Cheng, H. AAC as a Potential Target Gene to Control Verticillium dahliae. Preprints 2016, 2016110020 (doi: 10.20944/preprints201611.0020.v1). Su, X.; Rehman, L.; Guo, H.; Li, X.; Cheng, H. AAC as a Potential Target Gene to Control Verticillium dahliae. Preprints 2016, 2016110020 (doi: 10.20944/preprints201611.0020.v1).

Abstract

Verticillium dahliae invades the roots of host plants and causes vascular wilt, which seriously diminishes the yield of cotton and other important crops. The protein AAC (ADP, ATP carrier) is responsible for transferring ATP from the mitochondria into the cytoplasm. When V. dahliae protoplasts were transformed with short interfering RNAs (siRNAs) targeting the VdAAC gene, fungal growth and sporulation were significantly inhibited. To further confirm a role for VdAAC in fungal development, we generated knockout mutants (ΔVdACC), which were hypersensitive to stresses such as UV light and high concentrations of NaCl or sorbitol. Compared with wild-type V. dahliae  (Vd wt), ΔVdAAC was impaired in germination and virulence; these impairments were rescued in the complementary strains (ΔVdAAC-C). Moreover, when an RNAi construct of VdAAC under the control of the 35S promoter was used to transform Nicotiana benthamiana, the expression of VdAAC was downregulated in the transgenic seedlings, and they had elevated resistance against V. dahliae. The results of this study suggest that VdAAC contributes to fungal development, virulence and response to stresses and is a promising candidate gene to control V. dahliae. In addition, RNAi is a highly efficient way to silence fungal genes and provides a novel strategy to improve disease resistance in plants.

Subject Areas

Verticillium dahliae; VdAAC; RNAi; growth; virulence

Readers' Comments and Ratings (0)

Discuss and rate this article
Views 134
Downloads 121
Comments 0
Metrics 0
Discuss and rate this article

×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.