Search | Preprints.org

Preprint ARTICLE | doi:10.20944/preprints202208.0151.v1

Cardiovascular Effects of the BNT162b2 mRNA COVID-19 Vaccine in Adolescents

Suyanee Mansanguan, Prakaykaew Charunwatthana, Watcharapong Piyaphanee, Wilanee Dechkhajorn, Akkapon Poolcharoen, Chayasin Mansanguan

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: BNT162b2 mRNA COVID-19 vaccine; COVID-19 vaccine; cardiovascular effects; myocarditis; adolescents; Thailand

Online: 8 August 2022 (10:40:23 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202307.1198.v1

Autopsy Proven Fatal COVID-19 Vaccine-Induced Myocarditis

Nicolas Hulscher, Roger Hodkinson, William Makis, Peter McCullough

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: myocarditis; sudden death; chest pain; autopsy; necropsy; COVID-19; COVID-19 vaccines; mRNA; SARS-CoV-2 vaccination; death; excess mortality; spike protein; organ system

Online: 18 July 2023 (09:34:51 CEST)

Show abstract| Download PDF| Share

Background: COVID-19 vaccines have been linked to myocarditis which in some circumstances can be fatal. This systematic review aims to investigate potential causal links between COVID-19 vaccines and death from myocarditis using post-mortem analysis. Methods: We performed a systematic review of all published autopsy reports involving COVID-19 vaccination-related myocarditis through July 3^rd, 2023. All autopsy studies that include COVID-19 vaccine-induced myocarditis as a possible cause of death were included, without imposing any additional restrictions. Causality in each case was determined by three independent reviewers with cardiac pathology experience and expertise. Results: We initially identified 1,691 studies and, after screening for our inclusion criteria, included 14 papers that contained 28 autopsy cases. The cardiovascular system was the only organ system affected in 26 cases. In 2 cases, myocarditis was characterized as a consequence from multisystem inflammatory syndrome (MIS). The mean and median number of days from last COVID-19 vaccination until death was 6.2 and 3 days, respectively. Most of the deaths occurred within a week from the last injection. We established that all 28 deaths were causally linked to COVID-19 vaccination by independent adjudication. Conclusions: The temporal relationship, internal and external consistency seen among cases in this review with known COVID-19 vaccine-induced myocarditis, its pathobiological mechanisms and related excess death, complemented with autopsy confirmation, independent adjudication, and application of the Bradford Hill criteria to the overall epidemiology of vaccine myocarditis, suggests there is a high likelihood of a causal link between COVID-19 vaccines and death from suspected myocarditis in cases where sudden, unexpected death has occurred in a vaccinated person. Urgent investigation is required for the purpose of risk stratification and mitigation in order to reduce the population occurrence of fatal COVID-19 vaccine-induced myocarditis.

Preprint HYPOTHESIS | doi:10.20944/preprints202303.0441.v1

IgG4 Antibodies Induced by mRNA Vaccines Generate Immune Tolerance to SARS-CoV-2’spike Protein by Suppressing the Immune System

Vladimir N. Uversky, Elrashdy M. Redwan, William Makis, Alberto Rubio-Casillas

Subject: Medicine And Pharmacology, Pulmonary And Respiratory Medicine Keywords: IgG4 antibodies; mRNA vaccines; immuno-tolerance; auto-immunity; SARS-CoV-2; COVID-19.

Online: 27 March 2023 (03:56:26 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints201810.0708.v1

Overview of Genomic Tools for Circular Visualization in next-Generation Genomic Sequencing Era

Alisha Parveen, Sukank Khurana, Abhishek Kumar

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: circular visualization; circos; genomics; next-generation sequencing

Online: 30 October 2018 (07:06:35 CET)

Show abstract| Download PDF| Share

Preprint SHORT NOTE | doi:10.20944/preprints202012.0493.v2

Future Considerations for the mRNA–Lipid Nanoparticle Vaccine Platform

Botond Igyártó, Sonya Jacobsen, Sonia Ndeupen

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: mRNA-LNP vaccines; side effects; dendritic cells

Online: 24 December 2020 (13:51:51 CET)

Show abstract| Download PDF| Share

Preprint COMMUNICATION | doi:10.20944/preprints202006.0044.v1

Major Concerns on the Identification of Bat Coronavirus Strain RaTG13 and Quality of Related Nature Paper

xiaoxu lin, Shizhong Chen

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Epidemiology; COVID-19; coronavirus; bat; RaTG13; BtCoV/4991; SARS-CoV-2; Pangolin Coronavirus; next generation sequencing

Online: 5 June 2020 (06:17:26 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201609.0056.v1

The Impacts of Different Green Manure on Soil Microbial Communities and Crop Health

Hongwu Yang, Jiaojiao Niu, Jiemeng Tao, Yabing Gu, Chao Zhang, Siyuan She, Wu Chen, Hongqi Yang, Huaqun Yin

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: green manure; soil microbial communities; crop health; Illumina sequencing

Online: 18 September 2016 (08:56:53 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202104.0034.v5

SARS-CoV2 Variants and Vaccines mRNA Spikes Fibonacci Numerical UA/CG Metastructures

Jean-Claude Perez

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: SARS-CoV2; Biomathematics; vaccine; variants; mRNA; Fibonacci; numerical standing waves

Online: 20 April 2021 (10:05:55 CEST)

Show abstract| Download PDF| Share

ABSTRACT. In this paper, we suggest a biomathematical numerical method for analysing mRNA nucleotides sequences based on UA/CG Fibonacci numbers proportions. This method is used to evaluate then compare the spike genes related to the main SARS-CoV2 VARIANTS currently circulating within the world population. The 10 main results proposed to be reproduced by peers are: 1/ SARS-CoV2 genome and spike evolution in one year 2020-2021. 2/ SARS-CoV2 Origins. 3/ Comparing 11 reference variants spikes. 4/ analysing 32 CAL.20C California variant patients spikes. 5/ Toward a meta mRNA Fibonacci gene end message code. 6/ Analysing S501 UK, S484 South Africa and « 2 mutations » INDIA variants. 7/ Suggesting a possible variants spike mRNA palindrome symmetry metastructure improving mRNA stability then infectiousness. 8/ Analysing Fibonacci Metastructures in the mRNA coding for the vaccines PFIZER and MODERNA. 9/ Does the CG-rich modification of the synonymous codons of the spikes of the 2 mRNA vaccines affect the expression and quantity of SARS-CoV2 antibodies? 10/ The exceptional case of the Brazilian variant P.1. Particularly, we suggest the following conjecture at mRNA folding level : CONJECTURE of SARS-CoV2 VARIANTS: The growth of long Fibonacci structures in the shape of "podiums" for almost all of the variants studied (UK, California, South Africa, India, etc.) suggests the probable folding of the Spike mRNA in the form of a "hairpin", which can strengthen the cohesion and the lifespan of this mRNA. Finally, we show that these kinds of Fibonacci matastructures disapear TOTALLY by analysing the published mRNA sequences of PFIZER and MODERNA vaccines. One fact is certain, the two mRNAs of the Moderna and Pfizer vaccines will result in a low functionality of the spike vaccine. This is because their designers by seeking greater stability, have doped to build CG rich sequences which, as soon as they are inserted into the human host, will, paradoxically, seek to mutate, like SARS-CoV2 variants, towards CG ==> UA forms in order to improve their STABILITY and LIFETIME. We conclude using new biomathematics theoretical methods (Master code and numerical standing waves), and comparing the Spikes of the two vaccines Moderna and Pfizer, that there will be very probable differences in stability and shelf life of the two respective mRNAs vaccines. However, “State of the Art” analyzes will disclose that their two protein sequences are strictly identical. By modified their synonymous codons using different strategies, no one can guarantee that the quantity of antibodies generated will be identical in the two cases. We wish to draw attention to the great ADAPTATION power - at the global scale of their genomes - of the most infectious VARIANTS, such as the BRAZIL 20J / 501Y.V3 variant (P.1). This is very worrying for the VACCINES <==> VARIANTS run: We demonstrate how the Brazilian variant P.1 which becomes uncontrollable in Brazil in April 2021 has a level of organization of long metastructures of 17,711 bases covering the genome which is 3.6 more important than that of the 2 reference genomes SARS-CoV2 and worldwide D614G. We suggest that this high level of overall structure of this variant contributes to the stability of this genome and, might explain its greater contagiousness.

