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Preprint ARTICLE | doi:10.20944/preprints202307.1615.v1

Rectal Biopsy for Hirschsprung’s Disease: A Multicentre Study Involving Biopsy Technique, Pathology and Complications

Gil Vervloet, Antoine De Backer, Stijn Heyman, Paul Leyman, Sebastiaan Van Cauwenberge, Kim Vanderlinden, Charlotte Vercauteren, Dirk Vervloessem, Marc Miserez

Subject: Medicine And Pharmacology, Surgery Keywords: Hirschsprung’s disease; rectal biopsy; suction biopsy; punch biopsy; pathology hirschsprung

Online: 24 July 2023 (11:40:58 CEST)

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Preprint REVIEW | doi:10.20944/preprints202404.0358.v1

Neurosurgical Intervention for Nerve and Muscle Biopsies

Ali Ahmed Mohamed, Thomas Caussat, Edwin Mouhawasse, Rifa Ali, Phillip Mitchell Johansen, Brandon Lucke-Wold

Subject: Medicine And Pharmacology, Surgery Keywords: Nerve biopsy; neurosurgical biopsy; neuropathy; neuropathology; technique

Online: 4 April 2024 (10:11:25 CEST)

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Preprint TECHNICAL NOTE | doi:10.20944/preprints202306.0210.v1

Percutaneous superimposed O-arm -MRI Navigated Biopsy for Spinal Column Pathologies

Mohammad Walid Al-Smadi, István Kozma, Siran Aslan, Balázs Bölöni, Árpád Viola

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Percutaneous spinal biopsy; Imaging-guided biopsy; O-arm CT navigation; Spinal tumors; Diagnostic accuracy; Biopsy efficiency; O-arm CT-MRI fusion

Online: 2 June 2023 (14:11:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202312.0728.v1

Cell‐Free DNA as a Biomarker at Diagnosis and Follow up in 256 B and T Cell Lymphomas

Ramón Diez-Feijoo, Marcio Andrade-Campos, Joan Gibert, Blanca Sánchez-González, Lierni Fernández-Ibarrondo, Concepción Fernández-Rodríguez, Nieves Garcia-Gisbert, Laura Camacho, Marta Lafuente, Ivonne Vázquez, Lluís Colomo, Antonio Salar, Beatriz Bellosillo

Subject: Medicine And Pharmacology, Hematology Keywords: cfDNA; Liquid biopsy; Lymphoma; monitoring

Online: 11 December 2023 (15:59:03 CET)

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Preprint REVIEW | doi:10.20944/preprints202306.1940.v1

Liquid Biopsy in Neurological Diseases

Sunny Malhotra, Mari Carmen Martín Miras, Agustín Pappolla, Xavier Montalban, Manuel Comabella

Subject: Biology And Life Sciences, Life Sciences Keywords: microRNA; liquid biopsy; neurological diseases

Online: 28 June 2023 (03:26:41 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202003.0451.v2

Potential for COVID-19 Coronavirus (SARS-CoV-2) Exposure During Laparoscopic Gynaecological Surgery

Graham Taylor, Claire Shannon-Lowe, Heather Long, Sudha Sundar

Subject: Medicine And Pharmacology, Obstetrics And Gynaecology Keywords: gynaecology; gynecology infection; safety; polypectomy; biopsy

Online: 9 April 2020 (10:01:17 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.0063.v1

A Comprehensive Guide to Artificial Intelligence in Endoscopic Ultrasound

Kareem Khalaf, Maria Terrin, Manol Jovani, Tommy Rizkala, Marco Spadaccini, Katarzyna M. Pawlak, Matteo Colombo, Marta Andreozzi, Alessandro Fugazza, Antonio Facciorusso, Fabio Grizzi, Cesare Hassan, Alessandro Repici, Silvia Carrara

Subject: Medicine And Pharmacology, Gastroenterology And Hepatology Keywords: Endoscopic Ultrasound; Artificial Intelligence; Biopsy; Pathological Diagnosis.

Online: 2 May 2023 (06:05:02 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0535.v1

Circulating Anti-GB3 Antibody as Biomarker of Myocardial Inflammation in Patients with Fabry Disease Cardiomyopathy

Andrea Frustaci, Romina Verardo, Michele Magnocavallo, Rossella Scialla, Luigi Sansone, Matteo Antonio Russo

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: Fabry Disease, Myocarditis, globotriaosylceramide, inflammation, cardiac biopsy.

Online: 19 April 2023 (04:01:57 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202104.0446.v1

Short-Term Ex Vivo Culture of CTCs From Advance Breast Cancer Patients: Clinical Implications

Nuria Carmona-Ule, Miriam González-Conde, Carmen Abuín, Juan F Cueva, Patricia Palacios, Rafael López-López, Clotilde Costa, Ana B Dávila-Ibañez

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: CTC, cell culture, liquid biopsy, breast cancer

Online: 16 April 2021 (13:29:37 CEST)

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Working Paper COMMUNICATION

The Predictive Value of a Blood-Based RNA Signature for the Gemcitabine Response in Advanced Pancreatic Cancer

David Piquemal, Florian Noguier, Fabien Pierrat, Roman Bruno, Jerome Cros

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Pancreatic cancer; Predictive diagnosis; Liquid biopsy; gemcitabine

Online: 21 October 2020 (11:53:07 CEST)

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Preprint REVIEW | doi:10.20944/preprints202006.0263.v1

ctDNA as a Cancer Biomarker: A Broad Overview

Luciana Santos Pessoa, Manoela Heringer, Valéria Pereira Ferrer

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: biomarker; blood/plasma; cancer; ctDNA; liquid biopsy

Online: 21 June 2020 (11:22:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.0240.v1

An Investigative Analysis of Therapeutic Strategies in Hepatocellular Carcinoma: A Retrospective Examination of 23 Biopsy-Confirmed Cases Emphasizing the Significance of Histopathological Insights

Mugur Cristian Grasu, Radu Dumitru, Anca Zgura, Letitia Toma, Laura Iliescu, Cosmin Baciu

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: cancer; hepatocellular; biopsy; alpha-fetoprotein; immune checkpoint inhibitor

Online: 5 March 2024 (05:43:36 CET)

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Background: The Liver Imaging Reporting and Data System (LI-RADS) combines standardized terminology with a classification system for imaging findings in patients with HCC, therefore rendering diagnostic biopsy unnecessary in many cases. This retrospective study included 23 patients with a biopsy diagnosis of HCC, performed either before or after local interventional procedures, in order to evaluate the histopathologic changes induced by previous procedures and their potential influence on the response to immune therapy. Material and Methods: The study encompassed a cohort of patients diagnosed with Hepatocellular Carcinoma (HCC). Diagnosis was established through contrast-enhanced computer tomography or magnetic resonance imaging that identified LI-RADS-5 nodules in conjunction with historical liver disease and elevated alpha-fetoprotein (AFP) levels, or through histological examination confirming positivity for glypican3, heat shock protein70, and glutamine synthetase. The study detailed the liver disease etiology, LI-RADS scores, characteristics and dimensions of HCC nodules, serum AFP concentrations, Edmondson-Steiner grading, and the expression of programmed cell death ligand 1 (PD-L1) in the tumor cells. Results: Among the study's cohort of Hepatocellular Carcinoma (HCC) patients, a portion had not received any prior treatments, while the remainder experienced local HCC recurrence following trans-arterial chemoembolization or radiofrequency ablation. Observations indicated elevated alpha-fetoprotein (AFP) levels in those who had not undergone any previous interventions, showing statistical significance. The Edmondson-Steiner classification predominantly identified grade III differentiation across patients, irrespective of their treatment history. Furthermore, an increase in intra-tumoral programmed cell death ligand 1 (PD-L1) expression was noted in patients who had not been subjected to previous therapies. Conclusion: Liver biopsy offers valuable insights for patients with Hepatocellular Carcinoma (HCC), assisting in the tailoring of immune therapy strategies, particularly in cases of recurrence following prior local interventions.

