The mechanism of sex determination and differentiation in animals remains a central focus of reproductive and developmental biology research, and the regulation of sex differentiation in amphioxus remains poorly understood. Cytochrome P450 Family 19 Subfamily A member 1 (CYP19A1) is a crucial sex differentiation gene that catalyzes the conversion of androgens into estrogens. In this study, we identified two aromatase-like genes in amphioxus: cyp19-like1 and cyp19-like2. Cyp19-like1 is more primitive and may represent the ancestral form of cyp19 in zebrafish and other vertebrates. Cyp19-like2, on the other hand, is likely the result of gene duplication within am-phioxus suggesting its potential role as a key gene in sex differentiation. To gain further insights into the expression patterns of these two aromatase-like, we examined their expression in different gonad tissues and during different stages of gonad development. While the expression patterns of the two genes differ in gonad tissues, both are highly expressed in the gonad primordium and are primarily localized to microsomal membrane systems. However, as development proceeds, their expression levels decrease significantly. This study enhances our understanding of sex dif-ferentiation mechanisms in amphioxus and provides valuable insights into the formation and evolution of sex determination mechanisms in vertebrates.

More than 20 years after the discovery of the first anelloviruses (AVs), they still remain a mysterious group of viruses. To date, there is no clear understanding of their impact on the host organism, although information is emerging about their participation in various pathologies. However, it is well known that AVs are an important component of the human virome. In the current study we assessed the abundance of AVs in viromes from four nasal and pharyngeal samples from children with respiratory symptoms using a metagenomic approach. It is worth noting that three patients had known respiratory viruses and one did not have any. Regarding AVs, they were detected in all samples, namely Torque teno mini virus (TTMV). The greatest abundance and diversity of AVs (Torque teno virus (TTV), TTMV and Torque teno midi virus (TTMDV)) were shown in a diseased patient with an absence of respiratory viruses, which may indicate the potential involvement of AVs in the maintenance of respiratory symptoms. An activated immune system to combat the main pathogens, against the background of which the number of AVs decreases, is discussed as a possible reason for the reduced diversity of anellome in other patients. We also compared the results of the metagenomic approach and qPCR for major respiratory viruses, which were in agreement. In addition, metagenomic sequencing made it possible to obtain additional data on viral genomes in order to establish their taxonomic identification and characterize individual viral profiles in patients from the Siberian part of Russia.

Biodiversity has become an issue of global anxiety over the past decades due to its rapid decline worldwide. Bangladesh as one of the most densely populated countries in the world is no more exception. The country, although, was once very rich in biodiversity, during the last few decades as a consequence of the rapid reduction in forest area, urbanisation, habitat modification, unsustainable natural resources use and collection and overall climate change it has decreased alarmingly. Of late, the government, as a signatory of various regional and international conservation treaties, has taken various initiatives to improve country’s dwindling biodiversity. This paper reviews the present situation of biodiversity in Bangladesh, management trends and major causes of biodiversity loss. A separate statutory body is fundamental to ensure conservation, sustainable use and equitable sharing of benefits arisen from biodiversity in the country.

We generalize the point information gain (PIG) and derived quantities, i.e., point information gain entropy (PIE) and point information gain entropy density (PIED), for the case of the Rényi entropy and simulate the behavior of PIG for typical distributions. We also use these methods for the analysis of multidimensional datasets. We demonstrate the main properties of PIE/PIED spectra for the real data on the example of several images, and discuss further possible utilizations in other fields of data processing.

The protection and regulation of international trade of Australia’s native species is legislated by its Environment Protection and Biodiversity Conservation Act of 1999. In response to overwhelming evidence that the Act is ineffective at protecting biodiversity, the Australian Government is currently reforming its environmental laws. One problematic component of the Act is in cases where threatened species interact with multiple regulatory and management frameworks, such as commercially fished marine species that are traded as seafood. Here, we analyzed international seafood trade data and found that Australia exports four threatened fish species listed under the Act and additional species acknowledged as globally threatened. In light of our analysis, we have three recommendations for Australia’s new environmental legislation: 1) apply the same precautions to commercially harvested species as to other threatened species; 2) review the status of all threatened species annually; and 3) assess species listed on global conservation conventions. These recommendations will help ensure that Australia’s marine biodiversity is protected, despite its commercial value.

Mangrove forests provide a wide range of goods and services that sustain communities around the world. This paper explores utilization patterns of extractable mangrove resources by communities within the proposed Kenya – Tanzania Transboundary Conservation Area (TBCA). Some 152 household surveys and 12 Nominal Group Technique discussions were carried out. At least 16 direct use products were reported to be extracted from the mangroves, with 90% of households found to use mangrove products. Changing patterns of mangrove use in the areas and accessibility point towards unsustainable utilization and promote illegal activities. Overharvesting of mangrove wood products was reported to be the major threat facing the forests. Patterns of mangrove use in the study site reveal a high level of mangrove-dependence by communities for their livelihood. There is a need to address the problems of open access through capacity building and awareness creation, development and implementation of harvest plans as well as introduction of alternative livelihood options to the communities in the area.

Biodiversity plays an important role in maintaining the ecological balance of the earth. The study of traditional knowledge related to biological resources is a hot issue in the field of international biodiversity conservation. Xishuangbanna is a key area of biodiversity and a cultural hotspot of international significance. According to the Technical Regulation for Classification, Investigation, and Inventory of Traditional Knowledge Relating to Biological Diversity issued by the Ministry of Ecology and Environment, we investigated and catalogued the traditional knowledge related to biodiversity of the Jino people who have lived in Xishuangbanna for generations, and collected 490 entries of traditional knowledge related to biodiversity of the Jino people. Drawing on the traditional knowledge diversity index calculation method proposed by Wang Guoping, the overall traditional knowledge α-diversity index of the Jino people is 0.63, indicating that the richness of the traditional knowledge of the Jinuo people is relatively high. The traditional culture related to biodiversity, the traditional knowledge related to agricultural genetic resources, and the traditional technology related to the sustainable utilization of biological resources are relatively rich and diverse. The diversity index is 0.86, 0.82 and 0.79 respectively. In addition, Xishuangbanna has invested a lot of energy in biodiversity protection, including the establishment of nature reserves, botanical gardens, zoos, ecological tea gardens and other species reserves, the promulgation of laws and policies related to biodiversity protection, and has achieved remarkable results in in-situ protection and ex-situ protection. On the basis of analyzing the progress and achievements of biodiversity conservation in Xishuangbanna, this study points out that Xishuangbanna faces challenges such as the loss of traditional knowledge, insufficient conservation efforts, and great changes in land use, and puts forward corresponding suggestions.

The preservation of biodiversity has become a major concern in contemporary society. It is essential to understand teachers' representations of biodiversity to effectively guide educational efforts because of the significant role of education in raising awareness and promoting sustainable behavior. Through a survey of a sample of 118 teachers, we adopted a mixed-method approach combining both a closed questionnaire and interviews. We used these methods to explore teachers' representations of the concept of biodiversity as an integral part of the school curriculum, as well as to gather their views on certain features of its teaching. The results state that the majority of teachers perceive biodiversity as a whole and in its most complex relationships. During their professional experience, they have developed a biocentric and anthropocentric conception of biodiversity. This study provides valuable information to guide future pedagogical practices and educational policies in sustainable education.

The term “conservation,” as it relates to biodiversity in a Western context, has had a contested history and as conservation science and societal values have evolved, consensus over its precise meaning has remained elusive. The broad scope of contemporary definitions hampers effective communication during a period of environmental crisis and is troublesome for any derivative concept which aims to quantify the efforts of the conservation community. This presents an avoidable hindrance to the systematic planning of the conservation field. To remedy this situation, we provide an outcome separation framework that is based on the expected degree of separation of the action’s proximate outcome from its intended, ultimate outcome for native habitat and/or native or ecological replacement species. Framing a definition of conservation through this lens of outcome separation allows for conservation-related actions to be clearly categorized into one of three discrete tiers (primary, secondary, and tertiary) based on both the proximate outcome’s degree of separation from its intended, ultimate outcome and the conservation status of native habitat and/or native or ecological replacement species. A distillation of this tiered framework also provides a fully inclusive, succinct definition of biodiversity conservation that is resilient to future conceptual evolutions of the field.

Germplasm is a valuable natural resource in plant diversity that is crucial for its potential use. It provides knowledge about a species genetic composition. Germplasm protection strategies are not just planting hope threatened with extinction, they preserve medicinal and other essential plants on which survival rests. The successful use of genetic plant resources necessitates diligent collection, storage, analysis, documentation, and germplasm exchange. Slow growth cultures, cryopreservation, pollen and DNA banks, botanic gardens, genetic reserves and farmer’s fields are few conservation techniques. However, usage of an in vitro procedure with any chance of genetic instability leads to the destruction of the entire substance. Improved understanding of basic regeneration biology would, in turn, undoubtedly increase the capacity to regenerate plants from in vitro harvested explants, thus expanding selection possibilities. Germplasm conservation seeks to conserve endangered and vulnerable plant species worldwide for future proliferation and development; it is also the bedrock of agricultural production.

We evaluated progress towards animal biodiversity research in Georgia, a key area in the Caucasus biodiversity hotspot. By reviewing recently (1990-2018) published articles in all areas of animal diversity research, we unmasked the trends in biodiversity inventory, ecological and biogeographical studies, and conservation issues in Georgia. We concluded that species inventory and biodiversity research in Georgia has significantly increased during the last ten years, however the rate and extent of investigation is far from satisfactory. Major gaps remain in all branches of animal diversity research in Georgia, and consequently existing knowledge is inadequate to address modern challenges related to species and ecosystem conservation. We urge local governmental authorities and international scientific societies to support development of stronger research facilities and cultivate interest in biodiversity inventory and research in Georgia as an important step towards maintaining globally important biodiversity in the Caucasus.

Abstract: Dispersal strategies of a species can affect its invasion success. Investigation into the dispersal strategies of invasive species in relation to different factors helps our understanding of the invasion mechanisms and provides knowledge for population management and invasion evaluation. Tetranychus ludeni Zacher (Acari: Tetranychidae) is an invasive species, which is native to Europe but now cosmopolitan. Here, we examined the effects of age and density on dispersal in mated females. Our results show that older females with more eggs were more likely to disperse and moved longer distances than younger ones with fewer eggs. Older females spread most of their eggs out of the natal habitats and over longer distances, which reduced competition and increased offspring fitness. Females significantly increased dispersal probability and distance with the increase of population density to avoid crowding. The synchronization of dispersal and reproduction, along with the positive density-dependent dispersal strategy, may facilitate habitat colonization and invasion speed of T. ludeni.

DNA barcoding has emerged as a powerful tool in the field of taxonomy, transforming the way we identify and classify species. This review article aims to provide an up-to-date overview of the advancements in DNA barcoding techniques, their applications, and their impact on taxonomy and biodiversity studies. We will explore the evolution of DNA barcoding, the development of standardized barcode regions, and the challenges and limitations associated with this methodology. Additionally, we will delve into the diverse range of taxonomic groups for which DNA barcoding has been applied, highlighting key case studies and their contributions to our understanding of biodiversity. Furthermore, we will discuss the integration of DNA barcoding with other molecular and bioinformatic approaches, such as metabarcoding and machine learning, to enhance species identification and biodiversity assessment. This review aims to provide a comprehensive perspective on how DNA barcoding is reshaping the field of taxonomy and its implications for conservation, ecology, and evolutionary biology.

Abandoned, lost, or otherwise discarded fishing gear (ALDFG) is a primary contributor to the entanglement of numerous marine species. Utilizing digital media platforms like Google, Facebook, and Instagram we conducted an assessment of the detrimental effects of ALDFG and plastic litter, on the biodiversity of Italy's marine ecosystem. Our investigation revealed that plastic litter has adverse consequences on various forms of marine life, including reptiles, mammals, birds, fish, crustaceans, and mollusks, along different Italian geographical sub-areas. Several records of interaction between plastic and endangered and vulnerable marine species have been described. Our reports even highlight the impact on marine turtles, mainly on Caretta caretta. This is the first report of entangled with fishing nets in Cetorhinus maximus, Oblada melanura, Serranus scriba, Homarus gammarus, Octopus vulgaris, and Phoenicopterus roseus. Furthermore, we identified that ghost nets have repercussions for endangered and vulnerable marine species. This update sheds light on the ongoing adverse effects of ghost nets on the Italian marine ecosystem, yet it is crucial to acknowledge that the true extent of the problem remains underestimated.

This article delves into the pivotal role that biodiversity of soil microbes plays in bolstering tree health and overall agroecosystem productivity. With the advent of regenerative agricultural practices, there has been a resurgence of interest in understanding the complex interactions between soil microbiota and plant roots, particularly through processes like rhizophagy. Rhizophagy, the digestion of living root cells by soil microorganisms, stands out as a fundamental mechanism in nutrient cycling and overall tree vitality. By examining the impact of enhanced microbial diversity on tree health, we shed light on the practical applications, such as the utilization of the BEAM Inoculant product, which has demonstrated remarkable efficacy in augmenting soil health and, consequently, tree Vigor

Despite several decades’ effort to detect and identify phytoplasmas (Tenericutes: Mollicutes) using PCR and Sanger sequencing focusing on diseased plants, knowledge of phytoplasma biodiversity and vector associations remains highly incomplete. To improve protocols for documenting phyto-plasma diversity and ecology, we used DNA extracted from phloem-feeding insects and com-pared traditional Sanger sequencing with a next-generation sequencing method, Anchored Hy-brid Enrichment (AHE) for detecting and characterizing phytoplasmas. Among 22 of 180 leaf-hopper samples that initially tested positive for phytoplasmas using qPCR, AHE yielded phyto-plasma 16Sr sequences for 20 (19 complete and 1 partial sequence) while Sanger sequencing yield-ed sequences for 16 (11 complete and 5 partial). AHE yielded phytoplasma sequences for an addi-tional 7 samples (3 complete and 4 partial) that did not meet the qPCR threshold for phytoplasma positivity or yielded non-phytoplasma sequences using Sanger sequencing. This suggests that AHE is more efficient for obtaining phytoplasma sequences. Twenty-three samples with sufficient data were classified into eight 16Sr subgroups (16SrI-B, I-F, I-AO, III-U, V-C, IX-J, XI-C, XXXVII-A), three new subgroups (designated as 16SrVI-L, XV-D, XI-G) and three possible new groups. Our results suggest that screening phloem-feeding insects using qPCR and AHE sequencing may be the most efficient method for discovering new phytoplasmas.