Preprint REVIEW | doi:10.20944/preprints202005.0376.v1

Computational Strategies to Combat COVID-19: Useful Tools to Accelerate SARS-CoV-2 and Coronavirus Research

Franziska Hufsky, Kevin Lamkiewicz, Alexandre Almeida, Abdel Aouacheria, Cecilia Arighi, Alex Bateman, Jan Baumbach, Niko Beerenwinkel, Christian Brandt, Marco Cacciabue, Sara Chuguransky, Oliver Drechsel, Robert D. Finn, Adrian Fritz, Stephan Fuchs, Georges Hattab, Anne-Christin Hauschild, Dominik Heider, Marie Hoffmann, Martin Hölzer, Stefan Hoops, Lars Kaderali, Ioanna Kalvari, Max von Kleist, René Kmiecinski, Denise Kühnert, Gorka Lasso, Pieter Libin, Markus List, Hannah F. Löchel, Maria J. Martin, Roman Martin, Julian Matschinske, Alice C. McHardy, Pedro Mendes, Jaina Mistry, Vincent Navratil, Eric Nawrocki, Áine Niamh O'Toole, Nancy Palacios-Ontiveros, Anton I. Petrov, Guillermo Rangel-Piñeros, Nicole Redaschi, Susanne Reimering, Knut Reinert, Alejandro Reyes, Lorna Richardson, David L. Robertson, Sepideh Sadegh, Joshua B. Singer, Kristof Theys, Chris Upton, Marius Welzel, Lowri Williams, Manja Marz

Subject: Biology And Life Sciences, Virology Keywords: virus bioinformatics; SARS-CoV-2; sequencing; epidemiology; drug design; tools

Online: 23 May 2020 (11:01:11 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201608.0179.v1

A Prelimary Molecular Approach for Characterizing Microorganisms Having the Potentials for Bioleaching of Iron Ore

Emmanuel Ekpa, Ikechukwu Onwurah, Lawrence Ezeanyika, John Odiba

Subject: Chemistry And Materials Science, Biomaterials Keywords: bioleaching; molecuar analysis; bacteria; sequencing; iron ore

Online: 19 August 2016 (09:23:04 CEST)

Show abstract| Download PDF| Share

One consequence of the global technological advancement in conventional metallurgy is the fast depletion rate of valuable minerals, which are also becoming increasingly difficult to find in pure and economically viable forms. This has spurred more interest in technologies that investigate ability of different microorganisms to mobilize valuable metals from their ores via diverse metabolic processes. This study was carried out therefore to isolate, identify and characterize iron solubilizing bacteria from Iron stones of Agbaja iron ore mining site of Kogi State, Nigeria. Crushed samples in the range of 0.25µm and 0.75µm particle sizes were cultured in a modified 9k media to facilitate bacterial growth and pure cultures were then isolated and sub-cultured for further bioleaching studies. Morphological and biochemical analysis suggests that some of the bacteria identified are members of Acidithiobacillus spp, Pseudomonas spp, and Leptospirillum spp. Studies conducted on pure cultures and mixed consortium of the identified organisms shows that a mixture of the three organisms leached iron ore to about 96.16%. Also results of growth pattern due to bacteria countafter 24-72hours of incubation ranged between 0.1×10³ cfu/ml and 12.3 ×10³ cfu/ml for Acidithiobacillus spp. The need to explore the molecular characteristics of these organisms with a view to generating more information on the quality/quantity of their DNA for future cloning activities was also investigated in this work. DNA was extracted using zymo fungal/bacterial extraction mini prep kit ^TM (cat #6001) and subsequently subjected to 1% agarose gel electrophoresis. Visible bands were obtained with Alpha Innotech Gel Documentation Machine. DNA amplification was carried out using a pettier based thermo cycler PCR machine and electrophoresed on 1.5% agarose gel. Results of the PCR shows a visible band corresponding to 1.5kbp using this primer 27F (5¹-GAGTTTGATCCTGGCTCAG-3¹) and 1492R (5^1-GGTTACCTTGTTACGACT-3¹). DNA purity check shows two of the bacteria possess very good qualities for sequencing for further molecular analysis.

Preprint ARTICLE | doi:10.20944/preprints201609.0080.v1

Determination of Genetic Variations of Toll-like Receptor (TLR) 2, 4 and 6 with Next Generation Sequencing in Native Cattle Breeds of Anatolia and Holstein Friesian

Nuket Bilgen, Bengi Cinar Kul, Victoria Offord, Dirk Werling, Okan Ertugrul

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Anatolian Black; East Anatolian Red; South Anatolian Red; Turkish Grey; Holstein Friesian; Innate immunity; Next Generation Sequencing; TLR2; TLR4; TLR6

Online: 23 September 2016 (05:44:43 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint CASE REPORT | doi:10.20944/preprints202206.0308.v2

A Case Report: Multifocal Necrotizing Encephalitis and Myocarditis after BNT162b2 mRNA Vaccination against Covid-19

Michael Mörz

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: mRNA Vaccine; autopsy

Online: 25 August 2022 (03:54:58 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202002.0385.v1

Rapid Design of a Bait Capture Platform for Culture- and Amplification-Free Next-Generation Sequencing of SARS-CoV-2

Jalees A. Nasir, David J. Speicher, Robert A. Kozak, Hendrik N. Poinar, Matthew S. Miller, Andrew G. McArthur

Subject: Biology And Life Sciences, Virology Keywords: Coronavirus; SARS-CoV-2; enrichment; next-generation sequencing

Online: 26 February 2020 (02:27:21 CET)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints201801.0109.v1

What is Developmental Dyslexia?

John Stein

Subject: Social Sciences, Psychology Keywords: dyslexia; reading; magnocellular neurons; vision; hearing; phonology; sequencing; timing; temporal processing; transient; coloured filters; rhythm; music; omega 3s

Online: 12 January 2018 (07:15:33 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201609.0024.v1

Identification of One BOCR Mutation and Five NF1 Mutations in Male Patients with Neurofibromatosis Type 1 and Congenital Pseudarthrosis of the Tibia

Zhifan Zhou, Yong Zeng, Xinchun Wu, Fei Li, Xiaoyan Liu, Lu Zhou, Lihua Hou, Silong Sun, Lu Shen, Zhangyuan Lin

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: neurofibromatosis type 1; congenital pseudarthrosis of the tibia; whole-exome sequencing; targeted sequencing; BCOR

Online: 7 September 2016 (11:19:00 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint CASE REPORT | doi:10.20944/preprints202209.0051.v1

A Case Report: Acute Myocardial Infarction, Coronal Arteritis and Myocarditis after BNT162b2 mRNA Vaccination against Covid-19

Michael Mörz

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: mRNA Vaccine; autopsy

Online: 5 September 2022 (08:14:56 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202001.0332.v1

Fish Transcriptomics: Applied to Our Understanding of Aquaculture

Joseph Heras

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: transcriptomics; aquaculture; genetics; next generation sequencing

Online: 28 January 2020 (05:10:55 CET)

Show abstract| Download PDF| Share

New challenges arise in the face of global climate change which impact every ecosystem on earth, including aquatic systems. This is evident in observations made in regard to the world’s oceans, which show trends of incremental changes in ocean surface temperatures, sea levels, and ocean acidity. These environmental shifts impact human resources such as fisheries and aquaculture. In addition, according to the World Bank, the increase in human population will also require more food and nutrient production, which include industries such as aquaculture. With this increasing demand in aquaculture and fisheries, we must develop efficient and productive methods to operate these industries. We can use genetic methods, specifically transcriptomic information to better understand the biology of our source of nutrition. With the advent of RNASeq techniques, we can provide a better understanding about growth and development, immune function and stress, and adaptations. The use of population genetics or (genomics) to detect Single Nucleotide Polymorphisms (SNPs) between populations or closely related species can provide greater insight from stock structure to fishery-induced evolution. In addition, candidate loci can be investigated further to better understanding evolutionary processes, which provide clues on physiological adaptations and gene expression patterns that can help elucidate how these organisms respond to their current environment. In addition, the use of transcriptomic analyses such as differential gene expression can be used to determine resilience in various environmental conditions such as pollution, hypoxic/anoxic conditions, fluctuations in salinity, and temperature extremes. There has been an increase in transcriptomic studies for many aquaculture species, which has aimed at improving our understanding of growth, development, and metabolism, providing vital information for fisheries and aquaculture industries to make adjustments to environmental conditions such as oxygen availability, nutrition, and salinity. All of these aspects provide insightful information for advancing our knowledge of aquaculture, fisheries and conservation management.