Preprint CASE REPORT | doi:10.20944/preprints202310.0205.v1

A Rare Case of Isolated Hepatocellular Carcinoma Metastasis in Left Mandible in a Patient with Hepatitis C Virus Liver Cirrhosis Diagnosed after the onset of COVID 19 Infection

Dragan Mašulović, Aleksa Igić, Aleksandar Filipović, Miloš Zakošek, Dušan Bulatović, Ksenija Mijović, Marjan Micev, Danijel Galun

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: hepatocellular carcinoma; mandible metastasis; biopsy; diagnosis; Batson plexus

Online: 4 October 2023 (12:17:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.1749.v1

TFF1—A New Biomarker in Liquid Biopsies of Retinoblastoma under Therapy

Maike Anna Busch, André Haase, Emily Alefeld, Eva Biewald, Leyla Jabbarli, Nicole Duenker

Subject: Biology And Life Sciences, Other Keywords: retinoblastoma; TFF1; aqueous humor; liquid biopsy; therapy monitoring

Online: 24 August 2023 (12:33:18 CEST)

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Preprint BRIEF REPORT | doi:10.20944/preprints202306.1358.v1

Plasma Cell-free RNA PD-L1 Compared to Tissue PD-L1 Protein Expression and Outcomes with First-line Immunotherapy in Metastatic Non-small Cell Lung Cancer

Paul R. Walker, Sriraksha Jayananda, Melisa Pasli, Mahvish Muzaffar

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Liquid biopsy; PD-L1; plasma cfRNA; immunotherapy; NSCLC

Online: 19 June 2023 (12:57:52 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0313.v1

Epidermal growth factor receptor T790M Mutation Testing in Non-small Cell Lung Cancer: An International Collaborative Study to Assess Molecular EGFR T790M Testing in Liquid Biopsy

Martin Filipits, Verena Kainz, Viktor Sebek, Herwig Zach

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Liquid biopsy; EGFR; NSCLC; T790M mutation; NGS; PCR

Online: 5 June 2023 (11:06:11 CEST)

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Background: The detection of the EGFR T790M (T790M) mutation in non-small cell lung cancer (NSCLC) patients who progressed under treatment with first- or second-generation EGFR-tyrosine kinase inhibitors (TKIs) is important to offer a subsequent therapy with a third-generation EGFR-TKI. Liquid biopsy is a powerful tool to determine the T790M mutation status. Several liquid biopsy platforms with varying degrees of accuracy are available to test for T790M mutations and sensitivities may differ among these methods. Methods: As no standard exists for the testing of T790M mutation in liquid biopsy, we performed a collaborative study to describe and compare the sensitivity of different in-house liquid biopsy platforms for the detection of the T790M mutation, EGFR exon 19 deletion (del19) and EGFR L858R mutation (L858R) across multiple participating laboratories in seven Central and Eastern European countries. Results: Of the 25 invited laboratories across Central and Eastern Europe, 21 centers participated and received 10 plasma samples spiked with cell-line DNA containing the T790M, del19, or L858R mutation in different concentrations. In-house PCR-based and NGS-based methods were used accordingly, and results reported as in routine clinical practice. Two laboratories, which used the AmoyDx® EGFR 29 Mutations Detection Kit (AmoyDx) with Cobas® cfDNA Sample Preparation Kit and QX200 Droplet Digital PCR (ddPCR) with the QIAamp Circulating Nucleic Acid Kit identified all ten samples correctly. Cobas® EGFR Mutation Test v2 (Cobas), the NGS methods, and the IdyllaTM detection method used in this study performed within the known sensitivity range of each detection method. Conclusions: If a negative result was obtained from methods with lower sensitivity (e.g. Cobas), repeated liquid biopsy testing and/or tissue biopsy analysis should be performed whenever possible, with the aim of identifying T790M-positive patients to allow them to receive the optimal second-line treatment with a third-generation EGFR TKI.

Preprint ARTICLE | doi:10.20944/preprints202304.0404.v1

Circulating Tumor DNA (ctDNA) Methylation Quantification As Diagnostic Biomarker of Pheochromocytomas (PCCs) and Paragangliomas (PGLs)

Fatemeh Khatami, Leonardo Oliveira Reis, Mehdi Ebrahimi, Shirzad Nasiri, Seyed Mohammad Tavangar, Mahin Ahmadi Pishkuhi, Gita Shafiei, Ramin Heshmat, Seyed Mohammad Kazem Aghamir

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: ctDNA; liquid biopsy; Methylation; SDHCa; RDBPb; Pheochromocytoma; Paraganglioma

Online: 17 April 2023 (04:54:45 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202203.0018.v1

Investigating the Potential Use of Chemical Biopsy Devices to Characterize Brain Tumor Lipidomes

Joanna Bogusiewicz, Bogumiła Kupcewicz, Paulina Zofia Goryńska, Karol Jaroch, Krzysztof Goryński, Marcin Birski, Jacek Furtak, Dariusz Paczkowski, Marek Harat, Barbara Bojko

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: brain tumor; glioma; SPME; heterogeneity; lipids; chemical biopsy

Online: 1 March 2022 (11:13:07 CET)

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Preprint ARTICLE | doi:10.20944/preprints202111.0246.v1

Cancer Diagnosis of Microscopic Biopsy Images Using Social Spider Optimization Tuned Neural Network

Prasanalakshmi Balaji, Kumarappan Chidambaram

Subject: Computer Science And Mathematics, Computer Vision And Graphics Keywords: biopsy; cancer diagnosis; predictive models; neural network; optimization

Online: 15 November 2021 (10:40:52 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.0021.v1

Ex Vivo Fluorescence Confocal Microscopy of MRI-Guided Targeted Prostate Biopsies for Rapid Detection of Clinically Sig-Nificant Carcinomas – a Feasibility Study.

Ulf Titze, Barbara Titze, Torsten Hansen, Peter J. Barth, Furat Abd Ali, Fried Schneider, Matthias Benndorf, Karl-Dietrich Sievert

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Confocal microscopy; prostate cancer; prostate MRI; Targeted prostate biopsy

Online: 1 February 2024 (08:27:09 CET)

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Preprint ARTICLE | doi:10.20944/preprints202310.1381.v1

Patient Dose Estimation in Computed Tomography Guided Biopsy Procedures

Evagellia Siomou, Dimitrios Filippiadis, Efstathios P. Efstathopoulos, John Antonakos, Georgios S. Panayiotakis

Subject: Physical Sciences, Radiation And Radiography Keywords: patient dose; CT guided biopsy procedures; Diagnostic Reference Levels

Online: 21 October 2023 (17:40:38 CEST)

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Preprint REVIEW | doi:10.20944/preprints202309.1432.v1

Adding Value to Liquid Biopsy for Brain Tumors: The Role of Imaging

Nastaran Khalili, Hossein Shooli, Nastaran Hosseini, Anahita Fathi Kazerooni, Ariana Familiar, Sina Bagheri, Hannah Anderson, Stephen J. Bagley, Ali Nabavizadeh

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Imaging; Liquid biopsy; glioma; glioblastoma; focused ultrasound; tumor biomarkers

Online: 21 September 2023 (07:24:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0494.v1

The Clinical and Pathological Characteristics of Neonatal Cholestasis; A Case of Hevi Pediatrics Teaching Hospital in Duhok Province, Iraq

