Preprint Article Version 2 NOT YET PEER-REVIEWED

Quantitative Detection of Trace Malachite Green in Aquiculture Water Samples by Extractive Electrospray Ionization Mass Spectrometry

  1. Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China Institute of Technology, Nanchang 330013, China
  2. Department of Electronic and Information Engineering, Harbin Institute of Technology, Weihai 264209, China
Version 1 : Received: 8 August 2016 / Approved: 8 August 2016 / Online: 8 August 2016 (14:58:11 CEST)
Version 2 : Received: 10 August 2016 / Approved: 11 August 2016 / Online: 11 August 2016 (10:00:17 CEST)

A peer-reviewed article of this Preprint also exists.

Fang, X.; Yang, S.; Chingin, K.; Zhu, L.; Zhang, X.; Zhou, Z.; Zhao, Z. Quantitative Detection of Trace Malachite Green in Aquiculture Water Samples by Extractive Electrospray Ionization Mass Spectrometry. Int. J. Environ. Res. Public Health 2016, 13, 814. Fang, X.; Yang, S.; Chingin, K.; Zhu, L.; Zhang, X.; Zhou, Z.; Zhao, Z. Quantitative Detection of Trace Malachite Green in Aquiculture Water Samples by Extractive Electrospray Ionization Mass Spectrometry. Int. J. Environ. Res. Public Health 2016, 13, 814.

Journal reference: Int. J. Environ. Res. Public Health 2016, 13, 814
DOI: 10.3390/ijerph13080814

Abstract

Exposure to malachite green (MG) may pose great health risks to humans, thus it is of prime importance to develop fast and robust methods to quantitatively screen the presence malachite green in environment. Herein application of extractive electrospray ionization mass spectrometry (EESI-MS) has been extended to the trace detection of MG within lake water and aquiculture water, due to the intensive use of MG as biocide in fisheries. This method has the advantage of obviating offline liquid-liquid extraction or tedious matrix separation prior to the measurement of malachite green in native aqueous medium. The experimental results indicate that the extrapolated detection limit for MG was ~3.8 ug L-1 (S/N=3) in lake water samples and ~ 0.5 ug L-1 in ultrapure water under optimized experimental conditions. The signal intensity of MG showed good linearity over the concentration range of 10–5000 ug L-1. Measurement of practical water samples fortified with MG at 0.01 and 0.1 mg L-1 gave a good validation of the established calibration curve. The average recoveries of malachite green in lake water and Carassius carassius fish farm effluent water were 114.9% (6.6% RSD) and 85.4% (9.2% RSD), respectively. Overall, the established EESI-MS/MS method has been demonstrated suitable for sensitive and rapid (<2 min per sample) quantitative detection of malachite green in various aqueous media, indicating its potential for online real-time monitoring of real life samples.

Subject Areas

extractive electrospray ionization; rapid detection; malachite green; water; mass spectrometry

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