Semi-structured complex numbers H was a number set developed to enable division by zero in ordinary algebraic equations. Its utility has been shown in mathematics and engineering. However, very little has been done to show its usefulness in computer science. Consequently, the aim of this paper was to show the utility of semi-structured complex numbers in computer science by developing a division by zero calculator. First two computer programs were written, one for a standard (STD) calculator and the other for a division by zero (DBZ) calculator. The programs were fed 20000 randomly generated arithmetic equations of varying lengths and the space and time complexity associated with processing these equations were measured and compared to determine the efficiency of each calculator. In the process, three major contributions were made: (1) A representation for semi-structured complex numbers that enables it to be easily used by a computer was developed; (2) It was demonstrated that the DBZ calculator outperforms the STD calculator in terms of efficiency; and, (3) It was shown that the number set H reduced the amount of error handling required to run a computer program. These results provide a firm foundation to advance the number set as a useful tool in computer science.

Matrices and their inverses allow accurate calculations to be produced quickly and compactly and can be used to control any process represented by a system of equations. Singular matrices require extensive workarounds (costing time and money) to solve these systems of equations because they have no inverse. Finding their inverse involves division by zero. Nevertheless, recently a new number set called semi-structured complex numbers was developed to enable division by zero in algebraic equations. The aim of this research was to demonstrate that the inverse of a singular matrix can be found using semi-structured complex numbers. This research reveals (1) Singular matrices and their inverses can be used to find a unique solution to a pair of simultaneous equations that appear to have infinitely many solutions or appear to have no solution; (2) Singular matrices map coordinates in projective space to coordinates in Euclidean space and their inverse maps coordinates in Euclidean space to coordinates in projective space; (3) the inverse of a singular matrix proves that parallel lines in Euclidean space do not intersect but in projective space intersect at a “point at infinity” and (4) collinear lines that intersect at infinitely many points in Euclidean space only intersect at one point in projective space.

Peptidoglycan hydrolases are enzymes that cleave the peptidoglycan of the bacterial cell wall, facilitating cell growth, cell division, and peptidoglycan turnover. Xanthomonas citri subsp. citri (X. citri), the causal agent of citrus canker, encodes an Escherichia coli M23 peptidase EnvC homolog. EnvC is a LytM factor required for septal peptidoglycan splitting and daughter cell separation. In this study we investigate how EnvC contributes to the virulence and cell separation of X. citri. We observed that disruption of the X. citri envC gene (ΔenvC) led to a reduction in virulence. Upon inoculation into leaves of Rangpur lime (Citrus limonia Osbeck), the X. citri ΔenvC mutant showed a delay in causing citrus canker symptoms compared with the wild-type X. citri strain. Mutant complementation restored the X. citri wild type phenotype. Subcellular localization confirmed that X. citri EnvC is a periplasmic protein. Moreover, the X. citri ΔenvC mutant exhibited elongated cells, indicative of a cell division defect. These findings support the role of EnvC in regulating virulence, cell wall organization, and cell division in X. citri.

Extremely low-frequency electromagnetic fields (ELF-EMFs) are non-ionizing radiations typically emitted by power lines, electrical wiring and electrical appliances. Epidemiological studies have repeatedly shown a positive association between ELF-EMFs and childhood leukemia. Exposures greater than 0.3-0.4 µT increase the risk by approximately 1.5-2 fold, and estimates indicate that up to 2% of childhood leukemia cases in Europe may be attributable to ELF-EMFs. However, it is considered unlikely that ELF-EMFs can cause cancer, because carcinogenesis requires the accumulation of DNA alterations and ELF-EMFs do not have enough energy to damage the DNA. Lack of biological plausibility is a barrier to accept the evidence of carcinogenicity in human studies and to take measures to protect pregnant women and children from ELF-EMFs. Recent evidence indicates that non-mutagenic agents can cause DNA alterations and increase the risk of cancer by promoting the accumulation of cell divisions in stem cells. Cell division generates DNA alterations (e.g., mutations arising during DNA replication), which occur even in the absence of DNA-damaging agents. Importantly, ELF-EMFs can trigger the division of stem cells; this effect is under development in the field of regenerative medicine. A possible mechanism by which ELF-EMFs induce the malignant transformation of hematopoietic stem cells is discussed.

Epidemiological data indicate that 5.8% of cancer deaths world-wide are attributable to alcohol consumption. The risk of cancer is higher in tissues in closest contact on ingestion of alcohol, such as the oral cavity, pharynx and esophagus. The risk of these cancers is increased even in people who have only one alcoholic drink per day. However, since ethanol is not mutagenic and the carcinogenic metabolite of ethanol (acetaldehyde) is mostly produced in the liver, it is not clear why alcohol use preferentially exerts a local carcinogenic effect. It is well known that ethanol causes cell death at the concentrations present in alcoholic beverages; however, this effect has been overlooked probably because dead cells cannot give rise to cancer. Here I discuss that the cytotoxic effect of ethanol on the cells lining the oral cavity, pharynx and esophagus activates the division of the stem cells located in deeper layers of the mucosa to replace the dead cells. Every time stem cells divide, they become exposed to unavoidable errors associated with cell division (e.g., mutations arising during DNA replication and chromosomal alterations occurring during mitosis) and also become highly vulnerable to the genotoxic activity of endogenous and exogenous DNA-damaging agents (e.g., reactive oxygen species, acetaldehyde and tobacco carcinogens). Alcohol consumption probably increases the risk of developing cancer of the oral cavity, pharynx and esophagus by promoting the accumulation of cell divisions in the stem cells that maintain these tissues in homeostasis. Because the cytotoxic activity of ethanol is concentration-dependent, the risk of these cancers will not only increase with increasing amounts of ethanol, but also with increasing concentrations; an ounce of whisky is probably more carcinogenic when taken undiluted than when taken mixed with non-alcoholic beverages. The local cytotoxic effect of ethanol can also explain the known synergistic effect of alcohol and tobacco use on the risk of these cancers. Understanding the mechanisms of carcinogenicity of alcohol is important to reinforce the epidemiological evidence and to raise public awareness of the strong link between alcohol consumption and cancer.

