Preprint Article Version 1 NOT YET PEER-REVIEWED

Chitosan-Collagen Coated Magnetic Nanoparticles for Lipase Immobilization – New Type of “Enzyme Friendly” Polymer Shell Crosslinking with Squaric Acid

  1. Faculty of Chemistry, Nicolaus Copernicus University in Torun, Gagarina 7, 87-100 Toruń, Poland
  2. Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, dr A. Jurasza 2, 85-089 Bydgoszcz, Poland
Version 1 : Received: 27 October 2016 / Approved: 27 October 2016 / Online: 27 October 2016 (18:40:02 CEST)

How to cite: Ziegler-Borowska, M.; Chelminiak-Dudkiewicz, D.; Siódmiak, T.; Sikora, A.; Wegrzynowska-Drzymalska, K.; Skopinska-Wisniewska, J.; Kaczmarek, H.; Marszałł, M. Chitosan-Collagen Coated Magnetic Nanoparticles for Lipase Immobilization – New Type of “Enzyme Friendly” Polymer Shell Crosslinking with Squaric Acid. Preprints 2016, 2016100123 (doi: 10.20944/preprints201610.0123.v1). Ziegler-Borowska, M.; Chelminiak-Dudkiewicz, D.; Siódmiak, T.; Sikora, A.; Wegrzynowska-Drzymalska, K.; Skopinska-Wisniewska, J.; Kaczmarek, H.; Marszałł, M. Chitosan-Collagen Coated Magnetic Nanoparticles for Lipase Immobilization – New Type of “Enzyme Friendly” Polymer Shell Crosslinking with Squaric Acid. Preprints 2016, 2016100123 (doi: 10.20944/preprints201610.0123.v1).

Abstract

The synthesis of new collagen, chitosan and chitosan-collagen coated magnetic nanoparticles have been done. Two types of cross-linkers for polymer shell stabilization were used: glutaraldehyde (Gla) as a standard cross-linker and new one – squaric acid (SqA). Structure and morphology of prepared nanoparticles were characterized by ATR-FT IR, XRD and TEM analysis. The immobilization of lipase from Candida rugosa was performed on the nanoparticles surface. The amount of immobilized enzyme was quantified by the Bradford method. All of lipase-biopolymers coated nanoparticles were characterized with good activity recovery. A little hyperactivation of lipase immobilized on nanoparticles with SqA was observed. All of prepared lipase-immobilized nanoparticles were characterized with very good reusability.

Subject Areas

immobilization, lipase, magnetic nanoparticles, chitosan, collagen, squaric acic

Readers' Comments and Ratings (0)

Discuss and rate this article
Views 137
Downloads 99
Comments 0
Metrics 0
Discuss and rate this article

×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.