Search | Preprints.org

Preprint BRIEF REPORT | doi:10.20944/preprints202002.0424.v1

Insufficient Sensitivity of RNA Dependent RNA Polymerase Gene of SARS-CoV-2 Viral Genome as Confirmatory Test using Korean COVID-19 Cases

Soyoun Kim, Dong-Min Kim, Baeckseung Lee

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: SARS-CoV-2; Korean COVID-19; Wuhan Corona virus; real time PCR Ct Value; Sensitivity; False Negative

Online: 28 February 2020 (12:03:13 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint REVIEW | doi:10.20944/preprints202004.0155.v1

COVID-19 Testing: Challenges, Limitations and Suggestions for Improvement

Emily Hu

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: COVID-19; Coronavirus; False-negative; Nucleic Acid Test; Screening; Diagnostic Accuracy; Missed Diagnosis; Epidemic; Infectious Disease

Online: 9 April 2020 (14:37:56 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202104.0481.v1

Minimizing Errors in RT-PCR Detection and Quantification of SARS-CoV-2 RNA for Wastewater Surveillance

Warish Ahmed, Stuart Simpson, Paul Bertsch, Kyle Bibby, Aaron Bivins, Linda Blackall, Silvia Bofill-Mas, Albert Bosch, Joao Brandao, Phil Choi, Mark Ciesielski, Erica Donner, Nishita D'Souza, Andreas Farnleitner, Daniel Gerrity, Raul Gonzalez, John Griffith, Pradip Gyawali, Charles Haas, Kerry Hamilton, Chanditha Hapuarachchi, Valerie Harwood, Rehnuma Haque, Greg Jackson, Stuart Khan, Wesaal Khan, Masaaki Kitajima, Asja Korajkic, Giuseppina La Rosa, Blythe Layton, Erin Lipp, Sandra McLellan, Brian McMinn, Gertjan Medema, Suzanne Metcalfe, Wim Meijer, Jochen Mueller, Heather Murphy, Colleen Naughton, Rachel Noble, Sudhi Payyappat, Susan Petterson, Tarja Pitkanen, Veronica Rajal, Brandon Reyneke, Fernando Roman, Joan Rose, Marta Rusinol, Michael Sadowsky, Laura Sala-Comorera, Yin Xiang Setoh, Samendra Sherchan, Kwanrawee Sirikanchana, Wendy Smith, Joshua Steele, Rosalie Sabburg, Erin Symonds, Phong Thai, Kevin Thomas, Josh Tynan, Simon Toze, Janelle Thompson, Andy Whiteley, Judith Wong, Daisuke Sano, Stefan Wuertz, Irene Xagoraraki, Qian Zhang, Amity Zimmer-Faust, Orin Shanks

Subject: Environmental And Earth Sciences, Waste Management And Disposal Keywords: COVID-19; SARS-CoV-2; Wastewater; Surveillance; False-positive; False-negative; RT-PCR

Online: 19 April 2021 (13:08:13 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Wastewater surveillance for pathogens using the reverse transcription-polymerase chain reaction (RT-PCR) is an effective, resource-efficient tool for gathering additional community-level public health information, including the incidence and/or prevalence and trends of coronavirus disease-19 (COVID-19). Surveillance of SARS-CoV-2 in wastewater may provide an early-warning signal of COVID-19 infections in a community. The capacity of the world’s environmental microbiology and virology laboratories for SARS-CoV-2 RNA characterization in wastewater is rapidly increasing. However, there are no standardized protocols nor harmonized quality assurance and quality control (QA/QC) procedures for SARS-CoV-2 wastewater surveillance. This paper is a technical review of factors that can lead to false-positive and -negative errors in the surveillance of SARS-CoV-2, culminating in recommendations and strategies that can be implemented to identify and mitigate these errors. Recommendations include, stringent QA/QC measures, representative sampling approaches, effective virus concentration and efficient RNA extraction, amplification inhibition assessment, inclusion of sample processing controls, and considerations for RT-PCR assay selection and data interpretation. Clear data interpretation guidelines (e.g., determination of positive and negative samples) are critical, particularly during a low incidence of SARS-CoV-2 in wastewater. Corrective and confirmatory actions must be in place for inconclusive and/or potentially significant results (e.g., initial onset or reemergence of COVID-19 in a community). It will also be prudent to perform inter-laboratory comparisons to ensure results are reliable and interpretable for ongoing and retrospective analyses. The strategies that are recommended in this review aim to improve SARS-CoV-2 characterization for wastewater surveillance applications. A silver lining of the COVID-19 pandemic is that the efficacy of wastewater surveillance was demonstrated during this global crisis. In the future, wastewater will play an important role in the surveillance of a range of other communicable diseases.

Preprint REVIEW | doi:10.20944/preprints202004.0201.v2

Detecting the Coronavirus (COVID-19)

Pravin Pokhrel, Changpeng Hu, Hanbin Mao

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: SARS-CoV-2 Detection, SARS-CoV-2 Antibody Test, SARS-CoV-2 Antigen Test, False Negative, False Positive, Sensitivity, Specificity, Point-of-care testing (POCT), SARS-CoV-2 Mutants

Online: 25 March 2021 (15:33:14 CET)

Show abstract| Download PDF| Share

The COVID-19 pandemic has created huge damage to society and brought panics around the world. Such panics can be ascribed to the seemingly deceptive features of the COVID-19: compared to other deadly viral outspreads, it has medium transmission and mortality rates. As a result, the severity of the causative coronavirus, SARS-CoV-2, was deeply underestimated by the society at the beginning of the COVID-19 outbreak. Based on this, in this review, we define the viruses with features similar to those of SARS-CoV-2 as the Panic Zone viruses. To contain those viruses, accurate and fast diagnosis followed by effective isolation and treatment of patients are pivotal at the early stage of virus breakouts. This is especially true when there is no cure or vaccine available for a transmissible disease, which is the case for current COVID-19 pandemic. As of January 2021, more than two hundred kits for the COVID-19 diagnosis on the market are surveyed in this review, while emerging sensing techniques for SARS-CoV-2 are also discussed. It is of critical importance to rationally use these kits for the efficient management and control of the Panic Zone viruses. Therefore, we discuss guidelines to select diagnostic kits at different outbreak stages of the Panic Zone viruses, SARS-CoV-2 in particular. While it is of utmost importance to use nucleic acid-based detection kits with low false negativity (high sensitivity) at the early stage of an outbreak, the low false positivity (high specificity) gains its importance at later stages of the outbreak. When a society is set to reopen from the lock-down stage of the COVID-19 pandemic, it becomes critical to have antibody based immunoassay kits with high specificity to identify people who can safely return to the society after their recovery of SARS-CoV-2 infections. Given that the emergence of mutant viruses at the beginning of 2021 has complicated current battle against the COVID-19, we also discussed approaches and guidelines to detect viral mutants in the middle of the second wave of the pandemic that started at the end of 2020. Finally, since a massive attack from a viral pandemic requires a massive defense from the whole society, we urge both government and private sectors to research and develop more affordable and reliable point-of-care testing (POCT) kits, which can be used massively by the general public (and therefore called as massive POCT) to contain Panic Zone viruses in future.

Preprint ARTICLE | doi:10.20944/preprints202012.0094.v3

“Clinician’s Probability Calculator” to Convert Pre-Test to Post-Test Probability of COVID-19, Based on Method Validation from Each Laboratory

Zoe Brooks, Saswati Das, Tom Pliura

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: COVID-19; SARS-COV-2; False-Positive; False-Negative; Likelihood Ratio; Probability; Calculator; Interpretation

Online: 3 March 2021 (10:08:27 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints201608.0049.v2

Understanding Farmers’ Decision Making in Agricultural Water Fee Payment in China: The Role of Mental Accounting

Weikang Zhang, Xinhong Fu, Jing Lu, Lin Zhang, Kwamega Michael, Guoqiang Liu, Yuying Liu, Fan Yang

Subject: Business, Economics And Management, Economics Keywords: mental accounting; agricultural water fee; behavioral economics; decision making; information processing; representativeness; negative psychological externalities

Online: 25 August 2016 (10:12:08 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201807.0224.v2

Pseudopterosin Inhibits Proliferation and 3D Invasion in Triple Negative Breast Cancer by Agonizing Glucocorticoid Receptor Alpha

Julia Sperlich, Nicole Teusch

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: pseudopterosin; triple negative breast cancer; glucocorticoid receptor alpha; dexamethasone; cell proliferation; 3D invasion; tumor spheroid; co-culture; interleukin 6; interleukin 8

Online: 2 August 2018 (06:08:31 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202212.0450.v1

Klein-Gordon, Dirac and Schrodinger Equations, Matter and Wave Function Collapses, Photon Momenta and the Gamma Ray Burst based on the 4-D Euclidean Space

Jae-Kwang Hwang

Subject: Physical Sciences, Particle And Field Physics Keywords: P4 = TcP symmetry; Matter collapse; Wave function collapse; Klein paradox; Continuity equations; Negative energy solutions; Gamma ray burst; Photon momenta

Online: 23 December 2022 (07:57:12 CET)

