Increasing levels of dissolved organic matter (DOM) in watercourses in the Northern hemisphere is mainly due to reduced acid rain, climate change, and changes in agricultural practices. However, their impacts vary in time and space. To predict how DOM respond to changes in environmental pressures we need to differentiate between allochthonous and autochthonous sources, as well as identify anthropogenic DOM. In this study we distinguish between allochthonous, autochthonous, and anthropogenic sources of DOM in a diverse watercourse network using DOM characterization tools and by assessing effects of land cover on water quality. Main sources of DOM are forests discharging allochthonous humic DOM, autochthonous fulvic DOM, and runoff from urban sites and fish farms with high levels of anthropogenic DOM rich in protein like material. Specific UV absorbency (sUVa) distinguish allochthonous DOM from autochthonous and anthropogenic DOM. Anthropogenic DOM differ from autochthonous fulvic DOM by containing elevated levels of protein like material. DOM from fishponds is distinguished from autochthonous and sewage DOM by having high sUVa. DOM characteristics thus provide valuable tools for deconvoluting the various sources of DOM allowing water resource managers to predict future trends in DOM and detect anthropogenic sources of DOM.

The production of polyurethane films from bark-derived polyols requires the complete solubility of all components in the polyurethane synthesis media. In this study, a comprehensive investigation was conducted to achieve the copolymerization of black alder bark water extract-derived polyol with isocyanate for polyurethane film synthesis in THF solution. A fractionation approach using tetrahydrofuran (THF) as a solvent was employed to dissolve the extract, followed by filtration and removal of the solvent. The resulting THF-soluble fraction comprised 62±1% of the dry weight of the alder bark extract, mainly consisting of the xyloside form of the diarylheptanoid compound oregonin, along with oligomeric flavonoids and carbohydrates. The THF-insoluble fraction was the most enriched with carbohydrate compounds, followed by the crude extract and THF-soluble fraction. Another approach was aimed at obtaining bark extractives based liquid polyols suitable for producing rigid PU foams. For this purpose, oven-dried crude black alder bark water extract was liquefied with polyethylene glycol (PEG 400). The liquefaction of black alder bark and pine bark extractives was studied under different conditions, including varying temperature ranges (130-170⁰C), catalyst concentrations (0-1,5%), and bark extract content in the mixture (15-30%). The results showed that the use of sulphuric acid as a catalyst (1-1.5%) significantly improved the solubility of both extractives, enabling the attainment of extract concentrations up to 25% at 170⁰C. However, increasing the extract content beyond a certain threshold led to incomplete solubility. It was shown that the PEG-insoluble fractions consist mainly of carbohydrate components. To increase the content of biomass in liquid polyols, the effect of glycerol additions into the liquefaction agent is under study. The findings provide insights into tailoring bark-sourced polyols for polyurethane foam production through appropriate liquefaction conditions.

Micro-nano-plastics (MNPs) are an important constituent of atmospheric aerosol. However, there is still no standard procedure for their sampling and size fractionation, which is an obstacle to the aggregation and critical analysis of results obtained by different research groups. This review focuses on the sampling and fractionation methodologies used for MNPs. Moreover, a straightforward optimized methodology for the sampling and fractionation is proposed.

Guava (Psidium guajava L.) is a common fruit tree that grows in several tropical and subtropical parts of the world. The aim of this study was to employ the use of liquid-liquid fractionation to investigative the comparative antibacterial potential of crude extracts of Guava leaves and bark against selected food isolates; Escherichia coli, Pseudomonas aeruginosa, Streptococcus pneumonia, Bacillus cereus and Staphylococcus aureus. The phytochemical analysis of the extract showed presence of tannin, phenol, flavonoid and terpenoid in all extract, while steroid and saponin were absent in some. The agar diffusion method was employed for the assessment of the sensitivity of the extracts. The ethyl acetate and aqueous fractions from the stem bark acetone extract generally showed better antimicrobial activity compared with other extracts from leaves. The extract was active both against gram positive bacteria (Bacillus cereus and Streptococcus pneumonia) and gram negative bacteria (Escherichia coli, Pseudomonas aeruginosa, , Bacillus cereus and Staphylococcus aureus) at varying zone of inhibition. The results of the study showed the potential of identifying novel antibacterial agent from P. guajava bark and leaves while optimising the potential application for treatment in traditional medicine.

