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Preprint ARTICLE | doi:10.20944/preprints202304.1235.v1

Effect of the Incorporation of GWAS-Selected Markers in Genomic Selection, Study Model: Flavonoid Pigmentation Traits in Sorghum

Anngie Hernandez

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Genomic prediction; flavonoid pigmentation; Sorghum bicolor; prediction accuracy; marker-assisted selection

Online: 29 April 2023 (10:11:10 CEST)

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Marker-assisted selection (MAS) and genomic selection (GS) have been used to select individuals with desirable traits. MAS used a few markers associated with a specific trait to select individuals with desirable traits, which are determined after a Genome-wide association studies (GWAS). On the contrary, GS uses a large number of markers distributed across the genome to predict the genomic breeding values for a further selection of the individuals. In general, MAS has shown a high prediction accuracy but is not suitable for traits that are controlled for multiple genes, and has another constraint, it is required the phenotypic data; on the contrary, GS has not shown the highest prediction accuracy as MAS but it takes into account the effect of multiple genes controlling a target trait and it can be used without phenotypic data. Including GWAS-selected markers in GS can enhance the reduced prediction accuracy that GS shows in comparison with MAS. Thus, the objective of this study was to compare the prediction accuracy of MAS, and some models of genomic prediction (gBLUP, gBLUP including GWAs-selected markers, and some Bayesian models such as Bayes A, Bayes B, Bayes LASSO and Bayesian Ridge Regression) with GWAS-selected markers incorporated in gBLUP in order to confirm if the incorporation of GWAS in GS increases the prediction accuracy of GS. As a model for this study, it was used data from Sorghum which has shown population structure, to evaluate if the incorporation of GWAs-selected markers into GS improves prediciton accuracy. It was used a sample of 6000 SNPs out of the 265.487 reported in the study conducted by Morris et al (2013), and also it was considered some parameters that affect the efficiency of the selection such as the size of the training population, the heritability, and the number of QTNs. The GWAS-selected SNPs were identified after using the model BLINK. The results showed that the incorporation of GWAS-selected markers enhanced the performance of the genomic selection with similar prediction accuracy as MAS, the number of QTNs and size of the training population affected the accuracy, with higher accuracy with a bigger size of the training population and with a lower number of QTNs, but it seems that the heritability does not have any impact in the model where GWAS-selected SNPs were included in gBLUP.

Preprint ARTICLE | doi:10.20944/preprints202103.0132.v1

Marker Assisted Selection of Malic-Consuming Saccharomyces cerevisiae Strains for Winemaking. Efficiency and Limits of a QTL’s Driven Breeding Program

Charlotte Vion, Emilien Peltier, Margaux Bernard, Maitena Muro, Philippe Marullo

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Wine yeast; malic acid; pH; breeding; Malo Lactic Fermentation; Marker Assisted Selection

Online: 3 March 2021 (12:43:08 CET)

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Preprint ARTICLE | doi:10.20944/preprints201907.0166.v1

Genome Wide Association Studies for Four Physiological Traits in Groundnut (Arachis hypogaea L.) Minicore Collection

Abdulwahab Shaibu, Clay Sneller, Babu Motagi, Jackline Chepkoech, Mercy Chepngetich, Zainab Miko, Adamu Isa, Hakeem Ajeigbe, Sanusi Mohammed

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: DArTseq; Groundnut; Linkage disequilibrium; Marker assisted selection; Marker trait association; Physiological traits

Online: 12 July 2019 (11:42:33 CEST)

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Preprint REVIEW | doi:10.20944/preprints201912.0158.v1

A Review of Important QTLs for Biofortification Traits in Rice

Vinay Sharma, Dinesh Kumar Saini, Asish Kumar, Prashant Kaushik

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: QTLs; biofortification; malnutrition; hidden hunger; marker-assisted breeding

Online: 12 December 2019 (03:52:07 CET)

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Preprint ARTICLE | doi:10.20944/preprints202305.0043.v1

Inclusion of Significant Markers Identified With GWAS Into Prediction Models

Olufunke Ayegbidun

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Genome-Wide Association Study; Genomic Selection, Marker-Assisted Selection

Online: 2 May 2023 (02:33:16 CEST)

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Preprint REVIEW | doi:10.20944/preprints202011.0390.v1

Self Incompatibility, Inbreeding Depression, and Potential to Develop Inbred Lines in Alfalfa: A Review

Atit Parajuli, Longxi Yu, Michael Peel, Deven See, Steven Wagner, Steven Norberg, Zhiwu Zhang

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Hybrid Vigor; Heterosis; Bulked Segregant Analysis; Marker Assisted Selection

Online: 13 November 2020 (15:47:40 CET)

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Preprint REVIEW | doi:10.20944/preprints202008.0088.v1

Gene Pyramiding for Sustainable Crop Improvement against Biotic and Abiotic Stresses

Richard Dormatey, Chao Sun, Kazim Ali, Jeffrey A Coulter, Zhenzhen Bi, Jiangping Bai

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Gene pyramiding; marker assisted selection; stress resistance; crop improvement

Online: 4 August 2020 (11:10:22 CEST)

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Preprint REVIEW | doi:10.20944/preprints202009.0308.v1

Accelerating Tomato Breeding by Exploiting Genomic Selection Approaches

Elisa Cappetta, Giuseppe Andolfo, Antonio Di Matteo, Amalia Barone, Luigi Frusciante, Maria Raffaella Ercolano

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Tomato; genetic breeding value; training population; genotyping; marker effect; phenotyping; selection schemes

Online: 14 September 2020 (00:08:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202312.0649.v1

Genetic Transformation of Potato Using the Non-selection Approach

Dmitry Miroshnichenko, Anna Okuneva, Anna Klementyeva, Tatiana Sidorova, Vadim Timerbaev, Alexander S. Pushin, Sergey V. Dolgov

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Solanum tuberosum L.; antibiotic-free; selectable marker gene; gene silencing; translation initiation factor eIF4E

Online: 11 December 2023 (06:13:15 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.1618.v1

Development of Multi-Resistant Rice Restorer Lines and Hybrid Varieties by Pyramiding Resistance Genes against Blast and Brown Planthopper

Zhao Deng, Peng Qin, Kaiyu Liu, Nan Jiang, Tianze Yan, Xuanwen Zhang, Chenjian Fu, Guangcun He, Kai Wang, Yuanzhu Yang

Subject: Biology And Life Sciences, Plant Sciences Keywords: blast; brown planthopper; resistance gene; marker-assisted selection; multiplex PCR; hybrid rice

Online: 26 March 2024 (16:44:05 CET)

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Preprint REVIEW | doi:10.20944/preprints202404.1462.v1

The Kirkhouse Trust: Successes and Challenges in Twenty Years of Supporting Independent, Contemporary Grain Legume Breeding Projects in India and African Countries

Claudia Canales Holzeis, Paul Gepts, Robert Koebner, Prem N Mathur, Sonia Morgan, María Muñoz Amatriaín, Travis Parker, Edwin M Southern, Michael P Timko

Subject: Agricultural Science And Agronomy, Biology And Life Sciences Keywords: legumes; crop breeding; Africa; cowpea; common bean; dolichos; Bambara groundnut; marker assisted selection

Online: 23 April 2024 (13:06:57 CEST)

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Preprint REVIEW | doi:10.20944/preprints202004.0138.v1

Honey Bee Survival Mechanisms Against the Parasite Varroa destructor: A Systematic Review of Phenotypic and Genomic Research Efforts

Fanny Mondet, Alexis Beaurepaire, Alison McAfee, Barbara Locke, Cedric Alaux, Solene Blanchard, Bob Danka, Yves Le Conte

Subject: Biology And Life Sciences, Parasitology Keywords: host resistance; tolerance; honey bee; Varroa destructor; marker assisted selection; host-parasite interactions

Online: 9 April 2020 (06:06:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.0280.v1

Mapping of Stripe Rust Resistance Gene Yr72 in Two Common Wheat Landraces

Mumta Chhetri, Hanif Miah, Debbie Wong, Matthew Hayden, Urmil Bansal, Harbans Bariana