Show abstract| Download PDF| Share

The negative energy solutions of the Klein-Gordon and Dirac equations have been used to define the antiparticle with the positive energy from the Feynman - Stueckelberg interpretation in the standard model based on the 4-D Minkowski space with the relative time. The negative energy solutions of the Klein-Gordon and Dirac equations are used to define the antiparticles with the negative energy in the present 3-D quantized space model. Note that all the present results are based on the 4-D Euclidean space with the absolute time (t) and relative time (t_l). Then the particle with the positive energy and antiparticle with the negative energy have the 4-D P₄ = TCP = T_cP symmetry. The continuity equations are derived from the Klein-Gordon, Dirac and Schrodinger equations. The Klein-Gordon and Schrodinger equations with the potential barriers are solved. The Klein paradox is explained by using the pair production of the electron with the positive energy and positron with the negative energy under the P₄ = _TcP symmetry, The effective velocity ( c_eff=√2 " c " ) and effective energy ("Etl =" √2 E ) along the relative time axis (ctl) give the relative time momentum of "p_tl"=E_tl/c_eff =(√2 E)/(√2 c)=E/c=p_t=p_x. This indicates that the photons with the energy of E have the constant photon velocity of c and the constant photon momentum of "ptl"=E_tl/c_eff =(√2 E)/(√2 c)=E/c=p_t=p_x. For the negative energy particles, the space momentum directions are opposite to the particle velocity directions because the energy is negative. And the matter collapse to the photons at the very high particle velocity is discussed from the length expansion. It is, for the first time, proposed that the gamma ray burst is originated from the matter collapse to the photons near the black hole. Also, the wave function collapse is explained from the length expansion. The wave function collapse takes place when the measurement makes the particle velocity to be zero. It is concluded that the negative energy solutions of the Klein-Gordon and Dirac equations support the existence of the partner antimatter universe with the negative energy and negative time direction. Our matter universe has the positive energy and the positive time direction.

Preprint ARTICLE | doi:10.20944/preprints201608.0144.v1

Statistical Study on Crash Frequency Model Using GNB Models of Freeway Sharp Horizontal Curve Based on Interactive Influence of 3 Explanatory Variables

Yanjie Zeng, Xiaofei Wang, Lineng Liu, Xinwei Li, Caifeng Jiang

Subject: Engineering, Civil Engineering Keywords: freeway; crash prediction model (CPM); sharp horizontal curve segment (SHCS); interactive influence among three explanatory variables; generalized negative binomial (GNB)

Online: 15 August 2016 (09:47:37 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201706.0077.v1

Are Mobile Live Animal Programs Educationally Beneficial? A Critical Assessment of the Human Learning Component Underlying Traveling Animal Exhibits

Kathryn Sussman

Subject: Social Sciences, Education Keywords: zoo; mobile zoo; mobile animal exhibits; animal display; mobile live animal programs; negative education

Online: 16 June 2017 (05:32:18 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201609.0098.v1

Oyelola A. Adegboye, Majeed Adegboye

Subject: Computer Science And Mathematics, Probability And Statistics Keywords: ecological niche model; environment; overdispersion; negative binomial; leishmaniasis; infectious disease

Online: 27 September 2016 (10:17:31 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202107.0614.v1

Mixed Effects and Mechanisms of Cannabinoids for Triple-Negative Breast Cancer Treatment

Khanh Tran

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: Triple-negative breast cancer; cannabinoid; marijuana; cell signalling; medicinal plants

Online: 28 July 2021 (08:56:32 CEST)

Show abstract| Download PDF| Share

Triple-negative breast cancer (TNBC) is a subtype of breast cancer characterized by the lack of estrogen receptors (ER), progesterone receptors, and HER-2 receptors. Thus, TNBC tumours do not benefit from the current therapies targeting ER or HER-2. Therefore, there is an urgent need to develop novel treatment for this subtype of breast cancer. Marijuana is a common name given to Cannabis plants, a group of plants in the Cannabis genus of the Cannabaceae family. Cannabis plants are among the oldest cultivated crops, traced back at least 12,000 years and are well known for their multi-purpose usage, including medicinal purposes. The main active compounds extracted from Cannabis plants are 21-carbon-containing terpenophenolics, which are referred to as phytocannabinoids. Of these, the tetrahydrocannabinol (THC) group contains highly potent cannabinoids, including delta-9-tetrahydrocannabinol (∆9-THC) and delta-8-tetrahydrocannabinol (∆8-THC), which are the most abundant THCs and are largely responsible for psychological and physiological effects of marijuana. The use of Cannabis plants for medicinal purposes was first recorded in 2337 BC in China, where Cannabis plants were used to treat pains, rheumatism, and gout. Recently, several cannabinoids have been approved for a number of treatments, one of which is the treatment of nausea and vomiting caused by chemotherapy in cancer patients. Furthermore, increasing evidence shows that cannabinoids not only attenuate side effects due to cancer treatment, but might also potentially possess direct antitumor effects in several cancer types, including breast cancer. However, anti-tumour activity of marijuana has been variable in different studies and even promoted tumour growth in some cases. In addition, the mechanisms of cannabinoid action in cancer remain unclear. This review summarizes evidence about the mixed actions of cannabinoids in cancer in general and triple-negative breast cancer in particular.

Preprint ARTICLE | doi:10.20944/preprints201612.0104.v1

Internalization of Negative External Cost of Logistics and Incentive Mechanism

Zhanzhong Wang, Zhouling Tsai, Jing Fu, Guangdong Tian

Subject: Engineering, Control And Systems Engineering Keywords: green logistics; negative externality of logistics; internalization of logistics negative external cost; incentive mechanism

Online: 20 December 2016 (10:37:21 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202006.0279.v2

COVID-19 Testing – Impact of Prevalence, Sensitivity, and Specificity on Patient Risk and Cost

Zoe C. Brooks, Saswati Das

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: COVID19; risk; clinical; metrics; cost; false-positive; false-negative; prevalence; sensitivity; specificity

Online: 9 July 2020 (15:57:30 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202004.0117.v1

Using Negative Pressure Wound Therapy in the Treatment of Wounds After Fasciotomy in Acute Compartment Syndrome - Retrospective Case Series Study

Jaroslaw P. Miszczuk, Maria Błońska-Staniec, Anna Michalska, Bartosz Stemplewski

Subject: Medicine And Pharmacology, Surgery Keywords: acute compartment syndrome; negative pressure wound therapy; vacuum assisted wound closure; fasciotomy

Online: 8 April 2020 (04:50:32 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202011.0626.v1

Isolation and Identification of Gram Positive and Negative Bacteria from Dairy Department Waste Lines at Baraton University

Stella Wanyama

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Isolation; Identification; gram positive; gram negative; tests staining; microbio metabolic activities

Online: 25 November 2020 (08:36:32 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201705.0137.v1

Investigation on Development of Sliding Mode Controller for Constant Power Loads in Microgrids

Eklas Hossain, Ron Perez, Sanjeevikumar Padmanaban, Pierluigi Siano

Subject: Engineering, Electrical And Electronic Engineering Keywords: sliding mode control; constant power load; negative incremental impedance; robustness analysis; chattering reduction; microgrid stability; noise rejection

Online: 18 May 2017 (04:45:56 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202109.0083.v1

Is the Higgs Field a Positive and Negative Mass Planckion Condensate, and Does the LHC Produce Extreme Dark Energy?

Christopher Pilot

Subject: Physical Sciences, Particle And Field Physics Keywords: Winterberg Model; Quantum Vacuum; Higgs Condensate/Composite Model; Two-Component Superfluid/Supersolid Model for the Vacuum; Positive/Negative Planckion Mass, Q-Theory; Non- Linear Heisenberg Spinor Theory; LHC Energies, Dark Energy; Lattice Substructure for Space

Online: 6 September 2021 (10:38:45 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202202.0026.v1

Dark Energy is Dark Matter below Absolute Zero in Black Holes

Roumen Tsekov

Subject: Physical Sciences, Astronomy And Astrophysics Keywords: Neutrino, Dark Matter, Dark Energy, Black Holes, Negative Temperature

Online: 2 February 2022 (09:35:52 CET)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints201706.0059.v1

Pathology of Adult and Paediatric Mitochondrial Myopathies

Rahul Phadke

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: mitochondrial; muscle biopsy; ragged red; COX-negative; subsarcolemmal; immunohistochemistry

Online: 13 June 2017 (06:23:36 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202107.0626.v1

A High Efficacy DNA Vaccine Against Tilapia Lake Virus in Nile Tilapia (Oreochromis niloticus)

Nai-tong Yu, Wei-wei Zeng, Jian-hua Wang, Yu-liang Zhang, Xiu-chun Zhang, Zhi-xin Liu

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: tilapia; negative-sense RNA virus; Tilapia lake virus; DNA vaccine

Online: 28 July 2021 (11:11:37 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202105.0546.v1

Atezolizumab and Pembrolizumab in Triple-Negative Breast Cancer: A Meta-Analysis

Mahwish Amin, Shaiza Sharif, Waleed Asghar, Humaira Sadaf, Azza Sarfraz, Zouina Sarfraz, Ivan Cherrez-Ojeda

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: triple negative breast cancer; Pembrolizumab; Atezolizumab; chemotherapy; anti-PD-L1; biomarkers; targeted therapies; development of novel drugs

Online: 24 May 2021 (09:23:50 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201805.0026.v1

Weighted Negative Binomial Poisson-Lindley Distribution with Actuarial Applications

Hossein Zamani, Noriszura Ismail, Marzieh Shekari

Subject: Computer Science And Mathematics, Probability And Statistics Keywords: weighted distribution; Poisson-Lindley distribution; discrete distribution; weighted negative binomial Poisson-Lindley distribution

Online: 2 May 2018 (11:47:51 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202101.0147.v1

