Preprint Article Version 1 NOT YET PEER-REVIEWED

Anticancer and Antioxidant Activities of Some Algae from Western Libyan Coast

  1. Chemistry Department, Faculty of Sciences, University of Tripoli, Tripoli, Libya
  2. Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey, United Kingdom GU2 7XH
Version 1 : Received: 3 September 2016 / Approved: 5 September 2016 / Online: 5 September 2016 (14:23:52 CEST)

How to cite: Alghazeer, R.; Enaeli, M.; Howell, N. Anticancer and Antioxidant Activities of Some Algae from Western Libyan Coast. Preprints 2016, 2016090018 (doi: 10.20944/preprints201609.0018.v1). Alghazeer, R.; Enaeli, M.; Howell, N. Anticancer and Antioxidant Activities of Some Algae from Western Libyan Coast. Preprints 2016, 2016090018 (doi: 10.20944/preprints201609.0018.v1).

Abstract

Seaweeds are considered as one of the largest biomass producers in marine environment that is rich in bioactive metabolites and a source of natural ingredients for functional foods. The potential antioxidant activity and the potential inhibition of Caco2 cell proliferation, of crude extracts of: Chlorophyta (Ulva lactuca, and Codium tomentosum), Phaeophyta (Cystoseira crinita, Cystoseira stricta, and Sargassum vulgare), and Rhodophyta (Gelidium latifolium, Hypnea musciformis, and Jania rubens) collected from western Libyan coast were evaluated in vitro. The antioxidant activity was determined by reducing power and DPPH assays while cell proliferation, morphological changes and the cell cycle arrest were assessed by MTT, inverted light microscope and flow cytometry methods respectively. The polyphenols and flavonoids rich extracts showed remarkable reducing power and antiradical properties. After exposure of Caco2 cells to; various concentrations of extracts (50, 100,150 and 200 µg/mL) especially from brown algae for 72 h, significantly reduced cell proliferation. The antiproliferative effect of algae extracts was correlated with their polyphenol and flavonoid contents. Cell cycle analysis further showed that cells were arrested in G phases along with an increment in sub-diploidal cell population (sub-G) after extract application. These results imply that seaweeds which are rich in bioactive compounds may be in anticancer drug research programs. However, further investigations are essential to reveal the molecular mechanisms of the anticancer activities of these algae.

Subject Areas

polyphenols, flavonoids, seaweeds, antioxidant activity, anticancer activity

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