Variations in geochemical signatures of fossil brachiopod shells may be due to diagenesis and/or biological processes (i.e. ‘vital effects’). Characterize them is crucial to identify reliable areas into the shell suitable to paleoclimatological studies. This investigation contributes to an in-depth understanding of geochemical variations of Gigantoproductus sp. shells (SW Spain, Serpukhovian age), which could affect to the Late Paleozoic Ice Age interpretation. Microstructural, crystallographic, cathodoluminescence and geochemical (minor and trace elements, δ18O, δ13C, and strontium isotopes) characterisation have been performed on the tertiary layer of the ventral valve, to assess the preservation state. Poorly-preserved areas exhibit microstructural and geochemical changes such as recrystallisation, fracturing and higher Mn and Fe enrichment. Moreover, these areas have higher dispersion of ⁸⁶Sr, ⁸⁷Sr, δ18O and δ13C than well-preserved areas. Three structural regions have been identified in well-preserved areas of ventral valve by differences in valve curvature and thickness, such as the umbonal, thick and thin regions. These regions have different proportions of Mg, S, Na, δ18O, and δ13C, whom are interpreted as ‘vital effects’ and probably are related with growth rate differences during shell growth. Gigantoproductus tertiary layer seems the best suitable to paleoclimatological studies because it retains the original microstructure and geochemistry.

Lignocellulosic biomass fractionation is typically performed using methods that are somehow harsh to the environment, such as in the case of the kraft pulping. In recent years, the development of new sustainable and environmentally friendly alternatives has grown significantly. Among the developed systems, bio-based solvents emerge as promising alternatives for biomass processing. Therefore, in the present work, the bio-based and renewable chemicals, levulinic acid (LA) and formic acid (FA), were combined to fractionate lignocellulosic waste (i.e., Maritime pine sawdust) and isolate lignin. Different parameters, such as LA/FA ratio, temperature, and extraction time, were optimized to boost the yield and purity of extracted lignin. The LA/FA ratio was found to be crucial regarding the superior lignin extraction from the waste biomass. Moreover, the increase in temperature and extraction time enhances the amount of extracted residue but compromises the lignin purity and reduces its molecular weight. The electron microscopy images revealed that biomass samples suffer significant structural and morphological changes, which further suggests the suitability of the new developed bio-fractionation process. Same was concluded by the FTIR analysis, in which no remaining lignin was detected in the cellulose-rich fraction. Overall, the novel combination of bio-sourced FA and LA has shown to be a very promising system for lignin extraction of high purity from biomass waste, thus contributing to extend the opportunities of lignin manipulation and valorisation into novel added-value biomaterials.

Organic wastes and by-products from several activities, including food industries, farming, and animal husbandry, are a hygiene threat when aerobically decomposed. Therefore, their manage-ment is crucial for public health. In this direction, anaerobic digestion (AD) systems may be the solution by transforming waste into energy, which may decrease the environmental impact. However, their efficacy should be carefully examined. In this innovative study, we evaluated the physicochemical and microbial characteristics of liquid digestate (LD) retrieved from organic animal wastes in northern Greece using nanofiltration. Using treatment technologies including physical (solid-liquid separation, microfiltration and nanofiltration) and biological (anaerobic digestion), heavy metals and microbial (i.e., Salmonella spp., Escherichia coli, Enterococcus faecalis) concentrations reduced and nutrients were recovered. This work sets the basis for the efficient management of liquid digestate. Our method may enable the use of treated liquid digestate for unlimited irrigation water and other industrial applications of water. Apart from the sanitation process, the recovery of nutrients for soil fertilization seems to be a more sustainable way for fu-ture agricultural practices.

Roller milling of sorghum and quinoa seeds into flour fractions (course, middle, and fine) was investigated, chemically analysed and applied in the baking of gluten-free sourdough bread. Gap settings were adjusted to 0, 5, 8 and 10 for quinoa and 3, 5, and 7 for sorghum. The fine fractions reached values of up to about 41% (gap 8) for quinoa and around 20% for sorghum (gap 5). SEM pictographs illustrated clear separation of each fraction with chemical analysis showing high contents of protein, TDF (total dietary fibre) and IDF (insoluble dietary fibre) in the course fraction. Up to 77% starch content was obtained in the fine fraction with significant amounts of SDF (soluble dietary fibre) which has good health benefits. Increasing the dough moisture up to 90% helped in decreasing the bread crumb firmness, while low Avrami parameters and RVA pasting behaviour indicated slow bread staling rate for both sourdough breads.