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: wheat; stripe rust; KASP marker; STS marker; marker validation

Online: 5 October 2023 (14:27:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201807.0313.v1

Identification of volatile compounds and selection of discriminant markers for elephant dung coffee using static headspace gas chromatography–mass spectrometry and chemometrics

Poowadol Thammarat, Chadin Kulsing, Kanet Wongravee, Natchanun Leepipatpiboon, Thumnoon Nhujak

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: elephant dung coffee; volatile compound; discriminant marker; SHS GC–MS; chemometrics; coffee authentication

Online: 17 July 2018 (15:02:03 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.1268.v1

Resistance to Multiple Foliar Fungal Diseases in Tomato

Dilip R. Panthee, Anju Pandey, Rajan Paudel

Subject: Biology And Life Sciences, Horticulture Keywords: Foliar fungal diseases; Integrated disease management; Marker-assisted selection; Quantitative trait loci; Resistance breeding; Solanum lycopersicum; Tomato

Online: 19 June 2023 (03:14:19 CEST)

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Preprint REVIEW | doi:10.20944/preprints202010.0453.v2

Oocyte Selection for in vitro embryo Production in Bovine Species: Non-Invasive Approaches for the New Challenges of Oocyte Competence

Luis Aguila, Favian Treulen, Jacinthe Therrien, Ricardo Felmer, Martha Valdivia, Smith Lawrence C

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: oocyte competence; livestock production; assisted reproductive technology; embryo development; micromanipulation; in vitro production

Online: 17 November 2020 (12:41:51 CET)

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Preprint HYPOTHESIS | doi:10.20944/preprints202006.0314.v2

Semen Quality as a Potential Susceptibility Indicator to SARS-CoV-2 Insults in Polluted Areas

Luigi Montano, Ian Marc Bonapace, Francesco Donato, Pietro Massimiliano Bianco, Antonino Guglielmino, Marina Piscopo

Subject: Medicine And Pharmacology, Urology And Nephrology Keywords: air pollution; COVID-19; semen quality; environmental marker; health marker; oxidative stress

Online: 6 July 2020 (09:48:39 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202312.0168.v1

Cancer Screening in Angolan Individuals Using Tumor Marker Tests at Mediag, Luanda-Angola

Edson Kutelela Cassinela, Lourenço Quixari, Silvana da Rocha Silveira, Cruz dos Santos Sebastião, António Armando, Carlos Pinto-Sousa, José Fernando, Manuel Sangombe Epalanga, Euclides Nenga Manuel Sacomboio

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: tumor marker; screening; Angolan population

Online: 4 December 2023 (11:40:55 CET)

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Preprint ARTICLE | doi:10.20944/preprints202305.2204.v1

Genome-Wide Association Mapping of Oil Content and Seed Related Traits in Shea Tree (Vitellaria paradoxa subsp. nilotica) Population

Juventine Boaz Odoi, Emmanuel Amponsah Adjei, Michael Teye Barnor, Richard Edema, Samson Gwali, Danguah Agyemang, Thomas Lapaka Odong, Prasad S. Hendre

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Linked; marker association; annotation; Genes

Online: 31 May 2023 (08:41:16 CEST)

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Working Paper ARTICLE

Inducible Selectable Marker Genes to Improve Aspergillus fumigatus Genetic Manipulation

Clara Baldin, Alexander Kühbacher, Petra Merschak, Luis Enrique Sastré-Velásquez, Beate Abt, Anna-Maria Dietl, Hubertus Haas, Fabio Gsaller

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: dominant selectable marker; Aspergillus fumigatus; inducible marker; hph; ptrA; xylanase promoter; thiamine; hygromycin; pyrithiamine

Online: 24 May 2021 (13:22:44 CEST)

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Preprint REVIEW | doi:10.20944/preprints201912.0242.v1

A Systematic Literature Review of Animal Assisted Interventions in Oncology (Part I): Methods and Results

Timothy Holder, Margaret Gruen, David Roberts, Tamara Somers, Alper Bozkurt

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: animal-assisted interventions; animal-assisted activities; animal-assisted therapy; oncology; cancer; human-animal bond; quantitative

Online: 19 December 2019 (06:41:38 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.0209.v1

Management of Donkeys in Assisted Interventions: a Snapshot

Lucia Sobrero, Emanuela Dalla Costa, Michela Minero

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: animal welfare; donkey welfare; animal-assisted interventions; donkey-assisted interventions

Online: 5 February 2024 (03:45:41 CET)

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Preprint REVIEW | doi:10.20944/preprints201912.0243.v1

A Systematic Literature Review of Animal Assisted Interventions in Oncology (Part II):Theoretical Mechanisms and Frameworks

Timothy Holder, Margaret Gruen, David Roberts, Tamara Somers, Alper Bozkurt

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: animal-assisted interventions; animal-assisted activities; animal-assisted therapy; oncology; cancer; human-animal bond; mechanisms; theoretical frameworks

Online: 19 December 2019 (06:45:23 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.1564.v1

Individual progression of S100 calcium-binding protein beta as a surrogate for epilepsy risk: rationale and design of a meta-analysis project

Jianxun He, Beilei Li, Chuhan Miao

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: S100B; Epilepsy; Biomarker; Diagnostic marker; Prognostic value

Online: 27 March 2024 (01:45:03 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.0593.v1

Primary Mosaic Neuroendocrine-/Adenocarcinoma of The Prostate: Clinical and Histological Description

Kálmán Almási, Noémi Kránitz, Tamás Kullmann

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: neuroendocrine; mosaic; prostate cancer; immunohistochemistry; tumour marker

Online: 9 November 2023 (07:24:21 CET)

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Preprint ARTICLE | doi:10.20944/preprints202309.0936.v1

Mosaic Neuroendocrine-/Adenocarcinoma of the Prostate: Clinical and Histological Description

Kálmán Almási, Noémi Kránitz, Tamás Kullmann

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: neuroendocrine; mosaic; prostate cancer; immunohistochemistry; tumour marker

Online: 14 September 2023 (04:55:17 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201906.0088.v1

LC-HRMS Screening and Identification of Novel Peptide Markers of Ricin Based on Multiple Protease Digestion Strategies

Long-Hui Liang, Chang-Cai Liu, Bo Chen, Long Yan, Hui-Lan Yu, Yang Yang, Ji-Na Wu, Xiao-Sen Li, Shi-Lei Liu

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: ricin; marker peptides; unambiguous identification; mass spectrometry

Online: 11 June 2019 (08:27:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.1055.v1

Identification and Transformation of a New Powdery Mildew Resistance Gene PmCAHM From Landrace Changanhongmai Into Common Wheat

Xueyan Chen, Yongfu Wang, Guohao Han, Jianzhong Fa, Qingqing Tan, Guoxia Liu, Hong Zhang, Yajuan Wang

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: common wheat; powdery mildew; molecular marker; genetic population

Online: 20 February 2024 (04:32:10 CET)

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Preprint REVIEW | doi:10.20944/preprints202306.0108.v1

Fight the Fire: Association of Cytokine Genomic Markers and Suicidal Behavior May Pave the Way for Future Therapies

Xenia Gonda, Gianluca Serafini, Peter Dome

Subject: Medicine And Pharmacology, Psychiatry And Mental Health Keywords: suicide; neuroinflammation; cytokine, biomarker; genomic marker; precision psychiatry

Online: 1 June 2023 (15:45:30 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202211.0244.v1

Screening Aromatic Rice Genotypes by RAPD Markers for Breeding Salinity Stress Tolerant Rice Variety

Md Abdul Azim, Md Mahbubur Rahman, Dipali Rani Gupta, Aniruddha Sarker, Tofazzal Islam

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: aromatic rice; salt screening; RAPD marker; genetic diversity

Online: 14 November 2022 (07:43:36 CET)

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Preprint REVIEW | doi:10.20944/preprints202201.0310.v1

DNA Barcoding in Plants and Animals: A Critical Review

Sheikh Sunzid Ahmed

Subject: Biology And Life Sciences, Plant Sciences Keywords: DNA barcoding; metabarcoding; BOLD; taxonomy; biodiversity; molecular marker.