Many tropical invasive species have allelopathic effects that contribute to their success in native plant communities. Pyrolyzed biomass (“biochar”) can sorb toxic compounds, including allelochemicals produced by invasive plants, potentially reducing their inhibitory effects on native species. Strawberry guava (Psidium cattleianum) is among the most important allelopathic invasive species in tropical islands and recognized as the most serious invasive species threat in the global biodiversity hotspot of Mauritius. We investigated the effects of additions of locally produced biochar on native tree species in a field experiment conducted in areas invaded by strawberry guava within Mauritius’ largest national park. Growth and survivorship of native tree species were monitored over 2 ½ years in plots subjected to four treatments: non-weeded, weeded, weeded + 25 t/ha biochar and weeded + 50 t/ha biochar. Native tree growth and survivorship were strongly suppressed by strawberry guava. Biochar treatments dramatically increased native tree performance, with more than a doubling in growth, and substantially increased native tree survivorship and species diversity, while suppressing strawberry guava regeneration, consistent with growth-promoting properties and sorption of allelochemicals. We conclude that biochars, including “sustainable biochars” produced from locally accessible biomass using low-tech pyrolysis systems, have considerable potential to counteract effects of allelopathic invaders and increase the capacity for native species regeneration in tropical island ecosystems.

Governance is one of the most essential instruments for environmental management. Biodiversity is in the core field of environmental governance. Yet environmental authorities are persistently challenged the loss of biodiversity as a very important global issue for several years due to high dependent exposure to risks. The study attempts to relook at the key governance tools that strengthen policies towards managing biodiversity within and around the national park’s survey in Moulvibazar district. The study showed that biodiversity related legislation amended was the highest in Bangladesh for the period of 2010 to 2016. The growth of policy instruments maximized at but in low environmental governance services within the same period. The study assessed that the existing environmental policy instrument is inadequate and sluggish for effective conservation, compared with several others governance tools and various performances are still below par. Governance knowledge is indispensable for biodiversity management but such knowledge is poorly identified. These results reflect the importance of effective governance for transparency that the State provides. The research is to represent a dynamic and adaptable framework that can be applied for collective governance relevant to policy integration, participation and enforcement in order to foster environmental conservation sustainability.

Despite of being an exceptionally biodiversity rich country, the forest coverage of Bangladesh is declining at an alarming rate. Declaration and management of protected areas in this regard is one of the efforts from government side to tackle the loss of biodiversity. The limited numbers of forest-protected areas (FPA), established to conserve the dwindling forest biodiversity of the country with high pressure on them for timber, non-timber forest products, and fuelwood - makes their management challenging. Moreover, most of the FPAs of the country declared only in the recent decades with very limited infrastructure, manpower and policy support for monitoring and governance. Some people-centred approaches for the management of FPAs and alternative livelihood and income generation subsidies although made available through a few project interventions, their number are still inadequate and performance remains less than satisfactory. This chapter provides a critical review of the FPAs of Bangladesh looking at their role in biodiversity conservation, management challenges, and key lessons from previous management interventions with recommendations for the future. It has been revealed that the FPA system of Bangladesh still poorly represents the diverse forest ecosystems with relatively small forest size and lack of corridors for the movement of wildlife. There are ample opportunities to render co-management of FPAs an effective strategy to minimize the conflicts in FPAs management in the country. It is, however, important to ensure the access of local forest-dependent people to different alternative income generating options that may adequately support their livelihoods.

Ambitious biodiversity goals to protect 30% or more of the Earth’s surface by 2030 (30x30) require strategic near-term targets. To define areas that must be protected to prevent the most likely and imminent extinctions, we propose Conservation Imperatives—16,825 unprotected sites harboring rare and threatened species and spanning ~164 Mha of the terrestrial realm. We estimate that protecting the Conservation Imperatives would cost approximately US$169 billion [90% probability: $146 — $228 billion]. Globally, 38% of the 16,825 sites are either adjacent to or within 2.5 km of an existing protected area, potentially reducing land acquisition and management costs. These sites should be prioritized for conservation action over the next five years as part of a broader strategy to expand the global protected area network. The expansion of global protected areas between 2018–2023 incorporated only 7% of sites harboring range-limited and threatened species, highlighting a renewed urgency to conserve these habitats. Permanently protecting only 0.74% of land found in the tropics, where Conservation Imperatives are concentrated, could prevent the majority of predicted near-term extinctions once adequately resourced. We estimate this cost to be US$29 billion to US$46 billion per year over the next five years. Multiple approaches will be required to meet long-term protection goals: providing rights and title to Indigenous Peoples and Local Communities (IPLCs) conserving traditional lands, government designation of new protected areas on federal and state lands, and land purchase or long-term leasing of privately held lands.

The possibility of abrupt transitions threatens to poise ecosystems into irreversibly degraded states. Recently, it has been proposed the use of engineered microbiomes in endangered ecosystems to prevent them to cross tipping points and avoid collapse. Potential targets for such interventions include some of the most prominent life-support systems in the biosphere: drylands and coral reefs. Since engineering can require the introduction of microorganisms not present in resident communities, how can we weight the potential outcomes? One way is to use general models of species interactions where the "synthetic" strain is incorporated into a standard multispecies model. Here we follow this approach by modelling a resource-consumer community where one of the species is a modified one that acts by preserving some key resource. We show how the indirect effect of damping the decay of shared resources results in biodiversity increase, and last but not less, the successful incorporation of the synthetic within the ecological network. Further extensions and implications for future restoration and terraformation strategies are discussed.

Due to a lack of knowledge about arthropod biodiversity in Egyptian organic agro-ecosystems; the study aimed to introduce information on the diversity, richness, and distribution of insect and mite species in two organic agro-ecosystems, also, to investigate the impact of plant-arthropod interactions. Samples collected from two organic farms, i) Shampoliah farm, Fayoum (GCS 29°21'07.4"N 30°44'17.8"E), and ii) SEKEM farm, Sharkia (GCS 18 30°22'56.1"N 31°39'17.4"E). Results shown 39 species recorded in Shampoliah farm, and 35 species in SEKEM of mite, insect, medicinal, and weed species. When 14 species shared among two sites. Study has measured the H', D and 1/D indices within each location, and the similarity/dissimilarity between locations. The study hypothesized the possible plant-arthropod interactions that explain why diversity differs from an ecosystem to another; due to; plant size, plant morphological characters, soil fertilization, plant nutritional content, and the prey-predator interactions. The added hypothesis; is to show that the importance of natural habitat is supporting natural enemies and distribution of arthropods, which could vary dramatically with the type of pest species, IPM, and landscape type considered.

Sago (Metroxylon spp.) is an important crop in Maluku. This study aims to identify the biodiversity of sago palm and understorey vegetation around sago clumps in Maluku. The research was carried out in six sago area from September 2015 to October 2016. The Sago Plant identification was carried out through the growth phase of sago, i.e. seedlings, saplings, weaning, trunks, and ripening. Vegetation observation was done in radius 100 m2 surrounding sago clumps. The result shows that Metroxylon rumphii Mart type. (Tuni sago), M. sagus Rottb. (Molat sago) and M. Silvester Mart. (Ihur Sago) dominates sago palms area in Seram and Ambon Islands, Maluku. There are significant morphological differences between the types of sago, especially in stem height, midrib width, leaf midrib colour, number of thorns, and flower stalk length, as well as the difference of carbohydrate content. Understorey vegetation of each observation sites diverse consist of 15 families and 20 species. The families that dominate the vegetation under the sago palms are Araceae, Thelypteridaceae, and Athyriaceae. The types of plants from Araceae are taro types and broadleaf, while those from the Thelypteridaceae and Athyriaceae families are types of ferns.

The destruction of natural habitat for cropland and pastures represents a major threat to global biodiversity. Despite widespread societal concern about biodiversity loss associated with food production, consumers access to quantitative estimates of the impact of crop production on the world’s species has been very limited compared to assessments of other environmental variables such as greenhouse gas emissions or water use. Here, we present a consistent dataset of the biodiversity footprints of pastures and 175 individual crops at country level. The data were generated by combining maps of the global distribution of agricultural areas in the year 2000 with spatially explicit estimates of the biodiversity loss associated with the conversion of natural habitat to farmland. Estimates were derived for three common alternative measures of biodiversity – species richness, threatened species richness, and range rarity – of the world’s mammals, birds, and amphibians. Our dataset provides important quantitative information for food consumers and policy makers, allowing them to take evidence-based decisions to reduce the biodiversity footprint of global food production.

Leclère et al.¹ have outlined the possibility of a biodiversity transition for the 21^st century, a line of thinking equivalent to the Forest Transition theory and what it says about forest cover globally². The authors use a suite of global models to explore the impacts on global biodiversity of interventions on land-use, consumption and production patterns. They outline six strategies that have the potential to stop the downfall of global terrestrial biodiversity by 2050 and redress it to a pre-1970 level by 2100. Although robust, sophisticated and well-illustrated, the conclusions of this paper cannot alone be used to frame a post-2020 biodiversity strategy.

Beer is a fermented beverage with a history as old as human civilization and its productive process has been spread all around the world becoming unique in every country and iconic of entire populations. Ales and lagers are by far the most common beers; however, the combination of raw materials, manufacture techniques and aroma profiles are almost infinite, so it is not surprising to notice that there is a large amount of different beer styles, each of them with unique characteristics. Nowadays, diversification is becoming increasingly important in the brewing market and the brewers are continuously interested in improving and extending the already wide range of products, especially in craft brewery. One of the major components that can have a deep impact on the final product is yeast, since it is able to convert carbohydrates in wort, especially maltose and maltotriose, into ethanol, carbon dioxide and other minor aroma-active compounds. Saccharomyces cerevisiae (top‐fermenting yeasts used to produce ales) and Saccharomyces pastorianus (cryotolerant bottom‐fermenting hybrids between S. cerevisiae and Saccharomyces eubayanus responsible for the fermentation of lagers) are most used in breweries. However, an increasing number of different yeast starter cultures are commercially available, to improve the production efficiency also at relative low temperatures and to obtain desirable and diversified aroma profiles avoiding undesired compounds. Four main genetic engineering-free trends are becoming popular in craft brewing yeast development: 1) the research for novel reservoirs as source of new performant S. cerevisiae yeasts; 2) the creation of synthetic hybrids between S. cerevisiae and Saccharomyces non-cerevisiae in order to mimic lager yeasts by expanding their genetic background; 3) the exploitation of evolutionary engineering approaches; 4) the usage of non-Saccharomyces yeasts either in co-coculture or in sequential fermentation with S. cerevisiae. In the present work we summarized pro and contra of these approaches and provided an overview on the most recent advances on how brewing yeast genome evolved and domestication took place. Finally, we delineated how the correlations maps between genotypes and relevant brewing phenotypes can assist and further improve the search for novel craft beer starter yeasts.

Hyperspectral images are an important tool to assess ecosystem biodiversity both on terrestrial and benthic habitats. To obtain more precise analysis of biodiversity indicators that agree with indicators obtained using field data, analysis of spectral diversity calculated from images have to be validated with field based diversity estimates. The plant species richness is one of the most important indicators of biodiversity. This indicator can be measured in hyperspectral images considering the Spectral Variation Hypothesis (SVH) which states that the spectral heterogeneity is related to spatial heterogeneity and thus to species richness. The goal of this research is to capture spectral heterogeneity from hyperspectral images for a terrestrial neo tropical forest site using Vector Quantization (VQ) method and then use the result for prediction of plant species richness. The results are compared with that of Hierarchical Agglomerative Clustering (HAC). The validation of the process index is done calculating the Pearson correlation coefficient between the Shannon entropy from actual field data and the Shannon entropy computed in the images. Terrestrial dry forest and marine coastal hyperspectral images with different resolutions have been used for spectral diversity feature validation.

The food system, the most important driver of planetary transformation, is in a deep crisis. Therefore, seeking a sustainable and socially-fair transition pathway becomes an issue of utmost priority for our own survival. The consideration of food as a commodity, a social construct that played a central role in driving this crisis, remains the uncontested narrative to lead the different transition pathways what seems rather contradictory. By exploring the normative values in the transition landscape, this paper seeks to understand how relevant is the hegemonic narrative of “food as commodity” and its alternative of “food as commons” to determine transition trajectories and food policy beliefs. Applying the Multi-level Perspective framework and developing the ill-studied “agency in transition”, this research enquired food-related professionals that belong to an online community of practice (N=95) on valuation of food dimensions and agency in food transitions to check whether the valuation of food is relevant to explain personal stances in transition. Results suggest the socially-constructed view of food as commodity is positively correlated to the gradual reforming attitude, whereas food as commons is positively correlated to the counter-hegemonic transformers regardless the self-defined position in the transition landscape (regime or niches). At personal level, there are multiple loci of resistance with counter-hegemonic attitudes in varied institutions of the regime and the innovative niches, many of them holding this discourse of food as commons. Conversely, alter-hegemonic attitudes are not positively correlated to this alternative discourse and they may inadvertently or purportedly reinforce the ‘‘neoliberal narrative’’. Food as commons, a different narrative whose rationale is explained in the paper, seems to be a relevant framework that could enrich the multiple transformative constituencies that challenge the industrial food system and therefore facilitate the convergence of movements that reject the commodification of food.

