Preprint Article Version 1 NOT YET PEER-REVIEWED

Electrochemical Biosensor Using Acetobacter Enzyme for Detecting Alcohol

  1. Department of Chemistry Natural Sciences, Bogor Agricultural University, Gedung Kimia Wing 1 Lantai 3, Jl. Tanjung, Kampus IPB Darmaga, Bogor 16680, Indonesia
  2. Division of Microbiology Research Center for Biology, The Indonesian Institute of Sciences, Jalan Raya Jakarta-Bogor Km 46 Cibinong, Bogor 16911, Indonesia
Version 1 : Received: 24 October 2016 / Approved: 25 October 2016 / Online: 25 October 2016 (09:46:35 CEST)

How to cite: Iswantini, D.; Nurhidayat, N.; Djukarsa, I. Electrochemical Biosensor Using Acetobacter Enzyme for Detecting Alcohol. Preprints 2016, 2016100107 (doi: 10.20944/preprints201610.0107.v1). Iswantini, D.; Nurhidayat, N.; Djukarsa, I. Electrochemical Biosensor Using Acetobacter Enzyme for Detecting Alcohol. Preprints 2016, 2016100107 (doi: 10.20944/preprints201610.0107.v1).

Abstract

Determining halal product of fermented foods and bevearages becomes important for muslim consumers due to religious codification of halal. In order to help on site analysis, an alcohol biosensor was under development. The biosensor was constructed using bacteria producing alcohol oxidase (AOX). Bacteria from apple juice was isolated in advance to obtain pure bacteria. The bacteria was cultured in two solid media. Bacteria on the Acetobacter media had a resistance in a solution containing alcohol with a higher concentration. Acetobacter was cultured on solid and liquid media for measuring at various ethanol concentrations (0.01%-3%) with different absorbance value. Bacteria with absorbance value 0.500 had the highest oxidation current peak. Later, it was used as bioreceptor for measuring ethanol by cyclic voltammetry method. The linearity of measurement later was compared with spectrophotometric measurement. By using cyclic voltammetry the linearity had higher R2 value than spectrophotometric method. However, it only had stability for two days, there fore it needs more improvement for lasting its lifetime. Basically, electrochemical method has potency for further being improved fur as an alternative method of ethanol on-site measurement.

Subject Areas

alcohol oxidase (AOX); biosensor; halal products; cyclic voltammetry

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