COMMUNICATION | doi:10.20944/preprints202211.0165.v1
Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Proteomics; LC-MS/MS; phosphopeptide enrichment; bioinformatics; cellular signaling; Mag-naporthe oryzae; phosphorylation; DDA; DIA; phospho-peptidomics
Online: 9 November 2022 (02:09:17 CET)
The dynamic interplay of signaling networks in most major cellular processes is characterized by the orchestration of reversible protein phosphorylation. Consequently, analytic methods like quantitative phospho-peptidomics has been pushed forward from a highly specialized edge-technique to a powerful and versatile platform for comprehensively analyzing the phosphorylation profile of living organisms. Despite enormous progress in instrumentation and bioinformatics, a major problem remains a high number of missing values caused by the experimental procedure due to either a random phospho-peptide enrichment selectivity or borderline signal intensities, which both cause the exclusion for fragmentation using the commonly applied data dependent acquisition (DDA) mode. Consequently, an incomplete dataset reduces confidence in the subsequent statistical bioinformatic processing. Here, we successfully applied data independent acquisition (DIA) by using the filamentous fungus Magnaporthe oryzae as model organism and could prove that while maintaining data quality (such as phosphosite and peptide sequence confidence), the data completeness increases dramatically. Since the method presented here reduces the LC-MS/MS analysis from 3 h to 1 h and increases the number of phoshosites identified up to 10-fold in contrast to published studies in fungi, we pushed the phospho-proteomic technique beyond its current limits and could provide a sophisticated resource for investigation of signaling processes in filamentous fungi.
ARTICLE | doi:10.20944/preprints202306.1325.v1
Subject: Physical Sciences, Applied Physics Keywords: high performance ETL TiO2; nanocolumns; glancing angle deposition (GLAD); mag-netron sputtering (MS)
Online: 19 June 2023 (09:32:44 CEST)
The electron Transport layer (ETL) plays a crucial role in solar cell technology, particularly in perovskite solar cells (PSCs), where nanostructured TiO2 films have been investigated as superior ETL’s compared to compact TiO2. In this study we explore the nanocolumnal growth of TiO2 in the anatase phase, for bilayer thin films by magnetron sputtering (MS) technique and glancing angle deposition (GLAD). By optimizing the growth parameters, we achieved the formation of TiO2 nanocolumns with a cross-sectional diameter ranging from 50 to 75 nm. The average thickness of the films exceeded 12.71 ± 0.5µm , while the filaments exhibited an inclination angle close to 70◦ , corresponding to the deposition angle. Furthermore, we observed a correlation between the quality of the initial layer and enhanced growth of the TiO2 nanocolumns.
ARTICLE | doi:10.20944/preprints202303.0117.v1
Subject: Computer Science And Mathematics, Computational Mathematics Keywords: Bifurcation; Elastic walls; Finite element method; Stenosis; Wall shear stress; Mag-netic field
Online: 7 March 2023 (02:03:46 CET)
To investigate the impact of a magnetic field on plaque development in a stenotic bifurcated artery, a finite element method is utilized. The blood flow is modelled as a stable, incompressible, Newtonian, biomagnetic, and laminar fluid. Furthermore, the arterial wall is assumed to be linear elastic. The Arbitrary Lagrangian Eulerian (ALE) method is employed to describe the hemodynamic flow in a bifurcated artery under the influence of an asymmetric magnetic field, taking into account two-way fluid-structure interaction coupling. A stable $P_2P_1$ finite element pair discretizes a nonlinear system of partial differential equations that requires a solution. The Newton-Raphson method is utilized to find a solution to the resulting nonlinear algebraic equation system. Numerical modelling is used to simulate the presence of magnetic fields, and the resulting displacement, velocity magnitude, pressure, and wall shear stresses are shown for a range of Reynolds numbers ($Re = 500$, $1000$, $1500$, and $2000$). The results of the numerical analysis demonstrate that the presence of a magnetic field has a significant effect not only on the magnitude of displacement but also on the velocity of the flow. The application of a magnetic field reduces flow separation, extends the recirculation area near the stenosis, and increases wall shear stress.