COVID-19 virus has become a global threat to the human population and society. The virus which originated from China in December, 2019 has swept across continents in the world. Cameroon has been one of the countries with a high infectious rate in the recent period having a majority of the infectious cases in the Littoral region. Lack of awareness play a major role in the rise of COVID-19 cases. This paper examined the perceptions of the existence and spread of the COVID-19 virus in Wouri Division, Littoral Region of Cameroon. The study involved a qualitative survey designed by online questionnaires which were forwarded to the inhabitants of Wouri Division through e-mails and ‘WhatsApp’. The survey included the perception of the origin and existence of the COVID-19 virus, its mode of disease transmission, preventive and control measures. A total of 573 respondents were obtained (317 females and 256 males). From the results, although a greater proportion of the respondents showed knowledge towards the COVID- 19 virus existence, transmission and prevention that could lead to a reduction in the spread of the disease, our findings suggested that there’s more need for public sensitisation of the virus especially its method of transmission, control and prevention.

Cynomorium songaricum is an important endangered plant with significant medicinal and edible values. However, the lack of resources and quality variation have limited the comprehensive developments and sustainable utilization of C. songaricum. Here, we evaluated chemical and genetic traits of C. songaricum from the highly suitable habitat regions simulated with species distribution models. The PCA and NJ tree analyses displayed intraspecific variation in C. songaricum, that could be divided into two ecotypes: ecotype I and ecotype II. Furthermore, the LC-MS/MS-based metabolomic was used to identify and analyze the metabolites of two ecotypes. The results indicated that a total of 589 compounds were detected, 236 of which were significantly different between the two ecotypes. Specifically, the relative content and the kind of flavonoids were more abundant in ecotype I, which were closely associated with the medicinal activities. In contrast, amino acids and organic acids were more enriched in ecotype II, which may provide better nutritional quality and unique flavor. In summary, our findings demonstrate the ecotype division and chemical diversity of C. songaricum in China from different geographical regions and provide a reference for the development of germplasm and directed plant breeding of endangered medicinal plants.

A novel 8-channel demux device based on multicore photonic crystal fiber (PCF) structures that operate at C-band range (1530-1565nm) has been demonstrated. The PCF demux design is based on replacing some air-holes areas with lithium niobate and silicon nitride materials over the PCF axis alongside with the appropriate optimizations of the PCF structure. The beam propagation method (BPM) combined with Matlab codes were used to modeled the demux device and to optimized the geometrical parameters of the PCF structure. Simulation results show that 8-channel can be demultiplexing after light propagation of 5 cm with large bandwidth (4.03-4.69nm) and crosstalk ((-16.88)-(-15.93) dB). Thus, the proposed device has a great potential to be integrated in dense wavelength division multiplexing (DWDM) technology for increasing performances in networking systems.

Recent evidence indicates that the risk of being diagnosed with cancer in a tissue is strongly correlated (0.80) with the number of stem cell divisions accumulated by the tissue. Since cell division can generate random mutations during DNA replication, this correlation has been used to propose that cancer is largely caused by the accumulation of unavoidable mutations in driver genes. However, no correlation between the number of gene mutations and cancer risk across tissues has been reported. Because many somatic mutations in cancers originate prior to tumor initiation and the number of cell divisions occurring during tumor growth is similar among tissues, here I use whole genome sequencing information from 22,086 cancer samples and incidence data from the largest cancer registry in each continent to study the relationship between the number of gene mutations and the risk of cancer across 33 tissue types. Results show a weak positive correlation (mean = 0.14) between these two parameters in each of the five cancer registries. The correlation became stronger (mean = 0.50) when gender-related cancers were excluded. Results also show that 1,003 samples from 29 cancer types have zero mutations in genes. These data suggest that cancer etiology can be better explained by the accumulation of stem cell divisions than by the accumulation of gene mutations. Possible mechanisms by which the accumulation of cell divisions in stem cells increases the risk of cancer are discussed.

Aurora Kinase A being overexpressed in majority of cancers, appear to be an attractive therapeutic target. However, a Phase III clinical trial of Alisertib, a selective AURKA inhibitor, resulted in no better response compared to the comparator arm of chemotherapeutic regimen raising question regarding ability of the same to target the undetectable stem cell functions. In silico analysis indicated regulation of AURKA by the stemness factors Oct4 and Sox2. TCGA data indicated positive correlation of each of the factors with AURKA which were eventually validated in cell lines, patient tissues and blood by flow cytometry along with Oct4 binding on AURKA promoter being detected by ChIP assay. However, indirect immunofluorescence and cell cycle analyses indicated proliferation-independent AURKA functions during asymmetric cell division, a characteristic feature of stem cells. Thorough screening of the AURKA positive cells in patient samples denoted epithelial to mesenchymal transition and significant upregulation of Vimentin, a mesenchymal marker and ABCG2, a drug resistance marker under Oct4 or Sox2 influence. Overall, our study demonstrated combinatorial selection Oct4, Sox2, AURKA, Vimentin and ABCG2 for diagnostics and intervention of circulating breast cancer stem cells as a blood-based, cost-effective and simple approach which will be beneficial in reducing relapse.