Preprint REVIEW | doi:10.20944/preprints201905.0130.v1

Fast Neutron Mutagenesis in Plants: Advances, Applicability and Challenges

Surbhi Kumawat, Nitika Rana, Ruchi Bansal, Gautam Vishwakarma, Sayaji Mehetre, Bikram Kishore Das, Manish Kumar, Satish Yadav, Humira Sonah, Tilak Raj Sharma, Rupesh Deshmukh

Subject: Biology And Life Sciences, Plant Sciences Keywords: induced mutagenesis; fast neutron irradiation; mutation mapping; deletagene; next-generation sequencing technologies; reverse genetics

Online: 10 May 2019 (14:38:21 CEST)

Show abstract| Download PDF| Share

Working Paper REVIEW

Rhizosphere Microbiome Modulators: Contributions of Nitrogen Fixing Bacteria towards Sustainable Agriculture

Nicholas Ozede Igiehon, Olubukola Oluranti Babalola

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: microbiomes; next generation sequencing, plant yield; rhizobacteria; rhizosphere; sustainable agriculture

Online: 25 January 2018 (17:41:19 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201703.0216.v1

A Dysbiotic Mycobiome Dominated by Candida albicans is Identified within Oral Squamous Cell Carcinomas

Manosha Perera, Nezar Noor Al-hebshi, Irosha Perera, Deepak Ipe, Glen Ulett, David J. Speicher, Tsute Chen, Newell W. Johnson

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: 18S rRNA; fungi; mycobiome; carcinoma; High-Throughput Nucleotide Sequencing; microbiome; mouth; squamous cell

Online: 30 March 2017 (05:37:37 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints201703.0024.v1

Inflammatory Bacteriome Featuring Fusobacterium nucleatum and Pseudomonas aeruginosa Identified in Association with Oral Squamous Cell Carcinoma

Nezar Noor Al-hebshi, Akram Thabet Nasher, Mohamed Yousef Maryoud, Husham E. Homeida, Tsute Chen, Ali Mohamed Idris, Newell W. Johnson

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: 16S rRNA; bacteria; bacteriome; carcinoma; High-Throughput Nucleotide Sequencing; microbiome; mouth; squamous cell

Online: 6 March 2017 (04:47:15 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints201703.0081.v1

Deciphering microRNAs and Their Associated Hairpin Precursors in a Non-Model Plant, Abelmoschus esculentus

Kavitha Velayudha Vimala Kumar, Nagesh Srikakulam, Priyavathi Padbhanabhan, Gopal Pandi

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: miRNAs; pre-miRNAs sequencing; Abelmoschus esculentus; Next generation sequencing; non-model plant

Online: 14 March 2017 (10:15:14 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint REVIEW | doi:10.20944/preprints202106.0725.v1

Antigen Presentation of mRNA Based and Viral Vector Corona Vaccines

Ger T. Rijkers, Nynke Weterings, Andres Obregon-Henao, Tara Dutt, Tara Dutt, Frans van Overveld, Marcela Henao-Tamayo

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: mRNA vaccine; viral vector vaccine; Spike protein; antigen presentation; polyethylene glycol; platelet factor 4; thrombosis

Online: 30 June 2021 (09:46:15 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202009.0207.v1

SMARTdenovo: A de novo Assembler Using Long Noisy Reads

Hailin Liu, Shigang Wu, Alun Li, Jue Ruan

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: SMARTdenovo; single-molecule sequencing; genome assembly

Online: 9 September 2020 (10:48:24 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202402.1267.v1

Inadvertent Exposure to Pharmacologically Designed Lipid Nanoparticles Via Bodily Fluids: Biologic Plausibility and Potential Consequences

Matthew Halma, Jessica Rose, Peter McCullough

Subject: Medicine And Pharmacology, Transplantation Keywords: Covid-19 vaccination; mRNA vaccine; lipid nanoparticles; blood transfusion; breastfeeding exposure; pharmacovigilance

Online: 22 February 2024 (04:00:44 CET)

Show abstract| Download PDF| Share

Exposure to vaccine lipid nanoparticles, mRNA, adenoviral DNA, and or Spike protein from one of the approved Covid-19 vaccines, or through secondary exposure, as through blood transfusion, is a potential source of harm. Blood reactions are an acknowledged side-effect of Covid-19 vaccination, not limited to hemolysis, paroxysmal nocturnal hemoglobinuria, chronic cold agglutinin disease, immune thrombocytopenia, haemophagocytosis, hemophagocytic lymphohistiocytosis, and many other blood related conditions. The observation of adverse events has motivated investigation into the cardiovascular mechanisms of harm by Covid-19 vaccines, and the biodistribution of vaccine contents. Biodistribution may not be limited to the body of the vaccine recipient, as a growing body of evidence demonstrates the possibility of secondary exposure to vaccine particles. These can be via bodily fluids and include the following routes of exposure: blood transfusion, organ transplantation, breastfeeding, and possibly other means. As covid-19 vaccines are associated with an increased risk of stroke, the persistence of vaccine artifacts in the blood presents a possible threat to a recipient of a blood donation from a vaccinated donor who suffered from vaccine induced thrombosis or thrombocytopenia. (VITT) We assess the feasibility and significance of these risks through an overview of the case report literature of blood disorders in vaccinated individuals, pharmacovigilance reports from the US Vaccine Adverse Events Reporting System (VAERS) and a meta-analysis of the available literature on organ transplants from vaccinated organ donors. Our analysis establishes biological mechanistic plausibility, a coherent safety signal in pharmacovigilance databases for secondary vaccine contents exposure (for the cases of blood transfusion and breastfeeding) and also an elevated level of adverse events in organ transplants from VITT-deceased donors, echoing increases in organ transplantation related complications seen in national statistics for some countries. Secondary exposure to vaccine artifacts is a potential explanation for some of the cases put forth, and requires a deeper investigation.

Working Paper REVIEW

Sequencing Enabling Design and Learning in Synthetic Biology

Pierre-Aurelien Gilliot, Thomas E. Gorochowski

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: sequencing; omics; synthetic biology; systems biology; machine learning; biological design

Online: 23 April 2020 (03:47:02 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201708.0103.v1

Cancer Etiology: Variation in Cancer Risk among Tissues is Poorly Explained by the Number of Gene Mutations

Miguel Lopez-Lazaro

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: stem cells; somatic mutations; cancer prevention; carcinogenesis; whole genome sequencing; stem cell division theory of cancer; bad luck of cancer

Online: 30 August 2017 (12:14:52 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201902.0020.v1

Moraxella nonliquefaciens and M. osloensis are Important Moraxella Species that Cause Ocular Infections

Samantha LaCroce, Mollie N. Wilson, John E. Romanowski, Jeffrey D. Newman, Vishal Jhanji, Robert M.Q. Shanks, Regis P. Kowalski

Subject: Medicine And Pharmacology, Ophthalmology Keywords: Moraxella; keratitis; conjunctivitis; endophthalmitis; DNA sequencing; MALDI TOF MS; Biolog ID system

Online: 6 February 2019 (06:27:07 CET)

Show abstract| Download PDF| Share

Preprint SHORT NOTE | doi:10.20944/preprints202008.0205.v3

The Anomalous Nature of the Fecal Swab Data, Receptor Binding Domain and Other Questions in RaTG13 Genome

Monali Rahalkar, Rahul Bahulikar

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: RaTG13; SARS-COV-2; Illumina sequencing, amplicon sequencing, NGS; fecal swab

Online: 5 October 2020 (12:20:17 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201805.0375.v1

Diversity of Gut Microbiota in Autism Reveals Differential Abundance of Prevotella and Akkermansia Species

Swati Agarwala, Bindushree Naik, Nallur B Ramachandra

Subject: Social Sciences, Behavior Sciences Keywords: autism phenome; gut microbiome; behaviour reversal; meta-analysis; 16srRNA sequencing; operational taxonomic units (OTUs)

Online: 25 May 2018 (16:15:27 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint REVIEW | doi:10.20944/preprints202101.0471.v1

Fluorescent Aptamers for Detection and Treatment of Pathogenic Bacteria and Cancer