A Proposed Model of Dehumanisation and Its Relevance to Dehumanisation in Dementia Care

Hana Speering, Lara Speering

Subject: Social Sciences, Psychology Keywords: Dehumanisation; dementia care; healthcare education; residential aged care facilities; positive dehumanisation; negative dehumanisation

Online: 8 January 2021 (11:23:42 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201709.0127.v2

A New Proof of Smoryński’s Theorem

Apoloniusz Tyszka

Subject: Computer Science And Mathematics, Logic Keywords: Davis-Putnan Robinson-Matiyasevich theorem; Diophantine equation which has at most finitely many solutions in non-negative integers; Diophantine equation which has at most finitely many solutions in positive integers; Diophantine equation which has at most finitely many integer solutions; Hilbert’s Tenth Problem; Matiyasevich’s theorem; recursively enumerable set; Smoryński’s theorem

Online: 27 September 2017 (04:05:45 CEST)

Show abstract| Download PDF| Share

Working Paper ARTICLE

Turkish Adaptation of the Positive-Negative Relationship Quality (PN-RQ) Scale: A Reliability and Validity Study

Arzu Araz, Duygu Güngör, Eda Aşçı

Subject: Social Sciences, Psychology Keywords: positive-negative relationship quality; reliability; validity; confirmatory factor analysis (CFA); bifactor

Online: 16 September 2019 (16:33:17 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202101.0076.v6

Unification of the Electromagnetic Force and Gravity through the Composite Photon

Ian Clague

Subject: Physical Sciences, Acoustics Keywords: Unification; Composite Photon; Negative Mass; Antimatter; Grand Unification Theory, Dark Energy, Dark Matter

Online: 28 October 2021 (10:06:39 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202103.0777.v1

Prediction of Response to Atezolizumab Plus Nab-Paclitaxel in Unresectable Locally Advanced Triple Negative Breast Cancer (TNBC): the ClinicalUsefulness of PD-L1 mRNA Expression in Plasma-Derived Exosomes

Lucrezia Raimondi, Gian Paolo Spinelli, Paolo Ciracì, Filippo Maria Raimondi, Rachele Lazzeroni, Laura Di Benedetto, Stefano Valabrega, Laura Giaconi, Luigi Rossi, Giuseppe Naso

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Exosomal PD-L1 mRNA; extracellular vesicles; Triple Negative Breast Cancer; Immunotherapy; PD-L1 axis; Atezolizumab – nab-paclitaxel; Predictive biomarkers; Liquid biopsy

Online: 31 March 2021 (15:27:45 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints201802.0095.v1

EP4 as a Therapeutic Target for Aggressive Human Breast Cancer

Mousumi Majumder, Pinki Nandi, Ahmed Omar, Kingsley Chukwunonso Ugwuagbo, Peeyush K. Lala

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: COX-2; breast cancer; PGE2; EP receptors; stem-like cells; metastasis; angiogenesis; lymphangiogenesis; MicroRNAs; triple negative breast cancer

Online: 13 February 2018 (09:16:54 CET)

Show abstract| Download PDF| Share

G protein-coupled receptors (GPCRs, also called seven-transmembrane or heptahelical receptors) are a superfamily of cell surface receptor proteins that bind to many extracellular ligands and transmit signals to an intracellular guanine nucleotide-binding protein (G protein). When a ligand binds, the receptor activates the attached G-protein by causing the exchange of Guanosine-5'-triphosphate (GTP) for guanosine diphosphate (GDP). They play a major role in many physiological functions as well as in the pathology of many diseases including cancer progression and metastasis. Only a few GPCR members have been exploited as targets for developing drugs with therapeutic benefit in cancer. Present review deals with the Prostaglandin E receptor EP4, a member of the EP family of GPCR, as a promising newer therapeutic target for treating breast cancer. We show that aberrant over-expression of cyclooxygenase (COX)-2, an inflammation-associated enzyme, occurring in 40-50% of breast cancer patients leads to tumor progression and metastasis due to multiple cellular events resulting from an increased prostaglandin (PG) E2 production in the tumor milieu. They include inactivation of host anti-tumor immune (NK and T) cells, increased immuno-suppressor function of tumor-associated macrophages, promotion of tumor cell migration, invasiveness and tumor-associated angiogenesis (due to upregulation of VEGF-A), lymphangiogenesis (due to upregulation of VEGF-C/D) and a stimulation of stem-like cell (SLC) phenotype in cancer cells. All these events were primarily mediated by activation of the PGE receptor EP4 on tumor or host cells. We show that selective EP4 antagonists (EP4A) could mitigate all these events tested with cells in vitro as well as in vivo in syngeneic COX-2 expressing mammary cancer bearing mice or immune-deficient mice bearing COX-2 over-expressing human breast cancer xenografts. We suggest that EP4A can avoid thrombo-embolic side effects of long term use of COX-2 inhibitors by sparing cardio-protective roles of PGI2 via IP receptor activation or PGE2 via EP3 receptor activation. Furthermore, we identified two COX-2/EP4 induced oncogenic and SLC-stimulating microRNAs - miR526b and miR655, one of which (miR655) appears to be a potential blood biomarker in breast cancer patients, for monitoring SLC-ablative therapies such as with EP4A. We suggest that EP4A will likely produce the highest benefit in aggressive breast cancers such as COX-2 expressing triple-negative breast cancers, when combined with other newer agents such as PD-1 or PD-L1 inhibitors.

Preprint ARTICLE | doi:10.20944/preprints201803.0174.v1

Whole Genome Transcriptome Analysis of the Association between Obesity and Triple Negative Breast Cancer in Caucasian Women

Tarun Mamidi, Jiande Wu, Paul B. Tchounwou, Lucio Miele, Chindo Hicks

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: gene expression obesity triple negative breast cancer

Online: 20 March 2018 (07:56:33 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints201801.0100.v1

Pressure Distribution during Negative Pressure Wound Therapy of Experimental Abdominal Compartment Syndrome in a Porcine Model

Adrienn Csiszkó, Klaudia Balog, Zoltán Attila Godó, Gyula Juhász, Katalin Pető, Ádám Deák, Mariann Berhés, Norbert Németh, Zsolt Bodnár, Zsolt Szentkereszty

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: intra-abdominal pressure; abdominal compartment syndrome; pressure sensor; negative pressure wound therapy; open abdomen;

Online: 11 January 2018 (09:17:10 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202011.0056.v1

Exploring the Non-Medical impacts of Covid-19 using Natural Language Processing

Amol Agade, Samta Balpande

Subject: Computer Science And Mathematics, Algebra And Number Theory Keywords: COVID-19; Deep Learning; Natural Language Processing; Topic Modelling; Text Classification; Latent Dirichlet allocation (LDA); Non-negative matrix factorization (NMF)

Online: 2 November 2020 (15:24:20 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202109.0085.v1

Chick Embryo Experimental Platform for 3D Tissue Engineering Modelling of Cancer Micrometastases for Tumor Biology, Drug Development and Nanomaterials Testing

Anna Guller, Inga Kuschnerus, Vlada Rozova, Annemarie Nadort, Yin Yao, Zahra Khabir, Alfonso Garcia-Bennett, Liuen (Olivia) Liang, Aleksandra Polikarpova, Yi Qian, Ewa M. Goldys, Andrei Zvyagin

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: chick embryo; 3D culture; tumor models in vitro; tissue engineering; metastasis; triple-negative breast cancer; liver; mesoporous silica nanoparticles; doxorubicin; micrometastasis

Online: 6 September 2021 (11:46:54 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202004.0178.v1

Identification of Distinct Heterogenic Subtypes and Molecular Signatures Associated With African Ancestry in Triple Negative Breast Cancer Using Quantified Genetic Ancestry Models in Admixed Race Populations

Melissa Davis, Rachel Martini, Lisa Newman, Olivier Elemento, Jason White, Akanksha Verma, Indrani Datta, Indra Adrianto, Kevin Gardner, Hyung-Gyoon Kim, Windy Dean-Colomb, Isam Eldin Eltoum, Andra R Frost, William E. Grizzle, Andrea Sboner, Upender Manne, Clayton Yates

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: triple negative breast cancer; African Ancestry; RNAseq analysis; oncologic pathways

Online: 12 April 2020 (04:22:51 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints201908.0059.v1

The Effect of Recreation in a Snow-Covered Forest Environment on the Psychological Relaxation of Young Females

Ernest Bielinis, Adrian Łukowski, Aneta Omelan, Sergii Boiko, Norimasa Takayama

Subject: Biology And Life Sciences, Forestry Keywords: deciduous forest; female; forest bathing; forest therapy; Positive and Negative Affect Schedule; Profile of Mood States; Restorative Outcome Scale; restoration; Shinrin-Yoku; snow covered forest; Subjective Vitality Scale; winter

Online: 5 August 2019 (08:56:32 CEST)

Show abstract| Download PDF| Share

Forest recreation can be successfully conducted for the purpose of psychological relaxation, as has been proven in previous scientific studies. During the winter in many countries, when snow cover occurs frequently, forest recreation (walking, relaxation, photography, etc.) is common. Nevertheless, whether forest therapy conducted in a forest environment with a snow cover will also have a positive effect on psychological indicators remains unknown. Furthermore, male subjects frequently participate in forest therapy experiments, whereas females are rarely involved. Thus, in this study, the effectuality of forest recreation during winter and with snow cover was tested on 32 young females. For these reasons, the experiment involved 15-minute periods of relaxation in a forest environment or in an urban environment, in addition to a pre-test under indoor conditions. Four psychological questionnaires (POMS, PANAS, ROS, SVS) were administered to participants before and after interventions. Results showed that participants’ levels of negative mood, as measured by different aspects of the POMS questionnaire (tension-anxiety, anger-hostility, depression-dejection, confusion, fatigue), decreased after exposure to the forest environment. In contrast, both tension-anxiety and anger-hostility increased in the urban environment. The indicator of negative affect from the PANAS questionnaire also increased after exposure to the urban environment, whereas the indicator of positive affect based on PANAS was higher in the forest environment than in the urban environment. Restorativeness and subjective vitality exhibited higher values after exposure to the forest environment in comparison to those from the control and pre-test. The changes in these indicators demonstrates that forest recreation in the snow during winter can significantly increase psychological relaxation in young females, as well as showing that recreation can be successfully conducted under these winter conditions.