The preparation of gold nanoparticles (AuNPs) involves a variety of chemical and physical methods. These methods use toxic and environmentally harmful chemicals. Consequently, the synthesis of AuNPs using green chemistry has been under investigation to develop eco-friendly nanoparticles. One method to achieve this is the use of plant-derived phytochemicals capable of reducing gold ions to produce AuNPs. The aim of this study was to implement a facile microtitre-plate method to screen a large number of aqueous plant extracts to determine the optimum concentration (OC) to bio-synthesize the AuNPs. Several AuNPs of different sizes and shapes were successfully synthesized and characterized from seventeen South African plants. The characterization was done using Ultra Violet-Visible Spectroscopy, Dynamic Light Scattering, High Resolution Transmission Electron Microscopy and Energy-Dispersive X-ray Spectroscopy. We also studied the effects of temperature on the synthesis of the nanoparticles and measured its effect on the particle size of the synthesized AuNPs and the data showed that changes in temperatures affect the size and dispersity of the generated AuNPs. Further, some of the synthesized AuNPs were stable upon incubation with different biological solutions in vitro.

The increase in ecosystem biodiversity can be perceived as one of the universal processes converting energy into information across a wide range of living systems. This study delves into the dynamics of living systems, highlighting the distinction between ex-post adaptation, typically associated with natural selection, and its proactive counterpart, ex-ante adaptability. Through coalescence experiments using synthetic ecosystems, we (i) quantified an ecosystem stability, (ii) identified correlations between species richness and the stability, (iii) proposed a mechanism for increasing biodiversity through moderate inter-ecosystem interactions, and (iv) inferred that the information carrier of ecosystems is species composition, or merged genomic information. Additionally, we observed that (v) changes in ecosystems are constrained to a low-dimensional state space, with three distinct alteration trajectories—fluctuations, rapid environmental responses, and long-term changes—converging into this state space in common. These findings suggest that daily fluctuations may predict broader ecosystem changes. Our experimental insights, coupled with an exploration of living systems' information dynamics from an ecosystem perspective, enhance our predictive capabilities for natural ecosystem behavior, providing a universal framework for understanding a broad spectrum of living systems.

The western Himalayan region is a biodiversity hotspot. Although many of the flora and fauna are documented, there are very few studies of the fungal diversity. Here, we present the statistical analyses of the specimen collections from the past 150 years of data in the fungarium of the Forest Research Institute. We found that the host tree species, pine, oak, deodar, and spruce, had very high Shannon diversity (SD) and species richness (SR), while Dalbergia and Rhododendron showed moderate to low SD, SR; Sal, although found at lower altitudes has high SD, SR. Among the fungal families, the highest SD, SR was found in Polyporaceae. Hymenochaetaceae, Peniophoraceae, Coleosporiaceae, and Stereaceae also showed SD with moderate SR. Fomitopsidaceae, Cronartiaceae, Ganodermataceae, and Thelephoraceae have low species diversity and species. Thelesporaceae are distributed above 2000 m altitude, and Ganodermateceae and Hymenochaetaceae have broader ranges, namely 0 - 3500 m and 0 - 5000 m, respectively. The Polyporaceae show diverse variations in species distribution, occurring between 0 and 4500 m; Colesporaceae and Cronartiaceae around 2000 m; and, Stereaceae, Meruliaceae, Peniophoraceae, and Fomitopsidaceae occur between 1700 and 1800 m, with all Stereaceae and Fomitopsidaceae having different distribution ranges up to 5000 m. We found that areas with relatively low rainfall had lower species richness, and vice versa, and high solar radiation also negatively impacted fungal density and SR. Similar studies must be undertaken in other parts of the Himalayas, and the importance of fungi in ethnobotanical needs to be understood for ensuring sustainable use.

Maldivian coral reefs have been experiencing significant degradation due to a combination of global climate change and local anthropogenic pressures. To enforce the conservation of coral reefs worldwide, coral restoration is becoming a popular tool to restore ecosystems actively. In the Maldives, restoration interventions are performed only around touristic islands, where there are economic resources available to support these projects. Unfortunately, on local islands, coral restoration does not benefit from the same support and is rarely boosted. A challenging coral restoration intervention has been performed, for the first time, a on a local island of the Maldives affected by intense human pressures that caused the degradation of its reefs. A total of 242 coral fragments were collected from impacted colonies and transferred to the coral nursery of the island. Survival and growth rates of the fragments were monitored for 12 months. After one year, a survival rate of 70.2% was recorded. Although this rate might appear lower when compared to other restoration experiences, it is very promising considering the origin of the fragments and the poor quality of the environment where they have been transplanted. Some potential threats to the success of this restoration have also been identified, i.e., water temperature anomaly, diseases and parasites, the latter being the leading causes of coral mortality. The procedure presented here is comparatively less expensive than the typical relocation of entire coral colonies from donor healthy reefs to degraded reefs, thus providing an opportunity and a viable option also for local islands to restore their reefs and preserve local biodiversity.

Rich biodiversity is one of the Philippines’ greatest assets. Unfortunately, there is a continuous decline in the diversity of flora and fauna across the world. This calls for the need to educate people, especially younger generations, to value and protect biodiversity and natural resources. The study aimed to assess the students’ extent of knowledge and identify their perspectives towards biodiversity and its protection and conservation. A total of 268 randomly selected students at Aurora State College of Technology Zabali Campus were involved in the study. Survey questionnaires were used to obtain data and information which were subjected to statistical tests. The students had a moderate knowledge level on biodiversity with a mean score of 6.65 out of 10 items (SD = 1.50). Their perspective on biodiversity was leaning toward its protection and conservation, with a mean score of 7.2 out of 10 items (SD = 1.29). Factors affecting the students’ knowledge were gender (p = .003) and academic department (p = 0.003). Females and those associated with the Department of Forestry and Environmental Sciences and Department of Industrial technology were found to have more knowledge than the others. Males, on the other hand, were found to have a more positive perspective towards biodiversity. Knowledge and perspective had a weak correlation with r = 0.39. Students were not well-aware, but were in support of the Philippines’ biodiversity-related laws, which could help shape their mindset and actions towards biodiversity conservation and protection. As an implication, the college administration must revisit the curricula of all degree programs and ensure that students from each degree program are environmentally educated, emphasizing biodiversity conservation.

Cyanobacterial species (blue-green algae) constitute the major part of the phytoplanktonic biomass during the summer in freshwater ponds. The aim of the research work was to study the biodiversity of cyanobacteria among 20 different freshwater ponds of the Pudukkottai district of Tamil Nadu, India. The morphological identification of cyanobacterial species was carried out using a trinocular microscope. The results showed that the maximum number of cyanobacterial species belonged to Oscillatoriaceae, Nostocaceae, Microcystaceae, Scenedesmaceae, and Desmidiaceae families. Among 25 different families of Cyanobacteria about 42 distinct species were identified. These results showed that the freshwater ponds of the Pudukkottai district have an abundance of cyanobacteria species.

Measuring the status and trends of biodiversity is critical for making informed decisions about the conservation, management or restoration of species, habitats and ecosystems. Defining the reference state against which status and change are measured is essential. Typically, reference states describe historical conditions, yet historical conditions are challenging to quantify, may be difficult to falsify, and may no longer be an attainable target in a contemporary ecosystem. We have constructed a conceptual framework to help inform thinking and discussion around the philosophical underpinnings of reference states and guide their application. We characterise currently recognised historical reference states and describe them as Pre-Human, Indigenous Cultural, Pre-Intensification and Hybrid-Historical. We extend the conceptual framework to include contemporary reference states as an alternative theoretical perspective. The contemporary reference state framework is a major conceptual shift that focuses on current ecological patterns and identifies areas with higher biodiversity values, regardless of the disturbance history. The specific context for which we design the contemporary conceptual frame is underpinned by an overarching goal—to maximise biodiversity conservation and restoration outcomes in existing ecosystems. The contemporary reference state framework can account for the inherent differences in the diversity of biodiversity values (e.g., species richness, habitat complexity) across spatial scales, communities and ecosystems. In contrast to historical reference states, contemporary references states are measurable and falsifiable. This ‘road map of reference states’ offers perspective needed to define and assess the status and trends in biodiversity and habitats. Our framework for contemporary reference states provides a tractable way for policy-makers and practitioners to navigate biodiversity assessments to maximise conservation and restoration outcomes in contemporary ecosystems. We illustrate how to define a contemporary reference state using an example from south-eastern Australia.

Despite their limited spatial extent, freshwater ecosystems host remarkable biodiversity, including one third of all vertebrate species. This biodiversity is declining dramatically: globally, wetlands are vanishing three times faster than forests and freshwater vertebrate populations have fallen more than twice as steeply as terrestrial or marine populations. Threats to freshwater biodiversity are well- documented but co-ordinated action to reverse this decline is lacking. We present an Emergency Recovery Plan to “bend the curve” of freshwater biodiversity loss. Priorities for action include: 1) accelerating implementation of environmental flows, 2) improving water quality, 3) protecting and restoring critical habitats, 4) managing exploitation of freshwater species and riverine aggregates, 5) preventing and controlling non-native species invasions, and 6) safeguarding and restoring river connectivity. We recommend revised targets and indicators for the Convention on Biological Diversity and the Sustainable Development Goals, and investment in enabling conditions at national, river basin and local scales.

Forest canopy gaps are important for the ecosystem dynamics. Depending on tree species, small canopy openings might be also associated to intra-crown porosity and to space between crowns. Yet, little is known on the relationships between the fine-scaled pattern of canopy openings and biodiversity features. This research explored the possibility of i)- mapping forest canopy gaps from a very high resolution orthomosaic (10 cm), processed from a versatile imaging platform such as unmanned aerial vehicles (UAV), ii)- to derive patch metrics that can be tested as covariates of variables of interest for forest biodiversity monitoring. This is attempted in a test area of 240 ha covered by temperate deciduous forest types in Central Italy and containing 50 forest inventory plots of about 530 m². Correlation and linear regression techniques were used to explore relationships between patch metrics and understorey (density, development and species diversity) or forest habitat biodiversity variables (density of micro-habitat bearing trees, vertical species profile, tree species diversity). The results revealed that small openings in the canopy cover (75% smaller than 7 m²) can be faithfully extracted from UAV RGB imagery, using the red band and contrast split segmentation. Highest correlations were observed in the mixed forest (beech and turkey oak), while beech forest had the poorest ones and turkey oak forest displayed intermediate results. Moderate to strong linear relationships were found between gap metrics and understorey variables in mixed forest type, with adjusted R² from linear regression ranging from 0.52 to 0.87. Equally good results, in the same forest types, were observed for forest habitat biodiversity variables (0.52<adjusted R²<0.79) with highest values found for density of trees with microhabitats and vertical species profile. In conclusion, this research highlights that UAV remote sensing can potentially provide covariate surfaces of variables of interest for forest biodiversity monitoring, conventionally collected in forest inventory plots. By integrating the two sources of data, these variables can be mapped over small forest areas with satisfactory levels of accuracy, at a much higher spatial resolution than would be possible by field-based forest inventory solely.

Urban lakes, especially those of natural origin, provide ecosystem services, recreation being one of the most important and highly valued by the city dwellers. Fulfilling the needs of city residents to relax and have contact with nature has become a priority in urbanized areas and has been proved to positively affect people’s health and well-being. The recreational potential of water bodies was identified to be most important aspect of ecosystem services to the residents of the neighboring areas. An assessment of recreational ecosystem services (RES) provisioning to society based on the real time spent by the citizens and housing values in the rural-urban gradient revealed that the economic benefits of lakes differ in urbanized, suburban and rural landscapes. The growth of cities has led to an increased population density in the surroundings of ecologically valuable areas, resulting in higher pressure from visitors seeking recreational areas. Along with urbanization, the impoverishment of ecosystem functions takes place, limiting their capability to provide ecosystem services. In this work provisioning of recreational ecosystem services of 28 floodplain lakes located along the urban-rural gradient of the Warsaw agglomeration was assessed. The relationship between the ecological value of the water bodies, measured using naturalness indices, and the recreational ecosystem services they can provide was assessed. The results showed that the floodplain lakes located along the urban-rural gradient are of a great importance to the citizens due to their recreational potential. The provisioning of recreational ecosystem services is poorly connected with the ecological characteristics of the floodplain lakes. Only hemeroby, was significantly correlated with provisioning, and there was no relationship with factors such as naturalness of vegetation or water quality, demonstrating that public preference was not generally influenced by high ecological quality. These data should be available to potential buyers and be integrated in spatial planning management plans in order to shape future housing policy.

Extensive green roofs provide for many ecosystem services in urban environments. The efficacy of these services is influenced by the substrate characteristics and vegetational composition. Despite their key role in plant performance and productivity, or their contribution to nitrogen fixation or carbon sequestration, green roof microbial communities have received little attention so far. Here, we investigated whether microbial communities in extensive green roof substrates contain a core community, as well as whether they are affected by green roof plant diversity. Eleven green roofs, planted either with a mixture of grasses, wildflowers and succulents (Sedum spp.), or solely species of Sedum, were seasonally sampled to investigate prokaryotic and fungal communities via metabarcoding. Although Sedum-herbs-grasses roofs contained more plant species, we observed no discriminant microbial communities between both roof types, likely due to sharing the same substrate textures and the vegetational layers that became more similar throughout time. Examining key microbial taxa revealed that most taxa are also dominant phylotypes in soils across different biomes. Many bacterial core taxa are capable of nitrogen fixation and most fungal key taxa are stress-tolerant saprotrophs, endophytes, or both. Microbial community composition further revealed high similarities with aerial samples taken in urban environments. Considering the extreme edaphic conditions that periodically arise in green roof substrates, further investigation is warranted to determine the extent to which the dynamic and intriguing microbial communities are influenced by the aeromicrobiome.

