Preprint Article Version 1 NOT YET PEER-REVIEWED

RNA-seq Analysis of Solanesol Biosynthesis Genes in Nicotiana tabacum

  1. Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China
  2. Guelph Food Research Center, Agriculture and Agri-Food Canada, Guelph, ON N1G 5C9, Canada
  3. Department of Biology, University of Virginia, Charlottesville, VA 22904, USA
Version 1 : Received: 18 September 2016 / Approved: 18 September 2016 / Online: 18 September 2016 (10:45:27 CEST)

A peer-reviewed article of this Preprint also exists.

Yan, N.; Zhang, H.; Zhang, Z.; Shi, J.; Timko, M.P.; Du, Y.; Liu, X.; Liu, Y. Organ- and Growing Stage-Specific Expression of Solanesol Biosynthesis Genes in Nicotiana tabacum Reveals Their Association with Solanesol Content. Molecules 2016, 21, 1536. Yan, N.; Zhang, H.; Zhang, Z.; Shi, J.; Timko, M.P.; Du, Y.; Liu, X.; Liu, Y. Organ- and Growing Stage-Specific Expression of Solanesol Biosynthesis Genes in Nicotiana tabacum Reveals Their Association with Solanesol Content. Molecules 2016, 21, 1536.

Journal reference: Molecules 2016, 21, 1536
DOI: 10.3390/molecules21111536

Abstract

Solanesol is a noncyclic terpene alcohol composed of nine isoprene units and it mainly accumulates in solanaceous plants, especially tobacco (Nicotiana tabacum L.). Here, RNA-seq analyses of tobacco leaves, stems, and roots were used to identify solanesol biosynthesis genes. Six 1-deoxy-d-xylulose 5-phosphate synthase, two 1-deoxy-d-xylulose 5-phosphate reductoisomerase, two 2-C-methyl-d-erythritol 4-phosphate cytidylyltransferase, four 4-diphosphocytidyl-2-C-methyl-d-erythritol kinase, two 2-C-methyl-d-erythritol 2,4-cyclodiphosphate synthase, four 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase, two 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase, six isopentenyl diphosphate isomerase, and two solanesyl diphosphate synthase (SPS) genes were identified to be involved in solanesol biosynthesis. Furthermore, the two N. tabacum SPS (NtSPS1 and NtSPS2), which had two conserved aspartate-rich DDxxD domains, were highly homologous with SPS enzymes from other solanaceous plant species. In addition, the solanesol contents of three organs, and leaves from four growing stages, corresponded with the distribution of chlorophyll. Our findings provide a comprehensive evaluation of the correlation between the expression of different biosynthetic genes and the accumulation of solanesol in tobacco.

Subject Areas

Nicotiana tabacum; solanesol; RNA-seq; solanesyl diphosphate synthase; gene expression; chlorophyll

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