Aarushi Sharma, Grace Ramena

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: aptamer; aptasensor; diagnosis; imaging; sequencing; therapeutics; probes; fluorescence; pathogenic bacteria; cancer cells; monoclonal antibodies; SELEX; nucleic acids

Online: 25 January 2021 (10:18:16 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202009.0483.v1

Enhancing the Landscape of Colorectal Cancer Using Targeted Deep Sequencing

Chul Seung Lee, In Hye Song, Ahwon Lee, Jun Kang, Yoon Suk Lee, In Kyu Lee, Young Soo Song, Sung Hak Lee

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: next-generation sequencing; colorectal cancer; ATM mutation; FBXW7 mutation

Online: 20 September 2020 (15:20:52 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint REVIEW | doi:10.20944/preprints202309.1385.v1

Clinical Rationale for Dietary Lutein Supplementation in Post COVID-19 and mRNA Vaccine Injury Syndromes

Anthony M Kyriakopoulos, Greg Nigh, Peter A McCullough, Stephanie Seneff

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Lutein; post-COVID syndrome; mRNA vaccination injury syndrome; inflammatory response; reactive oxygen species; reactive nitrogen species; arrhythmogenic heart phenotype; antioxidant defense; bioavailability; extra virgin olive oil

Online: 20 September 2023 (10:07:58 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202103.0103.v1

Automated Capture-Based NGS Workflow: One Thousand Patients Experience in a Clinical Routine Framework

Elena Tenedini, Fabio Celestini, Pierluigi Iapicca, Marco Marino, Sara Castellano, Lucia Artuso, Fiammetta Biagiarelli, Laura Cortesi, Angela Toss, Elena Barbieri, Luca Roncucci, Monica Pedroni, Rossella Manfredini, Mario Luppi, Tommaso Trenti, Enrico Tagliafico

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Next Generation Sequencing; Laboratory automation; Hereditary Cancer; Genetic Testing; Clinical Genomics.

Online: 2 March 2021 (16:00:24 CET)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints201709.0163.v1

From Genotype to Phenotype: A Clinical Perspective for Hospital-Acquired Pneumonia

Etienne Ruppé, Abdessalam Cherkaoui, Vladimir Lazarevic, Stéphane Emonet, Jacques Schrenzel

Subject: Medicine And Pharmacology, Pulmonary And Respiratory Medicine Keywords: next-generation sequencing; whole-genome sequencing; hospital-acquired pneumonia; antibiotic resistance; prediction; clinical metagenomics

Online: 30 September 2017 (04:49:23 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201705.0206.v1

Genome Characterization of a Novel Binary Toxin-Positive Strain of Clostridium difficile and Comparison with the Epidemic 027 and 078 Strains

Zhong Peng, Sidi Liu, Xiujuan Meng, Wan Liang, Biao Tang, Yuanguo Wang, Juping Duan, Chenchao Fu, Bin Wu, Anhua Wu, Chunhui Li

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Clostridium difficile; ST201; binary toxin-positive; whole genome sequencing; comparative genomic analysis

Online: 30 May 2017 (06:15:11 CEST)

Show abstract| Download PDF| Share

A novel binary toxin-positive non-027, non-078 Clostridium difficile strain designated LC693 whose sequence type was ST201 was isolated from the fecal sample of a patient with severe diarrhea in China. To understand the pathogenesis basis of C. difficile ST201, this recently recovered isolate LC693 was then chosen for whole genome sequencing. The project finally generated an estimated genome size of approximately 4.07 Mbp. The genome sequence was then analyzed together with the other two ST201 strains VL-0104 and VL-0391 and compared to the epidemic 027/ST1 and 078/ST11 strains. Phylogenetic analysis demonstrated that the ST201 strains belonged to clade 3. Genome size of the three ST201 strains ranged from 4.07 Mb~4.16 Mb, with an average GC content between 28.5%~28.9%. The ST201 genomes contained more than 40 antibiotic resistance genes and 15 of them were predicted to be associated with vancomycin-resistance, suggesting that they may have a strong antibiotic resistance. The ST201 strains contained a typical clade 3 specific PaLoc with a Tn6218 element inserted, and those genes harbored on their PaLoc that participated in the toxin expression and regulation were highly homologues to the epidemic 027 and 078 strains, with the exception of tcdC. A truncated TcdC was found in the ST201 strains, which is suggestive to have a contribution to the toxin production of the ST201 strains. In addition, the ST201 strains contained intact binary toxin coding and regulation genes, which is also proposed to contribute to the virulence. Genome comparison of the ST201 strains with the epidemic 027 and 078 strain identified 641 genes specific for the C. difficile ST201, and a number of them were predicted as fitness and virulence associated genes. The identification of those genes also contributes to the pathogenesis of the ST201 strain. To our knowledge, this is the first study that the genome sequence of C. difficile ST201 was discussed in detail, and the present study would have a contribution to understanding the pathogenesis basis of C. difficile ST201.

Preprint REVIEW | doi:10.20944/preprints202302.0142.v1

Recent Advances in the Lipid Nanoparticle-Mediated Delivery of mRNA Vaccines

K Swetha, Niranjan G Kotla, Lakshmi Tunki, Arya Jayaraj, Suresh K. Bhargava, Haitao Hu, Srinivasa Reddy Bonam, Rajendra Kurapati

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: vaccine; mRNA; COVID 19; lipid nanoparticles

Online: 8 February 2023 (09:55:30 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201808.0423.v1

mitoMaker: A Pipeline for Automatic Assembly and Annotation of Animal Mitochondria Using Raw NGS Data

Alex Schomaker-Bastos, Francisco Prosdocimi

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: mitochondrial DNA; mitochondrial genome; genome assembly; genome annotation; next generation sequencing; animal genomics; partial genomics; bioinformatics

Online: 24 August 2018 (03:24:37 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints201905.0207.v1

Construction of a Single-Nucleotide Polymorphism Marker Based Quantitative Trait Loci Map and Validation of Resistance Loci to Bacterial Wilt (Ralstonia solanacearum) in Tomato

Il Sheob Shin, Jung Ching Hsu, Shu Mei Huang, Jaw Rong Chen, Jaw Fen Wang, Peter Hanson, Roland Schafleitner

Subject: Biology And Life Sciences, Horticulture Keywords: bacterial wilt; Ralstonia solanacearum; genotype-by-sequencing; disease resistance; quantitative trait loci; Solanum lycopersicum

Online: 16 May 2019 (10:35:18 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Bacterial wilt (BW), caused by Ralstonia solanacearum is one of the major biotic factors limiting tomato production in the humid tropics. Pyramiding of resistance genes through marker-assisted selection is an efficient way to develop durable BW resistant cultivars. Tomato line ‘Hawaii 7996’ (H7996) is a stable and robust resistance source against various R. solanacearum strains. Major BW resistance quantitative trait loci (QTLs) Bwr-12 and Bwr-6, and several minor or strain specific QTLs have been coarse-mapped in this line, but none has been fine-mapped and validated. The objective of the current study was to construct a high density genetic map using single-nucleotide polymorphism (SNP) markers derived from genotyping-by-sequencing, fine-map Bwr-12 and Bwr-6 and determine the effects of these QTLs using a near isogenic line (NIL) population. A high density genetic map using 1,604 SNP markers with an average distance of 0.82 cM was developed for 188 F9 recombinant inbred lines derived from the cross H7996 × WVa700. A total of seven QTLs associated with BW resistance to race 1-phylotype I or/and race 3-phylotype II strains were located on chromosomes 6 (Bwr-6.1, 6.2, 6.3 and 6.4) and 12 (Bwr-12.1, Bwr-12.2 and Bwr-12.3) with logarithm of odds (LOD) scores of 6.2-15.6 and 6.2-31.1, explaining 14.2-33.4% and 15.9-53.9% of the total phenotypic variation contributed from H7996, respectively. To validate the genetic effects of the two QTL regions, a set of 80 BC3F3 NILs containing different sections of Bwr-6 with or without Bwr-12 was phenotyped for disease severity after challenge with either race 1-phylotype I Pss4 or race 3-phylotype II Pss1632 BW strains over two seasons. Bwr-6.1 specific to Pss4 and Bwr-6.3 specific to Pss1632 were mapped to an interval of 5.0 cM (P < 0.05) between 6_33,444,000_SLM6-47 and 6_33,868,000_SLM6-124 SNP marker, and to 2.7 cM (P < 0.01) between positions 6_35,949,000 _SLM6-107 to 6_36,750,000_SLM6-82 marker, respectively. In addition, the specific effect of Bwr-12 for resistance to Pss4 (LOD score of 5.8-16.1, P < 0.01) was confirmed and markers for this QTL have already been made available previously.

