Nowadays, the global tomato production must cope with divers challenges including a high cost of inputs, losses due to pests and diseases and unfavourable climate changes. Although coping with climate change as a plant stressor may be an area that requires multifaceted commitment, using biostimulants emerges as an interesting option. Here, we aimed to determine the effect of Nurspray®, composed by hydroxycinnamic acid oligomers, on the yield and fruit quality of tomatoes grown under open-field conditions. Different application timing was tested to define strategies that lead to improving their agronomic impact and yield. The assessments were composed by the analysis of plant development (root systems, canopy, and flowering), plant physiological status, fruit quality and yield. The results showed the great potential of Nurspray® as biostimulant, as they enhance crop growth, flowering, fruiting, yield, and yield quality, as well as promoting plant defence. Also, Nurspray® showed a good level of biostimulant effect at different plant stage being the best application strategy the use of three doses at different times: early, intermediate, and advanced stages of the crop. With great clarity, we can affirm the huge potential of Nurspray® and above all, the significant merit of being able to use this fertilizer in organic farming.

Osteosarcoma is the most common subtype of primary bone cancers, affecting mostly adolescents. In recent years, several studies have focused on elucidating the molecular mechanisms of this sarcoma; however, its molecular etiology has still not been determined with precision. Therefore, we applied a consensus strategy with the use of several bioinformatics tools to prioritize genes involved in its pathogenesis. Subsequently, we assessed the physical interactions of the previously selected genes and applied a communality analysis to this protein-protein interaction network. The consensus strategy prioritized a total list of 553 genes. Our enrichment analysis validates several studies that describe the signaling pathways PI3K/AKT and MAPK/ERK as pathogenic. The gene ontology described TP53 as a principal signal transducer that chiefly mediates processes associated with cell cycle and DNA damage response It is interesting to note that the communality analysis clusters several members involved in metastasis events such as MMP2 and MMP9 and genes associated with DNA repair complexes, like ATM, ATR, CHEK1, and RAD51. In this study, we could identify well-known pathogenic genes for osteosarcoma and prioritized genes that need to be further explored.

This study evaluates the comprehensive biological activities of anthocyanins isolated from Andean blueberry (Vaccinium floribundum Kunth) and Andean blackberry (Rubus glaucus Benth), focusing on their antimicrobial, antioxidant, antitumoral, anti-inflammatory, and hemolytic properties. Chemical characterization revealed significant anthocyanin content with complex mass spectrometric profiles indicating diverse glycosylation patterns, which might influence their bioactivity. The antimicrobial assays demonstrated that the extracts were particularly effective against Gram-positive bacteria, with minimal inhibitory concentrations (MICs) as low as 1 mg/mL for Rubus glaucus, indicating strong potential for therapeutic use. The berries' antioxidant capacity was assessed and found to be substantial, though slightly lower than that of ascorbic acid. Furthermore, the extracts exhibited notable antitumoral activities in various cancer cell lines, showing promise as adjunctive or preventative treatments against cancer. The anti-inflammatory effects were confirmed by inhibiting nitric oxide production in macrophage cells, highlighting their potential in managing inflammatory diseases. Regarding hemolytic activity, Rubus glaucus displayed dose-dependent hemolytic activity due to anthocyanins and phenolics, while Vaccinium floribundum showed no significant hemolytic activity, confirming its safety. These findings suggest that anthocyanins from Andean berries possess potent biological activities that could be harnessed for multiple health benefits in pharmaceutical and nutraceutical applications. Further studies are warranted to isolate specific bioactive compounds and explore their synergistic effects in clinical and real-world scenarios.