Biodiversity conservation is a key factor in meeting sustainable development goals. This is even more important in cities, where green spaces are becoming increasingly scarce. This study analyzes the Kinshasa's proliferating ornamental plant nurseries, known as informal horticultural sites (IHS). The analysis focused on characterizing the profile of horticulturists, their production conditions and the ornamental species produced. Fifteen IHSs were sampled using the "snowball" technique, and 178 horticulturists were surveyed. Based on the socio-professional profile of the horticulturists, five groups of IHS are distinguished after a hierarchical classification on principal components. We found that IHSs exclusively employed men, most of whom were new to the trade, from all levels of education, and most of whom ranged from 19 to 45 years old. Production conditions are relatively similar from one site to another. However, all IHS are characterized by permanent land insecurity, the use of phytosanitary products, plant conditioning methods that are not very diversified and calibrated to growers' investment capacities, and diversified seed acquisition methods. A total of 139 ornamental species, most of them exotic, were identified. Of these, 37% are phanerophytes and 24% are considered potentially invasive. We suggest ways of professionalizing the activity and protecting the urban environment.

The high incidence of psychopathologies recorded in today's human society, correlated with the high percentages of biodiversity loss, point to the need for an interdisciplinary approach of the scientific fields under study – Neurosciences and Biodiversity Conservation. Thus, our approach here pre-sents, in a synergistic manner, the significant correlation between mental health and the increased values of biodiversity in the ecosystems located in the immediate vicinity, especially those located in the middle of cities. Our approach aims to emphasize the importance of biodiversity conservation in the context of preserving mental health and general well-being. There are a series of recent ex-perimental demonstrations that outline the influence of natural elements on the human psyche and implicitly the effects of nature in the prevention and reduction of stress, anxiety and depression. And beyond the cognitive barriers of humanity in relating to the surrounding biodiversity must lie the desire to know the values of biodiversity and the absolute importance of its conservation. The sustainable relationship between man and living nature, seen as a complex of biodiversity, is dealt with by a branch of science called Human Ecology. Therefore, this study emphasizes the crucial need to know and respect the connection between man and nature, based, since time immemorial, on biophilia. And with the regression of ignorance and the correlated approach of several scientific fields, some at the intersection of humanities and natural sciences, one can observe the progress of preserving the dynamic balance within the ecosystems and implicitly the preservation of mental health and human well-being.

The biodiversity loss in urban areas has attracted public concern, which is one of the urgent global environmental issues. This study used bibliometric methods to analyze 3351 publications from 1995-2021 that retrieved from the Web of Science and visually represented the state-of-the-art of researches in city biodiversity field. The prolific authors, journals(sources), institutions, and countries are clearly identified. The most cited and influential paper proposed a conceptual framework of associations between urban green space, and ecosystem and human health, and then concluded that green infrastructure could physically and psychologically benefits people by ecosystem services it provides, and make a better socio-economic benefit. The theme hotspots are urbanization, urban ecology, ecosystem services, urban planning, green infrastructure, urban forest and urban park et al. Ingo Kowarik is the most productive author in terms of number of publications. Michael L. McKinney is the most cited authors by the publications in analyzed corpus, who identified how urbanization harms native ecosystems, however, a well ecologically educated population could greatly enhance species richness in all ecosystems. Urban park, garden, fragmented green spaces and green corridor networks could help enhance city biodiversity. In general, the city biodiversity research presents a trend that involves intensive global cooperation, and become more comprehensive.

Biodiversity loss has been identified as one of the environmental impacts where humankind has been tres-passing planetary boundaries most significantly. Going beyond the pressures causing damages (calling them ‘direct drivers’) and analysing their underlying driving forces, IPBES, the Intergovernmental Sci-ence-Policy Platform for Biodiversity and Ecosystem Services, also identified a series of indirect drivers. The Montreal-Kunming Global Biodiversity Framework GBF including its suggested monitoring approach is intended to and claims to be a policy response to such analyses. However, to assess the human impact on ecosystems as a basis for planning conservation and restoration, as foreseen in the GBF, monitoring ecosystem typologies (in the GBF with reference to the UN statistical standard SEEA ES, which in turn refers to the IUCN ecosystem classification) is not enough. It needs to be complemented with data on the severity of human impacts, and on the history of places, i.e. how and when the current ecosystem status was brought about. In this conceptual paper we suggest LUI, a deliberately simple ordinal scale index for land use intensity changes, to address these two gaps. It is based on the hemeroby concept, measuring the human impact as deviation from naturalness. This makes it an information collection and presentation tool for those working in landscape planning and management. LUI’s simple and intuitively understandable structure makes it suitable for citizens’ science applications, and thus for participative monitoring when extensive statistical data gathering is not feasible, and past data are not available. Of course is can also be used as a simple too for communicating when detailed statistical data series are available. While the aggregate index is expected to communicate well, its components are more relevant to motivate and help policy makers to prioritise their decisions according to the severity of recent anthropogenic ecosystem disturabances.

Mounting evidence supports the connections between exposure to environment types––such as green spaces and biodiversity––and human health. However, the mechanistic links that connect biodiversity (the variety of life) and human health, plus the level of supporting evidence, are less clear. Here, we undertook a scoping review to map the links between biodiversity and human health and summarise the levels of associated evidence using an established weight of evidence framework. Distinct from other reviews, we provide additional context regarding the environment-microbiome-health axis, evaluate the environmental buffering pathway (e.g., biodiversity impacts on air pollution), and draw upon expert opinion to provide case studies on three underrepresented linkages. The case studies include (1) biodiversity and Indigenous Peoples’ health, (2) biodiversity and urban social equity, and (3) biodiversity and COVID-19. We observed a moderate level of evidence to support the environmental microbiota-human health pathway and a moderate-high level of evidence to support broader nature pathways (e.g., green space) to various health outcomes, from stress reduction to enhanced wellbeing and improved social cohesion. However, studies of broader nature pathways did not typically include specific biodiversity metrics, indicating clear research gaps. Further research is required to understand the connections and causative pathways between biodiversity (e.g., using metrics such as taxonomy, diversity/richness, structure, and function) and health outcomes. There are well-established frameworks to assess the effects of broad classifications of nature on human health. These can assist future research in linking biodiversity metrics to human health outcomes. Our case studies on underrepresented linkages highlight the roles of biodiversity and its loss on urban lived experiences, infectious diseases, and Indigenous Peoples’ sovereignty and livelihoods. More research and awareness of these socioecological interconnections are needed.

There is a sustained rise in incidence of cancer and toxicity related to chemicals exerting enormous burden to public health and biodiversity. Polycyclic Aromatic Hydrocarbons (PAHs) are mong such contaminants, precisely the sixteen-priority characterized by United States Environmental Protection Agency (USEPA). Therefore, this review is aimed at further elaboration about the 16 USEPA characterized PAHs and threat portend to public health and biodiversity. PAHs are a class of very stable organic pollutants produced most commonly, by incomplete combustion of fossil fuel and are formed when complex organic substances are exposed to heat. PAHs in great amount due to build up over time by bioaccumulation can be perilous: to human beings of all age and levels, aquatic organisms, amphibians and reptiles. The soil like the aquatic environment contains substantial quantity of PAHs since, atmospheric PAHs sediments on the soil due to dry and wet deposition, terrestrial organism are impacted if the soil is saturated with PAHs. Therefore, PAHs are a great source of trepidation for food safety, public health and biodiversity sustenance. Hence, tackling the spade of the menacing ubiquity of PAHs becomes necessary from its sources by encouragement of alternatives to petroleum fuels for machines and vehicles.

Questions: Elevation, biodiversity, and forest structure are commonly correlated, but their relationships near the positive extremes of biodiversity and elevation are unclear. We asked 1) How does forest structure vary with elevation in a high biodiversity, high topographic complexity region? 2) Does forest structure predict vascular plant biodiversity? 3) Is plant biodiversity more strongly related to elevation or to forest structure? Location: Great Smoky Mountains National Park, USAMethods: We used terrestrial LiDAR scanning (TLS) to characterize vegetation structure in 12 forest plots. We combined two new canopy structural complexity metrics with traditional TLS-derived forest structural metrics and vascular plant biodiversity data to investigate correlations among forest structure metrics, biodiversity, and elevation. Results: Forest structure varied widely across plots spanning the elevational range of GRSM. Our new measures of canopy density (Depth) and structural complexity (σDepth) were sensitive to structural variations and effectively summarized horizontal and vertical dimensions of structural complexity. Vascular plant biodiversity was negatively correlated with elevation, and more strongly positively correlated with vegetation structure variables. Conclusions: The strong correlations we observed between canopy structural complexity and biodiversity suggest that structural complexity metrics could be used to assay plant biodiversity over large areas in concert with airborne and spaceborne platforms.

Population genetic data underpin many studies of behavioral, ecological, and evolutionary processes in wild populations and contribute to effective conservation management. However, collecting genetic samples can be challenging when working with endangered, invasive, or cryptic species. Environmental DNA (eDNA) offers a way to sample genetic material non-invasively without requiring visual observation. While eDNA has been trialed extensively as a biodiversity and biosecurity monitoring tool with a strong taxonomic focus, it has yet to be fully explored as a means for obtaining population genetic information. Here, we review current research that employs eDNA approaches for the study of populations. We outline challenges facing eDNA-based population genetic methodologies, and suggest avenues of research for future developments. We advocate that with further optimizations, this emergent field holds great potential as part of the population genetics toolkit.

A free-fall baited camera lander was launched for the first time on the Cosmonaut Sea shelf of East Antarctica at the depth of 694 m during the 38th Chinese National Antarctic Research Expedition (CHINARE) in 2022. We identified 31 unique taxa (23 of invertebrates and 8 of fishes) belonging to 8 phyla from 2403 pictures and 40 videos. The Antarctic jonasfish (Notolepis coatsi) was the most frequently observed fish taxa. Ten species of vulnerable marine ecosystem (VME) taxa were observed, accounting for 32% of all species. The maximum number (MaxN) of Natatolana meridionalis individuals per image frame was ten, and they were attracted to the bait. The macrobenthic community type was sessile suspension feeders with associated fauna (SSFA), which was shaped by the muddy substrata with scattered rocks. Rocks served as the best habitats for sessile fauna. The study reveals the megafauna community and their habitat by image survey in the Cosmonaut Sea for the first time. It helped us obtain Antarctic biodiversity baselines and monitoring data for future’s ecosystem health assessment and better protection.

DNA barcoding has emerged as a transformative tool in the realm of biodiversity assessment, facilitating rapid and precise species identification. This review article delves into the evolving landscape of DNA barcoding, with a particular focus on the emergence of mini-barcodes and alternative genes as innovative approaches. Traditional DNA barcoding, anchored in the COI gene, has significantly advanced our understanding of species diversity; however, inherent challenges persist, especially when dealing with degraded samples or taxonomic groups where COI falls short. The introduction of mini-barcodes, characterized by their reduced sequence length, has revolutionized DNA barcoding by expanding its applicability. We explore the utility of mini-barcodes in various scenarios, ranging from DNA extraction from ancient or fragmented specimens to in-field identification using portable sequencing technologies. Case studies and practical insights into their effectiveness are presented, emphasizing their role in overcoming traditional limitations. Alternative genes, a complementary facet of DNA barcoding, offer a novel avenue for enhancing taxonomic resolution and accuracy. This review showcases instances where researchers have harnessed alternative genes to overcome COI's constraints, particularly in diverse taxonomic groups, such as fungi, plants, and microorganisms. The selection of these genes is discussed, along with their potential to uncover hidden biodiversity and improve species delimitation. Examining the broader implications of mini-barcodes and alternative genes, we unveil their pivotal roles in advancing conservation efforts, forensic science, and citizen science initiatives. The adaptable nature of these approaches empowers a diverse range of stakeholders, from scientists conducting critical research to concerned citizens contributing to our collective understanding of the natural world. Looking ahead, we envision an exciting future for DNA barcoding, as these innovations continue to evolve. Integration with cutting-edge sequencing technologies, data analysis methods, and the harnessing of big data hold immense potential. Nonetheless, challenges related to standardization, scalability, and data sharing persist.

A burgeoning body of scholarship identifies and discusses botanic gardens as increasingly important centres of biodiversity conservation and sustainability. Notwithstanding the high quality and richness of this literature, it encompasses relatively autonomous fields of expertise that are neither designed nor expected to promote cross-disciplinary, integrative, accounts of botanic gardens as institutions of conservation and sustainability. Bridging key aspects of the botanic garden literature, this article brings into conversation historical accounts with contemporary scholarship on the matter. In so doing, it unveils dilemmas and challenges faced by botanic gardens as they grapple with their historic legacies, but also renew their relevance in nurturing sustainable socio-environmental futures. The article proceeds by covering three focal points. First, it summarizes the scholarly literature on the history of botanic gardens. Second, it presents accounts of current scientific and biodiversity conservation endeavours as reflexive engagements with their historical legacies. Third, it addresses the recent emergence of socio-cultural missions at botanic gardens as a significant step beyond their historical focus on plants and plant ecosystems.

The South of France is a biodiversity hotspot within Europe. Here, we present a comprehensive review of surveys conducted in the Cent Fonts aquifer, an overlooked subterranean biodiversity hotspot embedded in a region rich in stygobiotic species and threatened by climate change and water abstraction projects. Key studies, spanning from 1950 to 2006, show a progression in survey methods and results, although troglobiotic species remain poorly documented. With 43 stygobiotic species recorded, the Cent Fonts is the second richest stygobiont hotspot in Europe. Most species are regional endemics, a quarter of which are considered vulnerable by the IUCN. The Cent Fonts also hosts several relict species and is the type locality of four species. Such a high biological value clearly deserves to be preserved. Our analysis warns of a possible decline in biodiversity, as 8 of the species recorded in the 20th century were absent from the 2006 survey, suggesting potential threats of unknown origin. The capture of the Cent Font springs for water abstraction is discussed as a potential threat to this ecosystem and its unique biodiversity. Three new species of stygobiotic molluscs are described, one of which was collected in the Cent Fonts.