Preprint REVIEW | doi:10.20944/preprints201807.0071.v1

The Role of Next-Generation Sequencing in Precision Medicine: A Review of Outcomes in Oncology

Margaret Morash, Hannah Mitchell, Himisha Beltran, Olivier Elemento, Jyotishman Pathak

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: precision medicine; next generation sequencing; oncology, patient outcomes; health insurance coverage

Online: 4 July 2018 (11:06:43 CEST)

Show abstract| Download PDF| Share

Preprint CONCEPT PAPER | doi:10.20944/preprints202009.0723.v1

Analyzing the Distribution of Mutations for Glycogen Storage Disease Type 1a in Turkey and Suggested Gene Therapy Methods for Its Treatment

Aysun Mantas, Cemre Ucaryilmaz, Busra Seniz Demir, Fatma Nur Guler, Sezgin Mengi, Cemre Can Inci, Ozum Kilic, Hale Ahsen Babar, Gulay Ceylaner, Serdar Ceylaner, Cihan Tastan

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Glycogen Storage Disease Type 1a, Glucose-6-phosphatase Catalytic Subunit (G6PC), Glucose-6-phosphatase (G6Pase), prime editing, mRNA delivery, CRISPR

Online: 30 September 2020 (08:05:17 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201810.0382.v1

Linkages of Soil Nutrients and Diazotrophic Microbiome under Sugarcane-Legume Intercropping

Manoj Kumar Solanki, Chang-Ning Li, Fei-Yong Wang, Zhen Wang, Tao-Ju Lan, Rajesh Kumar Singh, Pratiksha Singh, Li-Tao Yang, Yang-Rui Li

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: intercropping; microbial community; high throughput sequencing; nifH gene; sugarcane

Online: 17 October 2018 (10:20:19 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint REVIEW | doi:10.20944/preprints202301.0323.v1

An Introduction to Nanopore Sequencing: Past, Present, and Future Considerations

Morgan MacKenzie, Christos Argyropoulos

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: next-generation sequencing; nanopore sequencing; biosensors; single-molecule analysis; molecular diagnostics; genetics; transcriptomics; epigenetics

Online: 18 January 2023 (07:36:43 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202008.0718.v1

Clinically Relevant Germline Mutations in a Cohort of Young Women with Breast Cancer: A Comprehensive Analysis of Hereditary-Cancer Genes from Whole-Exome Sequencing

Liliana Gomez-Flores-Ramos, Micheal Dean, Kristine Jones, Mingyi Wang, Cynthia Villarreal-Garza, Rosa María Álvarez-Gómez, Talia Wegman-Ostrosky, Nancy Reynoso-Noverón, Verónica Fragoso-Ontiveros, Luisa Sánchez-Zamorano, Miguel Trujillo-Martínez, Lizbeth Grimaldo, Patricia Gallegos-Arreola, Patricia Ostrosky-Wegman, Alejandro Mohar-Betancourt

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: early-onset breast cancer; hereditary cancer; whole-exome sequencing; young women

Online: 31 August 2020 (09:46:26 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints201805.0244.v1

Evolutionary History of the GH3 Family Based on 48 Sequenced Plants and Identification of Jasmonic Acid-Relate GH3 Genes in Solanum tuberosum

Chao Zhang, Leilei Zhang, Dongdong Wang, Haoli Ma, Bailin Liu, Zheng Shi, Xiaohui Ma, Yue Chen, Qin Chen

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: GH3 family; sequencing plants; potato; jasmonic acids; tissues; biotic

Online: 17 May 2018 (12:01:00 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint REVIEW | doi:10.20944/preprints202101.0116.v1

Computational Methods for Chromosome-Scale Haplotype Reconstruction

Shilpa Garg

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Bioinformatics; Graphs; Medical genetics; sequencing methods

Online: 6 January 2021 (12:06:39 CET)

Show abstract| Download PDF| Share

Working Paper ARTICLE

The INDIA Mutations and B.1.617 Variants: Is there a Global "Strategy" for Mutations and Evolution of Variants of the SARS-CoV2 Genome?

Jean-claude Perez

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: SARS-CoV2; Biomathematics; vaccine; variants; mRNA; Fibonacci; Indian variants; B.1.617; B.1.617.2.

Online: 18 May 2021 (14:05:33 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201803.0149.v1

Adaptive Mutations in Influenza A/California/07/2009 Enhance Polymerase Activity and Infectious Virion Production

Patrick D. Slaine, Cara MacRae, Mariel Kleer, Emily Lamoureux, Sarah McAlpine, Michelle Warhuus, André M. Comeau, Craig McCormick, Todd Hatchette, Denys A. Khaperskyy

Subject: Biology And Life Sciences, Virology Keywords: influenza; H1N1; mouse adaptation; deep sequencing; polymerase; PA; PB1; defective viral genomes

Online: 19 March 2018 (08:59:26 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202206.0192.v1

Implementation of the eCDC/WHO Recommendation for Molecular Diagnosis of SARS-CoV-2 Omicron Subvariants and Its Challenges

Sin Hang Lee

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: eCDC; WHO; Sanger sequencing; Omicron variant; minor subvariants; BA.4/BA.5; BA.2; mul-ti-allelic; SNPs; recombinant

Online: 14 June 2022 (04:30:41 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202309.0495.v3

From Genes to Genomes: Opportunities, Challenges, and a Roadmap for Synteny-based Phylogenomics

Jacob Steenwyk, Nicole King

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: phylogenomics; synteny; chromosomes; long-read sequencing; tree of life; orthology; phylogenetic trees; incongruence

Online: 9 February 2024 (09:15:51 CET)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202106.0377.v2

A Review of SARS-CoV-2, Responsible for COVID-19: History, Biology, Infection Mechanism, Antigenic Vaccines, Their Risks and how an Alternate RBD Vaccine is Safer?

Z.M.G. Sarwar Jahangir, Arleta Helena Marnik

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: aa = amino acids; ACE-2 = receptor angiotensin-converting enzyme 2; cDNA = complementary DNA; mRNA = messenger RNA; orf = open reading frame; RBD = receptor binding protein; S-protein = Spike protein; SARS-CoV-2 = severe respiratory syndrome coronavirus 2; Vaccines.

Online: 22 June 2021 (11:53:34 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint REVIEW | doi:10.20944/preprints201811.0571.v2

Revisiting Prostate Cancer in India: A Genomic View

Prameesha Perera, Ayam Gupta, Nidhi Shukla, Mamta Nehra, Mukesh Sharma, Sneha Mishra, Vikram Singh Chauhan, Maneesh Kumar Vijay, Suresh Kumar Jatawa, Archana Tiwari, Krishna Mohan Medicherla, Praveen Mathur, Devendra Sharma, Prashanth Suravajhala

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: prostate cancer; prostate-specific antigen; incidence; genomics; next generation sequencing

Online: 3 April 2019 (10:15:50 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201803.0271.v1

Blackcurrant Leaf Chlorosis Associated Virus: Next-Generation Sequencing Reveals an Extraordinary Virus with Multiple Genomic Components Including Evidence of Circular RNA

Delano James, James Phelan, Daniel Sanderson

Subject: Biology And Life Sciences, Plant Sciences Keywords: Idaeovirus; Blackcurrant leaf chlorosis associated virus; next-generation sequencing (NGS); bridge reads; abutting primers; RNase R digestion; circular RNA; concatenated RNA

Online: 30 March 2018 (11:40:46 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201912.0093.v1

Genome-Wide Association Study for Resistance to the Meloidogyne javanica Causing Root-Knot Nematode in Soybean

Jean Carlos Alekcevetch, André Luiz de Lima Passianotto, Everton Geraldo Capote Ferreira, Adriana Brombini dos Santos, Danielle Cristina Gregório da Silva, Waldir Pereira Dias, François Belzile, Ricardo Vilela Abdelnoor, Francismar Corrêa Marcelino-Guimarães

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: mixed linear model; genotyping-by-sequencing; functional validation; RT-qPCR; resistance genes; GWAS

Online: 7 December 2019 (12:41:39 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints201805.0379.v1