The weakness of the dispersion-managed link combined with optical phase conjugation to compensate for optical signal distortion caused by chromatic dispersion and nonlinear Kerr effect of standard single mode fiber is its limited structural flexibility. We propose dispersion map that can simultaneously compensate for the distorted wavelength division multiplexed signal while increasing the configurational flexibility. Each residual dispersion per span (RDPS) in the former half of the proposed link is randomly determined, and in the latter half, the arrangement order of RDPS is the same as or inverted in the former half. We confirm that the dispersion maps in which the RDPS distribution pattern in the latter half is opposite to the arrangement order in the former half are more effective in compensation, and rather, the compensation effect is better than in the dispersion map of the conventional scheme. The notable result of this paper is that the increase of flexibility can be achieved through random arrangement of RDPS in the former half, and the compensation improvement can be achieved by through inverse arrangement in the latter half which make the distribution profile of each half link roughly symmetric with respect to the midway optical phase conjugator.

Silkworm ovary-derived BmN4 cells rely on chromatin-induced spindle assembly to form microtubule-based square mitotic spindles that ensure accurate segregation of holocentric chromosomes during cell division. The chromosome passenger protein Aurora B regulates chromosomal condensation and segregation, spindle assembly checkpoint, and cytokinesis; however, its role in holocentric organisms needs further clarification. This study examined the architecture and dynamics of spindle microtubules during prophase and metaphase in BmN4 cells and those with siRNA-mediated BmAurora B knockdown using immunofluorescence labelling. Anti-α-tubulin and anti-γ-tubulin antibodies revealed faint γ-tubulin signals co-localized with α-tubulin in early prophase during nuclear membrane rupture, which intensified as prophase progressed. At this stage, bright regions of α-tubulin around and on the nuclear membrane surrounding the chromatin suggested the start of microtubule assembling in the microtubule organizing centers (MTOCs). In metaphase, the number and size of γ-tubulin foci increased and were detected on both sides of the chromosomes. This resulted in a distinctive multipolar square spindle with holocentric chromosomes aligned at the metaphase plate. siRNA-mediated BmAurora B knockdown significantly reduced the γ-tubulin foci during prophase, impacting microtubule nucleation and spindle structure in metaphase. Spatiotemporal BmAurora B expression analysis provided new insights into the regulation of this mitotic kinase in silkworm larval gonads during gametogenesis. Our results suggest that BmAurora B is crucial for the formation of multipolar square spindles in holocentric insects, possibly through the activation of γ-tubulin ring complexes in multiple centrosome-like MTOCs.

The increasing amount of data transmitted through digital broadcasting and communication channels requires efficient transmission methods that make the most of limited resources such as frequency, time, and power. The layered division multiplexing (LDM) system, which is adopted by the Advanced Television Systems Committee (ATSC) 3.0 standard, is considered one of a solution for achieving efficient transmission. Additionally, the layered time-division multiplexing (LTDM) and time layered division multiplexing (TLDM) systems have been developed as well. These layered transmission systems, utilizing multiple physical layer pipes (PLPs), offer a wide range of services including mobile and rooftop TV. To compare the efficiencies of different layered transmission systems, the article introduces the concept of normalized channel capacity. This measure allows for a fair comparison among the systems by taking into account the specific channel capacity requirements of each service. By summing the normalized channel capacities, a method is proposed to select the most efficient transmission system among the four systems: LDM, LTDM, TLDM, and time division multiplexing (TDM). The Lagrange multiplier method is employed to find the parameters that maximize the sum of the normalized channel capacities in these systems. Through simulations conducted with the determined parameters, it is demonstrated that the LDM system exhibits the highest efficiency among the four systems.

Apple (Malus x domestica) fruit size is dependent on cell division and cell expansion, processes which are subsequently regulated by plant hormones such as auxins, gibberellins, and cytokinins. In this study, we investigated the role of cell division and cell expansion in apple growth and identified which of the two was more deterministic to final fruit size. Three cultivars of different sizes were selected, namely, ‘Twenty Ounce’ (large-sized), ‘Royal Gala’ (medium-sized), and ‘Crabapple’ (small-sized). Gene expression and cell size analyses were conducted over the course of two consecutive seasons. The expression patterns of three classes of genes were markedly similar across all cultivars. Two cell division markers, namely, MdCDKB2;2 and MdANT2, were discovered to be correlatively expressed as both displayed initially high expression levels, which gradually declined from the early to late stages of growth time course. For cell expansion markers, MdEXP3 was upregulated as the cells expanded, while MdARF106 was expressed in both the cell division and expansion stages. Meanwhile, the ripening related gene MdACO1 was expectedly expressed only during the ending stages associated with ripening. Interestingly, the cell measurements taken regularly from each cultivar throughout the same eperimental timespan showed that cell sizes were unaltered and remained constant from initial pollination at the zeroth Days After Pollination (DAP), to ripening at 120 Days After Full Bloom (DAFB).