Preprint ARTICLE | doi:10.20944/preprints201802.0163.v1

Shaping Light in Backward-Wave Nonlinear Hyperbolic Metamaterials

Alexander K. Popov, Sergey A. Myslivets, Vitaly V. Slabko, Victor A. Tkachenko, Thomas F. George

Subject: Physical Sciences, Optics And Photonics Keywords: optical metamaterials; fundamental concepts in photonics; light-matter interactions at the subwavelength and nanoscale; fundamental understanding of linear and nonlinear optical processes in novel metamaterials underpinning photonic devices and components; advancing the frontier of nanophotonics with the associated nanoscience and nanotechnology; nanostructures that can serve as building blocks for nano-optical systems; use of nanotechnology in photonics; nonlinear nanophotonics, plasmonics and excitonics; subwavelength components and negative index materials; slowing, store, and processing light pulses; materials with such capabilities that could be used for optical sensing, tunable optical delay lines, optical buffers, high extinction optical switches, novel image processing hardware, and highly-efficient wavelength converters

Online: 26 February 2018 (11:24:39 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202012.0703.v5

On the Fundamental Particles and Reactions of Nature

Jian-Bin Bao, Nicholas P. Bao

Subject: Physical Sciences, Quantum Science And Technology Keywords: aether; hypoatom; neutrino; inflation; gravity; negative pressure; dark energy; dark matter; black hole; spinning universe; quantum mechanics; first galaxies; spin-1/2 particle; matter-antimatter asymmetry; baryogenesis-through-leptogenesis; fabric of space; conformal cyclic cosmology; center of the universe; fate of the universe; general theory of relativity; parity non-conservation in weak interactions

Online: 4 January 2023 (02:26:55 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201911.0033.v4

Circuit-Based Approaches for a Superconducting-Like Behavior with Relatively High Critical Current Density

Shinichi Ishiguri

Subject: Physical Sciences, Condensed Matter Physics Keywords: temperature-independent superconductivity; circuit-approached superconductivity; electron pair; Bose– Einstein condensation; large superconducting energy gap; London equation; Meissner effect; macroscopic wave function; critical current density; negative voltages

Online: 20 February 2024 (22:14:28 CET)

Show abstract| Download PDF| Share

In general, a superconductor has zero resistance, although it requires significant refrigeration or high pressures with high manufacturing costs, which prevents it into practical applications. In other words, solving these issues implies main superconducting researches. To solve these problems, this paper describes a new type of superconductivity, which is independent for temperatures and which operates without pressures. The principles of the presented system are as follows:First a voltage source, a current source and a load are connected in series. Then, the voltage of the voltage source is adjusted to balance the voltage of the load. Under this condition, the balance of the two voltages provides a zero voltage between the taps of the current source and the generated current from the voltage source becomes zero because of the internal infinite resistance of the current source. As a result, the electric powers generated by the two sources are zero, and therefore, the load cannot generate Joule heating because of energy conservation. However, the current from the current source (not the voltage source) is not zero; therefore, we can predict that the resistance of the load must be zero. As a theory, we derived a new electric field and transient attractive force, which result in a very short coherence of an electron pair because there is not the Coulomb repulsive force due to the existence of the above transient attractive force. Note that both the forces are derived by the Poisson equation, which implies that they cannot be compatible. Therefore, the pair combination energy from spins becomes extremely strong, which is not destroyed by the normal heat energy. Moreover, every center-of-mass motion of electron pair results in the Bose-Einstein condensation and the macroscopic wave function, which produces the London equation (i.e., the Meissner effect). Moreover, by introducing the equivalent circuit, this paper conducted numerical calculations. As a result, we could derive numerically zero resistance and responses for additional static magnetic fields as a discharged current, which implies the Meissner effect. Note that this paper has prepared Appendix section, which provides a guide to reproduce actual experiments and preliminary experimental results. These results in the Appendix indicate the zero resistances and Meissner effects.

Preprint ARTICLE | doi:10.20944/preprints201911.0368.v1

The Role of Perceived Social Support in the Psychological Well-Being of Young Offenders: from Pretrial Detention to Correctional Camps

Fedor Ushkov, Roman Shilko, Ludmila Shaigerova, Alexandra Dolgikh, Olga Almazova, Olga Vakhantseva, Yuri Zinchenko

Subject: Social Sciences, Psychology Keywords: psychological well-being; young offenders; social support; pretrial detention; correctional camps; positive and negative feelings; positive and negative affects

Online: 29 November 2019 (04:16:05 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202104.0433.v1

SRT as a Fourth-order-theory with Analogy-model of Damped Resonance

Holger Döring

Subject: Physical Sciences, Acoustics Keywords: SRT; fourth-order-theory; any velocity; FTL; real rest mass; resonance analogy; damped space-time; SR-Extrapolation; superluminal-velocity; negative kinetic energy; fourth-order worldline-element; no-tachyons

Online: 16 April 2021 (10:42:35 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201908.0225.v1

Analysis of Water Bodies under Partial Cloud Conditions Using Satellite Images

Rahul Neware, Mansi Thakare

Subject: Environmental And Earth Sciences, Remote Sensing Keywords: water bodies; satellite images; vector data; SVM; positive and negative buffering; polygons

Online: 21 August 2019 (10:30:16 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202208.0511.v1

Phytochemical Composition and Antibacterial Activity of Zingiber cassumunar Roxb. against Agricultural and Foodborne Pathogens

Maha A. Alshiekheid, Yheni Dwiningsih, Amal Abdullah Sabour, Jawaher Alkahtani

Subject: Biology And Life Sciences, Plant Sciences Keywords: Zingiber cassumunar Roxb.; essential oils; microencapsulated products; antibacterial activity; agricultural pathogens; foodborne pathogens; gram-positive bacteria; gram-negative bacteria

Online: 30 August 2022 (07:02:54 CEST)

Show abstract| Download PDF| Share

Zingiber cassumunar Roxb. is a powerful medicinal plant that has been used as traditional medicine to cure respiratory problems, pain, and inflammation in China, Indonesia, Thailand and other Asian countries by using the crude extracts. The objective of this research is to identify phytochemical composition of Z. cassumunar Roxb. and to analyze antibacterial activity of crude extract, purified compounds, and their microencapsulated products of Rhizome Z. cassumunar Roxb. Identification of phytochemical composition in crude extract of rhizome Z. cassumunar Roxb. was achieved by chromatography-mass spectrophotometer. The major phytochemical composition in crude extract of Z. cassumunar Roxb. is essential oils, including terpinen4-ol (37.7%), β-pinene (20.8%), and (E)-1-(3,4-dimethoxyphenyl)but-1-ene (13.3%). Crude extract of Z. cassumunar Roxb. was purified with silica gel flash column chromatography, resulting two purified compounds. The antibacterial activity of crude extract, purified compounds, and their microencapsulated products of Rhizome Z. cassumunar Roxb. were evaluated against agricultural and foodborne pathogens by using disc agar diffusion and broth microdilution techniques. All of the samples studied (crude extracts, purified compounds, and microencapsulated of Z. cassumunar Roxb.) were effective against all the bacteria. Based on the results of the disc-diffusion assay suggested that amongst the samples studied, purified compounds (compound 1 and 2) and microencapsulated purified compounds (compound 1 and 2) exhibited more effective against all the bacteria compared to the crude extracts. Antibacterial activity of the rhizome of Z. cassumunar Roxb. was contributed mainly by the essential oils components as the active compounds. Gram-negative bacteria (X. oryzae, X. translucens, Pseudomonas spp, E. coli, and S. typhimurium) appeared to the most resistant to the crude extracts, purified compounds, and microencapsulated of Z. cassumunar Roxb. compared to the gram-positive bacteria (S. aureus, B. cereus, and L. monocytogenes). Microencapsulated of the tested samples (crude extract, purified compound 1, and purified compound 2) of the rhizome Z. cassumunar Roxb. exhibited high antibacterial activity with no significantly different with the tested samples without microencapsulation. These results suggest potential antibacterial properties of Z. cassumunar Roxb., which useful for agricultural plant health, food preservation, natural therapies, and pharmaceuticals.