Online: 20 January 2022 (15:15:36 CET)

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Preprint REVIEW | doi:10.20944/preprints202001.0370.v1

CCL11 or Eotaxin-1: An Immune Marker for Ageing and Accelerated Ageing in Neuro-psychiatric Disorders

Mariya Ivanovska, Zakee Abdi, Marianna Murdjeva, Danielle Macedo, Annabel Maes, Michael Maes

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: eotaxin; immune marker; neuroinflammation; ageing; neuro-psychiatric disorder

Online: 30 January 2020 (14:42:45 CET)

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Preprint ARTICLE | doi:10.20944/preprints201609.0071.v1

Characterization and Development of EST-SSR Markers in Sweet Potato (Ipomoea batatas (L.) Lam)

Jin-Hee Kim, Jun-Hoi Kim, Won-Sam Jo, Jung-Gwan Ham, Il Kyung Chung, Kyung-Min Kim

Subject: Biology And Life Sciences, Plant Sciences Keywords: EST-SSR marker; hexaploid; sweet potato; polymorphic; sequence

Online: 20 September 2016 (11:35:51 CEST)

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According to statistics from the Food and Agriculture Organization, the world population will increase to about 91 million (Asia 51 million, Africa 19 million). A rise in the world’s population means an increased need for food. However, climate change has caused desertification and unpredictable weather, creating problems in the supply and demand of food. Sweet potato (Ipomoea batatas) is an alternative to solving the food problem, as it is one of the world’s most important food crops, especially in developing countries. The tuberous roots of sweet potato are usually used as staple food, animal feed, industrial material, or raw material for alcohol production. In the future, more variations of sweet potato will be needed for breeding this crop. Recently, molecular markers developed for sweet potato have demonstrated good potential for use in genetic selection. In this study, a cDNA library was constructed from the total RNA of sweet potato leaves. A total of 789 copies of the cDNA were cloned in Escherichia coli by employing the pGEM-T Easy vector. Sequencing was carried out by Solgent Co. (Korea). As many as 579 expressed sequence tag–simple sequence repeat (EST-SSR) markers were designed (73.38%) from the known cDNA nucleotide base sequences. The lengths of the developed EST-SSR markers ranged from 100 to 499 bp (average length 238 bp). Their motif sequence types were varied, with most being dinucleotides and pentanucleotides, and the most commonly found motifs were CAGAAT (29.0%) and TCT (2.8%). Based on these SSR-containing sequences, 619 pairs of high-quality SSR primers were designed using WebSat and Primer3web. The total number of primers designed was 144. Polymorphism was evident in 82 EST-SSR markers among 20 Korean sweet potato cultivars tested and in 90 EST-SSR markers in the two parents of a mapping population, Yeseumi and Annobeny. In this study, the hexaploid sweet potato (2n = 6x = 90) EST-SSR markers were developed in the absence of full-sequence data. Moreover, by acting as a molecular tag for particular traits, the EST-SSR marker can also simultaneously identify information about the corresponding gene. These EST-SSR markers will allow the molecular analysis of sweet potato to be done more efficiently. Thus, we can develop high-quality sweet potato while overcoming the challenges from climate change and other unfavorable conditions.

Preprint ARTICLE | doi:10.20944/preprints202305.2136.v1

Dog Assisted Therapy Is Hypothesized to Lower Stress in Children With Autism Spectrum Disorder (ASD) And Children With Down Syndrome (DS), Which May Be Visible on a Physiological Level. In This Study, We Measured Heart Rate Variability (HRV) And Salivary

Richard Eric Griffioen, Geert J.M. Van Boxtel, Theo Verheggen, Marie-Jose Enders-Slegers, Steffie Van der Steen

Subject: Social Sciences, Behavior Sciences Keywords: Animal Assisted Interventions; HRV; Cortisol; Stress; Dog Assisted Therapy; Down Syndrome; Autism Spectrum Disorder

Online: 30 May 2023 (12:20:55 CEST)

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Preprint REVIEW | doi:10.20944/preprints201708.0084.v2

Treatment, Palliative Care or Euthanasia? Comparing End of Life Issues in Human and Veterinary Medicine

Ruth E. Eyre-Pugh

Subject: Medicine And Pharmacology, Veterinary Medicine Keywords: euthanasia; veterinary ethics; medical ethics; end-of-life; assisted suicide; palliative care; assisted dying

Online: 7 December 2017 (05:20:50 CET)

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Preprint ARTICLE | doi:10.20944/preprints202112.0179.v1

Anti-diabetic, Antioxidants, Anti-hepatorenaltoxicity Activities of Cyperus rotundus Rhizome Extract in Alloxan-induced Diabetic Rats

Mohammed Al-Awar, Turki Alqabbani

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Cyperus rotundus rhizomes; Diabetes; Lipids Profile; Hepatorenalprotective; Oxidative marker

Online: 10 December 2021 (13:11:59 CET)

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Preprint ARTICLE | doi:10.20944/preprints201902.0177.v1

Clinico-Histopathological and Immunohistochemistry Study of Ruminant’s Cutaneous Papillomavirus in Al-Muthanna Veterinary Hospital/ Iraq

Ahmed H. Al-Dabhawi, karima A. Al-Salihi, Ibrahim A. Erzuki, Tho Alfiqar H. Ali

Subject: Biology And Life Sciences, Virology Keywords: Bovine cutaneous papilloma, Iraq, Immunohistochemical (IHC), hyperkeratosis, p53 marker.

Online: 19 February 2019 (11:04:23 CET)

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Preprint CASE REPORT | doi:10.20944/preprints202310.1428.v1

Full Mouth Rehabilitation Using the All-on-Four Technique and Computer-Guided Surgery: A Case Report

ayham darwich, hasan nazha, khaldoun darwich, Asmaa Alammar

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Rehabilitation; Tooth Loss; Surgery; Computer-Assisted.

Online: 23 October 2023 (10:05:31 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201811.0104.v1

Effects of Heat Treatment on Morphology, Texture and Mechanical Properties of a MnSiAl Multiphase Steel with TRIP Behavior

Alvaro Salinas, Alfredo Artigas, Juan Pérez-Ipiña, Felipe M. Castro-Cerda, Nelson F. Garza-Montes-de-Oca, Rafael Colás, Roumen Petrov, Alberto E. Monsalve

Subject: Chemistry And Materials Science, Metals, Alloys And Metallurgy Keywords: TRIP-assisted steel; microstructure; mechanical properties

Online: 5 November 2018 (10:37:56 CET)

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Preprint REVIEW | doi:10.20944/preprints202106.0373.v1

Metal Assisted Catalytic Etching (MACE) for Nanofabrication of Semiconductor Powders

Kurt W. Kolasinski

Subject: Chemistry And Materials Science, Nanotechnology Keywords: metal assisted etching; metal assisted catalytic etching, silicon nanowires, porous silicon, porous powders, metal nanoparticles, deposition

Online: 14 June 2021 (15:04:41 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201708.0094.v2

Treatment, Palliative Care or Euthanasia? Comparing End of Life Issues in Veterinary and Human Medicine

Ruth E. Eyre-Pugh

Subject: Medicine And Pharmacology, Veterinary Medicine Keywords: euthanasia; veterinary ethics; medical ethics; end-of-life; assisted suicide; palliative care; assisted dying; moral stress

Online: 1 December 2017 (16:58:27 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.0588.v1

Phytoene Desaturase (Pds) Gene-Derived Markers Identify “A” and “B” Genomes in Banana (Musa spp.)

Fernanda dos Santos Nascimento, Marcelly Santana Mascarenhas, Samantha Costa Boaventura, Carla Catharina Hora Souza, Andresa Priscila de Souza Ramos, Anelita de Jesus Rocha, Julianna Matos da Silva Soares, Leandro Eugenio Cardamone Diniz, Tiago Antônio De Oliveira Mendes, Claudia Fortes Ferreira, Edson Perito Amorim

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Gene composition; phytoene desaturase; molecular marker; genetic improvement; Musa spp.