Apomictic plants (reproducing via asexual seeds), unlike sexual individuals, avoid meiosis and egg cell fertilization. Consequently, apomixis is very important for fixing maternal genotypes in the next plant generations. Despite the progress in the study of apomixis, molecular and genetic regulation of the latter remains poorly understood. So far APOLLO (Aspartate Glutamate Aspartate Aspartate histidine exonuclease) is one of the very few described genes associated with apomixis in Boechera species. The centromere-specific histone H3 variant encoded by CENH3 gene is essential for cell division. Mutations in CENH3 disrupt chromosome segregation during mitosis and meiosis since the attachment of spindle microtubules to a mutated form of the CENH3 histone fails. This paper presents in silico characteristic of APOLLO and CENH3 genes, which may affect apomixis. Also, we characterize the structure of CENH3, study expression levels of APOLLO and CENH3 in gynoecium/siliques of the natural diploid apomictic and sexual Boechera species at the stages of before and after fertilization. While CENH3 was a single copy gene in all Boechera species, the APOLLO gene have several polymorphic alleles associated with sexual and apomictic reproduction in the Boechera genera. Expression of the APOLLO apo-allele during meiosis was upregulated in gynoecium of apomict B. divaricarpa downregulating after meiosis until 4th day after pollination (DAP). On the 5th DAP, expression in apomictic siliques increased again. In sexual B. stricta gynoecium and siliques APOLLO apo-allele did not express. Expression of the APOLLO sex-allele during and after meiosis in gynoecium of sexual plants was several times higher than that in apomictic gynoecium. However, after pollination the sex-allele was downregulated in sexual siliques to the level of apomicts and increased sharply on the 5th DAP, while in apomictic siliques it almost did not express. At the meiotic stage, the expression level of CENH3 in the gynoecium of apomicts was two times lower than that of the sexual Boechera, decreasing in both species after meiosis and keep remaining very low in siliques of both species for several days after artificial pollination until the 4th DAP, when the expression level raised in sexual B. stricta siliques exceeding 5 times the level in apomictic B. divaricarpa siliques. We also discuss polymorphism and phylogeny of the APOLLO and CENH3 genes.

Non-orthogonal multiple access (NOMA) has been investigated to support massive connectivity for Internet-of-things (IoT) networks. However, since most IoT devices suffer from limited power and decoding capabilities, it is not desirable to pair a large number of devices simultaneously, which encourages two-user NOMA grouping. Additionally, most existing techniques have not considered the diversity in the target QoS of IoT devices, which may lead to spectrum inefficiency. Few investigations have partially considered that issue by using an order-based power allocation (OPA) approach, where the power is allocated according to the order to the user's target throughput within a priority-based NOMA (PNOMA) group. However, this does not fully capture the effects of diversity in the values of the users' target throughputs. In this work, we handle both problems by considering a throughput-based power allocation (TPA) approach, that captures the QoS diversity, within a three-users PNOMA group as a compromise between spectral efficiency and complexity. Specifically, we investigate the performance of a time-division PNOMA (TD-PNOMA) scheme, where the transmission time is divided into two-time slots with two-users per PNOMA group. The performance of such TD-PNOMA is compared with a fully PNOMA (F-PNOMA) scheme, where the three users share the whole transmission time, in terms of the ergodic capacity under imperfect successive interference cancellation (SIC). The results reveal the superiority of TPA compared with OPA approach in both schemes, besides that the throughput of both schemes can outperform each other under imperfect SIC based on the transmit signal-to-noise ratio and the deployment scenarios.

o estudo visa explicar os efeitos diferentes entre as equações de multiplicação, soma, divisão e subtração. Nesse sentido, as equações de multiplicação e soma desenvolvem um novo elemento ou fato, enquanto isso, as equações de divisão expressar informações dentro do elemento ou fato, a subtração é a seleção de elemento ou fato Alem disso, para uma equação de multiplicação e soma resulta no novo elemento ou fato é necessario dependência de conceitos físicos específicos, sem alteração extrema, pois resultaria na alteração do elemento ou fato. vale ressaltar, a importância de valores fixos na equação para descreve a natureza específica do elemento ou fato. O estudo possibilita o entendimento das equações em aberta(sem solução) na matematica e fisica, além de compreender equações existentes. O estudo faz referência à teoria da necessidade obrigatória e teoria da diferenças entre elementos.

Ensemble fuzzing in parallel with heterogeneous fuzzers has been proposed to leverage the advantages of diverse fuzzers and improve testing efficiency. However, in current ensemble fuzzing methods, the collaboration among different fuzzers is achieved solely by synchronizing the seeds discovered by each fuzzer. This results in a high likelihood of different fuzzers choosing the same seeds and creating a large number of equivalent testcases, thus reducing overall fuzzing efficiency. Meanwhile, the existing task division method proposed by AFLTeam is highly coupled with the fuzzer specially designed for it, making it challenging to apply to ensemble fuzzing directly. So, in this paper, we proposed a callgraph-based task division method suitable for ensemble fuzzing. Firstly, we divided the target program’s callgraph into subgraphs (subtasks) balancing expected workloads. Then, we divided the global seed corpus into sub-corpora, each corresponding to a subtask, making fuzzers easily accept the subtasks. Finally, we designed synchronization mechanisms for coverage bitmaps and seeds to realize the collaborative fuzzing among different fuzzers, and a cyclic subtask scheduling strategy to fully leverage the benefits of ensemble fuzzing. We have implemented a prototype called TAEF. The evaluation results show that in the best-case scenario, our method has up to 24% more branch coverage than previous work.

ATP-dependent chromatin remodeling complexes are involved in nucleosomes sliding, eviction and/or histone variants incorporation into chromatin to facilitate several cellular and biological processes, including DNA transcription, replication and repair. The DOM/TIP60 chromatin remodeling complex of Drosophila melanogaster contains 18 subunits, including the DOMINO (DOM), an ATPase that catalyzes the ex-change of the canonical H2A with its variant (H2A.V); and TIP60, a lysine-acetyltransferase that acetylates H4, H2A and H2A.V histones. In the last decade, different experimental evidence showed that ATP-dependent chromatin remodeling factors, in addition to their role in chromatin organization, have a functional relevance in cell division. In particular, emerging studies suggested direct roles of ATP-dependent chromatin remodeling complex subunits in controlling mitosis and cytokinesis in both humans and D. melanogaster. However, little is known about their possible involvement during meiosis Meiotic chromosomes non-disjunction led to aneuploid offspring, which are often inviable/poorly viable or sterile due to gene dosage imbalance. Therefore, studying the role of DOM/TIP60 complex in D. melanogaster meiosis can provide new insights on our understanding of the molecular mechanisms underlying cell division control in gametogenesis.