Preprint ARTICLE | doi:10.20944/preprints201910.0217.v1

Aptamer Bead-Mediated Separation of Gram-Negative Bacteria Using Acoustophoresis

Sang Woo Lee, Byungw Woo Kim, Hye-Su Shin, Anna Go, Minho Lee, Dong-ki Lee, Soyoun Kim, Ok Chan Jeong

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: aptamer: acoustophoresis; microfluidics; gram-negative bacteria

Online: 18 October 2019 (11:41:41 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202107.0238.v1

An Idea about Negative Cosmic Time in the Big Bang-Big Rip Cosmological Model

Mohamed Abdalla Bakry, Ali Eid, A. Alkaoud

Subject: Physical Sciences, Acoustics Keywords: Negative cosmic time; cosmological solutions; variable deceleration parameter; big rip

Online: 12 July 2021 (09:46:04 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202004.0509.v1

GATA 3 Gene Overexpression Is A Biomarker of Adverse Prognosis for Peripheral T-Cell Lymphoma, Not Otherwise Specified and for ALK-Negative Anaplastic Large Cell Lymphoma: An Exploratory Analysis of 80 Latin American Cases of nodal PTCLs

Luís Alberto de Pádua Covas Lage, Cláudio Vinícius Brito, Débora Levy, Hebert Fabrício Culler, Samuel Campanelli Freitas Couto, Lucas Bassolli, Maria Claudia Nogueira Zerbini, Vanderson Rocha, Juliana Pereira

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: nodal peripheral T-cell lymphomas; peripheral T-cell lymphoma, not otherwise specified (PTCL/NOS); ALK-negative anaplastic large-cell lymphoma (ALCL/ALK-); Angioimmunoblastic T-cell lymphoma (AITL); diagnosis; prognosis; GATA3 gene expression

Online: 29 April 2020 (12:48:00 CEST)

Show abstract| Download PDF| Share

Background: Nodal peripheral T-cell lymphomas (nPTCLs) encompass a heterogeneous group of mature and aggressive lymphoid malignancies, including peripheral T-cell lymphoma, not otherwise specified (PTCL/NOS), angioimmunoblastic T-cell lymphoma (AITL) and anaplastic large cell lymphoma (ALCL) ALK-positive and ALK-negative. Their differential diagnosis and prognosis are an issue in the clinical practice. Accurate biomarkers to refine the different subtypes of nPTCLs and to stratify their prognosis are essential to improve their treatment approach. The aim of this study was to test the prognostic impact of GATA3 gene expression, and its capability to discriminate the different subtypes of nPTCLs. Patients and Methods: From 2000 to 2017, 80 patients with nPTCLs were eligible for GATA3 gene expression analysis that was assessed retrospectively by quantitative real time PCR (qRT-PCR) of neoplastic biopsies in Formalin-Fixed Paraffin-Embedded samples (FFPE). Results: Median age was 49 years old (IqR 34-59), 43/80 (53.7%) were male. Median follow-up was 1.72 years. Of them, 36.3% were classified as PTCL/NOS, 31.2% ALK-negative ALCL, 21.2% ALK-positive ALCL and 11.3% AITL. The majority of cases had advanced stage (III/IV). Two-year estimated overall survival (OS) and progression-free survival (PFS) were 52.2% and 39.5%, respectively. The median GATA3 gene expression level was 0.49% (range 0 – 7.07) in all cohort, it was 0.11% for ALK-positive ALCL, 0.46% for ALK-negative ALCL, 0.86% for PTCL/NOS and 0.67% for AITL. The difference of GATA3 gene expression among distinct variants of nPTCLs was statistically significant (p < 0.001). GATA3 gene expression levels ≥ 0.71% discriminate PTCL/NOS from ALK-negative ALCL and AITL with sensitivity of 62% and specificity of 80.3%. GATA3 gene expression levels ≥ median was associated with poor 2-year OS for PTCL/NOS (46.7% x 21.4%, p=0.04) and for ALK-negative ALCL (85.7% x 54.5%, p=0.04). Conclusion: Despite the relative small and heterogeneous group of patients with nPTCLs, GATA3 gene overexpression may be an important biomarker associated with poor prognosis in PTCL/NOS and ALK-negative ALCL. Moreover it may also discriminate different subtypes of nPTCLs. Further studies with larger series of patients should confirm our findings.

Preprint ARTICLE | doi:10.20944/preprints202104.0688.v1

Direct Quantitation of SARS-CoV-2 Using Droplet Digital PCR in Suspected Samples With Very Low Viral Load

Michela Deiana, Chiara Piubelli, Antonio Mori, Gian Paolo Chiecchi, Giulia La Marca, Martina Leonardi, Davide Treggiari, Anna Beltrame, Lucia Moro, Zeno Bisoffi, Elena Pomari

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: droplet digital PCR; real time RT-PCR; SARS-CoV-2; false negative; viral load; diagnosis

Online: 26 April 2021 (20:09:26 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202306.1716.v1

A Physical Mechanism of the Generation of Stable Positive Kinetic Energy Systems and a Qualitative Explanation of the Proportions of the Four Ingredients in the Universe

Huai-Yu Wang

Subject: Physical Sciences, Quantum Science And Technology Keywords: stable system; dark matter; dark energy; positive kinetic energy; negative kinetic energy; cosmic dark radiation background; gravity potential background

Online: 25 June 2023 (05:16:46 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202108.0486.v1

The Modified Fundamental Equations of Quantum Mechanics in Symmetric Forms

Huai-Yu Wang

Subject: Physical Sciences, Quantum Science And Technology Keywords: Dirac equation; Klein-Gordon equation; Schrödinger equation; negative kinetic energy; Decoupled Klein-Gordon equation; negative kinetic energy Schrödinger equation

Online: 25 August 2021 (10:50:21 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202202.0133.v1

Phenotypic Heterogeneity Drives Differential Disease Outcome in Triple Negative Breast Cancer

Archana P. Thankamony, Reshma Murali, Priyanka Chakraborty, Nitheesh Karthikeyan, Binitha Anu Varghese, Vishnu Sunil Jaikumar, Mohit Kumar Jolly, Radhika Nair

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Intratumoral heterogeneity; Triple negative breast cancer (TNBC); Macc1; Phenotypic heteroge-neity

Online: 9 February 2022 (10:40:54 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints201902.0156.v4

Diophantine Equations with a Finite Number of Solutions: Craig Smorynski's Theorem, Harvey Friedman's Conjecture and Minhyong Kim's Guess

Apoloniusz Tyszka

Subject: Computer Science And Mathematics, Logic Keywords: Davis-Putnam-Robinson-Matiyasevich theorem, Diophantine equation which has at most finitely many solutions in \mbox{non-negative} integers, Hilbert's Tenth Problem, Hilbert's Tenth Problem for Q, Matiyasevich's theorem, recursively enumerable set, Smorynski's theorem

Online: 1 March 2019 (12:58:53 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202201.0230.v1

Refusal Parenting Style Is Easier for Students to Perceive Non-physical Bullying in the Upper Grades of Elementary School: Mediation Effect of Negative Affect Experience and Negative Coping Style

Houyu Zhou, Qinfei Wang, Shuxu Yu, Quangquang Zheng

Subject: Social Sciences, Psychology Keywords: perceiving campus non-physical bullying; Refusal parenting style; negative affect experiences; negative coping style; mediating effect

Online: 17 January 2022 (14:09:27 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202103.0055.v1

Semi-Covariance Coefficient Analysis of Spike Proteins from SARS-CoV-2 and other Coronaviruses for Viral Evolution and Characteristics Associated with Fatality

Jun Huang, Rebecca Spencer, Wangdong Zhang

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Fractional complex moment; SARS-CoV-2; coronaviruses; spike protein sequence; Pearson correlation coefficient; semi-covariance coefficient; positive-correlative and negative-correlative domains

Online: 2 March 2021 (09:30:12 CET)

Show abstract| Download PDF| Share

Complex modeling has received significant attention in recent years and is increasingly used to explain the statistical phenomenon with increasing and decreasing fluctuations such as the similarity or difference of spike protein charge patterns of coronaviruses. Different from the existing covariance or correlation coefficient methods in traditional integer dimension construction, this study proposes a simplified novel fractional dimension derivation with the exact Excel tool algorithm. It involves the fractional center moment extension to covariance, which ends up a complex covariance coefficient that is better than the Pearson correlation coefficient, in the sense that the nonlinearity relationship can be further depicted. The spike protein sequences of coronaviruses were obtained from the GenBank and GISAID database, including the coronaviruses from pangolin, bat, canine, swine (three variants), feline, tiger, SARS-CoV-1, MERS, and SARS-CoV-2 (including the strains of Wuhan, Beijing, New York, German, and UK variant B.1.1.7) were used as the representative examples in this study. By examining the values above and below the average/mean based on the positive and negative charge patterns of the amino acid residues of the spike proteins from coronaviruses, the proposed algorithm provides deep insights into the nonlinear evolving trends of spike proteins for understanding the viral evolution and identifying the protein characteristics associated with viral fatality. The calculation results demonstrate that the complex covariance coefficient analyzed by this algorithm is capable of distinguishing the subtle nonlinear differences in the spike protein charge patterns with reference to Wuhan strain SARS-CoV-2 for which the Pearson correlation coefficient may overlook. Our analysis reveals the unique convergent (positive correlative) to divergent (negative correlative) domain center positions of each virus. The convergent or conserved region may be critical to the viral stability or viability; while the divergent region is highly variable between coronaviruses suggesting high frequency of mutations in this region. The analyses show that the conserved center region of SARS-CoV-1 spike protein is located at amino acid residues 900, but shifted to the amino acid residues 700 in MERS spike protein, and then to amino acid residues 600 in SARS-COV-2 spike protein, indicating the evolvement of the coronaviruses. Interestingly, the conserved center region of the spike protein in SARS-COV-2 variant B.1.1.7 shifted back to amino acid residues 700, suggesting this variant is more virulent than the original SARS-COV-2 strain. Another important characteristic our study reveals is that the distance between the divergent mean and the maximal divergent point in each of the viruses (MERS>SARS-CoV-1>SARS-CoV-2) is proportional to viral fatality rate. This algorithm may help to understand and analyze the evolving trends and critical characteristics of SARS-COV-2 variants, other coronaviral proteins and viruses.