Infections with symbiotic single-celled organisms classified as Ciliophora, are commonly reported in various species of invertebrates. Freshwater mussels also play the role of a host for them, being responsible for their biodiversity. Knowledge about these relationships is insufficient. Freshwater mussels are among the most endangered groups of animals. Ciliate endosymbionts in mussels may be responsible for the diseases and increasingly frequent cases of mass mussel deaths and are particularly dangerous for commercially farmed mussels and for restocking of mussels as part of active conservation measures. On the other hand the Ciliophora parasites and commensals living in freshwater mussels is undescribed. Many of these species are likely to become extinct before they are known to science. Recently, two genera of Ciliophora, Conchophthirus sp. and Trichodina sp., have been described in the mantle cavity of U. crassus - an endangered and protected species in the EU. The basis of extensive research on the impact of endosymbiotic ciliates on U. crassus populations is the knowledge of their species composition, proportion of infected individuals, level of infection intensity and distribution in different types of rivers. Such studies were carried out in the mantle cavity of U. crassus from three rivers in three seasons. Cloning, sequencing and functional analysis of the genetic material of Ciliophora was carried out using NGS (Next-Generation Sequencing) analysis based on the hypervariable V4 and V9 regions of the 18SrRNA gene, which enables the identification of taxonomic groups, including genera and species. The most numerous OTUs are common and cosmopolitan species. But some commensals and potentially parasites were found too. Ciliophora associated with mantle cavity form an interesting, hierarchical biocomplex but their interactions with mussels need further studies.

The agro-biodiversity present in agro-ecosystems is fundamental in guaranteeing sustainability and resilience. However, there are very few proposals for evaluating it and, even less, ones that include indicators to analyze the influence of the structural and spatial configuration of a landscape in order to favor agro-biodiversity connectivity to productive systems, management and conservation practices, and the producer (farmer)’s perceptions, awareness and ability to favor it on his farm. The Main Agro-ecological Structure MAS is redefined as an environmental agro-biodiversity index. New indicators are established for a total of 10 criteria and 29 indicators of systems that describe the agro-biodiversity of agro-ecosystems. Methodologies for its evaluation are also described, and the possibility of adapting certain indicators according to the ecological and cultural contexts where the farms are located is discussed.

The ongoing exponential growth of human population poses a risk to sustainable development goals (SDGs). Unless we understand the drivers of this growth and inform policy development accordingly, SDGs would remain a dream. One of the old theories of population growth known as the Malthusian theory predicts that resource availability drives population growth to a certain time when population growth outrun resource availability, leading to all sort of crises known as Malthusian crisis. Although the link between economic growth and population has been widely investigated while testing the theory, little is known about environmental and social factors po-tentially driving population growth. Here, because of various crises of our time recalling the Malthusian crisis, we revisited the theory by fitting structural equation models to environmental, social and economic data collected over 30-year period in South Africa. None of the social varia-bles tested predicts population growth. Instead, we found that biodiversity (species protection index) correlates positively with population growth. Biodiversity provides various resources through ecosystem goods and services to human, thus supporting population growth as pre-dicted in the Malthusian theory. However, we also found that this population growth may lead to conservation conflict as we found that biodiversity habitat (wetland area) correlates negatively with population growth, thus raising the compromising effect of population growth on life on earth. What’s more, we found a significant link between economic growth measured as GDP and population growth, further supporting the Malthusian prediction. Overall, our study re-affirms the value of biodiversity to human and suggests that the Malthusian theory should continuously be tested with predictors other than economic.

Nature-based solutions (NbS) are increasingly recognised for their potential to address both the climate and biodiversity crises. These outcomes are interdependent, and both rely on the capacity of NbS to support and enhance the health of an ecosystem: its biodiversity, the condition of its abiotic and biotic elements, and its capacity to function normally despite environmental change. However, while understanding of ecosystem health outcomes of nature-based interventions for climate change mitigation is growing, the outcomes of those implemented for adaptation remain poorly understood with evidence scattered across multiple disciplines. To address this, we conducted a systematic review of the outcomes of 109 nature-based interventions for climate change adaptation using 33 indicators of ecosystem health across eight broad categories (e.g. diversity, biomass, ecosystem functioning and population dynamics). We showed that 88% of interventions with positive outcomes for climate change adaptation also reported measurable benefits for ecosystem health. We also showed that interventions were associated with a 67% average increase in local species richness. All eight studies that reported benefits in terms of both climate change mitigation and adaptation also supported ecosystem health, leading to a triple win. However, there were also trade-offs, mainly for forest management and creation of novel ecosystems such as monoculture plantations of non-native species. Our review highlights two major limitations of research to date. First, only a limited selection of metrics are used to assess ecosystem health and these rarely include key aspects such as functional diversity and habitat connectivity. Second, taxonomic coverage is poor: 67% of outcomes assessed only plants and 57% did not distinguish between native and non-native species. Future research addressing these issues will allow the design and adaptive management of NbS to support healthy and resilient ecosystems, and thereby enhance their effectiveness for meeting both climate and biodiversity targets.

Industrial agriculture (IA) is the predominant model of food production since the Green Revolution in the 1950s. IA has been recognized among the main drivers of biodiversity loss, climate change and native pollinator decline. This is controversial, given that native agricultural pollinators are an important resource biota already contributing to crop yield, especially in areas defined as "world biodiversity hotspots” (WBH). These areas often overlap with agricultural zones hosting a significant proportion of cultivated land, mainly through intensive agricultural practices. Pollinator biodiversity and pollination services in these places are currently under threat due to the negative consequences of IA. The dual role of insects as key players allowing the maintenance of the natural ecosystem, as well as main crop pollinators, is particularly exacerbated and urgently requires conservation actions in WBH and food-producing zones. Here we summarize the known negative effects of IA on pollinator biodiversity and illustrate these problems by considering the case of Chile. Food exports represent a considerable part of the economy in this OECD “developing country” in the “Global South”, and a large part of its surface has been highlighted as a unique WBH. This area is currently being replaced by IA businesses at a fast pace, threatening local biodiversity. We present agroecological strategies for sustainable food production and pollinator conservation in food-producing WBHs like Chile. These alternatives recognize native pollinators as internal inputs that cannot be replaced by IA technological packages or external inputs and support the development of agroecological and biodiversity restoration practices to protect their existing biodiversity. We suggest a strategy that integrates four fundamental pillars for producing food in a sustainable way, recognizing biodiversity and local cultural heritage: 1) Share the land, restore and protect; 2) Ecological intensification; 3) Localized knowledge, research and technological development; and 4) Territorial planning and implementation of socio-agroecological policies. We suggest that this approach does not need greater modification of native pollination services that sustain the world with food and basic subsistence goods, but a paradigm change where the interdependency of nature and human wellbeing are recognized for ensuring the present and future of the world’s food security and sovereignty as well as considering the reduction of consumerism and food waste.

Global climate change requires urgent solutions. Ambitious tree-planting initiatives, many already underway, aim to sequester enormous quantities of carbon, partly compensating for the anthropogenic CO₂ emissions that are a major cause of rising global temperatures. However, poorly planned and executed tree-planting could actually increase CO₂ emissions and have long-term, deleterious impacts on biodiversity, landscapes and livelihoods. Here, we highlight the main environmental risks of large-scale tree planting and propose ten golden rules, based on some of the most recent ecological research, to implement forest ecosystem restoration that maximizes rates of both carbon sequestration and biodiversity recovery, while simultaneously improving livelihoods. These are: i) Protect existing forest first; ii) Work together (involving all stakeholders); iii) Maximize biodiversity recovery to meet multiple goals; iv) Select appropriate areas; v) Use natural regeneration wherever possible; vi) Select species to maximise biodiversity; vii) Use resilient plant material (with appropriate genetic variability and provenance); viii) Plan ahead for infrastructure, capacity and seed supply; ix) Learn by doing (using an adaptive management approach); and x) Make it pay (ensuring the economic sustainability of the project). We focus on the design of long-term strategies to tackle the climate and biodiversity crises and support livelihood needs. We emphasize the role of local communities and their dependence on benefits from successful reforestation programmes that restore ecosystem functioning and deliver a diverse range of forest products and services. While there is no simple and universal recipe for forest restoration, it is now crucial to build on the public and private interest in this topic to ensure interventions provide effective, long-term carbon sinks and maximise benefits for biodiversity and people.

In 2010, Parties to the Convention on Biological Diversity (CBD) adopted the Strategic Plan for Biodiversity 2011–2020 to address the loss and degradation of nature. Subsequently, most biodiversity indicators continued to decline. Nevertheless, conservation actions can make a positive difference for biodiversity. The emerging Post-2020 Global Biodiversity Framework has potential to catalyze efforts to ‘bend the curve’ of biodiversity loss. Thus, the inclusion of a goal on species, articulated as Goal B in the Zero Draft of the Post-2020 Framework, is essential. However, as currently formulated, this goal is inadequate for preventing extinctions, and reversing population declines; both of which are required to achieve the CBD’s 2030 mission. We contend it is unacceptable that Goal B could be met while most threatened species deteriorated in status and many avoidable species extinctions occurred. We examine the limitations of the current wording and propose an articulation with robust scientific basis. A goal for species that strives to end extinctions and recover populations of all species that have experienced population declines, and especially those at risk of extinction, would help to align actors towards the transformative actions and interventions needed for humans to live in harmony with nature.

The current focus on afforestation in climate policy runs the risk of compromising both longterm carbon storage and human adaptation. It also works against efforts to stem the tide of biodiversity loss. We outline why an emphasis on diverse, intact natural ecosystems—as opposed to tree plantations with fast-growing exotic species—will help nations deliver the goals of the Paris Agreement and much more.

The Baculoviridae, a family of insect-specific large DNA viruses, is widely used in both biotechnology and biological control. Its applied value stems from millions of years of evolution influenced by interactions with their hosts and the environment. To understand how ecological interactions, have shaped baculovirus diversification, we reconstructed a robust molecular phylogeny using 217 complete genomes and ~580 isolates for which at least one of four lepidopteran core genes was available. We then used a phylogenetic-concept-based approach (mPTP) to delimit 165 baculovirus species, including 38 species derived from new genetic data. Phylogenetic optimization of ecological characters revealed a general pattern of host conservatism punctuated by occasional shifts between closely related hosts and major shifts between lepidopteran superfamilies. Moreover, we found significant phylogenetic conservatism between baculoviruses and the type of plant growth (woody or herbaceous) associated with their insect hosts. In addition, we found that colonization of new ecological niches sometimes led to viral radiation. These macroevolutionary patterns show that besides selection during the infection process, baculovirus diversification was influenced by tritrophic interactions, explained by their persistence on plants and interactions in the midgut during horizontal transmission. This complete eco-evolutionary framework highlights the potential innovations that could still be harnessed from the diversity of baculoviruses.

The consequences of climate change and anthropogenic stressors, such as habitat loss and overexploitation, are threatening the subsistence of species and communities across the planet. Therefore, it is crucial to analyze the impact of environmental perturbations on the diversity, structure and function of ecosystems. In this work, in silico simulations of biodiversity loss were carried out on the marine food web of Caleta Potter (25 de Mayo Island, Antarctica) where global warming has caused critical changes in the abundance and distribution of benthic and pelagic communities during the last 30 years. We performed species removal considering their degree and trophic level and including four different thresholds on the occurrence of secondary extinctions. We examined the impact of extinctions on connectance, modularity and stability of the food web. We found different responses of these properties depending on the extinction criteria used, e.g., large increase in modularity and rapid decrease in stability when most-connected and relatively high-trophic level species were removed. Additionally, we studied the complexity-stability relationship of the food web founding two regimes: 1) high sensitivity to small perturbations suggesting that Potter Cove would be locally unstable, and 2) high persistence to long-range perturbations suggesting global stability of this ecosystem.

Fresh water is an increasingly scarce resource in both urban and rural developments. As a response to this challenge, non-potable water reuse is on the rise. This research explored a potential off-grid system for water purification, consisting of a staircase wetland with terracotta pot plants working as a filter for greywater. The study further investigated the physicochemical properties of the greywater and the soil before and after the wetland purification. Results showed that the filtered water satisfied all requirements for water reuse, e.g., pH, turbidity, and total coliforms. The research then uniquely investigated the effect of greywater on the soil biodiversity and soil biomass using soil DNA extraction and the tea bag index testing method. The filtered greywater absorbed by soil decomposed the soil faster and stabilized it better compared to tap water-absorbed soil or unfiltered greywater. The DNA generation sequencing revealed no significant differences in alpha diversity between control and treatment samples. The beta diversity differences were significant. This nature-based solution can lead to reduced load on the sewage system, resulting in less wastewater generation.

This study presents a detailed assessment on the impact of the Brazilian legal framework related to the access and benefit sharing provisions of the UN Convention on Biological Diversity. The research is based on a comprehensive dataset that was systematically collected over several years, directly from the official government bodies responsible for its implementation. The aim of the study is to contribute to a fact-based discussion on the effectiveness of national ABS laws, focusing on the Brazilian legal framework, first established in the year 2000 and revised in 2015, as a case example. This study balances the costs and benefits of the Brazilian ABS system and assesses the regulatory challenges it poses to individuals, companies, and institutions that perform research, share knowledge, develop, manufacture or market products derived from Brazilian biodiversity. The study indicates that, after over 22 years of operation, the regulatory challenges are still real and relevant, and that the significant volume of data collected from users on access, prior commercialization and shipment of genetic materials abroad was never systematically assessed by the agencies in charge. Besides, it shows that the costs incurred by the government in managing the policy itself have been higher than the economic benefits it has made possible, and that the institutional mechanisms in place since 2015 have not been able to allocate the monetary benefits contributed by users to the National Benefit Sharing Fund to any projects aimed at developing sustainable uses or preserving Brazilian biodiversity.