This paper evaluates the determinants of decision making in relation to the production of four crops (banana, cassava, potato and sweet potato). Understanding the division of labour and decision-making in crop management may lead to designing better interventions targeted at improving efficiency in smallholder agriculture. A household quantitative survey with heads of households involving 261 women and 144 men in Burundi and 184 women and 222 men in Rwanda was conducted in 2014. Most of the decisions and labour provision during production of both cash crops (potato and banana) and food crops (sweet potato and cassava) were done jointly by men and women in male-headed households. Higher values for ‘credit access’, ‘land size’ and ‘farming as the main occupation of the household head’ increased the frequency of joint decision-making in male-headed households. A decline in the amount of farm income reduced the participation of men as decision makers. A reduction in total household income and proximity to the market was correlated with joint decision making. Gender norms also contributed to the lower participation of women in both decision-making and labour provision in banana and potato cultivation. Although a large proportion of decisions were made jointly, women perceived that men participate more in decision-making processes within the household during the production of cash crops. Increased participation by women in decision-making will require an active and practical strategy which can encourage adjustments to existing traditional gender norms that recognise men as the main decision-makers at both the household and community levels.

In order to understand the evolution of multicellularity, we must understand how and why selection favors the first steps in this process: the evolution of simple multicellular groups. Multicellularity has evolved many times in independent lineages with fundamentally different ecologies, yet no work has yet systematically examined these diverse selective drivers. Here we review recent developments in systematics, comparative biology, paleontology, synthetic biology, theory, and experimental evolution, highlighting ten selective drivers of simple multicellularity. Our survey highlights the many ecological opportunities available for simple multicellularity, and stresses the need for additional work examining how these first steps impact the subsequent evolution of complex multicellularity.

Holographic projection is a simple projection because it enlarges or reduces reconstructed images without using a zoom lens. However, one major problem associated with this projection is the deterioration of image quality as the reconstructed image enlarges. In this paper, we propose a time-division holographic projection, in which the original image is divided into blocks and the holograms of each block are calculated. Using a digital micromirror device (DMD), the holograms were projected at high speed to obtain the entire reconstructed image. However, the holograms on the DMD need to be binarized, thereby causing uneven brightness between the divided blocks. We correct this by controlling the displaying time of each hologram. Additionally, combining both the proposed and noise reduction methods, the image quality of the reconstructed image was improved. Results from the simulation and optical reconstructions show we obtained a full-color reconstruction image with reduced noise and uneven brightness.

Direct reprogramming of fibroblasts into induced cardiomyocytes (iCMs) holds a great promise for regenerative medicine and has been studied in several major directions. However, cell-cycle regulation, a fundamental biological process, has not been investigated during iCM-reprogramming. Here, our time-lapse imaging on iCMs, reprogrammed by Gata4, Mef2c, and Tbx5 (GMT) monocistronic retroviruses, revealed that iCM-reprogramming was majorly initiated at late-G1- or S-phase and nearly half of GMT-reprogrammed iCMs divided soon after reprogramming. iCMs exited cell cycle along the process of reprogramming with decreased percentage of EdU⁺/αMHC-GFP⁺ cells. S-phase synchronization post-GMT-infection could enhance cell-cycle exit of reprogrammed iCMs and yield more GFP^high iCMs, which achieved an advanced reprogramming with more expression of cardiac genes than GFP^low cells; however, S-phase synchronization didn’t enhance the polycistronic-MGT reprogramming, in which cell-cycle exit had been accelerated. In conclusion, post-infection synchronization of S-phase facilitated the early progression of GMT-reprogramming through a mechanism of enhanced cell-cycle exit.

Chromosome segregation in female germ cells and early embryonic blastomeres is known to be highly prone to errors. The resulting aneuploidy is therefore the most frequent reason for termination of early development and embryo loss in mammals. And when compatible with embryonic and foetal development, aneuploidy leads into severe developmental disorders. The main surveillance mechanism, essential for protection of cells against aneuploidy, is the Spindle Assembly Checkpoint (SAC). And although all eukaryotic cells are perhaps able to mount SAC response, it is not clear, whether this pathway is active in all cell types, including blastomeres of early embryos. In this review, we will summarize and discuss the published literature with regards to mechanisms controlling chromosome segregation in early embryos. Our conclusion is that early embryos show limited capabilities to react to chromosome segregation defects, which might, at least partially, explain the widespread problem of aneuploidy during early development.

Background: One of the most astounding discoveries of recent times is the recognition that cancer embodies a transition from a higher level of metazoan cell organization to a more foundational pre-metazoic state. This shift is steered by genes housed within the ancestral genome compartment, pervasive across all metazoan genomes, encompassing humans, and governed by a pre-metazoic gene regulatory mechanism (aGRN). This work aims to highlight the emerging field of evolutionary cancer cell biology (ECCB), which points to the deep homology between cancer and protist life cycles tracing back to the common ancestor of amoebozoans, metazoans, and fungi (AMF). The ECCB analysis reveals the essence of the non-gametogenic germline of the AMF ancestor, which serves as a blueprint for all metazoan germlines and stem cell lineages and controls the life cycle of cancer. Every germ and stem cell lineage of humans and metazoans traces its lineage back to this Urgermline, transmitting crucial processes such as asymmetric cell cycling, differentiation, stemness, and phenomena like GST and SGT (aka EMT and MET) to their subsequent evolutionary descendants. Oxygen-sensitive germline and stem cells suffer DNA-DSB due to stress and oxygen ranges reminiscent of ancestral hyperoxia, leading to cell senescence. Cells that can overcome senescence can proliferate as DSCD cells with defective symmetric cell division, paving the way for malignancy and PGCC cancers. Conclusions: Understanding cancer from its evolutionary origins may help break some of the logjams in cancer prevention and open up new therapeutic pathways.

