Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Antimicrobial Activity of the Essential Oil from Croton Pluriglandulosus Carn. Leaves against Microorganisms of Clinical Interest

Version 1 : Received: 9 May 2023 / Approved: 9 May 2023 / Online: 9 May 2023 (08:46:49 CEST)

A peer-reviewed article of this Preprint also exists.

Carvalho, R.J.P.; Souza, P.F.N.; Malveira, E.A.; Neto, N.A.S.; Silva, R.R.S.; Melo, G.L.C.; Silva, A.F.B.; Lima, L.B.; de Albuquerque, C.C.; Bastos, R.W.; Goldman, G.H.; de Freitas, C.D.T. Antimicrobial Activity the Essential Oil from Croton pluriglandulosus Carn. Leaves against Microorganisms of Clinical Interest. J. Fungi 2023, 9, 756. Carvalho, R.J.P.; Souza, P.F.N.; Malveira, E.A.; Neto, N.A.S.; Silva, R.R.S.; Melo, G.L.C.; Silva, A.F.B.; Lima, L.B.; de Albuquerque, C.C.; Bastos, R.W.; Goldman, G.H.; de Freitas, C.D.T. Antimicrobial Activity the Essential Oil from Croton pluriglandulosus Carn. Leaves against Microorganisms of Clinical Interest. J. Fungi 2023, 9, 756.

Abstract

Multiresistant pathogens pose a serious threat to human health. The genus candida is one class of human pathogenic yeasts responsible for infections affecting healthy and immunocompromised patients. In this context, plant essential oils emerged as a future natural alternative to control the diseases caused by these pathogens. Here, it highlighted antimicrobial activity and the mechanisms of action of the essential oil extracted from Croton pluriglandulosus Carn.-Torres & Riina (CpEO) leaves on human pathogenic microorganisms in planktonic and biofilm lifestyles. In addition, for the first time, the oil composition was revealed by GC-MS analysis and the toxicity to human red blood cells (HRBC). Twenty-six chemical compounds were identified in EOCp, Elemicin, Bicyclogermacrene, caryophyllene, brevifolin and 2,4,6-trimethoxy-styrene. Through hemolytic assay, it was shown that CpEO has no toxicity to human RBCs. At the concentration of 50 μg mL-1, CpEO did not show great antibacterial potential. However, promising data were found for C. krusei and C. parapsilosis inhibiting by 89.3% and 80.7% of planktonic cell growth and 83.5% and 77.9% the biofilm formation, respectively. Furthermore, the mechanisms of action CpEO were elucidated by fluorescence. Scanning electron microscopy revealed damage to the cell membrane and pore formation, ROS overproduction, and Induction of apoptosis in candida cells. Our results reinforce the potential of CpEO as an effective alternative molecule of pharmaceutical interest.

Keywords

Croton; yeast; inhibition; chemical composition and action mechanisms

Subject

Biology and Life Sciences, Immunology and Microbiology

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