Online: 8 January 2024 (11:27:51 CET)

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Preprint ARTICLE | doi:10.20944/preprints202308.0850.v1

High Accuracy and Wide Range Recognition of Micro AR Markers with Dynamic Camera Parameter Control

Daisuke Haraguchi, Ryu Miyahara

Subject: Computer Science And Mathematics, Computer Vision And Graphics Keywords: Image recognition; Micro AR marker; Camera parameter control; Iterative recognition

Online: 11 August 2023 (02:53:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.1767.v1

Usefulness of Tumor Marker Score for Prediction of Prognosis in Hepatocellular Carcinoma Patients Treated with Atezolizumab plus Bevacizumab: A Multicenter Retrospective Study

Kazunari Tanaka, Kunihiko Tsuji, Atsushi Hiraoka, Toshifumi Tada, Masashi Hirooka, Kazuya Kariyama, Joji Tani, Masanori Atsukawa, Koichi Takaguchi, Ei Itobayashi, Shinya Fukunishi, Toru Ishikawa, Kazuto Tajiri, Hironori Ochi, Hidenori Toyoda, Chikara Ogawa, Takashi Nishimura, Takeshi Hatanaka, Satoru Kakizaki, Noritomo Shimada, Kazuhito Kawata, Atsushi Naganuma, Hisashi Kosaka, Tomomitsu Matono, Hidekatsu Kuroda, Yutaka Yata, Hideko Ohama, Fujimasa Tada, Kazuhiro Nouso, Asahiro Morishita, Akemi Tsutsui, Takuya Nagano, Norio Itokawa, Tomomi Okubo, Taeang Arai, Keisuke Yokohama, Hiroki Nishikawa, Michitaka Imai, Yohei Koizumi, Shinichiro Nakamura, Hiroko Iijima, Masaki Kaibori, Yoichi Hiasa, Takashi Kumada

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: hepatocellular carcinoma; tumor marker; atezolizumab plus bevacizumab; prognosis; predictive model

Online: 26 June 2023 (09:42:06 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0551.v1

Detection of Brain Tissue Damage in CO Poisoning with a New Method

Sedat Ozbay, Mustafa Ayan, Recep Eroz, Ayhan Saritas

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Argyrophilic Nucleolus Organizer Region; Hypoxia; Brain; Carbon Monoxide Poisoning; Marker

Online: 7 June 2023 (12:11:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202201.0211.v1

The Spermidine Synthase Gene SPD1: A Novel Auxotrophic Marker for Chlamydomonas Reinhardtii Designed by Enhanced CRISPR/Cas9 Gene Editing

Robert A. Freudenberg, Luisa Wittemeier, Alexander Einhaus, Thomas Baier, Olaf Kruse

Subject: Biology And Life Sciences, Plant Sciences Keywords: microalgae; CRISPR/Cas9; gene editing; spermidine; SPD1; auxotrophy; selectable marker

Online: 14 January 2022 (11:55:22 CET)

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Working Paper ARTICLE

Associations between Dietary Pattern, Bioactive Algal Nutrients Supplementation and Metabolic Risk Markers in Japanese Women: The NAMI Pilot Study

Ngatu Roger Nlandu, Tomohiro Hirao, Mitsunori Ikeda

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Adiponectin; Adiponectin modulation inducer; C-reactive protein; Metabolic marker; Sujiaonori.

Online: 20 July 2021 (14:46:08 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202107.0415.v1

Associations between Bioactive Algal Biomaterial Supplementation, Dietary Pattern and Metabolic Risk Markers in Japanese Women

Nlandu Roger Ngatu, Tomohiro Hirao, Mitsunori Ikeda

Subject: Medicine And Pharmacology, Endocrinology And Metabolism Keywords: Adiponectin; Adiponectin modulation inducer; C-reactive protein; Metabolic marker; Sujiaonori.

Online: 19 July 2021 (14:28:52 CEST)

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Working Paper ARTICLE

Detection of Peanut Allergen by Real Time PCR: Looking For the Suitable Detection Marker as Affected by Processing

Africa Sanchiz, Paulina Sánchez-Enciso, Carmen Cuadrado, Rosario Linacero

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Real-time PCR; peanut; food allergen; chloroplast marker; DNA isolation

Online: 16 June 2021 (11:33:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201906.0195.v1

A Novel Hybrid Segmentation Method with Particle Swarm Optimization and Fuzzy C-Mean Based On Partitioning the Image for Detecting Lung Cancer

Kavitha P., Prabakaran S.

Subject: Computer Science And Mathematics, Information Systems Keywords: adaptive bilateral; marker watershed; PSO; fuzzy C-mean; GLCM; SVM

Online: 20 June 2019 (09:22:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0339.v1

Genetic Mapping, Candidate Gene Identification and Marker Validation for Host Plant Resistance to the Race 4 of Fusarium oxysporum f. sp. cubense using Musa acuminata ssp. malaccensis

Andrew Chen, Jiaman Sun, Altus Viljoen, Diane Mostert, Yucong Xie, Leroy Mangila, Sheryl Bothma, Rebecca Lyons, Eva Hřibová, Pavla Christelová, Brigitte Uwimana, Delphine Amah, Stephen Pearce, Ning Chen, Jacqueline Batley, David Edwards, Jaroslav Doležel, Peter Crisp, Allan F. Brown, Guillaume Martin, Nabila Yahiaou, Angelique D’Hont, Lachlan Coin, Rony Swennen, Elizabeth A. B. Aitken

Subject: Biology And Life Sciences, Plant Sciences Keywords: Banana; fine mapping; quantitative trait locus; Musa acuminata ssp. malaccensis; Fusarium wilt; Fusarium oxysporum f. sp. cubense; Tropical Race 4; Subtropical Race 4; marker assisted selection; resistance gene expression; receptor-like kinase; RNAseq

Online: 5 May 2023 (09:51:49 CEST)

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Fusarium wilt of banana is a devastating disease that has decimated banana production worldwide. Host resistance to Fusarium oxysporum f. sp. cubense (Foc), the causal agent of this disease, is genetically dissected in this study using two Musa acuminata ssp. malaccensis segregating populations segregating for Foc Tropical (TR4) and Subtropical (STR4) race 4 resistance. Marker loci and trait association using 11 SNP-based PCR markers allowed the candidate region to be delimited to a 12.9 cM genetic interval corresponding to a 959 kb region on Chromosome 3 of ‘DH-Pahang’ reference assembly v4. Within this region, there is a cluster of pattern recognition receptors, namely leucine rich repeat ectodomain containing receptor-like protein kinases, cysteine-rich cell wall associated protein kinases, and leaf rust 10 disease-resistance locus receptor-like proteins positioned in an interspersed arrangement. Their transcript levels were rapidly upregulated in the resistant but not in susceptible F2 progenies at the onset of infection. This suggests that one or several of these genes may control resistance at this locus. To confirm the segregation of single-gene resistance, we generated an inter-cross between the resistant parent ‘Ma850’ and a susceptible line ‘Ma848’, to show that the STR4 resistance co-segregated with marker ‘28820’ at this locus. Finally, an informative SNP marker 29730 allowed the locus specific resistance to be assessed in a collection of diploid and polyploid banana plants. Out of the 60 lines screened, 22 lines were predicted to carry resistance at this locus, including lines known to be TR4 resistant, such as ‘Pahang’, ‘SH-3362’, ‘SH-3217’, ‘Ma-ITC0250’, and ‘DH-Pahang/CIRAD 930’. Additional screening in the International Institute for Tropical Agriculture’s collection suggests that the dominant allele is common among in the elite ‘Matooke’ NARITA hybrids, as well as in other triploid or tetraploid hybrids derived from East African highland bananas. Fine-mapping and candidate gene identification will allow characterization of molecular mechanisms underlying TR4 resistance. The markers developed in this study can now aid the marker-assisted selection of TR4 resistance in breeding programs around the world.