Channel estimation is an important module to enhance the performance of orthogonal frequency division multiplexing (OFDM) systems. However, the presence of a large amount of noise in multipath fading time-varying channels significantly affects the channel estimation accuracy and thus the recovery quality of the received signals. Therefore, this paper proposes a double-threshold (DT) channel estimation method based on adaptive frame statistics (AFS). The method first adaptively determines the number of statistical frames based on the temporal correlation of the received signals, and preliminarily detects the channel structure by analyzing the distribution characteristics of multipath sampling points and noise sampling points during adjacent frames. Subsequently, a multi-frame averaging technique is used to expand the distinction between multipath and noise sampling points. Finally, the DT is designed to better recover the channel based on the preliminary detection results. Simulation results show that the proposed adaptive frame statistics-double-threshold (AFS-DT) channel estimation method is effective and has better performance compared with many existing channel estimation methods.

Almost 6% of cancers worldwide are attributable to alcohol consumption. Approximately half of them occur in tissues highly exposed to ethanol, such as the oral cavity, pharynx, upper larynx and esophagus. However, since ethanol is not mutagenic and the carcinogenic metabolite of ethanol (acetaldehyde) is mainly produced in the liver, it is unclear why alcohol consumption preferentially causes a local carcinogenic effect. We recently hypothesized that the cytotoxic activity of ethanol could explain the high risk of these cancers in alcohol users. Here we report that short-term exposures (2-3 seconds) to ethanol concentrations between 10% and 15% start to cause a marked cytotoxic effect on human epithelial keratinocytes in a concentration-dependent manner. After discussing new evidence that cancer is the end-result of the accumulation of cell divisions in stem cells, we explain why regular alcohol consumption imposes a high risk of cancer on these tissues. Briefly, the cytotoxicity of ethanol reduces the lifespan of the cells lining these tissues. The stem cells located in deeper layers need to divide more often than usual to renew the damaged epithelia. The accumulation of cell divisions in stem cells leads to the accumulation of cancer-promoting errors (e.g., mutations arising during DNA replication) that increase their risk of malignant transformation. Cell division also exposes the DNA of the stem cells to the genotoxic activity of acetaldehyde and tobacco carcinogens. We propose that choosing alcoholic beverages containing non-cytotoxic concentrations of ethanol, or diluting ethanol to non-cytotoxic concentrations, is a simple way to reduce the risk of cancer of the oral cavity, pharynx, larynx and esophagus in alcohol users. This preventive strategy may also abolish the known synergistic effect of alcohol drinking and tobacco smoking on the risk of these cancers.

Cancer is a complex and devastating disease that has engaged scientists and researchers for decades. Despite tremendous efforts, previous hypotheses about cancer development have not achieved significant breakthroughs. Evolutionary Cancer Cell Biology (ECCB) is a novel and emerging branch of oncological science that provides an evolutionary perspective on the origin of cancer. It reveals that the cancer genome evolved hundreds of millions of years ago, long before multicellular organisms such as metazoans and humans emerged. ECCB aims to unify all evolutionary insights, hypotheses, and theories into a cohesive framework. It investigates the intricate relationship between cancer genomics and ancient pre-metazoan genes that emerge when normal cells transform into cancer cells. It challenges the conventional wisdom of cancer research by suggesting that cancer could arise through intrinsic cellular mechanisms without genetic alterations and mutations. Furthermore, it postulates that somatic mutations are only secondary, downstream events in the process of oncogenesis.

Remote sensing technology provides a new way to explore the earth for geoscience applications. In the context of continuous deepening of geological research and the vigorous development of geospatial information science, 3D geological modeling technology has become a research hotspot in the intersection of earth science and information science. The traditional 3D geological modeling technology refers to underground 3D modeling using geological data. This modeling method can only display the underground scene in 3D visualization, but cannot display the surface in detail. In this study, remote sensing technology is adopted to improve the traditional 3D geological modeling method, so that geological modeling can be integrated with remote sensing image, and a new 3D geological modeling method based on remote sensing technology is developed. This method can perform integrated 3D visualization of underground and aboveground scenes, which provides a new way for disaster prevention and reduction, geological prospecting and tectonic interpretation. The new 3D geological modeling method is mainly applied in the geological field, and has made new progress in multi-source heterogeneous geological data fusion, large-scale high-precision modeling, geological grid subdivision, attribute modeling technology, remote sensing image fusion and other aspects. Taking the Ya'an area as an example, this paper makes use of the new generation of 3D geological modeling technology to carry out 3D geological modeling and visual display. The 3D visualization in Ya'an area verifies the feasibility and effectiveness of the new 3D geological modeling method based on remote sensing technology in the current data environment.

Metazoans have an elaborate and functionally segmented body. It evolves from a single cell by systematic divisions. Metazoans attain structural complexity with exquisite precision, which is a molecular mystery. The indispensable role of centrioles in cell division and ciliogenesis can shed insight into this riddle. Cell division helps in growth of the body and is a highly regulated and integrated process. Its errors cause malignancies. The cell mass is organized during organogenesis. Prior to it, the centrioles are retrieved from the cell cycle to initiate ciliogenesis. The cilia-modulated developmental signaling pathways elaborate the body plan. The secluded compartment of the cilium reduces noise during signaling and is essential for a precise body plan development. The dysfunctional centrioles and cilia can distort body plan. Thus, centriole has a dual role in growth and cellular organization. This concept review analyses the comprehensive interactome and the key domain features (like C2 domain) of molecules which connect and disarm the centriole from the cell cycle and ciliogenesis by switching on or off the essential regulators of the pathways. The concentration of these signaling pathways at the centriole reinforces the hypothesis that centriole is the molecular workstation to carve out structural design and complexity in metazoans.