Nizar B Yahya, Ali A Ramadhan

Subject: Medicine And Pharmacology, Gastroenterology And Hepatology Keywords: Neonatal Cholestasis; Liver biopsy; Biliary atresia; triangular cord sing

Online: 7 June 2023 (05:46:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0348.v1

Core Needle Biopsy Guidance Based on Tissue Morphology Assessment With AI-OCT Imaging

Gopi Maguluri, John Grimble, Aliana Caron, Ge Zhu, Savitri Krishnamurthy, Amanda McWatters, Gillian Beamer, Seung-Yi Lee, Nicusor Iftimia

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: Tissue biopsy guidance; Optical coherence tomography imaging; Artificial intelligence

Online: 5 June 2023 (16:17:17 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0397.v1

Droplet Digital PCR Development To Quantify the DNA Meth-Ylation Levels of SEPT9 and SHOX2 in Plasma From Patients With Head and Neck Squamous Cell Carcinoma

Ilaria Grossi, Claudia Assoni, Luigi Lorini, Davide Smussi, Cristina Gurizzan, Salvatore Grisanti, Alberto Paderno, Davide Mattavelli, Cesare Piazza, Iulia Andreea Pelisenco, Giuseppina De Petro, Alessandro Salvi, Paolo Bossi

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: liquid biopsy; ddPCR; DNA methylation; cell free DNA; HNSCC

Online: 6 May 2023 (08:59:09 CEST)

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Preprint REVIEW | doi:10.20944/preprints202104.0667.v1

Liquid Biopsy: A New Tool for Overcoming CDKi Resistance Mechanisms in Luminal Metastatic Breast Cancer

Miriam González-Conde, Celso Yanez, Rafael López-López, Clotilde Costa

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: breast cancer; CDK inhibitors; liquid biopsy; resistance mechanisms; therapy

Online: 26 April 2021 (12:57:01 CEST)

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Preprint REVIEW | doi:10.20944/preprints202103.0063.v1

Liquid Biopsy: New Tool to Overcome CDKi Resistance Mechanism in Luminal Metastatic Breast Cancer

Miriam González-Conde, Celso Yanez, Rafael López-López, Clotilde Costa

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: breast cancer, CDK inhibitors, liquid biopsy, resistance mechanisms, therapy

Online: 2 March 2021 (09:53:38 CET)

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Preprint ARTICLE | doi:10.20944/preprints202003.0311.v1

Pathological Study of the 2019 Novel Coronavirus Disease (COVID-19) through Post-Mortem Core Biopsies

Sufang Tian, Yong Xiong, Huan Liu, Li Niu, Jianchun Guo, Meiyan Liao, Shu-Yuan Xiao

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: SARS-CoV-2; COVID-19; pathology; post-mortem biopsy

Online: 20 March 2020 (09:24:10 CET)

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Preprint ARTICLE | doi:10.20944/preprints201809.0342.v1

Origami Needle Guide for CT-Guided Interventions

Austin Taylor, Sheng Xu, Bradford J. Wood, Zion Tsz Ho Tse

Subject: Medicine And Pharmacology, Surgery Keywords: origami; percutaneous biopsy; computed tomography; radiologic phantom; 3D printing

Online: 18 September 2018 (10:32:24 CEST)

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Preprint REVIEW | doi:10.20944/preprints201706.0059.v1

Pathology of Adult and Paediatric Mitochondrial Myopathies

Rahul Phadke

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: mitochondrial; muscle biopsy; ragged red; COX-negative; subsarcolemmal; immunohistochemistry

Online: 13 June 2017 (06:23:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202212.0400.v1

Estimation of ALU Repetitive Element in Plasma as a Cost-Effective Liquid Biopsy Tool for Disease Prognosis in Breast Cancer

Madhumathy G Nair, Rakesh S Ramesh, Chandrakala M, Apoorva D, Snijesh VP, Vishakha Ramamurthy, Vidya M Somashekaraiah, Anupama CE, Kiruthiga Raghunathan, Anuradha Panigrahi, Manjula Das, Sujan K. Dhar, Jyothi S Prabhu

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Breast Cancer; Liquid biopsy; ctDNA; ALU 247; Disease progression; Prognosis

Online: 21 December 2022 (11:43:52 CET)

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Background: Liquid biopsy is widely recognised as an efficient diagnostic method in oncology for disease detection and monitoring. Though examination of circulating tumor cell (CTC) is mostly implemented for assessment of genomic aberrations, need of complex methodologies for their detection has impeded its acceptance in low resource settings. We evaluated the cell free DNA (cfDNA) as a liquid biopsy tool and investigated its utility in breast cancer patients. Methods: Total cell free DNA was extracted from plasma of breast cancer patients (n=167) with median follow up of more than 5 years, at various stages of the disease. Quantitative PCR was performed to estimate the copy numbers of two fractions of ALU repetitive elements (ALU 115 and ALU 247) and DNA Integrity (DI) was calculated as the ratio of ALU 247/115. Mutations in TP53 and PIK3CA in the cfDNA was estimated by next-gen sequencing (NGS) in a subset of samples. Association of the levels of both the ALU fragments with various clinico-pathological factors and disease-free survival at various stages were examined. Nomogram models were constructed with clinical variables and ALU 247 levels to predict disease-free survival and best performing model was evaluated by decision curve analysis. Results: DI and ALU 247 levels were significantly lower (p<0.0001) in the post-operative plasma when compared to their pre-surgery levels. DI and ALU 247 were found to be significantly higher in patients with metastasis (p<0.05). Patients with higher levels of ALU 247 in their post-operative plasma had significantly poor disease-free survival (p=0.005). Higher level of ALU 247 in circulation also correlated with low tumor-infiltrating lymphocytes (TIL) within their primary tumors in the ER-negative breast cancer subtype (p=0.01). Cox proportional hazard analysis confirmed ALU 247 as an independent variable of disease-free survival both in univariate and multivariate analysis [HR 1.3 (95% CI 1.047 to 1.613, p= 0.017)]. Nomogram model showed addition of ALU 247 with other variables significantly improved (C-index-0.823) the predictive ability of the model. Conclusion: Our results confirm the utility of cfDNA as an evolving liquid biopsy tool for molecular analysis. Evaluation of larger fragment of cfDNA estimated through ALU 247 can provide vital information concurrent with the pathological process of disease evolution in breast cancer and warrants expansion to other cancer types.

Preprint REVIEW | doi:10.20944/preprints202203.0273.v1

Alternatives for MRI in Prostate Cancer Diagnostics – Review of Current Ultrasound-Based Techniques

Adam Gurwin, Kamil Kowalczyk, Klaudia Knecht-Gurwin, Paweł Stelmach, Łukasz Nowak, Wojciech Krajewski, Tomasz Szydełko, Bartosz Małkiewicz

Subject: Medicine And Pharmacology, Urology And Nephrology Keywords: prostate cancer; ultrasonography; biopsy; micro-ultrasound; elastography; contrast-enhanced ultrasound

Online: 21 March 2022 (04:26:46 CET)

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Preprint REVIEW | doi:10.20944/preprints202108.0518.v2

Nucleic Acid Liquid Biopsies in Alzheimer’s Disease: Current State, Challenges, and Opportunities

Tabea M. Soelter, Jordan H. Whitlock, Avery S. Williams, Andrew A. Hardigan, Brittany N. Lasseigne

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: liquid biopsy; circulating biomarkers; Alzheimer’s disease; neurodegeneration; cell-free; diagnosis

Online: 8 March 2022 (09:56:01 CET)

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Preprint REVIEW | doi:10.20944/preprints202107.0382.v1