Preprint ARTICLE | doi:10.20944/preprints201905.0370.v1

Intraoperative Cryosurgical Freezing Assists with Local Drug Delivery and Targeting of Tumor Fluids in VX2 Model, which Translates in Locoregional Drug Targeting of Tumor Fluids during Cryoprobe-Assisted Surgical Resection of Breast Cancer

Patrick Le Pivert, Yueyong Xiao, Xiaofeng He, Olexeii Dronov, Nikolai Korpan

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Targeted drug delivery, VX2 tumor, breast cancer, cryoablation, cryo-assisted injection, cryo-assisted resection, blue dye, epirubicin.

Online: 30 May 2019 (13:44:41 CEST)

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Preprint REVIEW | doi:10.20944/preprints202402.0273.v1

Endoscopic Diagnosis of Small Bowel Tumor

Tomonori Yano, Hironori Yamamoto

Subject: Medicine And Pharmacology, Gastroenterology And Hepatology Keywords: balloon-assisted enteroscopy; capsule endoscopy; CT enterography

Online: 5 February 2024 (10:54:57 CET)

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Preprint BRIEF REPORT | doi:10.20944/preprints202311.0559.v1

The Contemporary Approach to Chylothorax— Single‐Center Experience with the Proposal of Management Algorithm

Ivan Bačić, Domagoj Morović, Ivan Kovačić, Dario Vukosav, Željko Čulina, Jakov Mihanovic

Subject: Medicine And Pharmacology, Surgery Keywords: Chylothorax; Thoracic Duct; Video-Assisted Thoracoscopic Surgery

Online: 8 November 2023 (14:41:32 CET)

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Preprint REVIEW | doi:10.20944/preprints202305.0365.v1

Recent Advances in the Plasma-Assisted Synthesis of Silicon-Based Thin-Films and Nanostructures

Pietro Mandracci, Paola Rivolo

Subject: Chemistry And Materials Science, Surfaces, Coatings And Films Keywords: silicon; thin-films; nanostructures; growth; plasma-assisted

Online: 5 May 2023 (11:41:55 CEST)

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Preprint REVIEW | doi:10.20944/preprints202204.0228.v1

High Throughput Phenotyping Approach

Anand Kumar, Prashant Kaushik

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: vegetables; high throughput phenotyping; genomic assisted breeding

Online: 26 April 2022 (06:00:45 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202101.0599.v1

Experiences of Women Undergoing Assisted Reproductive Technology in GhanA: A Qualitative Study

JUSTICE JONATHAN, Judith Anaman-Tagbor, Lily Asare, Bernice Osarfo, Rita Attivor, Afia Bonsu, Elizabeth Fialor, Elvis Tarkang

Subject: Social Sciences, Gender And Sexuality Studies Keywords: Assisted Reproductive Technologies; Ghana; Infertility; Pregnancy; Women

Online: 29 January 2021 (06:03:03 CET)

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Preprint ARTICLE | doi:10.20944/preprints202001.0021.v1

Robot-Assisted Therapy Influence on Kinematic and EMG Activity in the Upper Limb for Children with Cerebral Palsy

Sana Raouafi, Maxime Raison, Sofiane Achiche

Subject: Public Health And Healthcare, Physical Therapy, Sports Therapy And Rehabilitation Keywords: cerebral palsy; robot assisted therapy; EMG; kinematic

Online: 3 January 2020 (02:38:16 CET)

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Preprint ARTICLE | doi:10.20944/preprints202309.1280.v1

Rice (Oryza sativa L.) Grain Size, Shape, and Weight-Related QTLs Identified using GWAS with Multiple GAPIT Models and High-Density SNP Chip DNA Markers

Nkulu Rolly Kabange, Gamenyah Daniel Dzorkpe, Dong-Soo Park, Youngho Kwon, Sais-Beul Lee, So-Myeong Lee, Ju-Won Kang, Seong-Gyu Jang, Ki-Won Oh, Jong-Hee Lee

Subject: Biology And Life Sciences, Plant Sciences Keywords: SNP Chip DNA Marker; GAPIT; GWAS; Genomic Selection; Grain traits; Rice

Online: 20 September 2023 (02:17:56 CEST)

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This study investigated novel quantitative traits loci (QTLs) associated with the control of grain shape and size as well as grain weight in rice. We employed a joint strategy multiple GAPIT (Genome Association and Prediction Integrated Tool) models [(Bayesian-information and Linkage-disequilibrium Iteratively Nested Keyway (BLINK)), Fixed and random model Circulating Probability Uniform (FarmCPU), Settlement of MLM Under Progressive Exclusive Relationship (SUPER), and General Linear Model (GLM)]–High Density SNP Chip DNA Markers (60,461) to conduct a Genome-Wide Association Study (GWAS). GWAS was performed using genotype and grain-related phenotypes of 143 recombinant inbred lines (RILs). Data show that parental lines (Ilpum and Tung Tin Wan Hein 1, TTWH1, Oryza sativa L., ssp. japonica and indica, respectively) exhibited divergent phenotypes for all analyzed grain traits), which was reflected in their derived population. GWAS results revealed the association between seven SNP Chip makers and quantitative trait loci (QTLs) for grain length, co-detected by all GAPIT models on (Chr) 1–3, 5, 7, and 11), were qGL1-1BFSG (AX-95918134, Chr1: 3820526 bp) explains 65.2%–72.5% of the phenotypic variance explained (PVE). In addition, qGW1-1BFSG (AX-273945773, Chr1: 5623288 bp) for grain width explains 15.5%–18.9% of PVE. Furthermore, BLINK or FarmCPU identified three QTLs for grain thickness independently, and explain 74.9% (qGT1Blink, AX-279261704, Chr1: 18023142 bp) and 54.9% (qGT2-1Farm, AX-154787777, Chr2: AX-154787777 bp) of the observed PVE. For t length-to-width ratio, the qLWR2BFSG (AX-274833045, Chr2: 10000097 bp) explains nearly 15.2%–32% of PVE for LWR. Likewise, the major QTL for thousand-grain weight (TGW) was detected on Chr6 (qTGW6BFSG, AX-115737727, 28484619 bp) and explains 32.8%–54% of PVE. The qTGW6BFSG QTL coincides with qGW6-1Blink for grain width and explained 32.8%–54% of PVE. Putative Candidate genes pooled from major QTLs for each grain traits have interesting annotated functions that require functional studies to elucidate their function in the control of grain size, shape, or weight in rice. Genome selection analysis proposed makers useful for downstream marker-assisted selection based on genetic merit of RILs.

Preprint ARTICLE | doi:10.20944/preprints202309.0227.v1

Identification of Breast Cancer Metastasis Markers Using Machine Learning Approaches with Gene Expression Profiles

Jinmyung Jung, Sunyong Yoo

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: metastasis marker; gene expression; machine learning; XGBoost; breast cancer; feature importance.

Online: 5 September 2023 (05:21:26 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.1101.v1

Identification of Diagnostic Metabolic Signatures in Thyroid Tumors using Mass Spectrometry Imaging

Xinxin Mao, Luojiao Huang, Tiegang Li, Abliz Zeper, Jiuming He, Jie Chen

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: thyroid follicular adenoma; thyroid follicular carcinoma; mass spectrometry; tumor; diagnose; marker

Online: 15 June 2023 (08:13:52 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1440.v1

Development of an Accurate Polymerase Chain Reaction (PCR) Assay for Genetic Sex Identification in Lumpfish (Cyclopterus lumpus) based on Male-specific anti-Mullerian Hormone (amh) Gene

Kasidis Chaiyasut, Paul Merviel, Peter Palma, Johanna Perschthaler, Eduardo Jimenez Fernandez, Andrew Davie, Alejandro P. Gutierrez

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Anti-Müllerian hormone; Cyclopterus lumpus; Male-specific marker; Genetic sex identification

Online: 19 May 2023 (11:36:20 CEST)

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Preprint REVIEW | doi:10.20944/preprints202310.1442.v1

Glutathionyl Hemoglobin and Its Emerging Role as a Clinical Biomarker of Chronic Oxidative Stress

Andrea Scirè, Giulia Casari, Brenda Romaldi, Lidia De Bari, Cinzia Antognelli, Tatiana Armeni

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: glutathione; glutathionyl hemoglobin; PTMs; S-glutathionylation; glutathionylated hemoglobin; oxidative stress; clinical marker.

