Preprint Article Version 1 NOT YET PEER-REVIEWED

The Effects of Asthma Medications on Reactive Oxygen Species Production in Human Monocytes

  1. Division of Nephrology, Department of Internal Medicine, Kaohsiung Armed Forces General Hospital, Kaohsiung 802, Taiwan
  2. Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, Taiwan
  3. Department of Pediatrics, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, Taiwan
  4. Department of Laboratory Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan
  5. Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan
  6. Division of Chest Medicine, Department of Internal Medicine, Changhua Christian Hospital, Changhua 500, Taiwan
  7. Department of Respiratory Care, College of Health Sciences, Chang Jung Christian University, Tainan 711, Taiwan
  8. School of Medicine, Chung Shan Medical University, Taichung 402, Taiwan
  9. Department of Radiation Oncology, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan
  10. Ta-Kuo Clinic, Kaohsiung, Taiwan
  11. Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan
  12. Department of Pediatrics, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung 812, Taiwan
  13. Department of Pediatrics, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan
  14. Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung 804, Taiwan
Version 1 : Received: 24 October 2016 / Approved: 24 October 2016 / Online: 24 October 2016 (05:50:30 CEST)

How to cite: Tsai, M.; Lin, Y.; Lin, C.; Huang, M.; Lee, M.; Kuo, C.; Hung, C.; Kuo, P. The Effects of Asthma Medications on Reactive Oxygen Species Production in Human Monocytes. Preprints 2016, 2016100101 (doi: 10.20944/preprints201610.0101.v1). Tsai, M.; Lin, Y.; Lin, C.; Huang, M.; Lee, M.; Kuo, C.; Hung, C.; Kuo, P. The Effects of Asthma Medications on Reactive Oxygen Species Production in Human Monocytes. Preprints 2016, 2016100101 (doi: 10.20944/preprints201610.0101.v1).

Abstract

Asthma is a chronic inflammatory airway disease induced by many environmental factors. The inhalation of allergens and pollutants promote the reactive oxygen species (ROS) production leading to airway inflammation, hyper-responsiveness and remodeling in allergic asthma. The effects of asthma medications on ROS production are unclear. The present study investigated the anti-ROS effects of current asthma medications including inhaled corticosteroid (ICS; budesonide and fluticasone), leukotriene receptor antagonist (LTRA; montelukast), long acting β2 agonists (LABAs; salmeterol and formoterol) and a new extra-LABA (indacaterol). The human monocyte cell line THP-1 cells were pre-treated with different concentrations of the asthma medications at different time-points after hydrogen peroxide (H2O2) stimulation. H2O2 production was measured with DCFH-DA by flow cytometry. Montelukast, fluticasone and salmeterol suppressed H2O2-induced ROS production. Indacaterol enhanced H2O2-induced ROS production. Budesonide and formoterol alone had no anti-ROS effects, but the combination of these two drugs significantly suppressed H2O2-induced ROS production. Different asthma medications have different anti-ROS effects on monocytes. The combination therapy with LABA and ICS seemed not be the only choice for asthma control. Montelukast may be also a good supplemental treatment for the poorly-controlled asthma because of its powerful anti-ROS effects. Our findings provide a novel therapeutic view in asthma.

Subject Areas

reactive oxygen species (ROS); asthma; montelukast; long-acting β2 agonist (LABA); corticosteroid; monocyte

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