Microfluidic Surgery in Single Cells and Multicellular Systems

Kevin Zhang, Ambika Nadkarni, Rajorshi Paul, Adrian Martin, Sindy Tang

Subject: Medicine And Pharmacology, Surgery Keywords: Microfluidics; surgery; single cell; multicellular systems; sectioning; ablation; biopsy; fusion

Online: 16 July 2021 (14:53:11 CEST)

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Working Paper REVIEW

Commercial and Emerging Technologies for Cancer Diagnosis and Prognosis Based on Exosomal Biomarkers

Nareg Ohannesian, Dilani Gunawardhana, Ibrahim Misbah, Mohsen Rakhshandehroo, Steven H. Lin, Wei-Chuan Shih

Subject: Medicine And Pharmacology, Endocrinology And Metabolism Keywords: circulating tumor biomarkers; extracellular vesicles; biological nanoparticles; liquid biopsy; biosensing

Online: 31 January 2020 (05:18:15 CET)

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Working Paper ARTICLE

Circulating Endometrial Cells: A Tool in Endometriosis Diagnostics and Therapy Management

Vladimir Bobek, Eliska Pospisilova, Imrich Kiss, Helena Souckova, Pavel Tomes, Jan Spicka, Rafal Matkowski, Marcin Jedrzka, Katarina Kolostova, simone Ferrero

Subject: Medicine And Pharmacology, Obstetrics And Gynaecology Keywords: circulating endometrial cells; endometriosis; rare cells; menstrual cycle; liquid biopsy

Online: 18 August 2019 (16:36:07 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201811.0423.v1

The Circulating Transcriptome as a Source of Biomarkers for Melanoma

Carla Solé, Daniela Tramonti, Maike Schamm, Ibai Goicoechea, María Armesto, Luiza I. Hernandez, Lorea Manterola, Marta Fernandez-Mercado, Karmele Mujika, Anna Tuneu, Ane Jaka, Maitena Tellaetxe, Marc R. Friedländer, Xavier Estivill, Paolo Piazza, Pablo L. Ortiz-Romero, Mark R. Middleton, Charles H. lawrie

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: melanoma; plasma; liquid biopsy; miRNA; mRNA; biomarker; YRNA; RNA species

Online: 19 November 2018 (06:49:39 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.1184.v1

Histopathological Diagnosis of Primary Central Nervous System Lymphoma After Preoperative Corticosteroid Therapy

Julia Feldheim, Marvin Darkwah Oppong, Jonas Alexander Feldheim, Ramazan Jabbarli, Philipp Dammann, Anne-Kathrin Uerschels, Oliver Gembruch, Yahya Ahmadipour, Cornelius Deuschl, Andreas Junker, Ulrich Sure, Karsten Henning Wrede

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: primary central nervous system lymphoma; stereotactic biopsy; corticosteroid; histopathology; brain tumor

Online: 21 February 2024 (04:02:28 CET)

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Preprint REVIEW | doi:10.20944/preprints202308.2176.v1

Electrochemical Sensors for Liquid Biopsy and Their Integration into Lab-on-Chip Platforms: Revolutionizing the Approach to Diseases

Salma Umme, Giulia Siciliano, Elisabetta Primiceri, Antonio Turco, Iolena Tarantini, Francesco Ferrara, Maria Serena Chiriacò

Subject: Physical Sciences, Biophysics Keywords: Liquid biopsy; electrochemical sensors; Lab On Chip; miniaturization; sensors integration; microfabrication

Online: 31 August 2023 (10:40:48 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0406.v1

Single Droplet Microsensor for Ultra-Short Circulating EFGR Mutations Detection in Lung Cancer Based on Multiplex EFIRM Liquid Biopsy

Fang Wei, Peter Yu, Jordan Cheng, Feng Li, David Chia, David T.W. Wong

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Biosensors; ultra-short circulating tumor DNA; Lung Cancer; Liquid biopsy; EGFR

Online: 6 May 2023 (09:44:49 CEST)

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Working Paper ARTICLE

The Feasibility of Patient-Specific Circulating Tumor DNA Monitoring Throughout Multi-Modality Therapy for Locally Advanced Esophageal and Rectal Cancer: A Potential Biomarker for Early Detection of Subclinical Disease

Christopher Boniface, Christopher Deig, Carol Halsey, Taylor Kelley, Michael B. Heskett, Charles R. Thomas Jr., Paul T. Spellman, Nima Nabavizadeh

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: liquid biopsy; ctDNA; cell free DNA; non-operative management; neoadjuvant therapy

Online: 10 December 2020 (12:52:27 CET)

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Preprint ARTICLE | doi:10.20944/preprints201912.0357.v1

Microfluidic Device for On-Chip Immunophenotyping and Cytogenetic Analysis of Rare Biological Cells

Kumuditha M. Weerakoon-Ratnayake, Swarnagowri Vaidyanathan, Nicholas Larkey, Kavya Dathathreya, Mengjia Hu, Jilsha Jose, Shalee Mog, Keith August, Andrew K. Godwin, Mateusz L. Hupert, Malgorzata A. Witek, Steven A. Soper

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: microfluidics; immunophenotyping; FISH; liquid biopsy; circulating leukemia cells; circulating plasma cells

Online: 27 December 2019 (02:30:15 CET)

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Preprint REVIEW | doi:10.20944/preprints202309.0327.v1

Update on Myocarditis: From Etiology and Clinical Picture to Modern Diagnostics and Methods of Treatment

Ratko Lasica, Lazar Djukanovic, Lidija Savic, Gordana Krljanac, Marija Zdravkovic, Marko Ristic, Andjelka Lasica, Milika Asanin, Arsen Ristic

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: acute myocarditis; chronic inflammatory cardiomyopahty; heart failure; cardiac magnetic resonance; endomyocardial biopsy

Online: 6 September 2023 (02:42:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.1802.v1

Evaluating Stacked Methylation Markers for Blood-Based Multicancer Detection

Karen Funderburk, Sara R. Bang-Christensen, Brendan F. Miller, Hua Tan, Gennady Margolin, Hanna M. Petrykowska, Catherine Baugher, Nader Jameel, David Holland, Naomi S. Altman, Laura Elnitski

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Liquid biopsy; DNA methylation; biomarker; cancer; multi-cancer; bisulfite sequencing; diagnostics; screening

Online: 28 August 2023 (10:22:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202110.0124.v2

Triglyceride and Glucose Index as a Screening Tool for Nonalcoholic Liver Disease in Patients with Metabolic Syndrome

Anca Maria Amzolini, Fortofoiu Mircea-Catalin, Anca Barău Al-Hija, Ionela Mihaela Vladu, Diana Clenciu, Adina Mitrea, Maria Fortofoiu, Daniela Matei, Magdalena Diaconu, Marinela Sinziana Tudor, Elena Simona Micu

Subject: Medicine And Pharmacology, Gastroenterology And Hepatology Keywords: nonalcoholic liver disease; triglyceride and glucose index; metabolic syndrome; liver biopsy; obesity

Online: 8 October 2021 (13:34:38 CEST)

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Preprint REVIEW | doi:10.20944/preprints202108.0513.v1

Liquid Biopsies in Epilepsy: Biomarkers for Etiology, Diagnosis, Prognosis, and Therapeutics

Jordan H. Whitlock, Tabea M. Soelter, Avery S. Williams, Andrew A. Hardigan, Brittany N. Lasseigne

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: liquid biopsy; epilepsy; circulating biomarkers; cfDNA; lncRNA; tRNA; miRNA; diagnosis; prognosis; therapeutics

Online: 26 August 2021 (16:40:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0371.v1

