Epigallocatechin Gallate Affects the Structure of Chromatosomes, Nucleosomes and Their Complexes with PARP1

Tatiana V. Andreeva; Natalya V. Maluchenko; Anastasiya V. Efremenko; Alexander V. Lyubitelev; Ann N. Korovina; Dmitry A. Afonin; Mikhail P. Kirpichnikov; Vasily M. Studitsky; Alexey V. Feofanov

doi:10.20944/preprints202308.1874.v1

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preprints.org > biology and life sciences > biophysics > doi: 10.20944/preprints202308.1874.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Epigallocatechin Gallate Affects the Structure of Chromatosomes, Nucleosomes and Their Complexes with PARP1

Tatiana V. Andreeva

Natalya V. Maluchenko

Anastasiya V. Efremenko

Alexander V. Lyubitelev

Ann N. Korovina

Dmitry A. Afonin

Mikhail P. Kirpichnikov

Vasily M. Studitsky

^* ,

Alexey V. Feofanov

Version 1 : Received: 25 August 2023 / Approved: 28 August 2023 / Online: 29 August 2023 (03:03:39 CEST)

A peer-reviewed article of this Preprint also exists.

Andreeva, T.V.; Maluchenko, N.V.; Efremenko, A.V.; Lyubitelev, A.V.; Korovina, A.N.; Afonin, D.A.; Kirpichnikov, M.P.; Studitsky, V.M.; Feofanov, A.V. Epigallocatechin Gallate Affects the Structure of Chromatosomes, Nucleosomes and Their Complexes with PARP1. Int. J. Mol. Sci. 2023, 24, 14187. Andreeva, T.V.; Maluchenko, N.V.; Efremenko, A.V.; Lyubitelev, A.V.; Korovina, A.N.; Afonin, D.A.; Kirpichnikov, M.P.; Studitsky, V.M.; Feofanov, A.V. Epigallocatechin Gallate Affects the Structure of Chromatosomes, Nucleosomes and Their Complexes with PARP1. Int. J. Mol. Sci. 2023, 24, 14187.

Abstract

The natural flavonoid epigallocatechin gallate (EGCG) has a wide range of biological activities including binding to nucleic acids; however, mechanisms of interaction of EGCG with DNA organized in chromatin have not been systematically studied. In this work, interaction of EGCG with chromatin in cells, and with nucleosomes and chromatosomes in vitro was studied using fluorescent microscopy and single particle Förster resonance energy transfer approaches, respectively. EGCG effectively penetrates into the nuclei of living cells and binds to DNA there. Interaction of EGCG with nucleosomes in vitro induces large-scale, reversible uncoiling of nucleosomal DNA that occurs without dissociation of DNA or core histones at sub- and low-micromolar concentrations of EGCG. EGCG does not reduce the catalytic activity of poly(ADP-ribose) polymerase 1 (PARP1), but causes modulation of the structure of the PARP1-nucleosome complex. EGCG significantly changes the structure of chromatosomes, but does not cause dissociation of the linker histone. Reorganization of nucleosomes and chromatosomes by EGCG might facilitate access of protein factors involved in DNA repair, replication and transcription to DNA and thus might contribute to the modulation of gene expression by EGCG that was reported earlier.

Keywords

epigallocatechin gallate; nucleosome; chromatosome; poly(ADP-ribose) polymerase 1; spFRET microscopy

Subject

Biology and Life Sciences, Biophysics

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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