Acute lung injury (ALI) is characterized by inflammation of the lung tissue and oxidative injury caused by excessive accumulation of reactive oxygen species. Studies have suggested that anti-inflammatory or antioxidant agents could be used for the treatment of ALI with a good outcome. Therefore, our study aimed to test whether the mycelium extract of Sanghuangporus sanghuang (SS-1), believed to exhibit antioxidant and anti-inflammatory properties, could be used against the excessive inflammatory response associated with LPS-induced ALI in mice and to investigate its possible mechanism of action. The experimental results showed that the administration of SS-1 could inhibit LPS-induced inflammation. SS-1 could reduce the number of inflammatory cells, inhibit MPO activity, regulate the TLR4/PI3K/Akt/mTOR pathway and the signal transduction of NF-κB and MAPK pathways in the lung tissue, and inhibit HNGB1 activity in BALF. In addition, SS-1 could affect the synthesis of antioxidant enzymes HO-1 and Trx-1 in the lung tissue and regulate signal transduction in the KAP1/Nrf2/Keap1 pathway. Histological results showed that administration of SS-1 prior to induction could inhibit the large-scale LPS-induced neutrophil infiltration of the lung tissue. Therefore, based on all experimental results, we propose that SS-1 exhibits a protective effect against LPS-induced (ALI) in mice. The mycelium of S. sanghuang can potentially be used for the treatment or prevention of inflammation-related diseases

Plastic waste management has become a major problem in the present times, and many countries are struggling to deal with it. The situation has become a crisis in developing nations due to a lack of proper waste management, resources, technology, and political will. The extensive usage of foam-based packaging materials such as Styrofoam as a secondary packaging material has led to environmental pollution both in land and water, in developing countries. It is important to address this problem using a sustainable approach such as biomimetic manufacturing. One such solution that is present in developed nations is mycelium-based packaging, wherein the agricultural and industrial-based cellulose waste is converted into a biocomposite with the help of a fungi root network. Such a process has the potential to convert biomass into useful products but also has an indirect effect on pollution reduction by eliminating stubble burning in nations like India. In this review, we examine the details of the manufacturing process, properties, advantages, and limitations of such composite materials. A comparison of the present status of such materials produced in the market by some of the companies across the world is presented. Finally, the challenges involved with such materials and the future directions are discussed.

Wood plastic composite usage and demands have increased due to chemical, and mechanical properties when compared to plastic materials. However, because of the possibility of structural and mechanical changes of the material when exposed to external environment more research needs to be done. In the present study yellow birch/HDPE composites materials made by injection molding were treated by zinc oxide and exposed to fungal rots. Mechanical properties of the composites were assessed by Tensile and Izod impact test. The impact energies of the samples loaded with 30 % ZnO-treated fibers and exposed to G. trabeum and T. versicolor decreased, compared to when samples were not treated with ZnO. The mechanical properties of all samples treated with ZnO and exposed to rot decreased which were reported as a decreased Young's modulus and impact energies. ZnO prevented mycelium proliferation which was nonexistent on samples. It has been noted that the decrease in mechanical properties of treated samples was due to NaOH used to dissolve the ZnO powder.

Truffles are ascomycete hypogeous fungi belonging to the Tuberaceae family of the Pezizales order that grow in ectomycorrhizal symbiosis with tree roots and are known for their peculiar aromas and flavors. Axenic culture of truffle mycelium is problematic because it is not possible in many cases, and the growth rate is meager when it is possible. This limitation prompts searching and characterizing new strains that can be handled in laboratory conditions for basic and applied studies. In this work, a new strain of Tuber borchii (strain SP1) has been isolated and cultured, and its transcriptome has been analyzed under different in vitro culture conditions. The results show that the best T. borchii SP1 growth was obtained using maltose-enriched cultures made with soft-agar and in static submerged cultures made at 22ºC. The transcriptome analysis of this strain cultured in different media indicated that most of the gene transcription effort is due to a limited number of genes (20% of genes account for 80% of the transcription), that the transcription profile of the central metabolism genes was similar in the different conditions analyzed with a transcription signal detected for around 80% of the annotated genes. The gene expression profile suggests that T. borchii uses a fermentative rather than respiratory metabolism, even in aerobic conditions. Finally, there is a reduced expression of genes belonging to secondary metabolite clusters, whereas there is a significative transcription of those involved in producing volatile aromatic compounds.