Impact Of SARS-CoV-2 Pandemic On The Diagnosis Of Cervical Cancer And Precursor Lesions - A Single Center Retrospective Study

Lavinia Balan, Cristina Secosan, Virgiliu-Bogdan Sorop, Marilena Pirtea, ANCA MARIA CIMPEAN, Daniela Chiriac, Catalin Balan, Ema Borsi, Ariana Iorga, Laurentiu Pirtea

Subject: Medicine And Pharmacology, Obstetrics And Gynaecology Keywords: covid-19; sars-cov-2; cancer diagnosis; conization; cervical cancer; colposcopy; dysplasia; biopsy

Online: 4 April 2024 (10:59:38 CEST)

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Preprint REVIEW | doi:10.20944/preprints202403.1530.v1

Technical Advances in Circulating Cell-Free DNA Detection and Analysis for More Personalized Medicine in Patients’ Care

Monica Sorbini, Tullia Carradori, Gabriele Maria Togliatto, Tiziana Vaisitti, Silvia Deaglio

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: cell-free DNA; Next-generation Sequencing; digital PCR; liquid biopsy; non-invasive diagnostics

Online: 26 March 2024 (06:40:21 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.0693.v1

Phytotherapy Might Have a Role in Reducing Unnecessary Prostate Biopsies: Results From an Exploratory Randomized Controlled Trial of Two Different Phytotherapeutic Agents

Tommaso Cai, Irene Tamanini, Marco Puglisi, Leonardo Bizzotto, Michele Rizzo, Giovanni Liguori, Luca Gallelli, Alessandro Palmieri, Truls E Bjerklund Johansen

Subject: Medicine And Pharmacology, Urology And Nephrology Keywords: prostate; prostate specific antigen; prostate biopsy; curcuma; serenoa repens; boswellia; pinus and urtica.

Online: 10 November 2023 (11:40:44 CET)

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Preprint ARTICLE | doi:10.20944/preprints202306.0487.v1

Cell Free DNA Extracted From Csf for Molecular Diagnosis of Pediatric Embryonal Brain Tumors

Mathieu Chicard, Yasmine Iddir, Julien Masliah Planchon, Valérie Combaret, Valéry Attignon, Alexandra Saint-Charles, Didier Frappaz, Cécile Conter, Kevin Beccaria, Pascale Varlet, Birgit Geoerger, Sylvain Baulande, Gaelle Pierron, Yassine Bouchoucha, François Doz, Olivier Delattre, Joshua Waterfall, Franck Bourdeaut, Gudrun Schleiermacher

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Pediatric embryonal brain tumors, liquid biopsy, cell-free DNA, molecular diagnosis, nucleosome footprinting

Online: 7 June 2023 (05:48:23 CEST)

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Preprint REVIEW | doi:10.20944/preprints202304.1185.v1

Chasing Circulating Biomarkers in Renal Cell Carcinoma: the Case of Extracellular Vesicles and their miRNA Cargos

Ilenia Mastrolia, Virginia Catani, Marco Oltrecolli, Stefania Pipitone, Maria Giuseppa Vitale, Valentina Masciale, Chiara Chiavelli, Carlo Augusto Bortolotti, Cecilia Nasso, Giulia Grisendi, Roberto Sabbatini, Massimo Dominici

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: renal cell carcinoma; liquid biopsy; blood; extracellular vesicles; microRNA; biomarker; diagnostics; prognosis; prediction

Online: 28 April 2023 (13:00:31 CEST)

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Preprint REVIEW | doi:10.20944/preprints202212.0363.v1

Liquid Biopsy and Glioblastoma

Robert Eibl, Markus Schneemann

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Liquid biopsy; glioblastoma; GBM; cfDNA; ctDNA; brain tumor; MRD; monitoring; CTC; treatment response

Online: 20 December 2022 (09:00:47 CET)

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Preprint REVIEW | doi:10.20944/preprints202212.0300.v1

Medulloblastoma: From TP53 Mutations to Molecular Classification and Liquid Biopsy

Robert H. Eibl, Markus Schneemann

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: medulloblastoma; TP53 mutation; molecular classification; diagnostics; liquid biopsy; animal models; transcriptome; precision oncology

Online: 16 December 2022 (08:47:35 CET)

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Preprint ARTICLE | doi:10.20944/preprints202208.0208.v1

Plasma Cell-free RNA PD-L1 Expression and Overall Survival of Immune Checkpoint Inhibitor Therapy in Advanced Non-Small Cell Lung Cancer

Paul R. Walker, Mahvish Muzzafar, Sriraksha Jayananda, Praveen Namireddy, Nitika Sharma, Teresa Parent, Cynthia Cherry, Richard B. Lanman

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Plasma PD-L1; liquid biopsy; cfRNA; immune checkpoint inhibitor; predictive immune biomarker; NSCLC

Online: 11 August 2022 (06:10:34 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202111.0301.v1

A Model to Predict Upstaging to Invasive Carcinoma in Patients Preoperatively Diagnosed with Low-grade Ductal Carcinoma In Situ of the Breast

Luca Nicosia, Anna Carla Bozzini, Silvia Penco, Chiara Trentin, Maria Pizzamiglio, Matteo Lazzeroni, Germana Lissidini, Paolo Veronesi, Gabriel Farante, Samuele Frassoni, Vincenzo Bagnardi, Cristiana Fodor, Nicola Fusco, Elham Sajjadi, Enrico Cassano, Filippo Pesapane

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: ductal carcinoma in situ (DCIS); invasive breast carcinoma; breast; biopsy; overtreatment; active surveillance.

Online: 17 November 2021 (11:03:55 CET)

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Preprint ARTICLE | doi:10.20944/preprints202001.0351.v1

A Miniature Fibre-optic Raman Probe Fabricated by Ultrafast Laser Assisted Etching

Calum A. Ross, David G. MacLachlan, Brian Smith, Rainer J. Beck, Jonathan D. Shephard, Nick Weston, Robert R. Thomson

Subject: Physical Sciences, Optics And Photonics Keywords: Optical biopsy; Raman spectroscopy; Micro-optics; Ultrafast laser assisted etching; Femtosecond laser micromaching

Online: 29 January 2020 (10:38:18 CET)

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Preprint ARTICLE | doi:10.20944/preprints201905.0226.v1

Insights on CTC Biology and Clinical Impact Emerging from Advances in Capture Technology

Patrick Bailey, Stuart S. Martin

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Circulating Tumor Cells; CTC; Liquid Biopsy; CTM; CTMat; CTC Biology; CTC Capture Technology

Online: 17 May 2019 (16:15:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0937.v1

Incidence of Thyroid Cancer in Bethesda III Thyroid Nodules: A Retrospective Analysis at a Single Endocrine Surgery Center

Iyad Hassan, Lina-0 Hassan, Nahed Balalaa, Mohammad Askar, Hussa Alshehhi, Mohamed Almarzooqi

Subject: Medicine And Pharmacology, Surgery Keywords: FNAC, Bethesda classification, thyroid cancer; indeterminate nodules, thyroid nodules; ultrasound guided biopsy, thyroidectomy; 0

Online: 15 April 2024 (10:08:52 CEST)