Marangoni flow in foam fractionation in the lamellar film for the interior and exterior of a micro-foam was investigated. The three-dimensional node-film-Plateau Border system was modeled using computational fluid dynamics. The importance of the surfactant concentration of the foam fractionation column and air-liquid interface mobility on the Marangoni velocity in the film was emphasized. The study found that an increase in surfactant concentration in the reflux column significantly increases the Marangoni velocities. Additionally, a mobile interface results in a higher Marangoni flow, while a rigid interface leads to less intensive flow. The behavior of the Marangoni flow in both interior and exterior foam was explored, revealing that the flow in exterior foam has different behavior due to the presence of the wall, which reduces the Marangoni velocity compared to interior films.

The neurovascular niche is crucial for constant blood supply and blood-brain barrier (BBB) function and is altered in a number of different neurological conditions, making this an intensely active field of research. Brain vasculature is unique for its tight association of endothelial cells with astrocytic endfeet processes. Separation of the vascular compartment by centrifugation-based methods confirmed enrichment of astrocytic endfeet processes, making it possible to study the entire vascular niche with such methods. Several centrifugation-based separation protocols are found in the literature; however, with some constraints which limit their applicability and the scope of the studies. Here, we describe and validate a protocol for physically separating the neurovascular niche from the parenchyma, which is optimized for smaller tissue quantities. Using endothelial, neuronal and astrocyte markers, we show that quantitative Western blot-based target detection can be performed of both the vascular and parenchymal fractions using as little as a single mouse brain hemisphere. Validation of our protocol in rodent stroke models by detecting changes in serum albumin signals and astrocyte activation, i.e. increased glial fibrillary acidic protein expression, between the ipsilateral and the lesion-free contralateral hemisphere demonstrates this protocol as a new way of detecting BBB breakdown and astrogliosis, respectively.

Natural deep eutectic solvents (NADES) have been shown as selective and environmentally friendly solvents for the extraction of bioactive compounds. However, studies on the solubility of low molecular weight carbohydrates (LMWC) in NADES are scarce. In this work, new solubility data of LMWC in NADES are provided and a new approach based on the use of these solvents for the efficient fractionation of bioactive carbohydrates was explored for the first time. Several mono- and disaccharides and three NADES based on choline chloride (ChCl) and different donors [2-ethylene glycol (EtG), glycerol (Gly) and ethanedioic acid dihydrate (Eth)] were considered. While degradation of carbohydrates, mainly ketoses, was detected with ChCl:Eth due to its acidic nature, ChCl:EtG and ChCl:Gly were found to be useful alternatives for selectively separating bioactive ketoses and their corresponding aldoses (e.g. lactulose/lactose and tagatose/galactose) present in equimolar binary mixtures. In addition, the usefulness of ChCl:EtG for the selective enrichment of lactulose to be used as food ingredient or nutraceutical was proven (from a 25% in the reaction mixture to a 56% in the purified sample). NADES could be used for the selective fractionation of value-added carbohydrates from interfering sugars for several applications including food science, engineering or pharmaceuticals.

Bioprospecting native Australian plants offers potential discovery of latent and novel bioactive compounds. Promising cytotoxic and antibacterial activity of methanolic extracts of Pittosporum angustifolium and Terminalia ferdinandiana led to further fractionation and isolation using our laboratory’s bioassay guided fractionation protocol. Hence, the aim of this study was to further evaluate the bioactivity of the fractions and subfractions and characterize bioactive compounds using liquid chromatography mass spectroscopy (LC-MS/MS) and gas chromatography MS (GC-MS). Compounds tentatively identified in P. angustifolium Fraction 1 using LC-ESI-QTOF-MS/MS were chlorogenic acid and/or neochlorogenic acid, bergapten, berberine, 8’-epitanegool and rosmarinic acid. GC-MS analysis data showed the presence of around 100 compounds, mainly comprising of carboxylic acids, sugars, sugar alcohols, amino acids, and monoalkylglycerols. Furthermore, the fractions obtained from T. ferdinandiana flesh extracts showed no cytotoxicity, except against HT29 cell lines, and only Fraction 2 exhibited some antibacterial activity. The reduced bioactivity observed in the T. ferdinandiana fractions could be attributed to the potential loss of synergy as compounds become separated within the fractions. As a result, further fractionation, and separation of compounds in these samples were not pursued. However, additional dose-dependent studies are warranted to validate the bioactivity of T. ferdinandiana flesh fractions, particularly since this is an understudied species. Moreover, LC-MS/GC-MS studies confirm the presence of bioactive compounds in P. angustifolium Fraction 1/subfractions which helps to explain the significant acute anti-cancer activity of this plant. The screening process designed in this study has the potential to pave the way for developing scientifically validated phytochemical/bioactivity information on ethnomedicinal plants, thereby facilitating further bioprospecting efforts, and supporting the discovery of novel drugs in modern medicine.