Online: 23 October 2023 (12:00:12 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202209.0301.v1

Genome-Wide Association Mapping of Resistance to Warrior (-) Yellow Rust Race at the Seedling Stage in Current Central and Northern European Winter Wheat Germplasm

Fahimeh Shahinnia, Volker Mohler, Lorenz Hartl

Subject: Biology And Life Sciences, Plant Sciences Keywords: Warrior (-) race; stripe rust; GWAS; Yr29/Lr46 gene; European wheat; SNP marker

Online: 20 September 2022 (09:58:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202012.0406.v1

Measurement of Circulating Tumor Cells Determines Treatment Response and Exitus in Patients with Hepatocellular Carcinoma Submitted to Transarterial Chemoembolization

Patricia de la Cruz-Ojeda, Elena Navarro-Villarán, María I. Gómez-Espejo, Sandra Dios-Barbeito, Inés Fernández-Luque, Concepción Meana, María Carmen Garrido-Navas, Manuel F Domínguez-Borrero, Juan M. Praena-Fernández, María J. Serrano, José M. Álamo-Martínez, Carlos M. Gutiérrez-Martín, Álvaro Giráldez-Gallego, Francisco J. Castell-Monsalve, María T. Ferrer-Ríos, Jordi Muntané

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Hepatocellular carcinoma; transcatheter arterial chemoembolization; circulating tumor cells; tumor progression; predictive marker

Online: 16 December 2020 (11:26:42 CET)

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Preprint ARTICLE | doi:10.20944/preprints202002.0202.v1

Forage Performance and Detection of Marker Trait Associations with Potential for Napier Grass (Cenchrus Purpureus) Improvement

Ermias Habte, Meki S. Muktar, Asebe Abdena, Jean Hanson, Alieu M. Sartie, Alemayehu Teressa Negawo, Juarez Machado, Franscisco ledo, Chris S. Jones

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Napier grass; elephant grass; EMBRAPA; forage yield; feed quality; marker trait association

Online: 15 February 2020 (15:01:37 CET)

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Preprint ARTICLE | doi:10.20944/preprints201910.0147.v1

Whole Genome Alignment Based Development of Molecular Marker for Detecting Leptosphaeria maculans and Leptosphaeria biglobosa, the Causal Agent of Blackleg Disease in Brassicas

Mostari Jahan Ferdous, Mohammad Rashed Hossain, Denison Michael Immanuel Jesse, Jong-In Park, Hee-Jeong Jung, Hoy-Taek Kim, Ill-Sup Nou

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: blackleg; brassica; diagnosis; effector; genome alignment; leptosphaeria biglobosa; leptosphaeria maculans; marker; PCR

Online: 13 October 2019 (16:08:55 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201809.0224.v1

A Distinct Pattern of Circulating Amino Acids Characterizes Older Persons with Physical Frailty and Sarcopenia: Preliminary Results from the BIOSPHERE Study

Riccardo Calvani, Anna Picca, Federico Marini, Alessandra Biancolillo, Jacopo Gervasoni, Silvia Persichilli, Aniello Primiano, Helio Coelho-Junior, Maurizio Bossola, Andrea Urbani, Francesco Landi, Roberto Bernabei, Emanuele Marzetti

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: aging; muscle; protein; metabolism; metabolomics; profiling; biomarkers; multi-marker; physical performance; multivariate

Online: 12 September 2018 (17:11:33 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201701.0088.v2

NANOG Plays a Hierarchical Role in the Transcription Network Regulating the Pluripotency and Plasticity of Adipose Tissue-Derived Stem Cells (ASCs)

Maria Pitrone, Giuseppe Pizzolanti, Laura Tomasello, Antonina Coppola, Lorenzo Morini, Gianni Pantuso, Romina Ficarella, Valentina Guarnotta, Sebastio Perrini, Francesco Giorgino, Carla Giordano

Subject: Biology And Life Sciences, Endocrinology And Metabolism Keywords: Adipose derived stem cell (ASC); Regenerative medicine; embryonic stem cell marker network

Online: 20 January 2017 (04:56:06 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.1878.v1

Is Chest Tube Omission Safe for Patients with Primary Spontaneous Pneumothorax Scheduled for VATS?

Chang Wan Kim, Il Hwan Park, Chun Sung Byun

Subject: Medicine And Pharmacology, Pulmonary And Respiratory Medicine Keywords: Pneumothorax; Chest tube; Video-assisted thoracoscopic surgery; Recurrence

Online: 26 January 2024 (09:41:43 CET)

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Preprint ARTICLE | doi:10.20944/preprints202310.0831.v1

The Impact of Transportation to the Cortisol Level of Dwarf Rabbits Bred to Animal Assisted Interventions

Éva Suba-Bokodi, István Nagy, Marcell Molnár

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: rabbits; animal assisted interventions; AAI; stress; transportation; cortisol

Online: 12 October 2023 (20:19:54 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202309.0997.v1

Assisting Observational Astronomy for Public with Smart Telescopes and eXplainable Artificial Intelligence

Olivier Parisot, Mahmoud Jaziri

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: eXplainable Artificial Intelligence; Electronically Assisted Astronomy; Smart Telescope

Online: 15 September 2023 (03:54:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202112.0341.v1

Investigation on Deformation Behavior in the Surface of Metal foil with Ultrasonic Vibration-Assisted Micro-Forging

ZiDong Yin, Ming Yang

Subject: Chemistry And Materials Science, Nanotechnology Keywords: microfoming; energy field assisted micro/nanoforming; ultrasonic vibration

Online: 21 December 2021 (13:53:15 CET)

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Preprint ARTICLE | doi:10.20944/preprints202109.0471.v1

Physical-Mechanical Behavior and Water-Barrier Properties of Biopolymers-Clay Nanocomposites

Heidy Lorena Calambas, Abril Fonseca, Dayana Adames, Yaneli Aguirre-Loredo, Carolina Caicedo

Subject: Chemistry And Materials Science, Polymers And Plastics Keywords: Biodegradable polymers; Nanocomposite; Ultrasound-assisted; Mechanical properties; Montmorillonite

Online: 28 September 2021 (12:27:17 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202007.0234.v1

Microwave Extraction vs. Other Techniques for Industrial Scale Cannabis Extraction

Marilena Radoiu, Harmandeep Kaur, Anna Bakowska-Barczak, Steven Splinter

Subject: Chemistry And Materials Science, Applied Chemistry Keywords: cannabis; THC; CBD; microwave assisted extraction; continuous flow

Online: 11 July 2020 (09:04:17 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201807.0232.v2

Separation of Negatively Charged TiO₂-Coated Polystyrene Beads in Microfluidic Device

Chalinee Phiphatanaphiphop, Komgrit Leksakul, Rungrueang Phatthanakun, Wutthikrai Busayaporn, Chatree Saiyasombat, Pat Phothongkam, Isa Anshori, MdMohosin Rana, Hiroaki Suzuki

Subject: Engineering, Electrical And Electronic Engineering Keywords: microfluidic; microelectrodes; negatively charged; TiO2; assisted reproductive technology

Online: 18 September 2018 (10:34:00 CEST)