Preprint REVIEW | doi:10.20944/preprints202101.0517.v1

Metabolite Transporter and Its Impact on Metabolic Engineering Approaches

Mostafa Gomaa FADL

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Gram-negative bacterial; Metabolite transporter; pathways; metabolic engineering approaches

Online: 25 January 2021 (15:49:13 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201807.0263.v1

Geometry of Chalcogenide Negative Curvature Fibers for CO₂ Laser Transmission

Chengli Wei, Curtis R. Menyuk, Jonathan Hu

Subject: Physical Sciences, Optics And Photonics Keywords: CO2 lasers; negative curvature fibers; chalcogenide glass; fiber loss; mid-IR

Online: 16 July 2018 (08:40:16 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201904.0218.v1

A Study on the Determinants of Eco-Innovation of Korean Manufacturing Firms

Cheon Yu, Yun Seop Hwang

Subject: Business, Economics And Management, Business And Management Keywords: eco-innovation; anticipated regulation; self-regulation; industry-specific characteristics; information sourcing openness; multivariate probit model; zero inflated negative binomial model

Online: 19 April 2019 (11:25:06 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201812.0314.v1

From Individual Resource to Social Capital: The Mechanism of How Political Skill Affect Job Satisfaction and Turnover Intention

Yi Li, Mengqiu Ma

Subject: Business, Economics And Management, Human Resources And Organizations Keywords: political skill; job satisfaction; turnover intention; popularity; positive affect; negative affect

Online: 26 December 2018 (12:16:31 CET)

Show abstract| Download PDF| Share

Preprint CASE REPORT | doi:10.20944/preprints201912.0170.v1

Necrotic Skin Infection by Sphingomonas Paucimobilis in Amazonia Region

Talles Henrique Pichinelli Maffei, Frederico Alberto Bussolaro, Arlei Belaus, Alexandra Prevedello, Claudine Thereza-Bussolaro

Subject: Medicine And Pharmacology, Dermatology Keywords: sphingomonas; gram-negative bacterial infection; skin disease; bacterial

Online: 12 December 2019 (10:01:35 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202203.0357.v1

Negative Electrical Conductivity Metamaterials

Edward Bormashenko

Subject: Chemistry And Materials Science, Materials Science And Technology Keywords: metamaterial; negative electrical conductivity; negative mass; Drude-Lorentz model; negative refraction

Online: 28 March 2022 (08:56:05 CEST)

Show abstract| Download PDF| Share

Working Paper REVIEW

A Systematic Review on Antimicrobial Properties of Mediterranean Wild Edible Plants: We Still Know a Little of the Whole, but it is Worth to Persevere

Giulia Cappelli, Francesca Mariani

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Wild Edible Plants; antimicrobial effect; Mediterranean plant; Gram+ bacteria; Gram- bacteria; extraction protocols; bioactive compounds; essential oils

Online: 21 July 2021 (10:05:22 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202101.0568.v1

Perceived Help-Seeking Difficulty, Barriers, Delay, and Burden in Carers of People with Suspected Dementia

Carmen KM Ng, Dara KY Leung, Xinxin Cai, Gloria HY Wong

Subject: Business, Economics And Management, Accounting And Taxation Keywords: service accessibility; dementia knowledge; affordability; carer role strain; self-criticism; negative emotions

Online: 27 January 2021 (16:15:14 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202012.0181.v2

Metamaterials Demonstrating Negative Thermal Capacity

Edward Bormashenko, Evgeny Shulzinger

Subject: Chemistry And Materials Science, Biomaterials Keywords: metamaterials; negative effective mass; negative density; negative thermal capacity; resonance.

Online: 26 January 2021 (11:36:03 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202103.0521.v1

Modulation Instability of Surface Waves in the Model with the Uniform Wind Profile

Susam Boral, Trilochan Sahoo, Yury Stepanyants

Subject: Physical Sciences, Acoustics Keywords: Two-layer fluid; shear flow; air-water interface; surface tension; vortex sheet; Kelvin-Helmholtz instability; negative-energy wave; modulation instability

Online: 22 March 2021 (11:26:38 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201908.0064.v1

On the Trends of Registered Birth and Death Rates in Nigeria: Evidence from Generalized Linear Models

Monday Osagie Adenomon, Emmanuel Chukwuma Anikweze

Subject: Computer Science And Mathematics, Probability And Statistics Keywords: birth; death; trends; generalized linear models (GLMs); poisson; negative binomial

Online: 5 August 2019 (14:36:49 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints201910.0362.v1

Trend Analysis of Tuberculosis Cases and the Effect of HIV Cases on Tuberculosis Cases in Some West African Countries Using Panel Poisson and Negative Binomial Regression Models

Monday Osagie Adenomon, Gbenga Solomom Akinyemi

Subject: Computer Science And Mathematics, Probability And Statistics Keywords: tuberculosis (TB); human immunodeficiency virus (HIV); Acquired Immune Deficiency Syndrome (AIDS); World Health Organization (WHO); panel data; poisson; negative binomial; regression

Online: 31 October 2019 (04:33:45 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202207.0409.v1

MicroRNAs within Basal-Like Signature of Quadruple Negative Breast Cancer Impact Overall Survival in African Americans

Clayton Yates, Anusha Angajala, Aliyu Muhammad, Hughley Raymond, Shakir Md Ahmed, Saadia Haleema, Monira Haque, Honghe Wang, Rachel Martini, Balasubramanian Karanam, Andrea G. Kahn, Deepa Bedi, Melissa Davis, Ming Tan, Windy Dean-Colomb

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: QNBC (Quadruple negative breast cancer); AR (Androgen receptor); AA (African American); CA (Caucasian); BC (Breast cancer)

Online: 27 July 2022 (04:07:32 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202102.0130.v1

A Novel Treatment of the Josephson Effect

Jacob Szeftel, Nicolas Sandeau, Michel Abou Ganthous

Subject: Physical Sciences, Acoustics Keywords: Josephson effect; transport properties; critical current; tunnelling; superconducting-normal transition; negative resistance

Online: 4 February 2021 (10:01:16 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202103.0570.v1

A Stochastic Condensation Mechanism for Inducing Underdispersion in Count Models

Chenangnon Frédéric Tovissodé, Romain Glele Kakai

Subject: Computer Science And Mathematics, Probability And Statistics Keywords: Erlang process; condensed distribution; probability generating function; algebraic moments; Poisson remainder distribution; negative binomial remainder distribution

Online: 23 March 2021 (12:57:21 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202202.0112.v1

Auxetic Structures from Rotating Squares

Julian Plewa, Małgorzata Płońska, Paweł Lis

Subject: Engineering, Mechanical Engineering Keywords: auxetic structures; metamaterials; negative Poisson’s ratio (NPR)

Online: 8 February 2022 (13:15:25 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202105.0195.v1

Comparative Study of Computational Models for Reducing Air Pollution Through the Generation of Negative Ions

Paola Ortiz-Grisales, Julián Patiño-Murillo, Eduardo Duque-Grisales

Subject: Engineering, Automotive Engineering Keywords: Environmental pollution; air purification; negative ion generators; particulate matter.

Online: 10 May 2021 (14:02:23 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202103.0078.v1

How Would Different Types of Negative Life Events Predict Adolescents’ Suicidal Ideation ? - an Empirical Study Based on the Western Region of China

Moye Xin, Xueyan Yang, Kun Liu

Subject: Business, Economics And Management, Accounting And Taxation Keywords: Negative life events; Suicidal ideation; Suicidalogy; Adolescents; Gender difference

Online: 4 March 2021 (15:56:04 CET)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202109.0047.v2

Clinically Translatable Approaches of Inhibiting TGF-β to Target Cancer Stem Cells in TNBC

Andrew Sulaiman, Sarah McGarry, Sai Charan Chilumula, Vishak Vinod, Rohith Kandunuri

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Triple Negative Breast Cancer; Cancer Stem Cell; TGF-β

Online: 8 September 2021 (20:53:29 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint REVIEW | doi:10.20944/preprints201910.0255.v1

Effectiveness of Efflux Pump Inhibitors as Biofilm Disruptors and Resistance Breakers in Gram-negative (ESKAPEE) Bacteria

Akif Reza, J. Mark Sutton, Khondaker Miraz Rahman

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: antimicrobial resistance; biofilm; efflux pump inhibitors; antibiotic potentiation; eskapee pathogens; gram-negative bacteria

Online: 22 October 2019 (10:22:56 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201809.0595.v1

Nanoemulsion-Enabled Oral Delivery of Novel Anticancer ω-3 Fatty Acid Derivatives

Gabriela Garrastazu Pereira, Tristan Rawling, Michele Pozzoli, Curtis Pazderka, Yongjuan Chen, Colin R. Dunstan, Michael Murray, Fabio Sonvico

Subject: Chemistry And Materials Science, Nanotechnology Keywords: nanoemulsion; oral delivery; ω-3 polyunsaturated fatty acid derivative; MDA-MB-231; triple-negative breast cancer

Online: 30 September 2018 (05:14:34 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202201.0349.v1

Spatial Distribution of Cyclograpsus cinereus Dana 1851 in Rocky Shores of Antofagasta (23º27’ S, Chile)

Patricio De los Rios-Escalante, Carlos Esse, Marco Antonio Retamal, Oscar Zuñiga, Maritza Fajardo

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: Cyclograpsus cinereus; spatial distribution; intertidal; rocky shore; negative binomial distribution.