The authors of this paper use an original method of diatomaceous earth fractionation, which allows for obtaining a filler with a specific particle size distribution. The method makes it possible to separate small, disintegrated and broken diatom frustules from those which maintained their original form in diatomaceous earth. The study covers a range of tests conducted to prove that such a separated diatomic fraction shows features different from the base diatomite used as an epoxy resin filler. We have examined mechanical properties of a series of diatomite/resin composites considering the weight fraction of diatoms and the parameters of the composite production process. The studied composites of Epidian 601 epoxy resin cross-linked with amine-based curing agent Z-1 contained 0 to 70% vol. of diatoms or diatomaceous earth. Samples were produced by casting into silicone moulds in vacuum degassing conditions and, alternatively, without degassing. The results have shown that the size and morphology of the filler based on diatomaceous earth affects mechanical and rheological properties of systems based on epoxy resin.

Leaf crude extract (aqueous) of Oroxylum indicum (L.) Kurz induces genomic DNA fragmentation, comet formation, and inhibition of cell proliferation in prostate cancer cell line, PC3 as assessed by agarose gel electrophoresis, comet assay, and MTT assay respectively. The bioactive compound was purified through bioassay-guided fractionation using preparative HPLC and MTT as-say. The brown and water-soluble compound was characterized using 1H and 13C nuclear magnetic resonance (NMR), fourier transform infrared (FT-IR) and electrospray ionization (ESI) mass spectrometry, and the compound was iden-tified as a glycosylated hydroquinone derivative, 2-[p-(2-Carboxyhydrazino)phenoxy]-6-(hydroxymethyl) tetrahy-dro-2H-pyran-3,4,5-triol (molecular formula, C13H18N2O8; molecular mass = 330). The identified phytocompound has not been reported earlier elsewhere. Therefore, the common name of the novel anticancer phytocompound isolated from oroxylum indicum in this current study is named as oroxyquinone. The half-maximal inhibitory concentration (IC50) of oroxyquinone on PC3 cells was 19.44 µg/ml (95% CI = 17.97 to 21.04). Oroxyquinone induced cell cycle arrest at S phases and inhibition of cell migration on PC3 as assessed by flow cytome-try and wound healing assay respectively. On investigating the molecular mechanism of inducing apoptosis, the results indicated that the oroxyquinone induced apoptosis through the p38 pathway and cell cycle arrest, however, not through caspase-3 and PARP pathways. The present study identifies a novel an-ticancer molecule and provides scientific evidence supporting the therapeutic potency of OI for ethnomedicinal uses.

Tropical forests constitute prolific sanctuary of unique floral diversity and potential medicinal sources, however, many of them remains unexplored. Herein, seven Mascarene endemic plants leaves were extracted and evaluated for their in vitro antioxidant properties and antiproliferative effects on a panel of cancer cell lines using MTT and clonogenic cell survival assay. Flow cytometry and comet assay were used to investigate the cell cycle and DNA damaging effects, respectively. Bioassay guided-fractionation coupled with LC-Mass spectrometry (MS), gas chromatography-MS, and nuclear magnetic resonance spectroscopy analysis were used to identify the bioactive compounds. Among the seven plants tested, Terminalia bentzoë was comparatively the most potent antioxidant extract with significantly (p < 0.05) higher cytotoxic activities. T. bentzoë extract further selectively suppressed the growth of human hepatocellular carcinoma cells and significantly halted the cell cycle progression in G0/G1 phase, decreased the cells replicative potential and induced significant DNA damage. Ten phenolic compounds including punicalagin and ellagic acid were identified and likely contributed to the extract potent antioxidant and cytotoxic activities. These results established a promising basis for further in-depth investigations on the potential use of T. bentzoë as supportive therapy in cancer management.