Substrate-Induced Response in Biogas Process Performance and Microbial Community Relates Back to Inoculum Source

Tong Liu, Li Sun, Åke Nordberg, Anna Schnürer

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: anaerobic digestion; Co-digestion; CSTR; BMP-test; Illumina sequencing; T-RFLP; glycoside hydrolase families 5 and 48

Online: 27 May 2018 (12:53:51 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202301.0133.v2

The Novelty of mRNA Vaccines and Potential Harms: A Scoping Review

Matthew Halma, Jessica Rose, Theresa Lawrie

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: COVID-19 vaccination; mRNA vaccines; Clinical Trials; Safety Assessment; Novel Technologies; Spike protein

Online: 12 January 2023 (03:16:56 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202009.0097.v1

Genetic Diversity and Population Structure in a Regional Collection of Kersting’s Groundnut (Macrotyloma geocarpum (Harms) Maréchal & Baudet)

Konoutan M. Kafoutchoni, Eric E. Agoyi, Symphorien Agbahoungba, Achille E. Assogbadjo, Clément Agbangla

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: orphan crop; genotyping-by-sequencing; inbreeding; pre-breeding; population genetics; DArTseq; isolation by distance

Online: 4 September 2020 (11:11:37 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint TECHNICAL NOTE | doi:10.20944/preprints202102.0410.v2

A Modified High-Throughput Screening Protocol to Isolate Bacteriophages from Environmental Samples

Abdallah Abdelsattar, Rana Nofal, Salsabil Makky, Amera El-Sayed, Ayman El-Shibiny

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Phage Purification; phage isolation; phage characterization; jumbo bacteriophages; top agar; double agar overlay; high throughput sequencing

Online: 19 February 2021 (09:53:32 CET)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202102.0230.v1

Astrocytes in Alzheimer´s Disease: Pathological Significance and Molecular Pathways

Pranav Preman, Maria Alfonso-Triguero, Elena Alberdi, Alexej Verkhratsky, Amaia M Arranz

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Astrocyte, Alzheimer´s disease, neurodegeneration, transcriptomics, RNA sequencing (RNA-seq), cellular states.

Online: 9 February 2021 (10:04:24 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201811.0395.v1

Rapid Diagnosis of Capnocytophaga canimorsus Septic Shock in an Immunocompetent Individual Using Real-Time Nanopore Sequencing

Seweryn Bialasiewicz, Tania P.S. Duarte, Son H. Nguyen, Vichitra Sukumaran, Alexandra Stewart, Sally Appleton, Miranda E. Pitt, Arnold Bainomugisa, Amy V. Jennison, Rikki Graham, Lachlan J.M. Coin, Krispin Hajkowicz

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: nanopore sequencing; droplet digital PCR; Capnocytophaga canimorsus; diagnosis; sepsis

Online: 16 November 2018 (09:45:38 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202201.0390.v1

Analytic and clinical validation of a pan-cancer NGS liquid biopsy test for the detection of copy number amplifications, fusions and exon skipping variants.

Audrey Audetat, Cherie Tschida, Sarah Kreston, Adam Stephen, Brittany D'Alessio, Brittany D’Alessio, Leisa Jackson, John Williamson, Hestia Mellert, Niki Givens, Ubaradka G. Sathyanarayana, Gary A. Pestano

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: non-small cell lung cancer (NSCLC); liquid biopsy; cell-free nucleic acid (cfNA); next generation sequencing (NGS); CNA (copy number amplifications); fusions, exon-skipping.

Online: 26 January 2022 (03:21:12 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202204.0091.v1

Evidence-Based Evaluation of PCR Diagnostics for SARS-Cov-2 and The Omicron Variants by Sanger Sequencing

Sin Hang Lee

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: SARS-CoV-2; SARS-CoV; RT-PCR; Sanger sequencing; RT-qPCR; receptor-binding domain (RBD); N-terminal domain (NTD); Omicron; multi-allelic SNPs; false-positive

Online: 12 April 2022 (03:57:29 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints201901.0081.v1

IRES Trans-Acting Factors, Key Actors of the Stress Response

Anne-Claire Godet, Florian David, Fransky Hantelys, Florence Tatin, Eric Lacazette, Barbara Garmy-Susini, Anne-Catherine Prats

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: gene regulation; translation; mRNA; IRES; ITAF; hnRNP; chaperone; stress; nucleocytoplasmic translocation; ribosome; lncRNA; translation initiation factor; P-bodies

Online: 9 January 2019 (08:49:45 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202002.0192.v1

Microbial Patterns in Rumen Are Associated with Gain of Weight in Beef Cattle

Anderson Freitas, Diego David, Beatriz Takagaki, Luiz Fernando Wurdig Roesch

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: bacteria; fungi; livestock; microbiome; next generation sequencing

Online: 14 February 2020 (10:32:54 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202010.0426.v1

Molecular Identification of Fusarium Spp. Isolated From Wheat Based on Sequencing of Internal Transcribed Spacer (ITS) Region

Deyana Gencheva, Georgi Beev

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Fusarium spp.; Triticum aestivum; Internal Transcribed Spacer region (rDNA-ITS); DNA sequencing

Online: 21 October 2020 (10:27:26 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201910.0156.v1

Complete Genomic Sequence of Noni mosaic virus (NoMV), a Novel Potyvirus Associated with a Mosaic Disease in Morinda citrifolia L.

Nai-Tong Yu, Zhi-Ying Cai, Zhongguo Xiong, Yan Yang, Zhi-Xin Liu

Subject: Biology And Life Sciences, Virology Keywords: noni mosaic virus; potyvirus; complete genome; morinda citrifolia l; illumina transcriptomic sequencing

Online: 14 October 2019 (09:29:57 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint REVIEW | doi:10.20944/preprints202301.0460.v1

COVID-19 mRNA Vaccines: From Inflammation to Hyperinflammation

Girolamo Giannotta, Antonio Murrone, Nicola Giannotta

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: COVID-19 mRNA vaccines; Myo-pericarditis and COVID-19 mRNA vaccines; Multisystem-Inflammatory-Syndrome and COVID-19 mRNA vaccines; arrhythmias and COVID-19 mRNA vaccines; Pathogenesis of myocarditis following COVID-19 mRNA vaccines; MIS-A; MIS-C; MIS-V; Myocarditis; COVID-19 mRNA vaccine Adverse Events.

Online: 26 January 2023 (02:50:29 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201807.0142.v1

Single-Molecule Dynamics and Discrimination between Hydrophilic and Hydrophobic Amino Acids in Peptides, through Controllable, Stepwise Translocation across Nanopores

Alina Asandei, Isabela S. Dragomir, Giovanni Di Muccio, Mauro Chinappi, Yoonkyung Park, Tudor Luchian

Subject: Chemistry And Materials Science, Nanotechnology Keywords: nanopore; peptide sensing; electrophysiology; single-molecule sequencing

Online: 9 July 2018 (13:26:06 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202008.0677.v1

Comparison of Illumina Versus Nanopore 16S rRNA Gene Sequencing of the Human Nasal Microbiota

Astrid. P. Heikema, Deborah Horst-Kreft, Stefan A. Boers, Rick Jansen, Saskia D. Hiltemann, Willem de Koning, Robert Kraaij, Maria A. J. de Ridder, Chantal B. van Houten, Louis J. Bont, Andrew P. Stubbs, John P. Hays

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Nasal microbiota; Illumina sequencing; nanopore sequencing; 16S rRNA gene; Bacterial species; Corynebacterium

Online: 30 August 2020 (16:04:50 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202208.0338.v1

Newcastle Disease Genotype VII Outbreaks in Poultry across Egypt are associated with Enzootic Prevalence of the Virus in Wild Birds

Amal A.M. Eid, Ashraf Hussein, Ola Hassanin, Reham M. Elbakrey, Rebecca Daines, Jean-Remy Sadeyen, Hanan M.F Abdien, Klaudia Chrzastek, Munir Iqbal

Subject: Biology And Life Sciences, Virology Keywords: migratory birds; Newcastle disease virus-GVII; poultry; phylogenetics; sequence-independent; sin-gle-primer amplification (SISPA); velogenic; whole genome sequencing (WGS)

Online: 18 August 2022 (10:40:10 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202109.0322.v1

Grapevine Virology in the Third-Generation Sequencing Era: From Virus Detection to Viral Epitranscriptomics

