Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Chitosan-Collagen Coated Magnetic Nanoparticles for Lipase Immobilization – New Type of “Enzyme Friendly” Polymer Shell Crosslinking with Squaric Acid

Marta Ziegler-Borowska
* ORCID logo ,
Dorota Chelminiak-Dudkiewicz
,
Tomasz Siódmiak
,
Adam Sikora
,
Katarzyna Wegrzynowska-Drzymalska
,
Joanna Skopinska-Wisniewska
,
Halina Kaczmarek
,
Michał P. Marszałł
Version 1 : Received: 27 October 2016 / Approved: 27 October 2016 / Online: 27 October 2016 (18:40:02 CEST)

A peer-reviewed article of this Preprint also exists.

Ziegler-Borowska, M.; Chelminiak-Dudkiewicz, D.; Siódmiak, T.; Sikora, A.; Wegrzynowska-Drzymalska, K.; Skopinska-Wisniewska, J.; Kaczmarek, H.; Marszałł, M.P. Chitosan–Collagen Coated Magnetic Nanoparticles for Lipase Immobilization—New Type of “Enzyme Friendly” Polymer Shell Crosslinking with Squaric Acid. Catalysts 2017, 7, 26. Ziegler-Borowska, M.; Chelminiak-Dudkiewicz, D.; Siódmiak, T.; Sikora, A.; Wegrzynowska-Drzymalska, K.; Skopinska-Wisniewska, J.; Kaczmarek, H.; Marszałł, M.P. Chitosan–Collagen Coated Magnetic Nanoparticles for Lipase Immobilization—New Type of “Enzyme Friendly” Polymer Shell Crosslinking with Squaric Acid. Catalysts 2017, 7, 26.

Abstract

The synthesis of new collagen, chitosan and chitosan-collagen coated magnetic nanoparticles have been done. Two types of cross-linkers for polymer shell stabilization were used: glutaraldehyde (Gla) as a standard cross-linker and new one – squaric acid (SqA). Structure and morphology of prepared nanoparticles were characterized by ATR-FT IR, XRD and TEM analysis. The immobilization of lipase from Candida rugosa was performed on the nanoparticles surface. The amount of immobilized enzyme was quantified by the Bradford method. All of lipase-biopolymers coated nanoparticles were characterized with good activity recovery. A little hyperactivation of lipase immobilized on nanoparticles with SqA was observed. All of prepared lipase-immobilized nanoparticles were characterized with very good reusability.

Keywords

immobilization, lipase, magnetic nanoparticles, chitosan, collagen, squaric acic

Subject

Chemistry and Materials Science, Nanotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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