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Background: Fine-needle aspiration cytology (FNAC) is widely used to diagnose and monitor thyroid nodules. The Bethesda System for Reporting Thyroid Cytopathology (TBSRTC) is the standard for interpreting FNAC specimens. The risk of malignancy in Bethesda III nodules also known as Atypia of Undetermined Significance (AUS) varies significantly throughout several studies published worldwide. This retrospective study examines the risk of cancer in thyroid FNAC categorized as Bethesda III, as identified in the final histopathology of thyroidectomy specimens at a single endocrine surgery center. Methods: This retrospective cohort analysis included 1038 consecutive patients who underwent elective thyroid surgery with complete follow up data between January 2020 and March 2024. Preoperative data on clinical and pathological characteristics have been collected. The final histopathology report from the thyroidectomy specimen was compared to the results of the preoperative FNAC on nodules that were judged to be Bethesda category III. Statistical methods were performed using SPSS version 29. Results: A total of 670 ultrasound-guided FNACs (64.5%) performed during the study period were included in the final analysis. The study population was predominantly female, represented by 79.6% of patients with a mean age of 42.5 (SD 12.1), while 20.4% were male and significantly older with mean age 45.13 years (p = 0.02). The FNAC inadequacy rate was 5.1%, which was associated with a high risk of malignancy (6 out of 34, 17.6%). Out of the total sample size of 170 patients classified as group III, 57 were found to have malignancies in final surgical histopathology, representing 33.5% of the cases within this category. The secondary gender-related outcome analysis showed that female patients classified under the Bethesda II category had a significantly higher risk of malignancy, with a rate of 21.2%, compared to males who had a malignancy rate of 3.4% in the same Bethesda category (p = 0.001, chi square test). However, female had more better prognostic non-invasive tumors than male (p = 0.02, chi square test). Conclusion: The study's results indicate that Bethesda categories II and III are associated with a higher risk of malignancy in comparison to the reports of the000 first and third editions of the TBSRTC, particularly for female patients classified under category II.

Preprint REVIEW | doi:10.20944/preprints202403.0086.v1

Advancements in Circulating Tumor Cell Research: Bridging Biology and Clinical Applications

Philip Salu, Katie M. Reindl

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Circulating tumor cells; liquid biopsy; cancer research; metastasis; personalized medicine; single-cell sequencing; biomarkers.

Online: 4 March 2024 (09:27:46 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.0681.v1

Cancer-associated Systemic Abnormality: A Novel Marker Panel for Early-stage Breast Cancer Characterization

Stefan Schreier, Prapaphan Budchart, Suparerk Borwornpinyo, Lakkana Adireklarpwong, Prakasit Chirappapha, Wannapong Triampo, Panuwat Lertsithichai

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: early-stage breast cancer; circulating tumor cells; rare cells, liquid biopsy; systemic cancer; cytomorphology

Online: 12 February 2024 (11:37:36 CET)

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Preprint ARTICLE | doi:10.20944/preprints202212.0485.v1

A Novel Modality Enables New Evidence-Based Individual Risk Stratification That Can Lead To Decisive Management and Treatment Decisions in Prostate Cancer

Meir Weksler, Avi Simon, Robert E. Lenkinski, Hagar Landsman, Haim Matzkin, Nicola Mabjeesh, Ilan Leibovitch

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: cell proliferation; epithelial glands; interactive biopsy guidance; malignancy score; risk stratification; individual treatment recommendations.

Online: 26 December 2022 (10:59:37 CET)

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Preprint COMMUNICATION | doi:10.20944/preprints202109.0133.v1

Fluid and Tissue Biomarkers of Lewy Body Dementia: Report of an LBDA Symposium

Gregory D. Scott, Moriah R. Arnold, Thomas G. Beach, Christopher H. Gibbons, Anumantha G. Kanthasamy, Russell M. Lebovitz, Afina W. Lemstra, Leslie M. Shaw, Charlotte E. Teunissen, Henrik Zetterberg, Angela S. Taylor, Todd C. Graham, Bradley F. Boeve, Stephen N. Gomperts, Neill R. Graff-Radford, Charbel E.-H. Moussa, Kathleen L. Poston, Liana S. Rosenthal, Marwan N. Sabbagh, Ryan R. Walsh, Miriam T. Weber, Melissa J. Armstrong, Jee A. Bang, Andrea C. Bozoki, Kimiko Domoto-Reilly, John E. Duda, Jori E. Fleisher, Douglas R. Galasko, James E. Galvin, Jennifer G. Goldman, Samantha K. Holden, Lawrence S. Honig, Daniel E. Huddleston, James B. Leverenz, Irene Litvan, Carol A. Manning, Karen S. Marder, Alexander Y. Pantelyat, Victoria S. Pelak, Douglas W. Scharre, Sharon J. Sha, Holly A. Shill, Mari Zoltan, Joseph F. Quinn, David J. Irwin

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: cerebrospinal fluid; alpha-synuclein; skin biopsy; seeded aggregation assays; tau, amyloid; Lewy body dementia

Online: 7 September 2021 (16:26:51 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202009.0736.v1

Cross-polarization Optical Coherence Tomography Probes for Intraoperative Application in Neurosurgery

Konstantin Yashin, Pavel Shilyagin, Elena Kiseleva, Grigory Gelikonov, Vladimir Romashov, Alexander Moiseev, Igor Medyanik, Sergey Ksenofontov, Ksenia Achkasova, Leonid Kravetz, Alexander Potapov, Natalia Gladkova

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: optical coherence tomography (CP OCT); cross-scattering; probes; brain tumors; stereotactic biopsy; neurosurgical guidance

Online: 30 September 2020 (10:23:55 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202210.0325.v2

Streptavidin-Modified Iron Oxide Hybrid Nanozymes for Magnetic Enrichment and Bioelectrocatalytic Sensing of Circulating Tumor RNA

Kevin M. Koo

Subject: Chemistry And Materials Science, Biomaterials Keywords: nanozymes; circulating tumor nucleci acid; liquid biopsy; magnetic enrichment; minimal residual disease; cell-free RNA

Online: 24 October 2022 (10:33:57 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201611.0105.v1

Comparison of Two EpCAM-Based Methods for CTC Detection and Molecular Characterization in Advanced Colorectal Cancer

Santiago Cabezas-Camarero, Virginia De La Orden, Silvia Veganzones-De-Castro, Beatriz Medievo-Valeros, Manuel Enrique Fuentes-Ferrer, Antonio Sánchez-Ruiz, Mariano Provencio, Enrique Aranda, Javier Sastre, Eduardo Díaz-Rubio

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: circulating tumor cells; CTC, liquid biopsy; KRAS; colorectal cancer; EpCAM; CellSearch; IsoFlux; castPCR; intratumor heterogeneity

Online: 21 November 2016 (09:52:20 CET)

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Preprint REVIEW | doi:10.20944/preprints202403.1312.v1

Will Liquid Biopsy Deliver the Expected Breakthrough in Clinical Oncology? Contribution of Mass Spectrometry-Based Proteomics

Pietro Traldi, Marco Agostini, Mahmoud Hamdan

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: Liquid biopsy; clinical oncology; mass spectrometry-based proteomics body fluids; circulating biomarkers; Protein post translational modifications

Online: 26 March 2024 (03:14:14 CET)

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Preprint ARTICLE | doi:10.20944/preprints202206.0420.v1

Role of Diffusion-weighted MRI in Differentiation between Benign and Malignant Anterior Mediastinal Masses

Tran Thi Mai Thuy, Nguyen Truong Hoang Trang, Tran Thanh Vy, Vo Tan Duc, Nguyen Hoang Nam, Phan Cong Chien, Le Huu Hanh Nhi, Le Huu Nhat Minh

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: anterior mediastinal lesions; diffusion weighted imaging; diffusion magnetic resonance imaging; gadolinium; thyroma; lymphoma; thymus neoplasms; biopsy

Online: 30 June 2022 (08:19:29 CEST)