Work has begun in earnest to formulate a post-2020 Global Biodiversity Framework which will outline the vision and targets for the next decade of biodiversity conservation and beyond. However, the performance of the 2011-2020 Strategic Plan for Biodiversity suggests that even a meaningful target can fail to deliver if not accompanied by fit-for-purpose indicators. Here we provide a review of how ‘protected area’ effectiveness was addressed in the 2011-2020 plan and based on this, provide recommendations for fit-for-purpose indicators that will measure how such efforts contribute to the conservation of biodiversity. Indicators need to be built on quantitative data from site-level biodiversity monitoring of species and ecosystems combined with measurements of the state of nature in near-time, informed by remote-sensed products and other technologies. Additionally, indicators need to capture whether the essential elements of good management are in place including the identification of ecological values, threats, and objectives, equitable governance, and sufficient management resources and capacity. These fit-for-purpose indicators will require multilateral collaboration to galvanize support for, and resources to develop, the necessary infrastructure to collate and store information from countries.

The study was conducted in coniferous and deciduous old growth forests in two forest complexes located in: i) the fenced area of the Popielno Research Station of the Polish Academy of Sciences, with free-living Polish pony [Polish primitive horse (Equus ferus caballus)], and ii) open Maskulińskie Forest District managed (harvested) forest, without horses. The impact of forest animals on ground cover layer as well as on understory shrub layer and undergrowth, in i) area (horses and other forest animals) was compared with the results in ii) area (forest animals without horses). Very significant differences in the understory and undergrowth (above 0,5 m) layer vegetation communities structure between both areas and type of stands were found. The results suggest that the presence of the Polish horse substantially changed the species composition and increased the species diversity of the ground layer and shrub layer both on coniferous forest and in the deciduous forest habitats. The height of the shrub layer trees was lower by 30% in the area with the Polish horse.

As described in previous work, the use of synthetic chemical ingredients in modern cosmetics is postulated to be a cause of damage to the skin microbiome. The discovery that biodiversity on the human skin is currently the only reliable indicator of skin health, meant that for the first time, a mechanism to test for healthy skin was possible. Using this mechanism and in collaboration with The Medical University of Graz, who carried out the independent study, this work aimed to help answer whether modern day synthetic cosmetics are a main cause of long term damage to the skin microbiome. Thirty-two human participants tested three different face washes for their effect on the skin’s microbial diversity, along with skin pH, moisture and TEWL (trans-epidermal water loss), washing twice a day for four weeks. The upper volar forearm of the volunteers was swabbed at the beginning, two weeks in and end (four weeks). 16S rRNA sequencing was used. One leading ‘natural’ brand full of synthetic ingredients, a leading synthetic brand and a 100% natural face wash were used. Results give the first indications of a link between synthetic ingredients in a cosmetics product, and its effect on skin microbiome biodiversity. It paves the way for future studies on the topic with a larger sample group, longer test period and standardised methodology to create a universal standard for testing the health of skin using benchmark diversity values. This can be used in the future to test the effectiveness of cosmetics or ingredients on skin health, leading to the banning of products proven to harm the skin’s natural environment.

Background: Number of investigations, which are focused on arbuscular mycorrhiza fungi (AMF) biodiversity is still limited. The analysis of AMF taxa in the North Caucasus, a temperate biodiversity hotspot, used to be limited by the genera level. The aim of this study was to define AMF biodiversity at the species level in the North Caucasus biotopes. Methods: Molecular genetic identification of fungi was carried out with ITS1 and ITS2 regions as barcodes, sequencing by Illumina MiSeq, analysis of phylogenetic trees for individual genera, searching the operational taxonomic units (OTUs) with identification at the species level. Sequences from MaarjAM and NCBI GenBank were used as references. Results: We analyzed >10 million reads in soil samples for 3 biotopes to estimate fungal biodiversity. 50 AMF species from 20 genera were found. The valley biotopes (disturbed ecosystems, grasslands) were characterized by a greater AMF diversity, as well as the number of OTUs and species, in comparison with the forest biotopes. Conclusion: Our hypothesis is that high AMF biodiversity for the river valley can be associated with a higher percentage of annual plants in these biotopes, as well as with the active development of restorative successional processes.

Paenibacillus dendritiformis UJA2219 isolated from carrot produces broad-spectrum antibacterial activity. The aim of the present study was to determine the impact of partially-purified cell-culture extracts of strain UJA2219 on the microbial load and bacterial diversity of a home-made vegetable puree. The puree was challenged with an overnight culture of strain UJA2219 or with cultured broth extracts partially purified by cation exchange (CE) chromatog-raphy or reversed-phase (RP) chromatography and incubated for 7 days at temperatures of 4 °C or 25 °C. Best results were obtained at 25 °C with the RP extract, decreasing counts of presump-tive Enterobacteriaceae below detectable levels. The bacterial diversity of control and treated puree was studied by Illumina paired-end sequencing using DNA extracted from the puree samples incubated at 25 °C for 24 h. The controls and the puree inoculated with the UJA2219 strain showed an almost-identical bacterial diversity profile, with Proteobacteria (mainly Fam. Pseudo-monadaceae -gen. Pseudomonas- and Enterobacteriace as most abundant groups). Greatest differences in bacterial diversity were obtained in the puree treated with RP extract, showing a decrease in the relative abundance of Proteobacteria (especially gen. Pseudomonas) and an increase of Firmicutes (mainly of the genera Bacillus, Enterococcus and Lactococcus). Results from the study suggest that the antimicrobial preparations from strain UJA2219 have a potential for application in food bio-preservation.

The emergence of pathogenic fungi is a major and rapidly growing problem (7% increase) that affects human and animal health, ecosystems, food security and the economy worldwide. The Dermocystida group in particular has emerged relatively recently and includes species that affect both humans and animals. Within this group, one species in particular, Sphareothecum destruens, also known as the Rosette agent, represents a major risk to global aquatic biodiversity and aquaculture and has caused severe declines in wild fish populations in Europe and large losses in salmon farms in the USA. It is a species that has been associated with a healthy carrier for millions of years but the host has managed in recent decades to invade parts of Southeast Asia, Central Asia, Europe and North Africa. In order to better understand the emergence of this new disease, we have for the first time synthesised current knowledge on the distribution, detection, prevalence of S. destruens as well as the associated mortality curves, and the potential economic impact in countries where the healthy carrier has been introduced. Finally, we propose solutions and perspectives to manage and mitigate the emergence of this fungus in countries where it has been introduced.

Plans are currently being drafted for the next decade of action on biodiversity – both the post-2020 Global Biodiversity Framework of the Convention on Biological Diversity (CBD) and Biodiversity Strategy of the European Union (EU). Freshwater biodiversity is disproportionately threatened and under-prioritized relative to the marine and terrestrial biota, despite supporting a richness of species and ecosystems with their own intrinsic value and providing multiple essential ecosystem services. Future policies and strategies must have a greater focus on the unique ecology of freshwater life and its multiple threats, and now is a critical time to reflect on how this may be achieved. We identify priority topics including environmental flows, water quality, invasive species, integrated water resources management, strategic conservation planning, and emerging technologies for freshwater ecosystem monitoring. We synthesize these topics with decades of first-hand experience and recent literature into 14 special recommendations for global freshwater biodiversity conservation based on the successes and setbacks of European policy, management and research. Applying and following these recommendations will inform and enhance the ability of global and European post-2020 biodiversity agreements to halt and reverse the rapid global decline of freshwater biodiversity.

The Philippines is both a biodiversity hotspot and a megadiverse country. The country also has experienced one of the highest rates of deforestation in Southeast Asia and is among the first countries to introduce a massive reforestation program to address the country’s rapid biodiversity and forest loss. Drawing upon an empirical study from the Leyte island and other relevant case studies from the Philippines, in this chapter, we demonstrate that recovering secondary forests following shifting cultivation, locally known as kaingin have the high potentials for biodiversity and carbon co-benefits. Based on our empirical study, we also found that secondary forest regrowing after kaingin use can potentially be used as a cost-effective reforestation measure with multiple benefits to people and the environment in upland areas of the Philippines. We also discuss measures that are essential for such programs to be successful.

In recent years, Web APIs have become a de facto standard for exchanging machine-readable data on the Web. Despite this success though, they often fail in making resource descriptions interoperable due to the fact that they rely on proprietary vocabularies that lack formal semantics. The Linked Data principles similarly seek the massive publication of data on the Web, yet with the specific goal of ensuring semantic interoperability. Given their complementary goals, it is commonly admitted that cross-fertilization could stem from the automatic combination of Linked Data and Web APIs. Towards this goal, in this paper we leverage the micro-service architectural principles to define a SPARQL Micro-Service architecture, aimed at querying Web APIs using SPARQL. A SPARQL micro-service is a lightweight SPARQL endpoint that provides access to a small, resource-centric, virtual graph. In this context, we argue that full SPARQL Query expressiveness can be supported efficiently without jeopardizing servers availability. Furthermore, we demonstrate how this architecture can be used to dynamically assign dereferenceable URIs to Web API resources that do not have URIs beforehand, thus literally ``bringing'' Web APIs into the Web of Data. We believe that the emergence of an ecosystem of SPARQL micro-services published by independent providers would enable Linked Data-based applications to easily glean pieces of data from a wealth of distributed, scalable and reliable services. We describe a working prototype implementation and we finally illustrate the use of SPARQL micro-services in the context of two real-life use cases related to the biodiversity domain, developed in collaboration with the French National Museum of Natural History.

There is increasing evidence that the impact of climate change on the productivity of grasslands will at least partly depend on their biodiversity. A high level of biodiversity may confer stability to grassland ecosystems against environmental change, but there are also direct effects of biodiversity on the quantity and quality of grassland productivity. To explain the manifold interactions, and to predict future climatic responses, models may be used. However, models designed for studying the interaction between biodiversity and productivity tend to be structurally different from models for studying the effects of climatic impacts. Here we review the literature on the impacts of climate change on biodiversity and productivity of grasslands. We first discuss the availability of data for model development. Then we analyse strengths and weaknesses of three types of model: ecological, process-based and integrated. We discuss the merits of this model diversity and the scope for merging different model types.

Gastrotricha are microscopic benthic animals found in almost all water bodies. To date, about 870 species distributed in 71 genera, 18 families, and two orders are known. Known freshwater species are 359, several of which found also in Italy; however, a compendium of the studies carried out so far and a georeferenced distribution of the species still needs to be provided. This project aims to summarize information about Italian gastrotrichs' diversity and geographic distribution. Diversity data, acquired over 239 years, were reviewed and corrected based on taxonomic and nomenclatural updates and, in addition to distribution information, were organized into a data matrix useful for statistical analysis and to be feed to a Geographic Information System software to easily understand the overall figures. Results indicate Italy as one best-known country regarding freshwater gastrotrich, with 88 species in 16 genera and 3 families from 59 investigated localities; the Nation counts 16 type localities and 19 endemic species. Despite its censed biodiversity, many Italian regions remain still poorly or not investigated where future research should focus. Implementing a web mapping plugin enabled the creation of interactive maps for an easy and modern sharing of the work done and information acquired.

Centropomus undecimalis (common snook, sergeant fish or robalo) and Centropomus parallelus (fat snook, smallscale fat snook, little snook or chucumite) have tropical and subtropical wide distribution from southern Florida (Gulf of Mexico) to southern Brazil (Rio Grande do Sul) waters, and due the value of their flesh, these species have been explored with predatory fishing, generating a deficiency for their conservation. Adequate conservation of this species depends on population genetic parameters. Thus, in this study, we used microsatellite markers to investigate the genetic variability and the genetic characterization of C. undecimalis and C. parallelus populations, identifying if the citations species are geographically connected, using 10 loci of microsatellites, bringing strategies of management, sustainability, and conservation for species citations, and providing genetic subsidies needed to be used in breeding programs for snooks. Microsatellite molecular genetic markers were efficient in the study of the genetic variability of the samples of C. parallelus and C. undecimalis, resulting in data on the genetic structuring of these populations, thus aiding in their management and conservation. The data presented may provide subsidies for future use in programs aimed at animal breeding of sea bass, establishing tools for use in fish farming. In addition, despite being two species that are not on the endangered list, we can observe that there are genetic differences between the studied populations of the same species.

eDNA extracted from the gut contents of filter feeders with unique feeding habits can be used to identify biodiversity in aquatic ecosystems. In this study, we used eDNA from the gut contents of the clam Corbicula fluminea to examine estuarine ecosystem biodiversity. The field survey was conducted at three study sites in the Nakdong River Estuary, which is characterised by closed estuarine features resulting from the presence of an estuarine barrage. The collected C. fluminea samples were dissected to separate the gut contents, and the extracted eDNA was amplified using the 18S V9 primer targeting all eukaryotes. The amplified DNA was sequenced using next generation sequencing (NGS) techniques, and BLASTn was performed based on the NCBI database. We obtained 21 unique operational taxonomic units (OTUs), including fish (approximately 9.52%), copepods (approximately 14.29%), and green algae (approximately 23.81%), which represented a wide range of habitats such as marine, brackish, freshwater, and terrestrial environments. These results suggest that various organisms living in aquatic ecosystems can be identified through eDNA from the gut contents of C. fluminea and support the potential application of bivalves as eDNA samplers in diverse aquatic ecosystems.