Vahid Jalali Javaran, Peter Moffett, Pierre Lemoyne, Dong Xu, Charith-Raj Adkar Purushothama, Mamadou L. Fall

Subject: Biology And Life Sciences, Virology Keywords: Grapevine; Viral Disease; Diagnostic Methods; RNA Sequencing; Nanopore Sequencing Technology; RNA modifications

Online: 20 September 2021 (10:43:01 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202107.0275.v1

Sequencing and Chromosome-Scale Assembly of Plant Genomes, Brassica rapa as a Use Case

Benjamin Istace, Caroline Belser, Cyril Falentin, Karine Labadie, Franz Boideau, Gwenaëlle Deniot, Loeiz Maillet, Corinne Cruaud, Laurie Bertrand, Anne-Marie Chèvre, Patrick Wincker, Mathieu Rousseau-Gueutin, Jean-Marc Aury

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Genome sequencing; de novo Assembly; Scaffolding; Chromosome-scale; Nanopore sequencing; Long reads; Optical maps; Bionano Genomics; Hi-C; Omni-C; Pore-C; Plant genomes

Online: 12 July 2021 (22:55:37 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint REVIEW | doi:10.20944/preprints201806.0036.v1

Molecular Aspects of Varicella-Zoster Virus Latency

Daniel P. Depledge, Tomohiko Sadaoka, Werner J. D. Ouwendijk

Subject: Biology And Life Sciences, Virology Keywords: varicella-zoster virus; latency; reactivation; sensory ganglia; VZV latency-associated transcript; open reading frame 63; RNA-sequencing; epigenetics; immunity

Online: 4 June 2018 (11:49:09 CEST)

Show abstract| Download PDF| Share

Working Paper REVIEW

Phage Assisted Continuous Evolution (PACE): A How-to Guide for Directed Evolution

Serban C. Popa, Ichiro Inamoto, Benjamin W. Thuronyi, Jumi A. Shin

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: continuous evolution; protein design; protein engineering; phage; bacterial one-hybrid; plaque assay; mutational analysis; DNA sequencing

Online: 5 November 2019 (02:19:01 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201809.0406.v1

RNA Sequence Analysis Reveals Expected and Novel Immuno-Modulatory Activities by Sutherlandia frutescens

Wei Lei, Yuan Lu, Jie Hou, Chen Chen, Jimmy D. Browning, Dennis B. Lubahn, Jianlin Cheng, William R. Folk, Grace Y. Sun, Kevin L. Fritsche

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: Sutherlandia frutescens, RNA sequencing, inflammation, TNF

Online: 20 September 2018 (10:16:04 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201802.0090.v1

High-throughput Sequencing Analysis of the Actinobacterial Apatial Diversity in Moonmilk Deposits

Marta Maciejewska, Magdalena Calusinska, Luc Cornet, Delphine Adam, Igor Pessi, Sandrine Malchair, Philippe Delfosse, Denis Baurain, Hazel Barton, Monique Carnol, Sébastien Rigali

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: antibiotics; geomicrobiology; Illumina sequencing; microbiome diversity; Streptomyces; Cave microbiology

Online: 12 February 2018 (16:30:42 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202003.0119.v1

Mining and Characterizing the SSR Markers for Black Rice Using the Illumina Sequencing Platform

Dhananjay Kumar, Prasanta Kumar Das, Chandra Singha, Bidyut Kumar Sarmah

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: black rice; transcriptome sequencing; genic SSRs; genetic diversity

Online: 7 March 2020 (09:03:34 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202007.0395.v1

Comparative Transcriptome Analysis of Two Contrasting Kiwifruit (Actinidia) Genotypes under Waterlogging Stress

Zhi Li, Yunpeng Zhong, Danfeng Bai, Muhammad Abid, Yongjie Zhang, Miaomiao Lin, Xiujuan Qi, Chungen Hu, Jinbao Fang

Subject: Biology And Life Sciences, Horticulture Keywords: Actinidia; waterlogging; RNA-sequencing; transcriptional adjustment

Online: 17 July 2020 (15:40:11 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202201.0372.v1

Non-Viral Gene Therapy Vectors for Cancer Treatment

Shrey Kanvinde, Tanmay Kulkarni, Suyash Deodhar, Deep Bhattacharya, Aneesha Dasgupta

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: gene therapy; non-viral vectors; gene delivery; cancer; nucleic acid delivery; nanoparticles; lipids; lipid nanoparticles; mRNA; siRNA

Online: 25 January 2022 (09:01:41 CET)

Show abstract| Download PDF| Share

Preprint COMMUNICATION | doi:10.20944/preprints202004.0253.v1

Optimizing Open Data to Support One Health: Best Practices to Ensure Interoperability of Genomic Data from Microbial Pathogens

Ruth E. Timme, William J. Wolfgang, Maria Balkey, Sai Laxmi Gubbala Venkata, Robyn Randolph, Marc Allard, Errol Strain

Subject: Biology And Life Sciences, Virology Keywords: Genomic Epidemiology; GenomeTrakr; microbial pathogen surveillance, NCBI submission; whole genome sequencing; QA/QC; One Health

Online: 16 April 2020 (05:26:42 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint REVIEW | doi:10.20944/preprints202201.0073.v1

Hospital-based RNA Therapeutics

Tulsi Damase, Roman Sukhovershin, Min Zhang, Daniel Kiss, John Cooke

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Messenger RNA • Hospital-based mRNA therapeutics • circular mRNA • self-amplifying mRNA • RNA-based CAR T-cell • RNA-based gene-editing tools

Online: 6 January 2022 (11:20:59 CET)

Show abstract| Download PDF| Share

Working Paper REVIEW

The Reuse of Public Datasets in the Life Sciences: Potential Risks and Rewards

Katharina Sielemann, Alenka Hafner, Boas Pucker

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: data science; reuse; sequencing data; genomics; bioinformatics; databases; computational biology; open science

Online: 16 July 2020 (12:39:43 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint REVIEW | doi:10.20944/preprints202011.0163.v1

Sanger Sequencing as the Holy Grail of Sequencing Technology

Ayobami Adesiyan, Ayomikun Kade, Kafayat Oladimeji, Kehinde Sowunmi

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Sanger Sequencing; DNA Sequencing; Chain Termination Sequencing

Online: 3 November 2020 (14:41:11 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201909.0170.v1

ARSA-16S: A New and Conceptually Different Approach for 16S-based Taxonomic Profiling

Eduardo Pareja-Tobes, Raquel Tobes

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: 16S analysis, metagenomics, amplicons, bioinformatics, DNA sequencing

Online: 16 September 2019 (16:41:43 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202012.0738.v1

Utility of Whole-Exome Sequencing in Pediatric and Medical Diagnosis

Mohd Fareed, Varun Sharma, Hemender Singh

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Next generation sequencing; Genetic disorders; Genomic medicine; Genetic counseling; Rare diseases

Online: 29 December 2020 (16:47:01 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201910.0154.v1

Whole Genome Sequencing of Familial Non-Medullary Thyroid Cancer Identifies Germline Alterations in MAPK/ERK and PI3K/AKT Signaling Pathways

Aayushi Srivastava, Abhishek Kumar, Sara Giangiobbe, Elena Bonora, Kari Hemminki, Asta Försti, Obul Reddy Bandapalli

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: papillary thyroid cancer; germline mutations; whole genome sequencing; predisposition markers; pathway analysis

Online: 13 October 2019 (17:07:34 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202007.0218.v1

Temporal and Cultivar-Specific Effects on Potato Root- and Rhizosphere Fungal Diversity

Kaire Loit, Liina Soonvald, Alar Astover, Eve Runno-Paurson, Maarja Öpik, Leho Tedersoo

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Solanum tuberosum; agroecosystems; high-throughput sequencing; fungal guild, fungal diversity; host specificity; potato cultivars

Online: 11 July 2020 (02:02:34 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints201807.0397.v2

Genome-Wide Association Study and Selection Signatures Detect Genomic Regions Associated with Seed Yield and Oil Quality in Flax

Frank You, Jin Xiao, Pingchuan Li, ZHEN YAO, Gaofeng Jia, Liqiang He, Santosh Kumar, Braulio Soto-Cerda, Scott Duguid, Helen Booker, Khalid Rashid, Sylvie Cloutier

