ARTICLE | doi:10.20944/preprints202203.0075.v1
Subject: Life Sciences, Biotechnology Keywords: Algae; wet anaerobic storage; dry matter loss; organic acid; lactic acid bacteria
Online: 4 March 2022 (08:45:40 CET)
Wet anaerobic storage of algal biomass is a promising preservation approach which can ensure continuous supply of these feedstocks to biorefineries year-round. An effective solution to preservation must ensure minimal dry matter loss and change in biochemical composition during storage. Therefore, the objective of this study was to investigate the preservation of Nannochloropsis gaditana biomass through wet anaerobic storage and its impact on biomass quality. Prior to storage, algae sample was inoculated with two different strains of lactic acid bacteria and thereafter stored for 30 and 180 days. Each inoculant limited dry matter loss to <10% (dry basis) after the storage durations. Final pH values (4.3 - 4.8) indicate that the biomass samples were properly ensiled, achieving the acidic conditions necessary for preservation. Compositional analysis of the biomass after storage showed a reduction in carbohydrate content, a relative increase in lipid content, and no significant change to the protein fraction. Glucose and galactose were the prevalent sugar monomers. The low dry matter loss and minimal compositional change indicates that wet anaerobic storage is an effective means of preserving algal biomass, ensuring a constant supply of algal biomass feedstock to a biorefinery.