Preprint Article Version 1 NOT YET PEER-REVIEWED

Antioxidant Activity and Isolation of β-Sitosterol from Ethyl Acetate Extract of Aerial Parts of Consolida orientalis

  1. Department of Biology, Islamic Azad University, Qaemshhahr Branch, Qaemshahr, Iran
Version 1 : Received: 9 September 2016 / Approved: 9 September 2016 / Online: 9 September 2016 (11:18:02 CEST)

How to cite: Dehpour, A. Antioxidant Activity and Isolation of β-Sitosterol from Ethyl Acetate Extract of Aerial Parts of Consolida orientalis. Preprints 2016, 2016090037 (doi: 10.20944/preprints201609.0037.v1). Dehpour, A. Antioxidant Activity and Isolation of β-Sitosterol from Ethyl Acetate Extract of Aerial Parts of Consolida orientalis. Preprints 2016, 2016090037 (doi: 10.20944/preprints201609.0037.v1).

Abstract

General phytochemical screening of the aerial parts of Consolida orientalis revealed the presence of steroids, terpenes, phenolic compounds, saponins, fatty acids, alkaloids. This study was conducted to investigate the bioactivities of extracts, isolation and identification the compounds from aerial parts of C.orientalis. The main goal of the present study is identifying and characterizing the antioxidant activity of the Consolida orientalis and biological isolation of active terpenoid. Aerial parts of the plant were dried at room temperature and reduced to small pieces, followed by using extraction with ethyl acetate percolation. Tree complementary analysis system was used, DPPH free radical scavenging test, total phenolic metabolites and FRAP. The total phenolic content was 38.83±2.09 mg gallic acid corresponding to g-1 extract with regarding to standard curve (y=0.0054x+0.0488, r2=0.995). IC50 value for DPPH radical – scavenging was 987.11±28.66 mgml-1. The extract was exhibited a medium reducing power compared with Vit C. The isolation and purification was afforded white crystalline powder which was subjected to physical, chemical and spectral identification by IR, 1H- and 13C- NMR and GC-MS. Isolated compound was identified as β-sitosterol. That is a terpenoid with melting point 133.4-134.5 ͦ c and with molecular formula C29H50O.

Subject Areas

Consolida orientalis; ethyl acetate extract; antioxidant activity; β-sitosterol

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