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: flax; genome-wide association study (GWAS); selective sweep; genotyping by sequencing (GBS); bi-parental population; single nucleotide polymorphism (SNP); seed yield; plant height; maturity; fatty acid composition

Online: 3 August 2018 (15:34:24 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202012.0252.v1

Unforgettable: Optogenetic Stimulation of Prelimbic Pyramidal Neurons Maintains Fear Memories by Modulating Amygdala Pyramidal Neuron Transcriptome

Daniela Laricchiuta, Giuseppe Sciamanna, Juliette Gimenez, Andrea Termine, Carlo Fabrizio, Silvia Caioli, Francesca Balsamo, Anna Panuccio, Marco De Bardi, Luana Saba, Noemi Passarello, Debora Cutuli, Anna Mattioni, Cristina Zona, Valerio Orlando, Laura Petrosini

Subject: Social Sciences, Psychology Keywords: Fear extinction; Fear Conditioning; Medial Prefrontal Cortex; RNA sequencing; Differential Gene Expression; Electrophysiological Recordings; Excitatory Post-Synaptic Currents; Spinogenesis; Fear-related Disorders

Online: 10 December 2020 (11:38:50 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints201809.0378.v1

Characterization of Gene Families Encoding Beta-Lactamases of Gram-Negative Rods Isolated from Ready-to-Eat Vegetables in Mexico City

Rosalino Vázquez-López, Sandra Solano-Gálvez, Bertha Aliacia León-Chávez, María del Rocío Thompson-Bonilla, Tayde Guerrero-González, Eduardo Gómez-Conde, Daniel Martínez-Fong, Juan-Antonio González-Barrios

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: enterobacteriaceae; antibiotics; beta-lactamases; beta-lactam resistome; whole genome sequencing

Online: 19 September 2018 (09:47:42 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202010.0437.v1

The Important Role of Breast Microbiota in Breast Cancer

Kar-Yan Su, Wai-Leng Lee, Vinod Balasubramaniam

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: breast cancer; microbiota; bacteria; dysbiosis; pro-carcinogenic; anti-carcinogenic; genetics; next-generation sequencing; cancer treatments; cancer prevention

Online: 21 October 2020 (12:51:23 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201902.0172.v4

Protein-Encoding RNA-to-RNA Information Transfer in Mammalian Cells: Principles of RNA-Dependent mRNA Amplification

Vladimir Volloch

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: RNA-dependent amplification of mammalian mRNA; physiologically occurring intracellular PCR, iPCR; RNA-dependent RNA polymerase, RdRp; chimeric RNA; sense-strand RNA; antisense-strand RNA

Online: 12 June 2019 (12:21:59 CEST)

Show abstract| Download PDF| Share

The transfer of protein-encoding genetic information from DNA to RNA to protein, a process formalized as the “Central Dogma of Molecular Biology”, has undergone a significant evolution since its inception. It was amended to account for the information flow from RNA to DNA, the reverse transcription, and for the information transfer from RNA to RNA, the RNA-dependent RNA synthesis. These processes, both potentially leading to protein production, were initially described only in viral systems, and although RNA-dependent RNA polymerase activity was shown to be present, and RNA-dependent RNA synthesisfound to occur, in mammalian cells, its function was presumed to be restricted to regulatory. However, recent results, obtained with multiple mRNA species in several mammalian systems, strongly indicate the occurrence of protein-encoding RNA to RNA information transfer in mammalian cells. It can result in the rapid production of the extraordinary quantities of specific proteins as was seen in cases of terminal cellular differentiation and during cellular deposition of extracellular matrix molecules. A malfunction of this process may be involved in pathologies associated either with the deficiency of a protein normally produced by this mechanism or with the abnormal abundanceof a protein or of its C-terminal fragment. It seems to be responsible for some types of familial thalassemia and may underlie the overproduction of beta amyloid in sporadic Alzheimer’s disease. The aim of the present article is to systematize the current knowledge and understanding of this pathway. The outlined framework introduces unexpected features of the mRNA amplification such as its ability to generate polypeptides non-contiguously encoded in the genome, its second Tier, a physiologically occurring intracellular polymerase chain reaction, iPCR, a Two-Tier Paradox and RNA Dark Matter. RNA-dependent mRNA amplification represents a new mode of genomic protein-encoding information transfer in mammalian cells. Its potential physiological impact is substantial, it appears relevant to multiple pathologies and its understanding opens new venues of therapeutic interference, it suggests powerful novel bioengineering approaches and its further rigorous investigations are highly warranted.

Preprint SHORT NOTE | doi:10.20944/preprints201911.0318.v4

Single Molecule Protein Sequencing Based on the Superspecificity of tRNA Synthetases

G Sampath

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: protein sequencing; single molecule; nanopore; tRNA; amino acyl tRNA synthetase; codon; optical tag

Online: 18 February 2020 (11:50:29 CET)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202009.0162.v2

Chaos, Order and Systematics in Evolution of the Genetic Code

Lei Lei, Zachary F. Burton

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: amyloids; frozen accident; genetic code; hydrogels; liquid-liquid phase separation; mRNA; polyglycine; rRNA; ribosomes; translational fidelity; tRNA

Online: 21 October 2020 (10:48:18 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202107.0548.v1

Transfer Learning for the Detection and Diagnosis of Types of Pneumonia Including Pneumonia Induced by the COVID-19 from Chest X-Ray Images

Yusuf Brima, Marcellin Atemkeng, Stive Tankio Djiokap, Jaures Ebiele, Franklin Tchakounté

Subject: Engineering, Automotive Engineering Keywords: ANN; COVID-19; CT; mRNA; MRI; RT-PCR; SARS-CoV-2; XCR

Online: 23 July 2021 (15:02:40 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202111.0170.v3

A Deep Dive into Genome Assemblies of Non-vertebrate Animals

Nadège Guiglielmoni, Ramón Rivera-Vicéns, Romain Koszul, Jean-François Flot

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: genome assembly; sequencing; non-vertebrate animals

Online: 5 May 2022 (10:38:09 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202107.0335.v1

The Ever-Expanding Pseudomonas Genus: Description of 43 New Species and Partition of the Pseudomonas Putida Group

Léa Girard, Cédric Lood, Monica Höfte, Peter Vandamme, Hassan Rokni-Zadeh, Vera van Noort, Rob Lavigne, René De Mot

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Pseudomonadaceae; long-read sequencing; hybrid assembly; Taxonomy; Phylogeny; Non-Ribosomal Peptide Synthetases (NRPS); Cyclic Lipopeptides (CLPs).

Online: 14 July 2021 (13:11:32 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint REVIEW | doi:10.20944/preprints202011.0501.v1

Genome Sequencing Guide: An Introductory Toolbox to Whole-Genome Analysis Methods

Alexis Burian, Wufan Zhao, Te-Wen Lo, Deborah Thurtle-Schmidt

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: genomics; sequencing; ethics; bioinformatics

Online: 19 November 2020 (10:41:35 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint REVIEW | doi:10.20944/preprints202106.0574.v1

Viruses and Type 1 Diabetes: From Enteroviruses to the Virome

Sonia R Isaacs, Dylan B Foskett, Anna J Maxwell, Emily J Ward, Clare L Faulkner, Jessica Y X Luo, William D Rawlinson, Maria E Craig, Ki Wook Kim

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: enterovirus; type 1 diabetes; virome; vaccine; antiviral; islet autoimmunity; coxsackievirus; next-generation sequencing; unbiased sequencing

Online: 23 June 2021 (11:19:33 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202201.0225.v2

Misdiagnosis of Bacterial Pathogens by the Diagnostic Centers: A Potential Route for Antibiotic Resistance

Shanaz Fatema Bristy, Md Reaz Uddin, Bushra Ayat Meghla, Taslima Akter Tisha, Md. Aftab Uddin, Mohammad Tarequl Islam, Masayasu Mie, Eiry Kobatake, Mohib Ullah Khondoker, Taslin Jahan Mou, Md. Ahsanul Haq, Md. Fokhrul Islam, Zinia Islam, Sharif Hossain, Nafisa Azmuda, Mohd. Raeed Jamiruddin, Mainul Haque, Nihad Adnan

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Misdiagnosis; Random Amplification of Polymorphic DNA; 16S rRNA Sequencing; Loop-Mediated Isothermal Amplification; Antibiotic Resistance

Online: 19 January 2022 (11:39:41 CET)

Show abstract| Download PDF| Share

Search Results

447 articles found