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This research was presented the special designed microfluidic device generated for sperm separation based on assumption of different surface electrical charged of sperms X and Y. However, to avoid ethical problem, the microfluidic chip has been tested with the mimic electrical charged particles, TiO₂-coated Polystyrene beads, (TiO₂-coated Ps-beads), instead of spermatozoa. The work has been separated into three main parts. Firstly, the simply but efficient fabrication of negatively charged TiO₂-coated Ps-beads has been presented. In addition, various characterization techniques such as X-ray diffraction (XRD), Tungsten Scanning Electron Microscopy (W-SEM) with energy-dispersive X-ray spectroscopy (EDS) mode, and X-ray Absorption Spectroscopy (XAS), have been reported in this work to elucidate the reasons behind the persistence of negatively charged on the surface of TiO₂-coated Ps-beads. Results show that the fabricated TiO₂-coated Ps-beads was partly coated in the mixed forms of amorphous Ti⁴⁺ and had caused a negatively charge to appear on the surface after fabrication and had sustained its electrical charged for long. Secondly, process of simulation and fabrication of microfluidic device was presented. Finally the negatively charged TiO₂-coated Ps-beads were tested in this microfluidic devices. For design of microfluidic devices integrated with a couple of microelectrodes, the simulated structures were fabricated by photolithographic technique and tested with the Ps-beads. Percentage of validation for Ps-beads separation indicated that the 100 mm-distance-between-electrodes microfluidic device exhibits to be the highest performance prototype at 86.96%. For further confirmation, another model so called the single path prototype has been established. It is confirmed by 92.59% of validation for the utilization of the device. The successfully designed microfluidic devices can be examined with actual spermatozoa later. Furthermore, process to fabricate the negatively charged TiO₂-coated Ps-beads can be established as testified samples for development of other microfluidic devices.

Preprint ARTICLE | doi:10.20944/preprints202310.0254.v2

Evaluating Metabarcoding Markers for Identifying Zooplankton and Ichthyoplankton Communities to Species in the Salish Sea: Morphological Comparisons and Rare, Threatened or Invasive Species

Carol A. Stepien, Haila K. Schultz, Sean M McAllister, Emily L Norton, Julie E. Keister

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: copepods; genetic marker; ichthyoplankton; larvae; metabarcoding; MiSeq; mtDNA; Puget Sound; Salish Sea; zooplankton

Online: 15 November 2023 (10:01:49 CET)

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Preprint REVIEW | doi:10.20944/preprints202309.1485.v1

Biomarkers of Postpartum Depression: A Narrative Review

Stefan Modzelewski, Aleksandra Julia Oracz, Kamila Iłendo, Aleksandra Sokół, Napoleon Waszkiewicz

Subject: Medicine And Pharmacology, Psychiatry And Mental Health Keywords: postpartum depression, marker, biomarkers, peripartum, postpartum, cytokines, HPA, oxidative stress, genetics, nutrients, pathogenesis

Online: 22 September 2023 (05:13:21 CEST)

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Preprint REVIEW | doi:10.20944/preprints202201.0280.v1

Roles of Podoplanin in Malignant Progression of Tumor

Hiroyuki Suzuki, Mika K Kaneko, Yukinari Kato

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: podoplanin, PDPN, tumor malignancy, tumor marker, antibody therapy, cancer-specific monoclonal antibody, CasMab

Online: 19 January 2022 (16:05:50 CET)

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Preprint ARTICLE | doi:10.20944/preprints202107.0509.v1

Genetic Variation of Native Perilla Germplasms Collected from South Korea Using SSR Markers and Morphological Characteristics

Jun Seok Oh, Kyu Jin Sa, Hyeon Park, Do Yoon Hyun, Sookyeong Lee, Ju-Hee Rhee, Ju Kyong Lee

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Perilla crop; genetic resources; morphological traits; principal component analysis; SSR marker; genetic variation

Online: 22 July 2021 (08:04:28 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202005.0122.v1

Impaired Social Skills as a Key Component of Clinical Depression: Associations with Severity of Illness, Self-Esteem, Family Functional Health Satisfaction, and Personality Features

Pitima Kurimoto, Peeraphon Lueboonthavatchai, Michael Maes

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: social skill deficits; major depression; stress; state marker; introvert personality; family supporting satisfaction

Online: 7 May 2020 (12:37:06 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0065.v1

Sperm Incubation in Bww Medium Induces Capacitation- Related Changes in the Lizard Sceloporus torquatus

Uriel Ángel Sánchez-Rivera, Norma Berenice Cruz-Cano, Alfredo Medrano, Carmen Álvarez-Rodríguez, Martín Martínez-Torres

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: sperm capacitation; BWW medium; lizard; incubation; assisted reproduction techniques

Online: 2 April 2024 (02:26:07 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.1626.v1

Production Capacity Prediction and Optimization in the Glycerin Purification Process: A Simulation-Assisted Few-Shot Learning Approach

Tawesin Jitchaiyapoom, Chanin Panjapornpon, Santi Bardeeniz, Mohd Azlan Hussain

Subject: Engineering, Chemical Engineering Keywords: glycerin purification; few-shot learning; production optimization; simulation-assisted.

Online: 28 February 2024 (10:52:24 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.0049.v1

Rapid and High-Yield Recovery of Sodium Alginate from Undaria pinnatifida via Microwave-Assisted Extraction

Ja Hyun Lee, Jong-Min Lim, Hyeon-Bin Nam, Kang Hyun Lee, Hah Young Yoo, Chulhwan Park

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Microwave-assisted extraction; sodium alginate; response surface methodology; optimization

Online: 2 January 2024 (07:51:46 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.1615.v1

Ultrasound-Assisted, Catheter-Directed Thrombolysis for Acute Intermediate/High Risk Pulmonary Embolism: Design of the Multicenter USAT IH-PE Registry and Preliminary Results

Claudia Colombo, Nicolò Capsoni, Filippo Russo, Mario Iannaccone, Marianna Adamo, Giovanna Viola, Ilaria Emanuela Bossi, Luca Villanova, Chiara Tognola, Camilla Curci, Francesco Morelli, Rossella Guerrieri, Lucia Occhi, Giuliano Chizzola, Antonio Rampoldi, Francesco Musca, Giuseppe De Nittis, Mario Galli, Giacomo Boccuzzi, Daniele Savio, Davide Bernasconi, Luciana D’Angelo, Andrea Garascia, Alaide Chieffo, Matteo Montorfano, Fabrizio Oliva, Alice Sacco

Subject: Medicine And Pharmacology, Pulmonary And Respiratory Medicine Keywords: pulmonary embolism; ultrasound assisted; catheter-directed thrombolysis; pulmonary hypertension

Online: 21 December 2023 (06:19:38 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.0489.v1

Efficacy of Robot-Assisted Ureteroureterostomy in Patients with Complex the Ureteral Stricture after Ureteroscopic Lithotripsy

Shuzo Hamamoto, Kazumi Taguchi, Kengo Kawase, Rei Unno, Masahiko Isogai, Koei Torii, Shoichiro Iwatsuki, Toshiki Etani, Taku Naiki, Atsushi Okada, Takahiro Yasui

Subject: Medicine And Pharmacology, Urology And Nephrology Keywords: robot-assisted uretero-ureterostomy; ureteral stricture; ureteroscopic lithotripsy; microcalcification

Online: 7 November 2023 (16:18:04 CET)

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Preprint ARTICLE | doi:10.20944/preprints202309.0456.v1

The Preparation of MgO Self-Epitaxial Films for YBCO High-Temperature Coated Conductors

Fei Yu, Yan Xue, Chaowei Zhong, Jiayi Song, Qiong Nie, Xin Hou, Baolei Wang

Subject: Engineering, Electrical And Electronic Engineering Keywords: ion beam-assisted deposition; magnesium oxide; homogeneous epitaxy; fluorene

Online: 7 September 2023 (05:06:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.1952.v1

The Fertility Outcome of Addition of Assisted Oocyte Activation Into Spindle View-Assisted Intracytoplasmic Sperm Injection in Patients With Low Fertilization Rate

Wei-Che Lo, Chieh-Yu Lin, Chia-Jung Li, Chiung-Hui Hou, Lin-Hsuan Dai, Hsien-Ming Wu

Subject: Medicine And Pharmacology, Obstetrics And Gynaecology Keywords: Assisted oocyte activation; ICSI; polarization microscopy; fertilization failure; blastocyst

Online: 28 June 2023 (05:22:26 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0172.v1

How May Longer Console-Time Influence Outcomes After Robot Assisted Radical Prostatectomy (RARP)

Mahmoud Farzat, Mohamed Elsherif, Florian M. Wagenlehner

Subject: Medicine And Pharmacology, Urology And Nephrology Keywords: Prostate cancer; robot assisted radical prostatectomy (RARP); console-time.