Titania is one of the most comprehensively studied nanostructures due to its widespread applications in production of catalytic, gas- sensing and corrosion- resistance materials [1]. M-polynomial of nanotubes has been vastly investigated as it produces many degree-based topological indices which are numerical parameters capturing structural and chemical properties. These indices are used in the development of quantitative structure-activity relationships (QSARs) in which the biological activity and other properties of molecules are correlated with their structure like boiling point, stability, strain energy etc of chemical compounds. In this paper, we determine M-polynomials of single-walled titanium (SW TiO2) nanotubes and recover important topological degree based indices of them to theoretically judge these nanotubes. We also use Maple to plot surfaces associated to different types of single-walled titanium (SW TiO2) nanotubes.

A new optical steganography scheme is proposed that transmits a stealth optical code-division multiple-access (OCDMA) signal through a public binary phase-shift keying (BPSK) channel. Polarization beam splitters and arrayed waveguide gratings are used to implement a spectral-polarization coding (SPC) system with an incoherent optical source. We employ a Walsh–Hadamard code as the signature code of the user who wants to transmit stealth information using the system. A free space optical link applied to this system maintains the polarization states of light during propagation. The secret data is extracted using correlation detection and balanced subtraction in the OCDMA decoder of the intended receiver, and the other signal from the public channel is reduced by the OCDMA decoder. At the demodulator of the public channel, BPSK demodulation eliminates the stealth signal so that the public channel is not affected by the stealth signal. The two signals cannot interfere with each other. The results of this study show that our proposed optical steganography system is highly secure. The stealth signal can be favorably hidden in the public channel when the average source power of the stealth signal, public noise, and public signal are −5, −3, and 0 dBm, respectively.

Ethylene, a gaseous phytohormone, is emerging as a central player in the intricate web of plant developmental processes from germination to senescence under optimal and stressed conditions. It is present in different plant parts, encompassing the stems, leaves, flowers, roots, seeds, and fruits. This review aims to provide a comprehensive overview of ethylene's fascinating roles in shaping various plant growth and adaptation aspects. It intricately examines the multifaceted impact of ethylene on pivotal aspects of plant development, including but not limited to cell division and elongation, senescence, abscission, fruit and flower development, root hair formation, chloroplast maturation, and photosynthesis. This comprehensive assessment encompasses the intricate pathways of ethylene biosynthesis and signaling, providing a snapshot of the regulatory mechanisms governing ethylene production. Understanding ethylene's multifaceted functions has significant implications for agriculture, biotechnology, and our fundamental comprehension of plant biology. This review underscores the need for continued investigation into ethylene's intricate mechanisms and its potential to revolutionize plant development and crop management.

This paper proposes an active-reactive power collaborative scheduling model with cluster division for the flexible distributed energy resources (DERs) of smart building system to resolve the high complexity of centralized optimal scheduling of massive dispersed DERs in the distribution network. Specifically, the optimization objective of each cluster is to minimize the operational cost, the power loss cost, and penalty cost for the flexibility deficiency, and the second-order cone-based branch flow method is utilized to convert the power flow equations into the linearized cone constraints, reducing the nonlinearity and heavy computation burden of the scheduling model. Customized virtual battery models for the building-integrated flexible DERs are developed to aggregate the power characteristics of flexible resources while quantifying their regulation capacities with time-shifting power and energy boundaries. Moreover, a cluster division algorithm considering the module degree index based on the electrical distance and flexible balance contribution index is formulated for cluster division to achieve the information exchange and energy interaction in the distribution network with high proportion of building-integrated flexible DERs. Comparative studies have demonstrated the superior performance of the proposed methodology on the economic merits and voltage regulation.

Adaptive Sliceable-Bandwidth Variable Transceivers (S-BVTs) are key enabler for future optical networks. In particular, those based on Discrete MultiTone (DMT) modulation and Direct Detection (DD) can be considered a flexible solution suitable to address the cost efficiency requirement of optical metro networks. In this paper, we propose to use this cost-effective S-BVT option/implementation in optical metro networks adopting switching nodes based on Semiconductor Optical Amplifier (SOA) technology. Bit loading (BL) and power loading (PL) algorithms are applied to the Digital Signal Processing (DSP) modules, to maximize the performance and/or the capacity as well as enhance the flexibility and adaptability of the system. Our analysis considers switching nodes based on SOAs with and without filtering elements and fiber spans of 25 km. We present the results up to 100 km, with and without SOA-based nodes. Firstly, we analyze the adaptive BVT transmission using the Margin Adaptive (MA) BL/PL algorithm at a fixed bit rate of 28 Gb/s. The possibility of controlling the SOAs current is a key factor to face the transmission impairments due to the fiber and the filtering elements. We also analyze the system considering Rate Adaptive (RA) transmission at a fixed target BER of 3.8·10⁻³, showing that a maximum capacity above 34 Gb/s can be achieved for a single span of 25 km. Although the cascading of filtering elements still constitutes a limiting factor, we show that an improvement of the net bit rate performance can be obtained thanks to the combined use of S-BVT and SOA technology at the switching nodes, resulting in a promising approach for designing future optical metro networks.