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Background. To describe the characteristics of anterior mediastinal masses on conventional magnetic resonance imaging (MRI) and to assess the role of the Apparent Diffusion Coefficient (ADC) value in distinguishing benign from malignant mediastinal lesions. Methods. We conducted a retrospective cross-sectional study on 55 patients with anterior mediastinal mass who performed MRI before treatment. Biopsy and histopathological assessments were done after that. A radiologist evaluates the changes of signal intensity on these sequences: T1- weighted VIBE DIXON pre and post-contrast with Gadolinium, T2 HASTE, T2 TIRM, DWI/ADC, to determine the size, margin of the lesion, the presence of fat, cystic in it. ADCs values were calculated from the ADC maps which were constructed from b = 0 and b = 2000 Results. The study was composed of 55 patients, with 5 benign lesions and 50 malignant lesions. The ADCmean, ADCmedian, ADC10, and ADC90 in the histogram-based approach and hot-spot-ROI-based mean ADC for the malignant lesions was significantly lower than those found in benign lesions (P-value <0.05). The hot-spot-ROI-based mean ADC had the highest value in differentiation between benign and malignant mediastinal lesions, between group A (benign lesions, thymoma A, AB, B1) and group B (thymoma B2, B3 and other malignant lesions). The cut-off point of the ADC value differentiating malignant from benign mediastinal lesions was 1,17x10-3mm2/s with sensitivity of 80%, and specificity of 80%. The cut off point of the ADC value differentiating group A from group B was 0.99 x10-3mm2/s with sensitivity of 78,4%, and specificity of 88.9%. The cut-off point of the ADC value differentiating lymphoma from other malignant lesions was 0,91 x10-3mm2/s with sensitivity of 100%, and specificity of 60.5%. Conclusion. Diffusion-weighted MRI and measurement of ADC value in histogram-based approach and hot-spot-ROI-based mean ADC are very helpful in the differentiation between benign and malignant anterior mediastinal lesions.

Preprint ARTICLE | doi:10.20944/preprints202103.0330.v1

EndoScreen Chip: Serum Complement C9 Immunoassays Improve Risk Prediction and Early Diagnosis of Esophageal Adenocarcinoma

Julie A. Webster, Alain Wuethrich, Karthik B. Shanmugasundaram, Renee S. Richards, Wioleta M. Zelek, Alok K. Shah, Louisa Gordon, Bradley J. Kendall, Gunter Hartel, B. Paul Morgan, Matt Trau, Michelle M. Hill

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Barrett’s esophagus; biomarker; surveillance; screening; surface-enhanced Raman spectroscopy; SERS; complement component; liquid biopsy; lectin; glycoprotein.

Online: 12 March 2021 (08:03:55 CET)

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Preprint ARTICLE | doi:10.20944/preprints202310.1760.v1

Precise Detection and Transcriptomic Concordant Analysis of Circulating Tumor Cells from Prostate Cancer

Hyungseok Cho, Ki-Ho Han, Jae-Seung Chung, Seok-Soo Byun, Jae Il Chung, Won Ik Seo, Chan Ho Lee

Subject: Medicine And Pharmacology, Urology And Nephrology Keywords: prostate cancer; circulating tumor cells; lateral magnetophoresis; prostate-specific antigen; prostate-specific membrane antigen; biomarker; liquid biopsy

Online: 27 October 2023 (08:17:30 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202105.0524.v1

Exosomal Hsp70 in Liquid Biopsies - A Biomarker for Prediction and Response Monitoring in Cancer

Caroline Werner, Stefan Stangl, Lukas Salvermoser, Melissa Schwab, Maxim Shevstov, Alexia Xanthopoulos, Fei Wang, Daniel Medenwald, Martin Windberg, Matthias Bache, Martin Schlapschy, Arne Skerra, Gabriele Multhoff

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Hsp70; sandwich ELISA; liquid biopsy; tumor biomarker; exosomes; prediction; response monitoring; non-small cell lung carcinoma (NSCLC); glioblastoma

Online: 21 May 2021 (15:06:37 CEST)

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In contrast to normal cells, tumor cells of multiple entities overexpress the Heat Shock Protein 70 (Hsp70) not only in the cytosol, but also present it on their plasma membrane in a tumor-specific manner. Furthermore, membrane-Hsp70 positive tumor cells actively release Hsp70 into lipid microvesicles termed exosomes into the blood. Due to conformational changes of Hsp70 in the lipid environment, most commercially available antibodies fail to detect membrane-bound and exosomal Hsp70. To fill this gap and to assess the role of exosomal Hsp70 in the circulation as a potential tumor biomarker, we established the novel complete Hsp70 (compHsp70) sandwich ELISA using two monoclonal antibodies (mAbs) that are able to recognize both, free and lipid-associated Hsp70 on the cell surface of viable tumor cells and exosomes. The epitopes of the mAbs cmHsp70.1 (aa 451-461) and cmHsp70.2 (aa 614-623) that are conserved among different species reside in the substrate-binding domain of Hsp70, with measured affinities of 0.42 nM and 0.44 nM, respectively. Validation of the compHsp70 ELISA revealed a high intra- and inter-assay precision, linearity in a concentration range of 1.56 to 25 ng/ml, high recovery rates of ‘spiked’ liposomal Hsp70 (>84%), comparable values between human serum and plasma samples, and no interference by food intake or age of the donors. Hsp70 concentrations in the circulation of patients with glioblastoma, squamous cell or adeno non-small cell lung carcinoma (NSCLC) at diagnosis were significantly higher than those of healthy volunteers. Hsp70 concentrations dropped concomitantly with the decrease in viable tumor mass on irradiation of patients with approximately 20 Gy (range 18 – 22.5 Gy) or after completion of radiotherapy (60 - 70 Gy). In summary, the compHsp70 ELISA presented herein provides a highly sensitive and reliable tool for measuring free and exosomal Hsp70 in liquid biopsies of tumor patients, levels of which can be used as a predictive tumor-specific biomarker, risk assessment and for monitoring therapeutic outcome.

Preprint ARTICLE | doi:10.20944/preprints202102.0069.v1

Could Protein Content of Urinary Extracellular Vesicles be Useful to Detect Alcoholic Liver Disease and Assess Hepatocarcinoma Risk?

Gonzalez E., Azkargorta M., Garcia-Vallicrosa C., Prieto-Elordui J., Elortza F., Blanco-Sampascual S., Falcon-Perez JM

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: extracellular vesicles (EVs); urinary extracellular vesicles (uEVs); exosomes; biomarkers; liquid biopsy; cirrhosis; fibrosis; hepatocarcinoma (HCC); alcoholic liver disease (ALD).

Online: 1 February 2021 (18:15:22 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.1379.v5

Radiomics Machine Learning Analysis of Clear Cell Renal Cell Carcinoma for Tumour Grade Prediction Based on Intra-tumoural Subregion Heterogeneity

Abeer J. Alhussaini, J. Douglas Steele, Adel Jawli, Ghulam Nabi

Subject: Medicine And Pharmacology, Urology And Nephrology Keywords: clear cell renal cell carcinoma; ; renal masses; biopsy; computed tomography; radiomics; machine learning; tumour subregions; tumour heterogeneity; precision medicine

Online: 4 April 2024 (12:58:37 CEST)