The present work reports new mineralogical and whole rock geochemical data from the Breivikbotn silicocarbonatite (Seiland igneous province, North Norway), allowing conclusions to be drawn concerning its origin and the role of late fluid alteration. The rock shows a rare mineral association: calcite + pyroxene + amphibole + zeolite group minerals + garnet + titanite, with apatite, allanite, magnetite and zircon as minor and accessory minerals, and it is classified as silicocarbonatite. Calcite, titanite and pyroxene (Di36-46 Acm22-37 Hd14-21) are primarily magmatic minerals. Amphibole of hastingsitic composition has formed after pyroxene at a late-magmatic stage. Zeolite group minerals (natrolite, gonnardite, Sr-rich thomsonite-(Ca)) were formed during hydrothermal alteration of primary nepheline by fluids/solutions with high Si-Al-Ca activities. Poikilitic garnet (Ti-bearing andradite) has inclusions of all primary minerals, amphibole and zeolites, and presumably crystallized metasomatically during a late metamorphic event (Caledonian orogeny). Whole rock chemical compositions of the silicocarbonatite differs from the global average of calciocarbonatites by elevated silica, aluminium, sodium and iron, but show comparable contents of trace elements (REE, Sr, Ba). Trace element distributions indicate within-plate tectonic setting of the carbonatite. The spatial proximity of carbonatite and alkaline ultramafic rock (melteigite), the presence of “primary nepheline” in carbonatite together with the trace element distributions indicate that the carbonatite was derived from crystal fractionation of a parental carbonated foidite magma. The main prerequisites for the extensive formation of zeolite group minerals in silicocarbonatite are revealed.

In recent years, the genesis of natural gas and the definition of major gas source rocks in the Baiyun Sag of the Pearl River Mouth Basin have attracted extensive attention from scholars. There is still considerable controversy over the genesis and the main gas source rocks of natural gas. In this study, the chemical kinetics method of natural gas generation and carbon isotope fractionation was used to study the generation history and isotope evolution history of Paleogene natural gas in the Y5 well area located on the north slope of Baiyun Sag. And other geochemical discriminant methods were applied simultaneously to determine the type and source rocks of natural gas. The results show that the natural gas in the Y5 well area on the northern slope of Baiyun Sag is mainly kerogen degradation gas, mixed with a small amount of oil cracking gas. The mixture of kerogen degradation gas and a small amount of crude oil cracking gas caused the inversion of the carbon isotope sequence of ethane and propane. The natural gas in the Y5 well area mainly comes from delta–shallow lacustrine facies source rock. The high–quality reservoirs in the shallower strata accumulate natural gas generated in the early stage, and the tight reservoirs in the deeper strata mainly accumulate natural gas generated in the late stage.

Previous evidence links the formation of extranuclear inclusions of transcription factors, such as ERK, Jun, TDP-43, and REST with oxidative, endoplasmic-reticulum, proteasomal, and osmotic stress. To further characterize its extranuclear location, we performed a high-content screening based on confocal microscopy and automatized image analyses of an epithelial cell culture treated with hydrogen peroxide, thapsigargin, epoxomicin, or sorbitol at different concentrations and times to recreate the stresses mentioned above. We also performed subcellular fractionation of the brain from transgenic mice overexpressing the Q331K mutated TARDBP, and we analyzed REST-regulated mRNAs. The results show that these nuclear proteins exhibit a mitochondrial location, together with significant nuclear/extranuclear ratio changes, in a protein and stress-specific manner. The presence of these proteins in enriched mitochondrial fractions in vivo confirmed the results of image analyses. TDP-43 aggregation was associated with alteration in mRNA levels of REST target genes involved in calcium homeostasis, apoptosis, and metabolism. In conclusion, cell stress increased mitochondrial translocation of nuclear proteins, increasing the chance of proteostasis alterations. Further, TDP-43 aggregation impacts REST target genes, disclosing an exciting interaction between these two transcription factors in neurodegenerative processes.

The structure of existing activated models is inherently deficient in reflecting the major role of the membrane filtration. This study developed and proposed a novel model, MASM, for the membrane activated process. The effective filtration size imposed by the membrane module, entrapping larger size particles was adopted as the basis of the proposed model. The model defined a modified COD fractionation, accounting for the captured COD fractions as additional model components and utilizing related mass balance relationships. It was implemented for testing the fate of soluble hydrolysable COD and system performance of super-fast membrane activated sludge based on real data for the characterization and process kinetics of domestic sewage and denim processing effluents. Model evaluation was carried for parallel systems with gravity settling and membrane filtration operated at a sludge age range of 0.5-2.0 d. Results reflected significantly better performance of super-fast membrane activated sludge system for both wastewaters, underlining that it was crucially important to account for the captured COD fractions to provide an accurate evaluation of system behavior and effluent quality. This should also be identified as the major shortcoming of the ASM models for evaluating and predicting system performance of activated sludge configurations with membrane separation.