Globally, sacred groves represent a traditional form of community-based conservation system, recognized for their capacity to preserve areas that are of cultural and religious importance to local people. In most cases, the entire community takes on a watchdog role to guard against encroachment and unauthorized access either by its members or outsiders who might desecrate such sites. Our paper investigates the effects of different governance arrangements on three sacred groves in southwest Nigeria⎯Osun Osogbo Sacred Grove (UNESCO World Heritage Site); Idanre Hills (Nigerian National Monument) and Igbo-Olodumare (local cultural site)⎯on their socio-economic and religio-cultural benefits and contribution to biodiversity conservation. Using a mixed-methods design of a semi-structured questionnaire (n=167), key informant interviews (n=2), and focus groups (n=7), we collected data from local community members, traditional priests, sacred grove devotees and tourism officials. We found that customary institutions have guided reverence for sacralized spaces and wise utilization of their unique resources. The growing recognition of sacred groves has paved the way for socioeconomic rewards for individuals and government as cultural tourism increases. We found that the involvement of formal institutions alongside customary institutions in sacred grove management reinforces compliance with conservation laws within the sacred groves, especially where traditional norms are weak or may be disregarded. We discuss the implications of these observations and offer suggestions to improve community engagement, uphold traditional ecological knowledge, and develop ecotourism within the groves. We conclude that the co-existence of community-based conservation through a system of established traditional norms and prohibitions as well as formal government legislation and management, offers assurance for the long-term preservation of sacred groves and their biodiversity.

Indonesia is one of the most biodiverse countries in the world and a promising resource for novel natural compound producers. Actinomycetes produce about two-thirds of all clinically used antibiotics. Thus, exploiting Indonesia’s microbial diversity for actinomycetes may lead to the discovery of novel antibiotics. A total of 422 actinomycete strains were isolated from three different unique areas in Indonesia and tested for their antimicrobial activity. Nine potent bioactive strains were prioritized for further drug screening approaches. The nine strains were cultivated in different solid and liquid media and a combination of genome mining analysis and mass spectrometry (MS)-based molecular networking was employed to identify potential novel compounds. By correlating secondary metabolite gene cluster data with MS-based molecular networking results, we identified several gene cluster-encoded biosynthetic products from the nine strains, including naphthyridinomycin, amicetin, echinomycin, tirandamycin, antimycin, and desferrioxamine B. Besides, eight putative ion clusters and numerous gene clusters were detected that could not be associated with any known compound, indicating that the strains can produce novel secondary metabolites. Our results demonstrate that sampling of actinomycetes from unique and biodiversity-rich habitats, such as Indonesia, along with a combination of gene cluster networking and molecular networking approaches, accelerates natural product identification.

A catastrophic loss of microbial biodiversity on the skin has led to alarming increase in the prevalence of allergies and long-term damage to the skin, which could also have damaging knock on effects to overall health. This study uses 50 human participants, to obtain an average (benchmark) value for the biodiversity of ‘healthy’ western skin, which is crucial in updating our 2017 skin health measuring mechanism to use standardised methodology. Previous work with a larger sample size was unsatisfactory for use as a benchmark due to its use of different and outdated diversity indices. We also investigated the effect of age and sex, two known skin microbiome affecting factors. Although no statistical significance is seen for age- and sex- related changes in diversity, there appear to be changes related to age which elaborates on previous work which used larger, more general age ranges. Our study indicates adults age 28-37 have highest diversity, and age 48-57 the lowest. Crucially, because of this study we are now able to update the skin health measuring mechanism from our 2017 work. This will aid diagnostic assessment of susceptibility to cutaneous conditions or diseases, and treatment. Testing any human subject will be rapidly improved by obtaining future benchmark diversity values for any age, sex, body site and area of residence, to which they can be compared. This improvement means we can also more accurately investigate the ultimate question: What factors in the western world are a main cause of the skin allergy epidemic? This could lead to future restriction of certain synthetic chemicals or products found to be particularly harmful to the skin.

Following the adoption of the Kunming-Montreal Global Biodiversity Framework (KM-GBF), with the Convention on Biological Diversity serving as its guiding treaty, the European Union (EU) has just reached an agreement on an EU Nature Restoration Regulation. This study carries out a systematic cross-walk between the restoration regulation and the KM-GBF, focusing on their implications for forest ecosystems. This paper identifies areas of alignment, divergence, and potential gaps related to habitat restoration, species protection, climate resilience, and the sustainable use of natural resources. The methodology adopts a grounded approach, starting with the 23 targets outlined in the KM-GBF and proceeding to the 28 articles set out in the restoration regulation. The results highlight the need for better alignment between the KM-GBF, the restoration regulation, and other forest-relevant EU policy instruments. The study stresses the need for a coherent and integrated EU policy approach that can address the diverse challenges and policy objectives facing forests. It concludes that amendments to the restoration regulation have significantly diluted its potential impact, limiting the EU Members States accountability and ability to meet KM-GBF goals and targets. It further stresses the need for strategies that can reconcile divergent EU policy pathways, support forest management and restoration efforts, while aligning with global biodiversity objectives.

The Mexican Republic ranks fifth in the world in terms of amphibian diversity, and within Mexico, the state of Oaxaca has the greatest amphibian richness and endemism. Unfortunately, various factors, such as land use change and global warming, have caused a global crisis that threatens the conservation of this class. In the state of Oaxaca, few studies of amphibians have been carried out at the state level. Several research has shown that an analysis of beta diversity provides information that can be applied to conservation strategies. In this work, we analyzed the beta diversity at the species level and higher taxon level for the amphibians of Oaxaca and their replacement components and the differences in richness for anurans and caudates separately between physiographic subprovinces. Very high beta diversity was recorded, with higher diversity occurring among caudates than among anurans. Replacement was the component that most contributed to this result, and the subprovinces with substantial environmental differences had the most dissimilar amphibian communities. The results of this study show the need to implement conservation strategies in subprovinces with high amphibian richness and endemism levels, following the example of Sierra Madre de Oaxaca (SMO)

Numerous studies have shown the multiple benefits of plant biodiversity and the crucial role of resident’s awareness in conservation and land management. Plant biodiversity is investigated in an analytical monitoring report conducted jointly with local population engaged in livestock activities and the young residents who are shifting toward hospitality and tourism. The study area, covering over 800 km2 in Sardinia, is half forested and has landscape interest. During the research, genotypes of Quercus ilex L. with sweet acorns and richer in polyphenols were discovered, the oldest ecosystem services in those rural communities. Collaborative monitoring focused attention on the benefits of plants in different habitats: 53% of these have been known to livestock farmers as food for local breeds of goats and pigs; 15% are officinal and 13% endemic. They have also been used in human nutrition within the Mediterranean diet, and they have landscape and garden interest. The study analyzes numerical data from a critical and educational perspective. These data serve as indicators of ecosystem health for the purpose of sustainable management policies, and attest to collaborative monitoring as a tool for analyzing human activities and the necessary balance between profit and biodiversity conservation, given the current challenging climate change conditions.

The genetic potentials of rice cultivars will need to be expressed to their fullest if global rice production is to be expanded enough by 2050 to meet the increased demand of expanding population while the availability of land and water per capita dwindles. New and ‘improved’ rice varieties have contributed greatly to increased production over the past 50 years, but the rate of rice yield increase based on genetic changes has declined in recent decades compared with the early years of the Green Revolution. In fact, many rice consumers continue to prefer to consume ‘traditional’ rice varieties (referred to also as local, native, unimproved, or indigenous) because of their taste, aroma, texture, and other qualities. Further, many farmers prefer to cultivate these varieties because of their better adaptation to local climatic and soil conditions and their evolved resistance to endemic stresses. The practices that comprise the System of Rice Intensification (SRI), including transplanting rice seedlings at a young age, wide spacing between plants, keeping the soil well-aerated rather than inundated, and enhancing soil organic matter, provide traditional rice varieties with micro-environments that are more favorable for the expression of their genetic and agronomic potentials. Interactions among rice plants, soil characteristics, water, energy, and other inputs improve the phenotypic and physiological performance of rice plants. This paper considers how the cultivation of traditional rice varieties with SRI methods can raise yields, reduce farmers’ costs of production, and generate higher incomes, while contributing to the conservation of rice biodiversity.

In 2008, Ecuador recognized the Constitutional Rights of Nature in a global first. This recognition implies a major shift in the human-nature relationship, from one between a subject with agency (humans) and an exploitable object (Nature), to a more equilibrated relationship of respect. However, the lack of a standard legal framework has left room for subjective interpretations and variable implementation. The recent widespread concessioning of pristine ecosystems to mining industries in Ecuador has set up an unprecedented conflict and test of these rights. Currently, a landmark case involving Los Cedros Protected Forest and mining companies has reached the Constitutional Court of Ecuador. If Ecuador’s highest Court rules in favor of Los Cedros and the Rights of Nature, it would set a legal precedent with enormous impact on biological conservation in Ecuador and, potentially, the world. Such a policy shift offers a novel conservation strategy, through citizen oversight and action.

Tropical island countries are often highly populated and deliver immense ecosystem service benefits. As human wellbeing depends on these ecosystems proper management is crucial in the resource-rich tropical lands where related research is less. Though the ecosystem service and biodiversity studies are a promising path to inform the ecosystem management for these mostly developing countries published evidence of using ecosystem service studies in decision-making is lacking. The purpose of this study is to provide an overview of ecosystem services and related research in Sri Lanka examining trends and gaps and how these studies are conceptualized. Out of considered 139 peer-reviewed articles majority of articles 42.4% were terrestrial and forest related while coastal ecosystems were considered in 34.5% of studies. In most studies, the ecosystem service category was provisioning (33.8%) followed by regulatory service (30.9%). Studies investigating and quantifying ecosystem services, pressures on ecosystems, and their management were fewer compared to studies related to biodiversity or species introduction. Moreover, studies investigating the value of ecosystem services and biodiversity to the communities or involvement of stakeholders in the development of management actions regarding the ecosystem services were rare in Sri Lanka and intense focus of future studies in these aspects are timely and necessary.

Due to rising global warming and climate change, biodiversity protection has become a critical ecological concern. The rich biodiversity zones are under threat and are deteriorating, necessitating national, regional, and provincial efforts to safeguard these natural areas. The effective conservation of National Parks and Nature-protected Areas helps to improve biodiversity conservation, forest, and urban air quality. The continuous encroachment and abuse of these protected areas have degraded the ecosystem over time. While exploring the geophysical ecology and biodiversity conservation of these areas in West Africa, Kainji National Park was selected for this study because of its notable location, naturalness, rich habitat diversity, topographic uniqueness, and landmass. The conservation of national parks and nature-protected areas is a cornerstone of biodiversity conservation globally. This study is aimed at the target of United Nations Sustainable Development Goal 13, 2030- Climate Action, targeted at taking urgent action towards combating climate change and its impacts. The study captures both flora and fauna that are dominant in the study area. The 15 identified tree species were selected from over 30 species with 563,500,000 (an average of 3,700,000 in each sample frame) trees for every tree species/type with a total of 63% tree green canopy cover. The study areas divided into three zones were randomly sampled within a stratum of 25x25km frames divided into 150 sample frames for proper analyses using the i-Tree Eco v6.0.23. The following microclimatic data were captured and analyzed; photosynthetically active radiation, rain/precipitation, temperature, transpiration, evaporation, water intercepted by trees, avoided a runoff by trees, potential evaporation by trees, isoprene and monoterpene by trees. This study also further discusses the tree benefits of a green, low carbon, and sustainable environment within the context of biodiversity conservation considering carbon storage, carbon sequestration, hydrology effects, pollution removal, oxygen production, and volatile organic compounds (VOCs). There is a quick need for remotely-sensed information about the protected areas at regular intervals and government policies must be strict against illegal poaching, logging activities and other hazardous human impacts.

Among non-conventional yeasts of industrial interest, the dimorphic oleaginous yeast Yarrowia lipolytica appears as one of the most attractive for a large range of white biotechnology applications, from heterologous proteins secretion to cell factories process development. The past, present and potential applications of wild type, traditionally improved or genetically modified Yarrowia lipolytica strains will be resumed, together with the wide array of molecular tools now available to genetically engineer and metabolically remodel this yeast. The present review will also provide a detailed description of Yarrowia lipolytica strains and highlight the natural biodiversity of this yeast, a subject little touched upon in most previous reviews. This work intends to fill this gap by retracing the genealogy of the main Yarrowia lipolytica strains of industrial interest, by illustrating the search for new genetic backgrounds and by providing data about the main publicly available strains in yeast collections worldwide. At last, it will focus on exemplifying how advances in engineering tools can leverage a better biotechnological exploitation of the natural biodiversity of Yarrowia lipolytica and of other yeasts from the Yarrowia clade.

A study was carried out in a Waste Sorting Plant (WSP) located in France and treating dry recyclable household waste (DRHW) as well as dry recyclable commercial and industrial waste (DRCIW). The aim was to investigate the exposure to bioaerosol and dust. The aim of the study was to assess the extent to which the measurement of concentration, species composition and particle size distribution can contribute to a better assessment of the biological risks associated with exposure. Both stationary and personal bioaerosol and dust samples were collect in the (WSP). The results confirm that waste and waste sorting activities are sources of airborne fungi. Indeed, ambient concentrations ranged from 7.3×103 to 8.5×105 CFU/m3 for culturable moulds and up to up to 4 mg/m3 for dust. Personal exposure to inhalable dust was found up to 3 mg/m3 for dust and ranged from 8.6×103 to 1.5×106 CFU/m3 for fungi. Airborne fungal communities were found to be dominated by the Penicillium genera in both bioaerosols and settled dust samples, followed by the Aspergillus, Cladosporium, Wallemia, Mucor, and Rhizopus genera. Fungi were carried by particles of aerodynamic diameters mainly between around 2.0 and 10.0 µm. The findings dealing with size distribution and biodiversity of bioaerosols suggest that employees are exposed to complex bioaerosols during their work and helps to make a finer diagnosis of the risks involved, which is often difficult in the absence of any OEL for bioaerosols in general.