Online: 24 January 2022 (11:48:21 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201907.0147.v1

Schizophrenia Phenomenology Revisited: Positive and Negative Symptoms are Strongly Related Reflective Manifestations of an Underlying Single Trait Indicating Overall Severity of Schizophrenia

Abbas Almulla, Hussein Al-Hakeim, Michael Maes

Subject: Medicine And Pharmacology, Psychiatry And Mental Health Keywords: deficit schizophrenia, positive symptoms, negative symptoms, inflammation, neuro-immune

Online: 10 July 2019 (11:02:41 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201811.0315.v1

Modeling Traffic Accident Occurrence at Hazardous Road Locations: A Case Study in Tunisia

Fedy Ouni, Mounir Belloumi

Subject: Business, Economics And Management, Economics Keywords: Crash counts; Hazardous Road Locations; Pearson correlation; Random effect negative binomial; Negative binomial model

Online: 13 November 2018 (13:02:16 CET)

Show abstract| Download PDF| Share

Working Paper ARTICLE

Combined Radionuclide Therapy and Immunotherapy for Treatment of Triple Negative Breast Cancer

Alyssa Vito, Stephanie Rathmann, Natalie Mercanti, Nader El-Sayes, Karen Mossman, John Valliant

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: triple negative breast cancer; immunotherapy; immune checkpoint therapy; radionuclide therapy

Online: 9 April 2021 (13:26:23 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202401.0333.v1

The nonspecific effects of COVID-19 vaccination upon non-COVID-19 all-cause mortality (NCACM) in the population of England: Effects of age, sex, COVID-19 variant, vaccination history, and time in a real-world study from Jan-21 to May-23

Rodney P Jones, Andrey Ponomarenko

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: COVID–19; vaccination; all-cause mortality; age; gender; complex system; pathogen interference; seasonality; miRNAs; negative vaccine effectiveness; nonspecific vaccine effects

Online: 4 January 2024 (07:32:40 CET)

Show abstract| Download PDF| Supplementary Files| Share

All vaccines exhibit specific and nonspecific effects. Specific effects are shown by the efficacy against the target pathogen, while nonspecific effects can be detected by the change in all-cause mortality. The real-world non-COVID-19 all-cause mortality (NCACM) data for the population of England between January 2021 and May 2023 was assessed. All vaccines administered were based on the original Wuhan antigen. Each gender and age group, along with COVID-19 variant shows its own unique NCACM vaccination benefit/disbenefit time profile. The efficacy of COVID-19 vaccination against COVID-19 disease/death per se is undisputed, however, the nonspecific outcomes of COVID–19 vaccination is far more nuanced than have been widely appreciated. This confirms an earlier study on the unanticipated nonspecific effects of influenza vaccination against all-cause winter mortality. Interestingly, a high proportion of NCACM beneficial effects occurred during the first 21 days following COVID-19 vaccination, while the worst example of increased NCACM occurred during the fourth dose of COVID-19 vaccination in 19–40-year-olds for the interval >21 days post vaccination which led to NCACM 3- to 10-times higher than in the unvaccinated. By the time of the Omicron variant, NCACM outcomes in those aged 90+ are mostly adverse. The beneficial nonspecific effects of COVID-19 vaccination increase with age and reach their maximum effect during the second week post vaccination for age groups below 50 years, rising to during the third week for those aged 70+. Finally, we discuss the mechanisms by which COVID-19 vaccination could induce wider nonspecific effects. Most of these mechanisms seem to depend on gene regulation by noncoding RNAs which also interact with mRNAs. We suggest that further international studies are required to discern the nonspecific NCACM effects of the different COVID-19 variants other than the three variants prevalent in the UK, and different types/manufacturer of COVID-19 vaccines which have been employed around the world. We can only speculate regarding the nonspecific effects of the COVID-19 vaccines administered to persons with an asymptomatic infection, and during vaccine waning. Vaccines not only need to be effective against the target pathogen, as primarily determined by antibody production, but need to minimize any unanticipated adverse nonspecific effects against all-cause mortality.

Preprint REVIEW | doi:10.20944/preprints202104.0782.v2

A Review of Electrospun Carbon Nanofiber-Based Negative Electrode Materials for Supercapacitors

Arjun Prasad Tiwari, Tanka Mukhiya, Alagan Muthurasu, Kisan Chhetri, Minju Lee, Bipeen Dahal, Prakash Chandra Lohani, Hak Yong Kim

Subject: Chemistry And Materials Science, Materials Science And Technology Keywords: electrospinning; negative electrode materials; carbon nanofibers; free-standing; supercapacitor

Online: 10 May 2021 (09:34:34 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201809.0051.v2

Unbalanced Current Sharing Control in Islanded Low Voltage Microgrids

foad najafi, Matthias Fripp

Subject: Engineering, Electrical And Electronic Engineering Keywords: distributed generation; LV microgrid; negative-sequence current; zero-sequence current; power sharing; unbalance load; voltage control

Online: 5 September 2018 (05:55:55 CEST)

Show abstract| Download PDF| Share

This paper reports a new control strategy to improve sharing of unbalanced currents in islanded LV microgrids. This technique provides fast and effective sharing of positive-, negative- and zero-sequence currents, and is the first example of zero-sequence current sharing in the literature. The controllers are designed in the stationary frame. The control structure consists of four loops; 1)~the current controller; 2)~the voltage controller; 3)~the droop controller and the 4)~negative and zero sequence current controllers. The output current is considered unknown for the controller and is added to the control system as a disturbance. The proposed controller features a high gain in fundamental and harmonic frequencies, hence a good voltage quality is obtained in the presence of unbalanced and nonlinear loads. To this aim, a proportional-resonant (PR) controller is adopted as the current controller. By using a multi-resonant controller as current controller, a unified control structure is obtained which is suitable for both grid-connected and islanded modes. The voltage controller is designed using a resonant controller so that the voltage can have low VUF and THD in the presence of unbalanced and nonlinear loads. Furthermore, in this paper droop method is applied to the control structure to share real and reactive powers. Simulation studies show that the conventional droop method cannot share the oscillatory part of the output power that is due to the presence of unbalanced loads in the microgrid. This paper relies on using zero and negative sequence virtual impedance controller to share the oscillatory part of output power. By using zero-sequence virtual impedance controller (ZSVIC) and negative-sequence virtual impedance controller (NSVIC), the zero and negative sequence currents in the microgrid are controlled and shared effectively. By compensating zero- and negative-sequence currents locally, the flow of these currents in the microgrid is minimized, and the overall power quality of the islanded LV microgrid is improved.

Working Paper ARTICLE

Prognostic Impact of Immunoglobulin Kappa C (IGKC) In Early Breast Cancer

Marcus Schmidt, Karolina Edlund, Jan G Hengstler, Anne-Sophie Heimes, Katrin Almstedt, Antje Lebrecht, Slavomir Krajnak, Marco J. Battista, Walburgis Brenner, Annette Hasenburg, Jörg Rahnenführer, Mathias Gehrmann, Pirkko-Liisa Kellokumpu-Lehtinen, Ralph M Wirtz, Heikki Joensuu

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: triple-negative breast cancer; prognosis; immune system; immunoglobulin kappa C

Online: 31 May 2021 (12:27:49 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202112.0476.v1

Comparison of social inequality in HPV vaccination among teenagers with parental reports and healthcare providers’ records in the 2019 National Immunization Survey-Teen.

Sol Seo Choi, Bongkyoo Choi

Subject: Social Sciences, Sociology Keywords: Human Papillomavirus, vaccine, social inequality, NIS-Teen, false negative, SES.

Online: 30 December 2021 (00:14:38 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202007.0527.v1

Targeting Hypoxia Sensitizes TNBC to Cisplatin and Promotes Inhibition of Both Bulk and Cancer Stem Cells

Andrew Sulaiman, Sarah McGarry, Jason Chambers, Emil Al-Kadi, Alexandra Phan, Li Li, Karan Mediratta, Jim Dimitroulakos, Christina Addison, Xuguang Li, Lisheng Wang

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Triple Negative Breast Cancer; Cancer Stem Cell; Hypoxia; EGFR; Cisplatin; PDX

Online: 22 July 2020 (11:32:30 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint REVIEW | doi:10.20944/preprints202011.0689.v1

Alternative Invasion Mechanisms and Host Immune Response to Plasmodium vivax Malaria: Trends and Future Directions

Daniel Kepple, Kareen Pestana, Junya Tomida, Abnet Abebe, Lemu Golassa, Eugenia Lo

Subject: Medicine And Pharmacology, Tropical Medicine Keywords: Plasmodium vivax; Erythrocyte Invasion Mechanisms; Duffy Negative; Africa; Immunology; Epidemiology

Online: 27 November 2020 (13:08:59 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202012.0491.v1

Biotope Impact on Fluctuating Asymmetry Manifestation in Ground beetles (Coleoptera, Carabidae)

Raisa A. Sukhodolskaya, Anatoliy A. Saveliev, Natalia I. Eremeeva, Nadezhda L. Ukhova, Tatyana A. Gordienko, Rifgat R. Shagidullin, Iraida G. Vorobyova, Igor A. Solodovnikov, Anatoliy Anciferov

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: fluctuating asymmetry; ground beetles; morphometric variation; biotope impact; species*biotope interaction; negative relationship between dimensional and meristic traits

Online: 21 December 2020 (10:16:14 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints201910.0346.v1

Factors That Enhanced Prevention of Mother-to-Child Transmission of HIV in Nasarawa State of Nigeria Using Logistic, Poisson and Negative Binomial Regression Models

Monday Osagie Adenomon, David Usman

Subject: Computer Science And Mathematics, Probability And Statistics Keywords: Prevention of Mother-to-Child transmission (PMTCT); Human Immunodeficiency Virus (HIV); Acquired Immune Deficiency Syndrome (AIDs); Poisson; negative binomial; logistic; regression

Online: 30 October 2019 (04:06:36 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202110.0255.v1

Exact Solutions for a Novel H₂ Optimal Design of Electromagnetic Tuned Mass Damper

Ge Li, Qibo Mao, Yifan Luo, Yong Wang, Lei Liu

Subject: Engineering, Mechanical Engineering Keywords: electromagnetic tuned mass damper; H2 optimization; structural vibration control; negative inductance; negative resistance.

