ARTICLE | doi:10.20944/preprints202011.0146.v4
Subject: Chemistry, Other Keywords: Ferric Uptake Regulation; Fur, Iron(II), Molecular dynamics; DNA; protein
Online: 10 November 2020 (12:23:58 CET)
Ferric uptake regulation protein is a repressor protein which binds an AT rich region of DNA (the iron box). Fur binds as a dimer in a helix turn helix mode and it is activated by iron(II) and other divalent transition metal ions at elevated concentrations in a process to regulate the ion uptake. Each transition metal ion induces certain conformational changes to aid the Fur binding, both the N-terminal and C-terminal domains take part in binding to DNA in addition to His 88 and His 86 residues. The process is discussed in view of experimental reports. Fe(II), Mn(II) and Co(II) activate Fur to bind DNA experimentally but Zinc plays a structural role and does not activate Fur to bind DNA.