Online: 4 May 2023 (03:36:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202108.0418.v1

CAST as a Potential Oncogene From Machine Searching in Gastric Cancer Infiltrated with Macrophage and Associated with Lgr5

Kuang-Tsu Yang, Chia-Jung Li, Renin Chang, Jui-Tzu Wang, Yih-Wen Tarng, Jin-Shuen Chen

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: CAST; Lgr5; WNT; Gastric cancer; Machine assisted searching; macrophage

Online: 20 August 2021 (14:00:27 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202401.0361.v1

Spatial Computational Hepatic Molecular Biomarker Reveals LSECs Role in Mid Lobular Liver Zonation Fibrosis in DILI and NASH Induced Liver Injury

Munish Puri

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: DILI; NASH; liver zonation marker; bridging fibrosis; spatial transcriptomics; liver injury; computational biomarker; LSEC

Online: 4 January 2024 (07:10:59 CET)

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Preprint ARTICLE | doi:10.20944/preprints202310.2097.v1

A Pilot Multisensory Approach for Emotional Eating: Pivoting from VR to a 2-D Telemedicine Intervention during the Pandemic

Clelia Malighetti, Ciara Kelly Schnitzer, Sophie Lou YorkWilliams, Luca Bernardelli, Cristin Runfola, Giuseppe Riva, Debra L. Safer

Subject: Medicine And Pharmacology, Psychiatry And Mental Health Keywords: emotional eating; eating disorder; binge-eating disorder; bulimia nervosa; virtual reality; treatment; somatic marker

Online: 1 November 2023 (03:53:18 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.1196.v1

Optical, Electrical, Structural and Thermo-Mechanical Properties of Undoped and Tungsten-Doped Vanadium Dioxide Thin Films

Chuen-Lin Tien, Chun-Yu Chiang, Ching-Chiun Wang, Shih-Chin Lin

Subject: Physical Sciences, Optics And Photonics Keywords: Vanadium dioxide; thin film; electron beam evaporation; ion-assisted deposition

Online: 18 April 2024 (08:11:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.0226.v1

The Synergy Between Methanol M100 and Plasma Assisted Ignition System PAI to Achieve Increasingly Leaner Mixtures in a Single-Cylinder Engine.

Federico Ricci, Francesco Mariani, Stefano Papi, Jacopo Zembi, Michele Battistoni, Carlo Nazareno Grimaldi

Subject: Engineering, Automotive Engineering Keywords: lean combustion; kinetic; methanol fuel; Plasma Assisted Ignition; SI engine

Online: 5 March 2024 (06:42:22 CET)

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Preprint BRIEF REPORT | doi:10.20944/preprints202401.0624.v1

Restorative Reproductive Medicine: An Emerging New Treatment Process and a Prerequisite to Assisted Reproductive Technology for Treatment of Infertility.

Phil C. Boyle, Agnes Toth, Linda ONeill, Craig J. Turczynski

Subject: Medicine And Pharmacology, Reproductive Medicine Keywords: infertility; in-vitro fertilization; assisted reproductive technology; restorative reproductive medicine

Online: 8 January 2024 (13:43:16 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.1469.v1

A New Microwave-assisted Protocol for Cellulose Extraction from Eucalyptus and Pine Tree Wood Waste

Silvia Vinhas, Mafalda Sarraguça, Tânia Moniz, Salette Reis, Maria Rangel

Subject: Chemistry And Materials Science, Paper, Wood And Textiles Keywords: microwave-assisted extraction; cellulose extraction; hemicellulose; lignin; wood wastes; sustainability

Online: 23 November 2023 (05:44:05 CET)

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Preprint ARTICLE | doi:10.20944/preprints202309.1346.v1

Robot-Assisted and Manual Cochlear Implantation: An Intra-Individual Study of Speech Recognition

Clémentine MAHEO, Antoine MARIE, Renato TORRES, Jerrid ARCHUTICK, Jean-Christophe LECLERE, Rémi MARIANOWSKI

Subject: Medicine And Pharmacology, Otolaryngology Keywords: electrode array insertion; robot-assisted implantation; hearing outcome; scala translocation

Online: 20 September 2023 (09:57:50 CEST)

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Preprint REVIEW | doi:10.20944/preprints202209.0040.v1

Computer-Assisted Preoperative Planning of Bone Fracture Fixation Surgery: A State-Of-The-Art Review

Jet Moolenaar, Nazli Tümer, Sara Checa

Subject: Medicine And Pharmacology, Orthopedics And Sports Medicine Keywords: Bone fracture fixation; Osteosynthesis; Preoperative planning; Computer-assisted; Virtual surgery

Online: 2 September 2022 (15:30:04 CEST)

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Background Bone fracture fixation surgery is one of the most commonly performed surgical procedures in the orthopedic field. However, fracture healing complications occur frequently, and the choice of the most optimal surgical approach often remains challenging. In the last years, computational tools have been developed with the aim to assist preoperative planning procedures of bone fracture fixation surgery. Objectives The aims of this review are (1) to provide a comprehensive overview of the state-of-the-art in computer-assisted preoperative planning of bone fracture fixation surgery, (2) to assess the clinical feasibility of the existing virtual planning approaches, and (3) to assess their clinical efficacy in terms of clinical outcomes as compared to conventional planning methods. Methods A literature search was performed in the MEDLINE-PubMed, Ovid-EMBASE, Ovid-EMCARE, Web of Science, and Cochrane libraries to identify articles reporting on the clinical use of computer-assisted preoperative planning of bone fracture fixation. Results 79 articles were included to provide an overview of the state-of-the art in virtual planning. While patient-specific geometrical model construction, virtual bone fracture reduction, and virtual fixation planning are routinely applied in virtual planning, biomechanical analysis is rarely included in the planning framework. 21 of the included studies were used to assess the feasibility and efficacy of computer-assisted planning methods. The reported total mean planning duration ranged from 22 to 258 minutes in different studies. Computer-assisted planning resulted in reduced operation time (Standardized Mean Difference (SMD): -2.19; 95% Confidence Interval (CI): -2.87, -1.50), less blood loss (SMD: -1.99; 95% CI: -2.75, -1.24), decreased frequency of fluoroscopy (SMD: -2.18; 95% CI: -2.74, -1.61), shortened fracture healing times (SMD:-0.51; 95% CI: -0.97, -0.05) and less postoperative complications (Risk Ratio (RR): 0.64, 95% CI: 0.46, 0.90). No significant differences were found in hospitalization duration. Some studies reported improvements in reduction quality and functional outcomes but these results were not pooled for meta-analysis, since the reported outcome measures were too heterogeneous. Conclusions Current computer-assisted planning approaches are feasible to be used in clinical practice and have been shown to improve clinical outcomes. Including biomechanical analysis into the framework has the potential to further improve clinical outcome.

Preprint REVIEW | doi:10.20944/preprints202111.0546.v1

Review on Chemical Graph Theory and Its Application in Computer-Assisted Structure Elucidation

Mehmet Aziz Yirik, Kumsal Ecem Colpan, Saskia Schmidt, Maria Sorokina, Christoph Steinbeck

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: chemical graph theory; computational chemistry; CASE; computer-assisted structure elucidation

Online: 29 November 2021 (15:35:43 CET)

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Preprint REVIEW | doi:10.20944/preprints202010.0454.v1

A Comprehensive Review of the Influence of Electric Field on Flame Characteristics

Yin Ma, Tong Li, Jun Yan, Xiaorong Wang, Ji Gao, Zuoyu Sun

Subject: Engineering, Automotive Engineering Keywords: Review; Electric Field; Ionic wind; Electric assisted combustion; Soot emission

Online: 22 October 2020 (09:54:38 CEST)

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221 articles found