Online: 18 October 2021 (15:37:50 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202011.0640.v1

Alteration of Bacterial Communities in Anterior Nares and Skin Sites of Patients Undergoing Arthroplasty Surgery: Analysis by 16S rRNA and Staphylococcal-Specific tuf Gene Sequencing

Søren Iversen, Thor Bech Johannesen, Anna Cäcilia Ingham, Sofie Marie Edslev, Staffan Tevell, Emeli Månsson, Åsa Nilsdotter-Augustinsson, Bo Söderquist, Marc Stegger, Paal Skytt Andersen

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Arthroplasty; prosthetic joint replacement; prosthetic joint infection; systemic antibiotic prophylaxis; Antibiotics; chlorhexidine gluconate; coagulase-negative staphylococci; tuf gene sequencing; staphylome; microbiome

Online: 25 November 2020 (12:56:25 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202212.0399.v1

Artificial Diets with Selective Restriction of Amino Acids and Very Low Levels of Lipids Induce Anticancer Activity in Mice with Metastatic Triple-Negative Breast Cancer

Emilio Guillén-Mancina, Julio José Jiménez-Alonso, José Manuel Calderón-Montaño, Víctor Jiménez-González, Patricia Díaz-Ortega, Estefanía Burgos-Morón, Miguel López-Lázaro

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Amino acids, lipids, diet, low-fat diet, cancer therapy, cancer metabolism, triple-negative breast cancer, lung cancer, melanoma, colorectal cancer, ovarian cancer, metastasis

Online: 21 December 2022 (10:05:50 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202204.0080.v1

The Effect of Negative Mass in Gravitating System

Edward Bormashenko

Subject: Physical Sciences, Applied Physics Keywords: core-spring-shell system; gravitating system; negative mass; Coulomb-like forces

Online: 8 April 2022 (11:12:34 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202111.0270.v2

Improve the Three-phase Unbalance Rate of Railway System Under 2% By Three Kinds of Special Transformer Wiring

Chien-Hsu Chen, Chin E. Lin

Subject: Engineering, Electrical And Electronic Engineering Keywords: three-phase unbalance rate (TPUR); negative phase sequence unbalance rate (NPSUR); special transformer wiring (STW); Railway System Transport Cable (RSTC); Traction Supply Substation (TSS)

Online: 14 December 2021 (15:26:32 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202206.0233.v1

Social Robots Outdo the Not-so-Social Media for Self-disclosure: Safe Machines Preferred to Unsafe Humans?

Rowling Luo, Thea Zhang, Derrick Chen, Johan F. Hoorn, Ivy Huang

Subject: Business, Economics And Management, Accounting And Taxation Keywords: social media; social robots; negative mood; self-disclosure; intervention design; anthropomorphic design

Online: 16 June 2022 (06:11:48 CEST)

Show abstract| Download PDF| Share

Preprint COMMUNICATION | doi:10.20944/preprints202207.0008.v1

Dehydro-Sulfurization Utilizing Doubly-Charged Negative Ions of Molybdenum Disulfide (MoS2), Graphene-Flake (GR-28), Armchair Carbon Nanotube (CNT 6,6), and Fullerene (C-60) as Catalysts for Depollution, Natural Resource Mining, and the Chemical Functiona

Kelvin L Suggs, Duminda K Samarakoon, Alfred Msezane

Subject: Chemistry And Materials Science, Chemical Engineering Keywords: fullerene, graphene, molybdenum disulfide, carbon nanotubes, catalysts, density functional theory, enzymes, honeybee biomimicry, drones, depollution, doubly-charged negative ions

Online: 1 July 2022 (08:12:59 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201811.0438.v1

Adiabatic Thermodyhamics

Christian Fronsdal

Subject: Physical Sciences, Fluids And Plasmas Physics Keywords: entropy; thermodynamics; conservative hydrodynamics; rotations; meniscus; planets; negative pressure

Online: 19 November 2018 (09:42:08 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202203.0297.v1

The Effect of Intratumoral Interrelation among FOXP3+ Regulatory T cells on Treatment Response and Survival in Triple-Negative Breast Cancer

Noriko Goda, Chika Nakashima, Ichiro Nagamine, Sunao Otagaki

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: regulatory T cell; tumor-infiltrating lymphocyte; neoadjuvant chemotherapy; triple-negative breast cancer

Online: 22 March 2022 (07:50:45 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201811.0436.v1

Comparing Supervised Machine Learning Strategies and Linguistic Features to Search for Very Negative Opinions

Sattam Almatarneh, Pablo Gamallo

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: sentiment analysis; opinion mining; linguistic features; classification; very negative opinions

Online: 19 November 2018 (09:35:13 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202208.0433.v1

Spiropyran/Merocyanine Amphiphile in Various Solvents: A Joint Experimental–Theoretical Approach to Photophysical Properties and Self-Assembly

Vladyslav Savchenko, Nino Lomadze, Svetlana Santer, Olga Guskova

Subject: Chemistry And Materials Science, Physical Chemistry Keywords: spiropyran/merocyanine isomerization; negative photochromism; time-resolved UV-vis measurements; molecular modeling

Online: 25 August 2022 (10:22:35 CEST)

Show abstract| Download PDF| Share

This joint experimental-theoretical work focuses on molecular and photophysical properties of the spiropyran-containing amphiphilic molecule in organic and aqueous solutions. Being dissolved in tested organic solvents, the system demonstrates positive photochromism, i.e. upon UV stimulus the colorless spiropyran form is transformed into colorful merocyanine isomer. However, aqueous solution of the amphiphile possesses a negative photochromism: the orange-red merocyanine form turns to be thermodynamically more stable in water, and both UV and vis stimuli lead to the partial or complete photobleaching of the solution. The explanation of this phenomenon is given on the basis of density functional theory calculations and classical modeling including thermodynamic integration. The simulations reveal that stabilization of merocyanine in water proceeds with the energy of ca. 70 kJ mol−1, and that the Helmholtz free energy of hydration of merocyanine form is 100 kJ mol−1 lower as compared to the behavior of SP isomer in water. The explanation of such a difference lies in the molecular properties of the merocyanine: after ring-opening reaction this molecule transforms into a zwitterionic form, as evidenced by the electrostatic potential plotted around the opened form. The presence of three charged groups on the periphery of a flat conjugated backbone stimulates the self-assembly of merocyanine molecules in water, ending up with the formation of elongated associates with stack-like building blocks, as shown in molecular dynamics simulations of the aqueous solution with the concentration above critical micelle concentration. Our quantitative evaluation of the hydrophilicity switching in spiropyran/merocyanine containing surfactants may prompt the search for new systems, including colloidal and polymeric ones, aiming at remote tuning of their morphology, which could give new promising shapes and patterns for the needs of modern nanotechnology.

Preprint ARTICLE | doi:10.20944/preprints202002.0366.v1

Energy-Momentum Tensor and Parameters in Cosmological Model

Ying-Qiu Gu

Subject: Physical Sciences, Astronomy And Astrophysics Keywords: cosmological model; energy-momentum tensor; equation of state; cosmic curvature; cosmological constant; negative pressure; dynamic analysis

Online: 25 February 2020 (07:55:12 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202010.0468.v1

Portfolio Risk Assessment under Dynamic (Equi)Correlation and Semi-Nonparametric Estimation: an Application to Cryptocurrencies

Inés Jiménez, Andrés Mora-Valencia, Trino-Manuel Ñíguez, Javier Perote

Subject: Business, Economics And Management, Accounting And Taxation Keywords: Gram-Charlier series; DCC; DECO; backtesting; cryptocurrencies

Online: 22 October 2020 (21:50:55 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202112.0219.v1

Intragenic Duplication of DMRT1 in a SRY-Negative Boy with 46,XX Disorder of Sex Development

Veronica Bertini, Fulvia Baldinotti, Nina Tyutyusheva, Camillo Rosano, Cinzia Cosini, Adelaide Maria Caligo, Diego Peroni, Angelo Valetto, Silvano Bertelloni

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: 46,XX disorder of sex development; SRY-negative male; Testis determination; DMRT1; DMRT1 duplication

Online: 14 December 2021 (07:58:55 CET)

Show abstract| Download PDF| Supplementary Files| Share

Search Results

201 articles found