The spectacular radiation of insects has produced a stunning diversity of phenotypes. During the last 250 years, research on insect systematics has generated hundreds of terms for naming and comparing those phenotypes. In its current form, this terminological diversity is presented in natural language and lacks formalization, which prohibits computer-assisted comparison using semantic web technologies. Here we propose a Model for Describing Insect Anatomical Structures (MoDIAS) which incorporates structural properties and positional relationships for standardized, consistent, and reproducible descriptions of insect phenotypes. We applied the MoDIAS framework in creating the ontology for the Anatomy of the Insect Skeleto-Muscular system (AISM). The AISM is the first general insect ontology that aims to cover all taxa by providing generalized, fully logical, and queryable, definitions for each term. It was built using the Ontology Development Kit (ODK), which maximizes interoperability with Uberon (Uberon multi-species anatomy ontology) and other basic ontologies, enhancing the integration of insect anatomy into the broader biological sciences. A template system for adding new terms, extending and linking the AISM to additional anatomical, phenotypic, genetic, and chemical ontologies is also introduced. The AISM is proposed as the backbone for taxon-specific insect ontologies and has potential applications spanning systematic biology and biodiversity informatics, allowing users to (1) use controlled vocabularies and create semi-automated computer-parsable insect morphological descriptions; (2) integrate insect morphology into broader fields of research, including ontology-informed phylogenetic methods, logical homology hypothesis testing, evo-devo studies, and genotype to phenotype mapping; and (3) automate the extraction of morphological data from the literature, enabling the generation of large-scale phenomic data, by facilitating the production and testing of informatic tools able to extract, link, annotate, and process morphological data. This system will allow for clear and semantically interoperable integration of insect phenotypes in biodiversity studies.

Buruli ulcer (BU), caused by Mycobacterium ulcerans, is a neglected tropical disease associated with freshwater habitats. A variety of limnic organisms harbor this pathogen, including aquatic bugs (Hemiptera: Heteroptera), which have been hypothesized to be epidemiologically important reservoirs. Aquatic Hemiptera exhibit high levels of diversity in the tropics, but species identification remains challenging. In this study, we collected aquatic bugs from emerging foci of BU in the Southwest Region of Cameroon, which were identified using morphological and molecular methods. The bugs were screened for mycobacterial DNA and a selection of 20 mycobacteria-positive specimens from the families Gerridae and Veliidae were subjected to next-generation sequencing. Only one individual revealed putative M. ulcerans DNA, but all specimens contained sequences from the widespread alpha-proteobacterial symbiont, Wolbachia. Phylogenetic analysis placed the Wolbachia sequences into supergroups A, B and F. Circularized mitogenomes were obtained for seven gerrids and two veliids, the first from these families for the African continent. This study suggests that aquatic Hemiptera may have a minor role (if any) in the spread of BU in Southwest Cameroon. Our metagenomic analysis provides new insights into the incursion of Wolbachia into aquatic environments and generates valuable resources to aid molecular taxonomic studies of aquatic Hemiptera.

The altitude of the habitat is one of the important regulators of species survival. Kaçkar Mountains National Park is located in the Eastern Black Sea region of Turkey. This is the first study on the benthic diatom flora of the high mountain lakes in Kaçkar Mountains National Park, which is situated between 2782 and 3075 m a.s.l. A total of 84 diatom species were identified from benthic communities of 15 habitats in summer (19 July, 28 August) and autumn (10 September) months of 2020. The genus Pinnularia (13 species) formed the basis of the taxonomic list followed by Eunotia (5 species), Navicula (5 species), and Frustulia (4 species) genera, respectively. The waters in all the studied lakes were fresh, low-saline, with low-alkaline or circumneutral pH and organically uncontaminated as evidenced by prevailed bioindicator groups. Statistical methods and comparative floristic results confirm the role of the lake altitude for the diatom species distribution. Species richness was higher in lakes with lower altitude, but a statistical approach also revealed a tendency for it to increase with altitude if species richness increased. Further studies will be needed to continue exploring this pattern. In the first round of analysis, diatom species groups were categorized according to IUCN categories, revealing one potentially threatened and one rare species. To protect studied high mountain lakes, their ecological conditions must be constantly monitored in the Kaçkar Mountains National Park.

Multispectral and 3D LiDAR remote sensing data sources are valuable tools for characterizing the 3D vegetation structure and thus understanding the relationship between forest structure, biodiversity and microclimate. This study focuses on mapping riparian forest species in the canopy strata using a fusion of Airborne LiDAR data and multispectral multi-sources and multi-resolution satellite imagery; Sentinel-2 and Pleiades at tree level. The idea is to assess the contribution of each data source in the tree species classification at the considered level. The data fusion was processed at feature-level and decision level. At feature level, LiDAR 2D attributes were derived and combined with vegetation indices. At decision level, LiDAR data was used for 3D tree crown delimitation providing unique trees or groups of trees that are used as a support for the species classification. Data augmentation techniques were used to improve the training process. Best results were obtained by the fusion of Sentinel-2 time series and LiDAR data with a Kappa of 0.66 thanks to red-edge based indices that better discriminate vegetation species and the temporal resolution of Sentinel-2 images that allows monitoring the phenological stages helping to discriminate the species.

Public access to high quality green environments has become a key issue for city managers and a matter of environmental justice. Remnants of natural ecosystems allow citizens a direct contact with nature, but conversely the presence of people contributes further to the existing disturbances. Urban pressures on ecosystem remnants may act to favour the expansion of some invasive species in cities. Whilst the negative impacts of invasive species on ecosystem function is well documented little is known how invasive species influence the use of green spaces by people. Here, we examined one of the few remnants of urban riparian forests in Europe, the Vistula river valley in Warsaw which has recently become an attractive recreation site. Despite their high ecological value, the poplar and willow forests have been increasingly taken over by the invasive tree species Acer negundo. We examined the status of the invasion process and the relationship between recreational ecosystem services and the characteristics of the tree stands – tree species, tree density and age and NDVI values. We found the willow forest to be more susceptible to invasion by A. negundo than the poplar forest, which was revealed in significantly higher share of the maple individuals and their greater volume per unit area. Presence of A. negundo affected biodiversity resulting in decreased undergrowth density and number of species. The use intensity by the public, assessed on the basis of trampling intensity and the density of existing informal tracks, were negatively correlated to the presence of A. negundo. This study highlights the need to integrate invasive species management into green infrastructure planning and management.

The influenza virus has been the primary cause of the pandemic, posing a constant threat to human society. Due to its genetic evolution and continuous outbreak, antiviral research currently focuses on exploring a novel lead agent. A comprehensive antiviral screening discovered a marine bacterium whose extract exerted excellent efficacy against influenza viruses. Parerythrobacter sp. M20A3S10, a novel strain under the family Erythrobacteraceae, could produce carotenoids exhibiting antiviral and anticancer activity by enhancing the cellular immune system. Post-treatment of M20A3S10 extract showed outstanding therapeutic indexes: against influenza virus A/PR8 (H1N1) [selectivity index (SI) = 24.0], A/Wisconsin/15/2009 (H3N2) (SI = 30.1) and B/Florida/78/2015 (SI = 38.2). Comparably, the effectiveness was demonstrated against Zika virus (ZIKV) and dengue virus type 2 (DENV2) with an SI of 22.5 and 24.1, respectively, namely broad-spectrum activity. Of note, the antiviral responses resulted from the common replication mechanism between IAV, ZIKV, and DENV2. The stimulation of apoptosis-mediated cellular immunity prevented the viral release and protected the host, suggesting that switching from necroptosis to apoptosis is a novel antiviral target. Although the specific compound affecting the antiviral activity was not identified, its promising efficacy with broad activity will contribute to developing a strategy for preventing future pandemics.

The outstanding occurrences of red wood ant nests (RWA; Formica rufa-group) at two study sites in the Oberpfalz, NE Bavaria, Germany, could be confirmed by the 2023 re-inventory (a total of 5,393 nests and an increase by ≈8 %). RWA nests preferred mature and medium mature pine-dominated forests. Standing and downed dead wood do not negatively influence nests. Nest counts with woodpecker cavities and the diversity of the herb layer increased. The highly clustered nests in NW-SE direction suggests a strong interaction between nests distributions and the tectonic environment. Our developed (re-)inventory approach in combination with the comprehensive photo database is a valuable tool for a) monitoring the entire forest habitat including woodpecker cavities and dead wood in, at and around nests, and b) identifying GeoBio-Interactions. Such a comprehensive approach leads to more accurate and realistic counts of RWA nests during (re-)inventories and provides scientific evidence of the status quo of RWA occurrence. Our results disproved the unfounded and scientifically unsubstantiated blanket statement by laymen of the ASW Landesverband Bayern e.V. that RWA nest numbers in the Oberpfalz or even in the entire Bavaria are in sharp decline. We suggest seven to eight years as a suitable time interval for re-inventories.

The opportunities and potential of Artificial Intelligence (AI) in Environmental Assessment (EA) are often mentioned. However, do we in the EA field understand the implications of what is happening in other biological sciences, and are we preparing for the changes that are coming? This interdisciplinary letter focuses on AI-driven developments in biodiversity data and analysis as a starting point for stimulating discussion about what AI means in practice for the field of EA. We highlight implications for training, transformation of practice and decision making as first steps in a research agenda.

Hazardous toxic metals, such as lead and cadmium, and to a lesser extent aluminum, are extensively recognized as detrimental for health following ingestion within food and water, or following inhalation. Gut and food-derived microbes, by interacting with heavy metals, may actively or passively modulate their bioavailability inside the gut, either by adsorption or by sequestration. Such a bioremediation within the gut implies the selection of safe microbes, based on their specific capacities to immobilize metals. We investigated the metal removal ability of 225 bacteria toward the potential harmful trace elements lead, cadmium and aluminum in vitro, using Inductively Coupled Plasma Mass Spectrometry analysis. Interspecies and intraspecies comparisons were addressed and discussed among bacteria from the phylum Firmicutes, which are mostly lactic acid bacteria, including Lactobacillus spp, with some Lactococcus, Pediococcus and Carnobacterium representatives, Actinobacteria as well as Proteobacteria. The effect on mixture of lead and cadmium was also investigated. Although the purpose of such a screening is so far not to elucidate each of the various strain specific- and metal dependent- mechanisms of heavy metal removal, we identified potential bacteria which are able to alleviate Pb(II) and Cd(II) concerns in order to propose performing candidate probiotics for metal xenobiotic bioremediation.

The application of smartphone-based color evaluation of passive sampling devices for gases has only been sparsely reported. The present review aims to compile available publications with respect to the configuration of the passive samplers, conditions of smartphone photographing, analytical procedure for color detection and quantification (including calibration processes), and their application to different target gases. The performance of the methods - whenever available - is presented regarding the analytical specifications selectivity, sensitivity, and limit of detection in comparison with other color evaluation methods of passive samplers. Practical aspects like requirements of instrumentation and ease of use will be outlined in view of the potential employment in education and citizen science projects. In one section of the review the inconsistent terminology of passive and diffusive sampling is discussed in order to clarify the distinction of information obtained from the uptake of the passive samplers between gas-phase concentration and the accumulated deposition flux of gaseous analytes. Colorimetric gas sensors are included in the review when applied in passive sampling configurations and evaluation is performed with smartphone-based color evaluation. Differences in the analytical procedures employed after the passive sampling step and prior to the detection of the colored compounds are also presented.

Epidemic diseases that arise from infectious RNA viruses, particularly influenza viruses, pose a constant threat to the global economy and public health. Viral evolution has undermined the efficacy of acquired immunity from vaccines and the antiviral effects of FDA-approved drugs. As such, there is an urgent need to develop new antiviral lead agents. Natural compounds, owing to their historical validation of application and safety, have become a promising solution. In this light, a novel marine bacterium, Pseudomonas sp. M20A4R8, has been found to exhibit significant antiviral activity [half maximal inhibitory concentration (IC50) = 1.3 µg/mL, selectivity index (SI) = 919.4] against influenza virus A/Puerto Rico/8/34, surpassing the activity of chloroquine. The antiviral response by M20A4R8 extract was induced during post-entry stages of the influenza virus, indicating suitability for post-application after the establishment of viral infection. Furthermore, post-treatment with M20A4R8 extract protected the host from virus-induced apoptosis, suggesting its potential use in acute respiratory disease complexes resulting from immune effectors' overstimulation and autophagy-mediated self-apoptosis. The extract demonstrated an outstanding therapeutic index against influenza virus A/Wisconsin/15/2009 (IC50 = 8.1 µg/mL, SI = 146.2) and B/Florida/78/2015 Victoria lineage (IC50 = 3.5 µg/mL, SI = 343.8), indicating a broad anti-influenza virus activity with guaranteed safety and effectiveness. This study provides a new perspective on mechanisms for preventing a broad spectrum of viral infections through antiviral agents from novel and natural origins. Future studies on a single or combined compound from the extract hold promise, encouraging its use in preclinical challenge tests with various influenza virus strains.

Ethical considerations regarding our treatment of animals have gained strength, leading to legislation and a societal focus across various disciplines. This is a subject of study within curricula related to agri-food sciences. The aim was to determine the perceptions of agronomy university students concerning animal welfare in livestock production systems. A survey was conducted to encompass various aspects, from participants' sociodemographic attributes to their attitudes and behaviors regarding animal welfare and the consumption of animal products. Statistical analysis, performed using R software, delved into the associations between participants' characteristics and their perspectives on the ethical, bioethical, and legal dimensions of animal welfare. Associations between demographic factors and ethical viewpoints among students were identified. Gender differences emerged in animal treatment perceptions, while rural and urban environments impacted perspectives on various animals. Bioethical considerations revealed distinctive disparities based on gender and education in concerns for animal welfare, value perceptions, evaluations of animal behaviors, and opinions on animal research. It is crucial to distinguish between animal welfare and ethical considerations arising from coexisting with sentient beings capable of experiencing suffering. Ethical theories provide a lens through which we perceive our obligations toward animals. The responsibility to ensure animal welfare is firmly rooted in recognizing that animals, like humans, experience pain and physical suffering. Consequently, actions causing unjustified suffering or mistreatment, particularly for entertainment purposes, are considered morally unacceptable.