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Background: Renal cancers are among the top ten causes of cancer-specific mortality, of which the ccRCC subtype is responsible for most cases. The grading of ccRCC is important in determining tumour aggressiveness and clinical management. Objectives: The objectives of this research were to predict the WHO/ISUP grade of ccRCC pre-operatively and characterise the heterogeneity of tumour sub-regions using radiomics and ML models, including comparison with pre-operative biopsy-determined grading in a sub-group. Methods: Data were obtained from multiple institutions across two countries, including 391 patients with pathologically proven ccRCC. For analysis, the data were separated into four cohorts. Cohorts 1 and 2 included data from the respective institutions from the two countries, cohort 3 was the combined data from both cohort 1 and 2, and cohort 4 was a subset of cohort 1, for which both the biopsy and subsequent histology from resection (partial or total nephrectomy) were available. 3D image segmentation was carried out to derive a voxel of interest (VOI) mask. Radiomics features were then extracted from the contrast-enhanced images, and the data were normalised. The Pearson correlation coefficient and the XGBoost model were used to reduce the dimensionality of the features. Thereafter, 11 ML algorithms were implemented for the purpose of predicting the ccRCC grade and characterising the heterogeneity of sub-regions in the tumours. Results: For cohort 1, the 50% tumour core and 25% tumour periphery exhibited the best performance, with an average AUC of 77.9% and 78.6%, respectively. The 50% tumour core presented the highest performance in cohorts 2 and 3, with average AUC values of 87.6% and 76.9%, respectively. With the 25% periphery, cohort 4 showed AUC values of 95.0% and 80.0% for grade prediction when using internal and external validation, respectively, while biopsy histology had an AUC of 31.0% for the classification with the final grade of resection histology as a reference standard. The CatBoost classifier was the best for each of the four cohorts with an average AUC of 80.0%, 86.5%, 77.0% and 90.3% for cohorts 1, 2, 3 and 4 respectively. Conclusion: Radiomics signatures combined with ML have the potential to predict the WHO/ISUP grade of ccRCC with superior performance, when compared to pre-operative biopsy. Moreover, tumour sub-regions contain useful information that should be analysed independently when determining the tumour grade. Therefore, it is possible to distinguish the grade of ccRCC pre-operatively to improve patient care and management.

Preprint ARTICLE | doi:10.20944/preprints202012.0806.v1

Active Surveillance in the Management of Ductal Carcinoma in Situ: The Radiologist’s Point of View

Luca Nicosia, Giuseppe di Giulio, Anna Carla Bozzini, Marianna Fanizza, Francesco Ballati, Anna Rotili, Matteo Lazzeroni, Antuono Latronico, Francesca Abbate, Giuseppe Renne, Francesca Addante, Marco Lucioni, Enrico Cassano, and Mauro Giuseppe Mastropasqua

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Ductal carcinoma in situ (DCIS); Invasive Breast Carcinoma; Underestimation; Upgrade Rate; Vacuum assisted breast biopsy (VABB); Breast Microcalcifications; Active Surveillance.

Online: 31 December 2020 (12:52:08 CET)

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Preprint ARTICLE | doi:10.20944/preprints202103.0777.v1

Prediction of Response to Atezolizumab Plus Nab-Paclitaxel in Unresectable Locally Advanced Triple Negative Breast Cancer (TNBC): the ClinicalUsefulness of PD-L1 mRNA Expression in Plasma-Derived Exosomes

Lucrezia Raimondi, Gian Paolo Spinelli, Paolo Ciracì, Filippo Maria Raimondi, Rachele Lazzeroni, Laura Di Benedetto, Stefano Valabrega, Laura Giaconi, Luigi Rossi, Giuseppe Naso

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Exosomal PD-L1 mRNA; extracellular vesicles; Triple Negative Breast Cancer; Immunotherapy; PD-L1 axis; Atezolizumab – nab-paclitaxel; Predictive biomarkers; Liquid biopsy

Online: 31 March 2021 (15:27:45 CEST)

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Preprint REVIEW | doi:10.20944/preprints201908.0186.v1

Roles of Extracellular Vesicles (EVs) Carrying HSPs in Cancer Biomarkers, Immune Surveillance, and Immune Evasion

Eman Taha, Kisho Ono, Takanori Eguchi

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: heat shock protein (HSP); extracellular vesicle (EV); exosome; oncosome; immune evasion; resistance-associated secretory phenotype (RASP); EMT; hypoxia; biomarker; liquid biopsy

Online: 17 August 2019 (16:15:01 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202201.0390.v1

Analytic and clinical validation of a pan-cancer NGS liquid biopsy test for the detection of copy number amplifications, fusions and exon skipping variants.

Audrey Audetat, Cherie Tschida, Sarah Kreston, Adam Stephen, Brittany D'Alessio, Brittany D’Alessio, Leisa Jackson, John Williamson, Hestia Mellert, Niki Givens, Ubaradka G. Sathyanarayana, Gary A. Pestano

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: non-small cell lung cancer (NSCLC); liquid biopsy; cell-free nucleic acid (cfNA); next generation sequencing (NGS); CNA (copy number amplifications); fusions, exon-skipping.

Online: 26 January 2022 (03:21:12 CET)

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Preprint ARTICLE | doi:10.20944/preprints202201.0380.v1

Circulating Tumor Cell Assay to Non-Invasively Evaluate PD-L1 and Other Therapeutic Targets in Multiple Cancers

Raymond Page, Darshana Patil, Dadasaheb Akolkar, Sudha S Murthy, Kiran Bendale, Revati Patil, Pradeep Fulmali, Pooja Fulmali, Archana Adhav, Sneha Puranik, Sachin Apurwa, Vineet Datta, Chirantan Bose, Stefan Schuster, Jinumary John, Ajay Srinivasan, Rajan Datar

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Therapeutic biomarkers; circulating tumor cells; Immunocytochemistry; targeted therapy; Fluorescence in situ hybridization (FISH); Immune Checkpoint Inhibitors; Liquid Biopsy; Hormonal Treatments; Breast Cancer; Lung Cancer

Online: 25 January 2022 (11:31:05 CET)

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Preprint ARTICLE | doi:10.20944/preprints202301.0280.v1

Early Diagnosis of Oral Cancer and Lesions in Fanconi Anemia Patients: A Prospective and Longitudinal Study Using Saliva and Plasma

Ricardo Errazquin, Estela Carrasco, Sonia Del Marro, Anna Suñol, Jorge Peral, Jessica Ortiz, Juan C. Rubio, Carmen Segrelles, Marta Dueñas, Alicia Garrido-Aranda, Martina Álvarez, Cristina Belendez, Judith Balmaña, Ramon Garcia-Escudero

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Fanconi anemia; oral cancer; oral potentially malignant lesion; liquid biopsy; saliva; oral rinse; plasma; next generation sequencing; cancer gene panel; early diagnosis; diagnostic test; deep sequencing

Online: 16 January 2023 (09:10:06 CET)

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Preprint REVIEW | doi:10.20944/preprints202201.0366.v1

Minimal Residual Disease Using Liquid Biopsies in Hematological Malignancies

Rafael Colmenares, Noemí Álvarez, Santiago Barrio, Joaquín Martínez-López, Rosa Ayala

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: cell-free DNA; liquid biopsy; cancer; next-generation sequencing (NGS); minimal residual disease; measurable residual disease; molecular residual disease (MRD); leukemia; lymphoma; myeloma; myeloproliferative neoplasms; myelodysplastic syndrome

Online: 25 January 2022 (08:13:24 CET)

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Preprint ARTICLE | doi:10.20944/preprints202108.0210.v1

Kidney Pathology and Outcomes in Anca-Associated Vasculitis: Retrospective Analysis of 85 Patients

Elena Zakharova, Anastasiia Zykova, Tatyana Makarova, Eugenia Leonova, Ekaterina Stolyarevich

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: microscopic polyangiitis; granulomatosis with poliangiitis; eosinophilic granulomatosis with poliangiitis; kidney biopsy; pauci-immune focal and segmental necrotizing and crescentic glomerulonephritis; tubular atrophy and interstitial fibrosis; kidney survival

Online: 10 August 2021 (07:59:00 CEST)

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