At present more than 9 million people die of cancer every year. Simple and broad-spectrum drugs are still an urgent need for cancer patients. Recently, we proposed a new hypothesis that intracellular osmotic pressure (IOP) is the driving force of cell division, and abnormal tumor proliferation is the result of uncontrolled IOP in cells. On the one hand, aneuploidy and abnormal function of Na⁺/K⁺ pump lead to a faster rise of IOP in tumor cell than normal cells, on the other hand, abnormality of cytoskeleton assembly leads to the decrease of tolerance limit of cell membrane (TLCM) of tumor cells for resisting IOP. This hypothesis predicts: 1)Tumor cells were more intolerant to hypotonic stress than normal cells. 2) Maligancies may be sellectively killed by a suddenn increase of IOP and combined with decrease of the TLCM of tumors. Na⁺/K⁺ pump inhibitors can promotely increase the IOP of tumor cells and cytoskeleton inhibitors can dramatically lower the TLCM of tumor cells. Therefore, Na⁺/K⁺ pump and cytoskeleton inhibitors may have a synergetic effect to kill tumor cells. 3) Molecules regulating cell osmolality may be new targets for cancer treatment.

Tumor cells exhibit a resistance-associated secretory phenotype involving extracellular vesicles (EVs) and heat shock proteins (HSPs). This response occurs in response to cell stress and cancer therapeutics. HSPs are stress-responsive molecular chaperones promoting proper protein folding, while also being released from cells with EVs as well as in free form as alarmins. We have here investigated the secretory phenotype of castration-resistant prostate cancer (CRPC) cells using proteome analysis. We have also examined the roles of the key co-chaperone CDC37 in stressome release, epithelial-to-mesenchymal transition (EMT), and tumor progression. A number of HSP family members and their common receptor CD91/LRP1 were enriched at high levels in CRPC cell-derived EVs among over 700 other protein species. The small EVs (30 to 200 nm in size, potentially exosomes) were released even in a non-heated condition from the prostate cancer cells, whereas EMT-coupled release of EVs (200 to 500 nm, likely ectosomes) with associated HSP90α was increased after heat shock stress (HSS). Lactate dehydrogenase, a marker of membrane leakage/damage of cells, was also released upon HSS from the prostate cancer cells. During this stress response, intracellular CDC37 was also transcriptionally inducible by heat shock factor 1, and knockdown of CDC37 decreased EMT-coupled release of EVs. Triple knockdown of CDC37, HSP90α, and HSP90β was required for efficient reduction of the chaperone trio and to reduce tumorigenicity of the CRPC cells in vivo. Taken together, the data indicated that CDC37 and HSP90 are essential for stressome release and for tumorigenesis in resistant cancer.

This paper presents the design and measurement of an IEEE 802.11ad standard compatible RF transceiver for 60GHz wireless communication system. In addition to the traditional half-duplex (HD) mode, this work supports full-duplex (FD) operation to deliver better channel utilization and faster response time for the system. The isolation between the transmitter and receiver from the architecture design to system integration for FD operation has been fully considered. A digital self-interference cancellation (DSIC) is implemented in MATLAB to verify the FD performance. The super-heterodyne architecture with an intermediate frequency (IF) of 12GHz is designed to suppress the image frequencies without using extra filters. A flexible phase-locked loop (PLL) synthesizer provides a local oscillator (LO) frequency with a 2kHz resolution. Other than the time division duplex (TDD) mode used in the conventional 60GHz system, a wide bandwidth baseband digital variable gain amplifier (DVGA) with a 3dB bandwidth of more than 4GHz also supports frequency division duplex (FDD) operation. The transceiver chip is fabricated in Tower Jazz 0.18µm SiGe BiCMOS process. With on-board antenna, the transceiver covers all four channels in 802.11ad standard with MCS-12 (7.04Gbps under 1.76GSym/s and 16-QAM) under 1.5m. In the proposed system design, the RF frontend based self-interference (SI) suppression from the local transmitter to receiver LNA is around 54dB. To achieve a practical FD application, the SI is further suppressed with the help of digital SI compensation. The measured power consumption for transmitter and receiver configuration is 194mW and 231mW, respectively, in HD mode and 398mW for FDD or FD operation mode.

Spinors are used for the computation of probability in quantum mechanics. They are treated as elements of a complex vector space in contrast to a real vector space. Spinor theory is abstract mathematics with an ambiguous interpretation. The overall phase of spinors does not affect the computation of probability in quantum mechanics. If two spinors have an overall phase of imaginary number i, then they can be treated like vectors in a real vector space. The square of the magnitude of the number, which is the sum of dot product and the term having basis vector i of cross product of two such vectors, is equal to the probability computed using the corresponding spinors. Therefore, the geometry of such spinors can be easily depicted in a three-dimensional space like vectors in a real vector space. Spinors are not isotropic vectors in Hilbert space. The sum of dot product and cross product of two complex numbers is equivalent to the quotient of the division of one complex number by another. Similarly, the sum of dot product and cross product of two vectors is the quotient of the division of one vector by another. Using the rules of division of vectors, we can find the rules of multiplication of vectors. The rules of multiplication and division of basis vector i match the rules of multiplication and division of imaginary number i. Therefore, basis vector i is nothing but imaginary number i. Multiplication of dot and cross product of two vectors to the second vector will give us the components of the first vector that are parallel and orthogonal to the second vector. Vectors are also made up of complex numbers like spinors. The reason for finding dot product of complex numbers in the process of computing the probability is to ignore the overall phase in contrast to the phase difference. This is misconstrued as complex vector space in quantum mechanics. 3-D number which is nothing but a spinor with new notation is an extension of vector algebra. It can have a real number as a term in addition to the terms i, j and k. In all other respects, it is a vector. 3-D numbers are part of the number system like real numbers and complex numbers. The real numbers are one dimensional numbers, the complex numbers are two-dimensional numbers and the 3-D numbers as well as vectors and spinors are three-dimensional numbers. As polarisation and spin are one and the same, we can straight away apply 3-D numbers to polarisation of light. 3-D numbers will greatly simplify the study of areas of science where three-dimensional space is involved.